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Search Results: 1 - 10 of 10083 matches for " Viviana Elizabeth;Contigiani "
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Age-related seroprevalence study for St. Louis encephalitis in a population from Cordoba, Argentina
SPINSANTI, Lorena Ivana;Ré, Viviana Elizabeth;DíAZ, María Pilar;CONTIGIANI, Marta Silvia;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2002, DOI: 10.1590/S0036-46652002000200001
Abstract: a serological screening was performed in 615 individuals aged 0-87 years, living in the city of cordoba, argentina to study the relationship between antibody prevalence for the sle virus and age. a 13.98% prevalence of neutralizing antibodies was obtained and its relation to age was significantly high (p = 0.045). the highest seroprevalence was noted on individuals over 60 years old (>20%), whereas no subject under 10 was seropositive for this virus. our results confirm that the agent is endemic in this area and neurological pathology studies should be performed on those individuals aged 60 since they represent the most susceptible group to sle virus.
Age-related seroprevalence study for St. Louis encephalitis in a population from Cordoba, Argentina
SPINSANTI Lorena Ivana,Viviana Elizabeth,DíAZ María Pilar,CONTIGIANI Marta Silvia
Revista do Instituto de Medicina Tropical de S?o Paulo , 2002,
Abstract: A serological screening was performed in 615 individuals aged 0-87 years, living in the city of Cordoba, Argentina to study the relationship between antibody prevalence for the SLE virus and age. A 13.98% prevalence of neutralizing antibodies was obtained and its relation to age was significantly high (p = 0.045). The highest seroprevalence was noted on individuals over 60 years old (>20%), whereas no subject under 10 was seropositive for this virus. Our results confirm that the agent is endemic in this area and neurological pathology studies should be performed on those individuals aged 60 since they represent the most susceptible group to SLE virus.
First detection of Rio Negro virus (Venezuelan equine encephalitis complex subtype VI) in Córdoba, Argentina
Pisano, María Belén;Spinsanti, Lorena Ivana;Díaz, Luis Adrián;Farías, Adrián Alejandro;Almirón, Walter Ricardo;Ré, Viviana Elizabeth;Contigiani, Marta Silvia;
Memórias do Instituto Oswaldo Cruz , 2012, DOI: 10.1590/S0074-02762012000100017
Abstract: rio negro virus (rnv) (venezuelan equine encephalitis subtype vi) circulates only in argentina; in northern provinces, isolates have been obtained from mosquitoes and rodents since 1980 and have been associated with acute febrile illness in humans. however, no studies of rnv have been performed in the central area of the country. we carried out molecular and serological detection of rnv in córdoba, a province of the central part of the country, in mosquitoes and humans, respectively. one mosquito pool tested positive for alphavirus rna by reverse transcriptase-nested polymerase chain reaction (rt-nested pcr). subsequent sequencing determined that this alphavirus grouped with rnv. serological studies detected antibodies to rnv in one human serum sample, which was obtained during the same period that rnv was detected using the aforementioned molecular methods. this is the first report of rnv circulation in the central area of argentina, indicating an expansion of its original distribution. these results highlight the importance of strengthening surveillance procedures in endemic areas, as well as in new regions where rnv may emerge.
Identification of hepatitis C virus subtype 2c by sequencing analysis in patients from Córdoba, Argentina
, Viviana;Contigiani, Marta;Yoshida, Clara Fumiko Tachibana;Lampe, Elisabeth;
Memórias do Instituto Oswaldo Cruz , 2007, DOI: 10.1590/S0074-02762007000800016
Abstract: in argentina, most information on hepatitis c virus (hcv) genotype distribution comes from studies carried out in buenos aires (east province). in order to identify hcv subtypes in central argentina, nucleotide sequencing of core region was performed in samples from 36 patients living in córdoba, the second most populated province of argentina. the sequence analysis identified subtype 2c as the most prevalent (50%), followed by subtype 1b (33%) and to a lesser extent by subtypes 1a (11%), 3a (3%) and 4a (3%). this is the first report of circulation of hcv subtype 2c in this region of argentina and also such high prevalence has never been found before in the genotype distribution of south america.
An indirect immunofluorescence assay to detect antibodies against St. Louis Encephalitis virus
SPINSANTI, Lorena;RE, Viviana;AGUILAR, Javier;CONTIGIANI, Marta;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2001, DOI: 10.1590/S0036-46652001000600008
Abstract: an in house indirect immmunofluorescence assay ( ifa ) in relation to neutralization (nt) reference test, was assessed as a fast and cheap method to carry out serological surveys for st. louis encephalitis virus (sle). sera obtained from 213 blood donors were analyzed by both tests. the prevalence of seropositivity obtained with ifa was lower than (30.98%) that observed on nt (41.78%). the relative specificity rate of ifa was 96.77% whereas its relative sensitivity rate was 69.66%. kappa index showed a good correlation between both tests. the results indicate that neutralization assay is still the serological test with the highest sensitivity and specificity relative rates for detecting antibodies against sle virus. nevertheless, the ifa could be useful as an alternative test in order to learn the circulation of the flavivirus genus in a certain area.
An indirect immunofluorescence assay to detect antibodies against St. Louis Encephalitis virus
SPINSANTI Lorena,RE Viviana,AGUILAR Javier,CONTIGIANI Marta
Revista do Instituto de Medicina Tropical de S?o Paulo , 2001,
Abstract: An in house indirect immmunofluorescence assay ( IFA ) in relation to neutralization (NT) reference test, was assessed as a fast and cheap method to carry out serological surveys for St. Louis Encephalitis virus (SLE). Sera obtained from 213 blood donors were analyzed by both tests. The prevalence of seropositivity obtained with IFA was lower than (30.98%) that observed on NT (41.78%). The relative specificity rate of IFA was 96.77% whereas its relative sensitivity rate was 69.66%. Kappa index showed a good correlation between both tests. The results indicate that neutralization assay is still the serological test with the highest sensitivity and specificity relative rates for detecting antibodies against SLE virus. Nevertheless, the IFA could be useful as an alternative test in order to learn the circulation of the Flavivirus genus in a certain area.
Evaluation of five screening tests licensed in Argentina for detection of hepatitis C virus antibodies
, Viviana;Gallego, Sandra;Trevi?o, Elena;Barbás, Gabriela;Domínguez, Claudia;Elbarcha, Osvaldo;Bepre, Héctor;Contigiani, Marta;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000300016
Abstract: this study was conducted to compare among the most recent generation of five screening tests licensed in argentina, in order to evaluate which of the tests has the best sensitivity for detection of antibodies against hepatitis c virus (hcv). the tests analyzed were: detect-hcv? (3.0) biochem immunosystems, canada; hepatitis c eia wiener lab., argentina; equipar hcv ab, italy; murex hcv 4.0, uk and serodia-hcv particles agglutination test, japan. the results obtained showed high discrepancy between the different kits used and show that some of the tests assessed have a low sensitivity for anti-hcv detection in both chronic infections and early seroconversion, and indicate that among the commercially available kits in argentina, murex hcv 4.0 (uk) and serodia-hcv particles agglutination test (japan) have the best sensitivity for hcv screening. although the sensitivity of the assays is the first parameter to be considered for blood screening, more studies should be carried out to assess the specificity of such assays.
Italo Birocchi e Antonello Mattone, Il diritto patrio tra diritto comune e codificazione
Ninfa Contigiani
Forum Historiae Iuris , 2007,
Abstract: In English.
Phylodynamics of Hepatitis C Virus Subtype 2c in the Province of Córdoba, Argentina
Viviana E. Ré,Andrés C. A. Culasso,Silvia Mengarelli,Adrián A. Farías,Fabián Fay,María B. Pisano,Osvaldo Elbarcha,Marta S. Contigiani,Rodolfo H. Campos
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019471
Abstract: The Hepatitis C Virus Genotype 2 subtype 2c (HCV-2c) is detected as a low prevalence subtype in many countries, except in Southern Europe and Western Africa. The current epidemiology of HCV in Argentina, a low-prevalence country, shows the expected low prevalence for this subtype. However, this subtype is the most prevalent in the central province of Córdoba. Cruz del Eje (CdE), a small rural city of this province, shows a prevalence for HCV infections of 5%, being 90% of the samples classified as HCV-2c. In other locations of Córdoba Province (OLC) with lower prevalence for HCV, HCV-2c was recorded in about 50% of the samples. The phylogenetic analysis of samples from Córdoba Province consistently conformed a monophyletic group with HCV-2c sequences from all the countries where HCV-2c has been sequenced. The phylogeographic analysis showed an overall association between geographical traits and phylogeny, being these associations significant (α = 0.05) for Italy, France, Argentina (places other than Córdoba), Martinique, CdE and OLC. The coalescence analysis for samples from CdE, OLC and France yielded a Time for the Most Common Recent Ancestor of about 140 years, whereas its demographic reconstruction showed a “lag” phase in the viral population until 1880 and then an exponential growth until 1940. These results were also obtained when each geographical area was analyzed separately, suggesting that HCV-2c came into Córdoba province during the migration process, mainly from Europe, which is compatible with the history of Argentina of the early 20th century. This also suggests that the spread of HCV-2c occurred in Europe and South America almost simultaneously, possibly as a result of the advances in medicine technology of the first half of the 20th century.
Caracterización molecular de cepas de Streptococcus pyogenes aisladas de cuadros invasores basada en el polimorfismo del regulón vir MOLECULAR CHARACTERIZATION Streptococcus pyogenes FROM INVASIVE INFECTIONS BASED ON VIR-REGULON POLYMORPHISM
M. TERESA ULLOA F.,VIVIANA SILVA A.,ELIZABETH PI?ONES A.,LORENA PORTE T.
Revista chilena de infectología , 2001,
Abstract: A pesar del aumento de infecciones invasoras por Streptococcus pyogenes: fasceitis necrozante (FN) y síndrome de shock tóxico (SST) en nuestro medio, su caracterización generalmente se limita a identificación de especie y prácticamente no existen estudios moleculares. La literatura describe diversos métodos para tipificar S. pyogenes (MLEE, PFGE y secuen-ciación de emm) que resultan complejos. Una alternativa es la técnica basada en el polimorfismo del regulón vir (emm, emm l, scp A, vir R) que es más sencilla y permite correlacionar los virRLFP con los serotipos M. Objetivo: caracterizar S. pyogenes aislados de cuadros invasores mediante vir-RFLP para establecer relaciones entre cepas locales y extranjeras. Materiales y métodos: se estudiaron 30 cepas de S. pyogenes aisladas de pacientes con cuadros invasores (SST/FN: 8; infecciones de piel y tejidos blandos: 13; infecciones de cavidades estériles: 9) mediante Long PCR-RFLP (amplificación regulón vir con partidores VUF y SBR, seguido de digestión con HaeIII). Resultados: 28/30 (93%) cepas presentaron regulones entre 4,2 - 7,8 Kb y dos entre 1,5 - 1,6 Kb. Se establecieron 14 patrones vir-RFLP y 3 grupos génicos. Dos grupos génicos presentaron patrones similares a los descritos para los serotipos M y se asociaron a una determinada evolución clínica: grupo I, similar a M1 y asociado a infecciones de cavidades estériles; grupo III, similar a M3 y asociado a SST/FN. El grupo II fue heterogéneo en relación a los serotipos, pero concentró las cepas aisladas de infecciones de piel y tejidos blandos. Conclusiones: Las cepas de S. pyogenes aisladas de cuadros severos presentan gran diversidad génica. Sin embargo, tienden a agruparse según serotipo y a asociarse a una determinada evolución clínica destacándose la asociación entre vir-RFLP, tipo serotipo M3 y SST/FN. Las dos cepas con amplicones "peque os" constituyen un hallazgo interesante, que requiere mayor investigación. Streptococcus pyogenes continues to be an epidemiological problem due to emergence of invasive infections such as necrotizing fasciitis (NF) and toxic shock syndrome (TSS). At present, S. pyogenes characterization is limited to species identification while molecular studies are restricted to a few specialized laboratories. The literature describes several methods of molecular typing (MLEE, PFGE and emm sequence) that are laborious and costly. Alternatively, a technique based on the vir-regulon polimorphism (emm, emm l, scpA, virR) provides a more simple approach and allows the differentiation of the M types based on virRFLP. The purpose of thi
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