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Search Results: 1 - 10 of 40547 matches for " Wald'ma Sobrinho;Guimar?es "
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Congela??o de ovócitos desnudados ou n?o, maturos e imaturos de bovinos, utilizando o Etileno Glicol pelo método convencional
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Guimares, José Domingos;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000800014
Abstract: the objective of this study was to evaluate the cryopreservation of in vitro mature or immature oocytes by conventional method. the experiment was conducted using oocytes of cows' ovaries from slaughterhouse, distributed into six treatments: unfrozen oocytes with the cumulus oophorus cells (t1) and naked (t2) which were submitted to the process of miv, fiv and civ; immature oocytes, with the cumulus oophorus cells (t3) and naked (t4), which were submitted to the cryopreservation, unfrozen and the miv, fiv and civ; oocytes with the cumulus oophorus cells (t5) and naked (t6), which were submitted to in vitro maturation, cryopreservation, and fiv and civ. the oocytes were frozen by the conventional methods, dehydrated by the immersion in three solutions with 0.6; 1.2 and 1.8 mol l-1 of ethylene glycol (eg) during 5 minutes each phase. the thawing phase was done by the immersion in water-bath at 30 oc during 20 seconds, and so, the oocytes were re-hydrated in three phases (0.9 mol l-1 eg + 0.3 mol l-1 of sacarose; 0.3 l-1 of sacarose without eg and sacarose) for six minutes each one. the mainly ultrastructural changes in cryopreserved matured oocytes were prematurely released of cortical granules. however, the frozen immature and mature oocytes showed vacuolization and disappearance of cristae mitochondrial. the frozen immature oocytes showed the maturation rate of 82.5, 75.4, 9.2 and 5.8% for t1, t2, t3 and t4, respectively. the fecundation rate were 56.2, 0.0, 38.7, 8.6, 63.6 and 16.7% and from the cleavage were 36.3, 7.9, 0.4, 0.0, 0.0 and 0.0% for t1, t2, t3, t4, t5 and t6, respectively. morula and blastocyst were observed only for unfrozen and naked oocytes (t1) (34.5%). these results showed that the frozen protocols affect the viability of the oocytes.
Nova pipeta para insemina o intra-uterina em suínos
Diehl Gustavo Nogueira,Amaral Filha Wald'ma Sobrinho,Kummer Rafael,Koller Felipe
Ciência Rural , 2006,
Abstract: O objetivo deste trabalho foi avaliar o desempenho reprodutivo de 423 fêmeas suínas de ordem de parto 1 a 9 submetidas à insemina o intra-uterina (IAU), com um novo modelo de pipeta (T1) cuja extremidade n o é fixada na cérvix ou uma pipeta de IAU modelo Verona e que permite a fixa o da sua extremidade em espiral na cérvix (T2). Para comparar as duas pipetas, foi considerado o grau de dificuldade para realiza o das insemina es, o tempo necessário para realizá-las, a presen a de sangramento após a insemina o, a presen a de refluxo no momento da insemina o, as taxas de retorno ao estro (TR), de prenhez (TPR) e de parto ajustada (TPA), além do número de leit es nascidos (NT). As fêmeas de ambos os grupos foram inseminadas com doses de 1 bilh o de espermatozóides, em intervalos de 24 horas. A passagem do cateter de IAU através da cérvix foi possível em 95,9% das fêmeas, sem diferen a entre os tratamentos (P>0,05). Em pelo menos uma das insemina es, foi observado sangue no cateter, após a realiza o da IAU, em 20,6% das fêmeas do T1 e 15,2% das fêmeas do T2 (P=0,14). O tempo médio necessário para realizar a insemina o foi de 2,1 minutos para o T1 e 2,3 minutos para o T2 (P=0,26). O percentual de fêmeas com refluxo de sêmen no momento da insemina o foi maior (P=0,01) no T1 (8,4%) em compara o ao T2 (2,9%). N o houve diferen a (P>0,05) nas variáveis TR (8,0 e 4,8%), TPR (93,4 e 96,2%) e NT (12,4 e 12,7 leit es) entre T1 e T2, respectivamente. A TPA do T1 (90,6%) apresentou tendência (P=0,07) de ser inferior à do T2 (95,1%). No T1, as fêmeas primíparas apresentaram maior TR e menor TPA em compara o às pluríparas (P<0,05). Os resultados mostram que a nova pipeta pode ser utilizada sem prejuízos ao desempenho reprodutivo, em fêmeas pluríparas, mas sugerem cautela para sua utiliza o em fêmeas primíparas.
Nova pipeta para insemina??o intra-uterina em suínos
Diehl, Gustavo Nogueira;Amaral Filha, Wald'ma Sobrinho;Kummer, Rafael;Koller, Felipe;Bernardi, Mari Lourdes;Wentz, Ivo;Bortolozzo, Fernando Pandolfo;
Ciência Rural , 2006, DOI: 10.1590/S0103-84782006000100027
Abstract: the aim of this work was to evaluate the reproductive performance of 423 sows of 1 to 9 farrowings inseminated with two different intrauterine artificial insemination (iau) pipettes. the first group (t1) was formed by sows inseminated with a new pipette whose extremity was not fixed in the cervix. the second group (t2) was formed by sows inseminated with a veronaò pipette model, with its extremity fixed in the cervix. the degree of difficulty to perform the insemination, the time spent during the insemination, the presence of blood after insemination and of semen backflow during insemination were considered for the comparison of these pipettes. return to oestrus rate (ro), pregnancy rate (pr), adjusted farrowing rate (afr) and total litter size (tls) were also evaluated. all females were inseminated with 1 billion spermatozoa per dose applied at intervals of 24 hours. the catheter of iau was successfully inserted in 95.9% of females without difference between treatments (p>0.05). the presence of blood, in at least one insemination, was observed in 20.6% of t1 and 15.2% of t2 females (p=0.14). the average time to perform the insemination was 2.1 minutes for t1 and 2.3 minutes for t2 females (p=0.26). the percentage of females with semen backflow was significantly higher (p=0.01) in t1 (8.4%) compared to t2 (2.9%) group. there were no differences (p>0.05) in ro (8.0% and 4.8%), pr (93.4% and 96.2%) and tls (12.4 and 12.7 piglets) between t1 and t2 groups, respectively. afr of t1 (90.6%) tended (p=0.07) to be lower than that of t2 (95.1%). primiparous females of t1 showed higher ro and lower afr in comparison to pluriparous (p<0.05). the results indicate that the new pipette can be used in pluriparous females without compromising their reproductive performance. however, caution is necessary for the utilization of this new pipette in primiparous females.
Infec??o por Bordetella pertussis com hipertens?o pulmonar grave num recém -nascido com boa evolu??o clínica - Caso clínico
Soares,Sérgia; Rocha,Gustavo; Pissarra,Susana; Carri?o,Ana; Azevedo,Inês; Sim?es,Joana Sobrinho; Guimares,Hercília;
Revista Portuguesa de Pneumologia , 2008,
Abstract: in spite of the availability and widespread use of vaccines, pertussis is far from controlled. newborns and infants too young to be fully vaccinated, born from mothers with low antibody titers to bordetella pertussis, are highly susceptible to infection and at risk of severe disease and death. pertussis associated with pulmonary hypertension in the newborn is often fatal. the authors report a clinical case of severe pertussis-induced respiratory failure associated to severe pulmonary hypertension in a neonate successfully treated with sildenafil and inhaled nitric oxide.
Vitrifica??o de ovócitos desnudados ou n?o e previamente maturados in vitro
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Silva, Trícia Osório da;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000500004
Abstract: this study aimed at the evaluation of the effects from cryopreservation of bovine oocytes in vitro matured, by using ethylene glycol (eg) associated to trehalose and polyvinylpyrrolidone (pvp), of ovary oocytes of slaughtered cows, randomly assigned to three treatments. treatment 0 (t0 - control): oocytes that were desnuded and not vitrified. treatment 1 (t1): cryopreservation of in vitro matured oocytes with cumulus oophorus. tratamento 2 (t2): cryopreservation of in vitro matured desnuded oocytes. the percentage of recovered oocytes after cryopreservation and with normal morphology was different for vitrified oocytes (94.7 and 76.8%; 69.5 and 48.85% for t1 and t2, respectively). the main changes ultrastructural in vitrificated oocytes were prematurely released of cortical granules. later, all normal oocytes were fecundated and cultivated at 38.5oc in atmosphere with 5% co2 for seven days. the fecundation and cleavage rates for treatments were different (56.2, 41.7 and 12.5%; 36.3, 0.0 and 0.0%, for t0, t1 and t2, respectively). morulas and blastocysts were obtained only in t0 (34.5%). these results indicate that, the used protocols, for vitrification procedure is not indicated for cryopreservation of matured bovine oocytes.
Educa??o Ambiental: tecendo trilhas, escriturando territórios
Sampaio, Shaula Maíra Vicentini de;Guimares, Leandro Belinaso;
Educa??o em Revista , 2009, DOI: 10.1590/S0102-46982009000300017
Abstract: this paper inquires into the educational strategies used in the so-called interpretative tracks for environmental education. we ask, 'which territories are those receiving guides and students in environmental educational activities? which questions are made when walking? which are not made? which features turn a route into a track? which territories and tracks shape the ways for environmental education? to approach these questions the paper will focus on two different trials. first we have approached some aspects of the construction of an interpretative track in the community of pantano do sul in florianópolis, sc. about this track, meant to be performed with students from primary schools, above all, we inquire into the process culminating in designing its lines. in the second part, we have focused on the interpretative tracks in a state reserve near porto alegre, rs. to do so, processes of disciplining subjects, and discursive production of an untouched nature, are necessary. analyses conducted in the essay are in the challenged multi-faceted cultural studies field. in this field, work is carried out into readings on nature in the environmental education activities focused in this essay: the so-called interpretative tracks. furthermore, from the cultural studies we can inquire into the understandings on nature (and the way we act regarding it), from practices and discourses involved from planning and using an interpretative track to the moment when an educational work is developed in such spaces.
Some Morphological Aspects of Cylicocyclus brevicapsulatus (Nematoda: Cyathostominae) Parasite of Horse
Costa, Helio MA;Silva, Amalia VM;Guimares, Marcos P;
Memórias do Instituto Oswaldo Cruz , 2002, DOI: 10.1590/S0074-02762002000100014
Abstract: this paper describes some morphological aspects of cylicocyclus brevicapsulatus (ihle, 1920) (nematoda: cyathostominae) from equus caballus in brazil. the worms were studied using an optical microscope (measurements and illustrations) and a scanning electron microscope for a more detailed examination of the external morphology. the buccal capsule is very short, with a very thin wall, and the dorsal gutter is absent. other morphological aspects are described including measurement of the spicules and gubernaculum.
Some Morphological Aspects of Cylicocyclus brevicapsulatus (Nematoda: Cyathostominae) Parasite of Horse
Costa Helio MA,Silva Amalia VM,Guimares Marcos P
Memórias do Instituto Oswaldo Cruz , 2002,
Abstract: This paper describes some morphological aspects of Cylicocyclus brevicapsulatus (Ihle, 1920) (Nematoda: Cyathostominae) from Equus caballus in Brazil. The worms were studied using an optical microscope (measurements and illustrations) and a scanning electron microscope for a more detailed examination of the external morphology. The buccal capsule is very short, with a very thin wall, and the dorsal gutter is absent. Other morphological aspects are described including measurement of the spicules and gubernaculum.
Evaluation of solubility, disintegration, and dimensional alterations of a glass ionomer root canal sealer
Carvalho-Júnior Jacy Ribeiro,Guimares Luiz Fernando L.,Correr-Sobrinho Louren?o,Pécora Jesus D.
Brazilian Dental Journal , 2003,
Abstract: The aim of this study was to evaluate the glass ionomer cement Ketac-Endo (K) compared with Endofill (E), N-Rickert (N), and Sealer 26 (S) in terms of disintegration, solubility, and dimensional alteration properties, based on ADA Specification No 57. For dimensional alterations, 12-mm high cylindric specimens measuring 6 mm in diameter were prepared and left to stand for a period corresponding to three times the setting time. These specimens were immersed in 30 ml of deionized distilled water after measuring their length with a caliper. Thirty days later, the sample was removed from the container, dried and measured again for length to determine the percent of dimensional alteration. For solubility and disintegration, 1.5-mm thick cement samples measuring 20 mm in diameter were prepared and left to stand for a period corresponding to three times the setting time. The samples were weighed and immersed in 50 ml of deionized distilled water. After seven days, the samples were removed, dried and weighed again to determine the mass loss of each sample, expressed as percentage of original mass. This was considered to correspond to solubility and disintegration of cement. The results were: dimensional alteration: E (+0.14), K (-0.24), N (+0.23), S (+3.26); for disintegration and solubility: E (3.90), K (9.90), N (3.00), S (0.25). We concluded that the dimensional alteration of all cements conformed to ADA standards; Endofill and Ketac-Endo sealers presented higher values for disintegration and solubility than ADA recommendations. Obturating a root canal with a sealer that presents low disintegration and low contraction could minimize the penetration of fluids into the root canal system, thus sealing the space hermetically.
DETERMINA O INDIRETA DO ESTOQUE DE BIOMASSA E CARBONO EM POVOAMENTOS DE ACáCIA-NEGRA (Acacia mearnsii De Wild.)
Paulo Renato Schneider,César Augusto Guimares Finger,Valni Giacomelli Sobrinho,Paulo Sérgio Pigatto Schneider
Ciência Florestal , 2005,
Abstract: Este trabalho foi realizado com o objetivo de estimar o estoque de carbono em povoamentos equianeos de Acacia mearnsii De Wild., na regi o da Encosta Inferior do Sudeste, no Rio Grande do Sul, com o método de deriva o do volume em biomassa e carbono. As quantifica es dos componentes da biomassa e do carbono foram feitas em povoamentos com idade entre 4 e 8 anos. O método de deriva o do volume em biomassa e carbono mostrou-se eficiente na determina o do estoque de carbono, pois a diferen a relativa média foi de apenas 4,4%, quando considerada toda a amostragem e independência da idade dos povoamentos. A densidade básica média da madeira foi de 0,6, independente da idade dos povoamentos. A propor o de biomassa média entre o volume com casca pelo volume de folhas, ramos, serrapilheira e raízes foram de 0,59, independente da idade dos povoamentos. A concentra o média de carbono, independente da idade dos povoamentos, foi igual a 0,40. O estoque de carbono estimado pelo método de deriva o de volume e carbono, em povoamentos de 7 anos de idade, foi de 99,46 t ha-1 no índice de sítio 20, 82,98 t ha-1 no índice de sítio 16, e 46,13 t ha-1 no índice de sítio 12.
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