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Search Results: 1 - 10 of 1787 matches for " Volker Rusch "
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Throwing the dice for the diagnosis of vaginal complaints?
Andreas Schwiertz, David Taras, Kerstin Rusch, Volker Rusch
Annals of Clinical Microbiology and Antimicrobials , 2006, DOI: 10.1186/1476-0711-5-4
Abstract: We evaluated the misjudgement rate of the aetiology of vaginal complaints. A total of 220 vaginal samples from women with a vaginal complaint were obtained and analysed for numbers of total lactobacilli, H2O2-producing lactobacilli, total aerobic cell counts and total anaerobic cell counts including bifidobacteria, Bacteroides spp., Prevotella spp. Additionally, the presence of Atopobium vaginae, Gardnerella vaginalis, Candida spp. and Trichomonas vaginalis was evaluated by DNA-hybridisation using the PCR and Affirm VPIII Microbial Identification Test, respectively.The participating physicians diagnosed Bacterial vaginosis (BV) as origin of discomfort in 80 cases, candidiasis in 109 cases and mixed infections in 8 cases. However, a present BV, defined as lack of H2O2-lactobacilli, presence of marker organisms, such as G. vaginalis, Bacteroides spp. or Atopobium vaginae, and an elevated pH were identified in only 45 cases of the women examined. Candida spp. were detected in 46 cases. Interestingly, an elevated pH corresponded solely to the presence of Atopobium vaginae, which was detected in 11 cases.Errors in the diagnosis of BV and candida vulvovaginitis (CV) were high. Interestingly, the cases of misjudgement of CV (77%) were more numerous than that of BV (61%). The use of Amsel criteria or microscopy did not reduce the number of misinterpretations. The study reveals that the misdiagnosis of vaginal complaints is rather high.The microbiology of the vagina is complex, containing 109 bacterial colony forming units per gram of secretions and potentially dozens of species. It is mainly dominated by members of the genus Lactobacillus, which are capable of H2O2-production and lactic acid, thereby maintaining the generally acidic vaginal pH. Age, phase of the menstrual cycle, sexual activity, contraceptive choice, pregnancy, presence of necrotic tissue or foreign bodies, and use of hygienic products or antibiotics can disrupt this ecosystem. A disturbed vaginal microbiot
Molecular Tools for the Detection of Nitrogen Cycling Archaea
Antje Rusch
Archaea , 2013, DOI: 10.1155/2013/676450
Molecular Tools for the Detection of Nitrogen Cycling Archaea
Antje Rusch
Archaea , 2013, DOI: 10.1155/2013/676450
Abstract: Archaea are widespread in extreme and temperate environments, and cultured representatives cover a broad spectrum of metabolic capacities, which sets them up for potentially major roles in the biogeochemistry of their ecosystems. The detection, characterization, and quantification of archaeal functions in mixed communities require Archaea-specific primers or probes for the corresponding metabolic genes. Five pairs of degenerate primers were designed to target archaeal genes encoding key enzymes of nitrogen cycling: nitrite reductases NirA and NirB, nitrous oxide reductase (NosZ), nitrogenase reductase (NifH), and nitrate reductases NapA/NarG. Sensitivity towards their archaeal target gene, phylogenetic specificity, and gene specificity were evaluated in silico and in vitro. Owing to their moderate sensitivity/coverage, the novel nirB-targeted primers are suitable for pure culture studies only. The nirA-targeted primers showed sufficient sensitivity and phylogenetic specificity, but poor gene specificity. The primers designed for amplification of archaeal nosZ performed well in all 3 criteria; their discrimination against bacterial homologs appears to be weakened when Archaea are strongly outnumbered by bacteria in a mixed community. The novel nifH-targeted primers showed high sensitivity and gene specificity, but failed to discriminate against bacterial homologs. Despite limitations, 4 of the new primer pairs are suitable tools in several molecular methods applied in archaeal ecology. 1. Introduction Archaea have been detected in virtually all types of extreme and moderate environments. They play multiple ecological roles, colonizing certain newly emerging habitats [1, 2], interacting with animals such as corals [3, 4], sponges [5, 6], termites [7], or ruminants, forming part of microbe-microbe symbioses [8–10], and driving numerous processes in the biogeochemical C, N, S, and Fe cycles. In addition to relatively well-studied isolates of extremophilic or methanogenic Archaea, uncultured representatives have been detected by their 16S rRNA genes or by metabolic genes that classify their owners into the guilds of sulfate reducers, diazotrophs, ammonia oxidizers, or methanogens. Despite their widespread occurrence, a mere handful of nonmethanogenic Archaea has been isolated from moderate habitats [11–13]. While such isolates are indispensable for insight into archaeal ecophysiology, they have been recalcitrant to cultivation efforts, so that our current ecological research on mesophilic Archaea largely depends on cultivation-independent methods. Molecular
IRT models with relaxed assumptions in eRm: A manual-like instruction
Psychology Science Quarterly , 2009,
Abstract: Linear logistic models with relaxed assumptions (LLRA) as introduced by Fischer (1974) are a flexible tool for the measurement of change for dichotomous or polytomous responses. As opposed to the Rasch model, assumptions on dimensionality of items, their mutual dependencies and the distribution of the latent trait in the population of subjects are relaxed. Conditional maximum likelihood estimation allows for inference about treatment, covariate or trend effect parameters without taking the subjects' latent trait values into account. In this paper we will show how LLRAs based on the LLTM, LRSM and LPCM can be used to answer various questions about the measurement of change and how they can be fitted in R using the eRm package. A number of small didactic examples is provided that can easily be used as templates for real data sets. All datafiles used in this paper are available from http://eRm.R-Forge.R-project.org/
“Elude” – the making of an impossible game
Doris C. Rusch
E-Beratungsjournal , 2011,
Abstract: Right after the Games for Health Conference in 2009, Doris C. Rusch and Attila Ceranoglu decided to work on a game about depression: A game that actually intends to make the feelings of depression itself experientially tangible to players. They decided to use the internet and virtual reality to reach the digital natives easily, and to raise awareness of the difficulties and problems of depression amongst young people (and therefore become more willing to either seek help for themselves or others). But how to create such a game about depression? How to create a game that would be no fun? Rusch and Ceranoglu found the answers and now Elude“ is done!
Impacto de diferentes disturbios antrópicos sobre las comunidades de aves de bosques y matorrales de Nothofagus antarctica en el NO Patagónico
Lantschner,María V; Rusch,Verónica;
Ecolog?-a austral , 2007,
Abstract: to be able to plan productive uses of the land compatible with the conservation of the biodiversity, it is essential to evaluate the changes that they cause. the andean patagonic forests undergo different anthropogenic disturbances, like fires, grazing, wood extraction and substitution by exotic coniferous plantations. these activities cause changes on the vegetation and animal communities, and could be affecting the functioning of the forest ecosystems. in argentine patagonia, there is a growing opposition to productive activities, particularly to pine plantations, because of its supposed negative ecological consequences, however their impacts are unknown. the aim of this study was to evaluate in which measure different uses of the vegetation of the basin of foyel river (no argentine patagonia) cause changes on bird communities, especially on the functionally important and the endangered species. birds counts were conducted from november 2004 to march 2005 (summer) using the variable circular-plot method, in twenty four sites of nothofagus antarctica native forests and scrubs with different levels of grazing and wood extraction (closed, semiopened, opened), and in eight exotic plantations of pseudotsuga menziesii and pinus spp. although, the vegetation structure of each site was characterized, to identify structural elements of the forests that could be important for bird habitat. population densities of birds did not differ between vegetation types (25.4 ± 8.4 individuals/ha) associated to different disturbances. bird richness and diversity shannon index), however, were different among the vegetation types, in forestations both were similar to the undisturbed vegetation types, whereas they increased in vegetations with a medium intensity of wood extraction and grazing, and were highest in those sites whit a high intensity of wood extraction and grazing. a canonical correspondence analysis and an analysis of species composition similarity showed that there are two
Serotonin uptake rates in platelets from angiotensin II-induced hypertensive mice  [PDF]
Preeti Singh, Terry W. Fletcher, Yicong Li, Nancy J. Rusch, Fusun Kilic
Health (Health) , 2013, DOI: 10.4236/health.2013.54A005

Angiotensin II (Ang II) is a critical component of the reninangiotensin system that contributes to hypertension. Although platelets in blood from hypertensive subjects have an abnormal biological profile, it is unclear if circulating Ang II influences platelet aggregation or thrombus formation. One of the abnormalities presented to the platelets during hypertension is an elevated plasma concentration of serotonin (5-HT) caused by reduced 5-HT uptake secondary to loss of the 5-HT transporter (SERT) on the platelet plasma membrane. In the current study, we evaluated in vivo platelet function after 7 days of subcutaneous Ang II infusion to establish hypertension in mice and additionally assessed the biology of isolated platelets exposed to Ang II in vitro. The administration of Ang II elevated systolic blood pressure, but markers of platelet activation including P-selectin and PEJon/A staining were not changed. However, the aggregation response to collagen was reduced in isolated platelets from Ang II-infused mice, which also showed reduced 5-HT uptake by SERT. In vitro exposure of isolated platelets to Ang II also resulted in a loss of surface SERT associated with a reduced aggregation response to collagen. These abnormalities were reversed by increasing concentra tions of the Ang II receptor antagonist, valsartan. Interestingly, SERT KO mice failed to fully develop hypertension in response to Ang II infusion and isolated platelets from these animals were insensitive to the anti-aggregatory influence of Ang II. Thus, Ang II blunts the aggregation responses of platelets and the mechanism underlying this action may involve a loss of SERT on the platelet plasma membrane. The

Comparing Forests across Climates and Biomes: Qualitative Assessments, Reference Forests and Regional Intercomparisons
Carl F. Salk, Ulrich Frey, Hannes Rusch
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0094800
Abstract: Communities, policy actors and conservationists benefit from understanding what institutions and land management regimes promote ecosystem services like carbon sequestration and biodiversity conservation. However, the definition of success depends on local conditions. Forests' potential carbon stock, biodiversity and rate of recovery following disturbance are known to vary with a broad suite of factors including temperature, precipitation, seasonality, species' traits and land use history. Methods like tracking over-time changes within forests, or comparison with “pristine” reference forests have been proposed as means to compare the structure and biodiversity of forests in the face of underlying differences. However, data from previous visits or reference forests may be unavailable or costly to obtain. Here, we introduce a new metric of locally weighted forest intercomparison to mitigate the above shortcomings. This method is applied to an international database of nearly 300 community forests and compared with previously published techniques. It is particularly suited to large databases where forests may be compared among one another. Further, it avoids problematic comparisons with old-growth forests which may not resemble the goal of forest management. In most cases, the different methods produce broadly congruent results, suggesting that researchers have the flexibility to compare forest conditions using whatever type of data is available. Forest structure and biodiversity are shown to be independently measurable axes of forest condition, although users' and foresters' estimations of seemingly unrelated attributes are highly correlated, perhaps reflecting an underlying sentiment about forest condition. These findings contribute new tools for large-scale analysis of ecosystem condition and natural resource policy assessment. Although applied here to forestry, these techniques have broader applications to classification and evaluation problems using crowdsourced or repurposed data for which baselines or external validations are not available.
The Development of a Data-Centred Conceptual Reference Model for Strategic GRC-Management  [PDF]
Volker Nissen, Wolfgang Marekfia
Journal of Service Science and Management (JSSM) , 2014, DOI: 10.4236/jssm.2014.72007

Until now there are only few ideas for an integrated governance, risk and compliance (GRC) management available with these referring to the management process of GRC only. In literature, mainly specific questions at a detailed level, like the automation of different controls, are discussed in the GRC context. To be in the position to entirely realise benefit potentials (e.g. improvement of processes), it is necessary to have an integrated GRC-Management focusing on the strategic business objectives. Starting from the requirements, this article deals with general guidelines for strategic GRC-Management showing which aspects have to be considered in terms of an integral approach. On this basis, a data-centred reference model explicates the structural connections of GRC-related data, and lays the basis for the implementation in practice.

Portraying the Social Dimensions of Consulting with Structuration Theory  [PDF]
Christian Mauerer, Volker Nissen
Journal of Service Science and Management (JSSM) , 2014, DOI: 10.4236/jssm.2014.72010

In this paper, we argue that the consultant-client relationship is of central importance for consulting engagements. The paper therefore outlines the social dimensions that are inherent in the consulting system due to its characteristics that create social complexity. To gain further insights into the social interaction scheme and dynamics of consulting projects, a conceptualization based on an appropriate theoretical model is required. We propose to utilize the Structuration Theory for the compilation of the social context of consulting, as this provides a framework for incorporating the social determinants, focuses on actions of human-beings, and additionally allows the identification of interrelated dependencies of structure and actions.

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