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Search Results: 1 - 10 of 20973 matches for " Vijai Kumar "
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Molecular Assessment of 16S-23S rDNA Internal Transcribed Spacer Length Polymorphism of Aeromonas hydrophila  [PDF]
Vijai Singh, Indra Mani, Dharmendra Kumar Chaudhary
Advances in Microbiology (AiM) , 2012, DOI: 10.4236/aim.2012.22009
Abstract: Aeromonas hydrophila is an important bacterial pathogen which causes the hemorrhagic septicemia in fishes, amphibians and humans. Genetic relationships of diverse isolates of A. hydrophila were recovered from fish and water sources. These isolates were investigated by flanked region of 16S and 23S Ribosomal DNA (rDNA) internal transcribed spacer (ITS). Here we analyzed polymorphism of PCR-amplified 16S-23S rDNA ITS and their revealed band pattern consisting of one to four DNA fragments. The fragment size ranged from 500 to 1000 bp. The DNA band patterns revealed a considerable genetic diversity in interaspecies. The 750 bp size of band was common in all isolates of A. hydrophila except one Isolates AH21. The tRNA-Glu sequences were identified from 750 bp size of ITS region that could be used as strain level potential genotypic markers.
Modified CTAB Method for High Quality Genomic DNA Extraction from Medicinal Plants
Vijai Kumar Gupta
Pakistan Journal of Biological Sciences , 2011,
Abstract: Not Available
Extraction and Identification of Compound Derived from Ipomoea palmata Through Various Spectroscopic Techniques
Pratibha Singh,Vijai Kumar
Journal of Pharmacognosy and Phytochemistry , 2013,
Abstract: Extraction and separation of constituents from Ipomoea palmata have carried out adopting various chemical routes. Extracted and separated compounds have been identified by various spectroscopic techniques such as UV, and 1HNMR.Thease techniques revel that that two compound can be as 4-hydroxy-2-(3, 7- dimethyl-octa, 2-6, diphanyle) 6-methoxy acetophenones and 4,6-dihydroxy, -2-0-(4 hydroxy butyl ) acetophonone, whose melting points are 2250C and 2800C respectively. Elemental analysis and molecular weight determination (M+302) of compound –I (M.P. 2250C) have established that compound has molecular formula as C19H26O3 while compound II (MP. 2800C) depict its molecular formula as C12H16O5. These compound have potential application in various industries like medicine, beverages etc.
The Effect of Water Absorption on Mechanical Properties of Wood Flour/Wheat Husk Polypropylene Hybrid Composites  [PDF]
Pradeep Upadhyaya, Manoj Garg, Vijai Kumar, Ajay K. Nema
Materials Sciences and Applications (MSA) , 2012, DOI: 10.4236/msa.2012.35047
Abstract: The main objectives of this research were to study the effect of water absorption on mechanical properties of hybrid fiber reinforcement for polypropylene composites. The poor resistance towards water absorption is one of the draw- backs of natural fibers. Hybrid filler-polypropylene composites are subjected to water immersion tests in order to study the effects of water absorption on the mechanical properties. Composites specimens containing 30 phr and 40 phr fiber weight were prepared by melt blending process. Water absorption tests were conducted by immersion specimens in distilled water at room temperature for different time durations (24, 48, 72, 96, 120, 144, 168, 192 hours). The tensile, flexural and impact properties were investigated before and after water absorption. The percentage of moisture uptake increased as the increasing order of the filler loading due to the high cellulose content. The phase morphology of wood flour/wheat husk polypropylene hybrid composites were investigated by SEM, the dynamic mechanical properties of the composite are analyzed by DMA & wheat, wood filler interaction are analyzed by FT-IR.
Small bowel intussusception in a child following feeding jejunostomy: A rare case with review of literature  [PDF]
Basant Kumar, Richa Lal, Vijai Datta Upadhyaya, Banani Poddar
Open Journal of Pediatrics (OJPed) , 2012, DOI: 10.4236/ojped.2012.24046
Abstract: Jejuno-jejunal intussusception is a rare complication of feeding jejunostomy tube placement. A case of one year old child who underwent gastric pull-up for complicated tracheo-esophageal fistula had jejuno-jejunal intussusception induced by Witzel's feeding jejunostomy tube; is discussed with review of literature.
Progressive decline in yield and quality of sugarcane due to Sugarcane mosaic virus
Indian Phytopathology , 2012,
Modification of Poly(ether ether ketone) Polymer for Fuel Cell Application
Devesh Shukla,Yuvraj Singh Negi,Vijai Kumar
Journal of Applied Chemistry , 2013, DOI: 10.1155/2013/386903
Abstract: Polyelectrolyte membrane (PEM) is an important part of PEM fuel cell. Nafion is a commercially known membrane which gives the satisfactory result in PEM fuel cell operating at low temperature. Present research paper includes functionalization of Poly(ether ether ketone) (PEEK) polymer with phosphonic acid group. The functionalization was done with the help of nickel-based catalyst. Further, the polymer was characterized by the FTIR, EDAX, DSC, TGA, and 1H NMR, and it was found that PEEK polymer was functionalized with phosphonic acid group with good thermal stability in comparison to virgin PEEK. Finally, the thin films of functionalized polymer were prepared by solution casting method, and proton conductivity of film samples was measured by impedance spectra whose value was found satisfactory with good thermal stability in comparison to commercially available Nafion membrane. 1. Introduction PEEK is a semicrystalline aromatic polymer having high melting temperature (335°C) and very low or no solubility in ordinary solvents at room temperature. Sulfuric acid is the common solvent which dissolves and also sulfonates PEEK at room temperature. Dilute solutions at high temperatures can also be obtained in hydrofluoric acid, trifluoromethanesulfonic acid, dichlorotetrafluoroacetone monohydrate, phenol 1,2,4-trichlorobenzene, and benzophenone [1]. Therefore, modification of PEEK is typical to some extent but desired for many applications such as polymer electrolyte membrane fuel cell (PEMFC) where acid functionalized polymer is required. In case of PEEK, functionalization in polymer stage is mostly reported on sulfonation and nitration [2]. Phosphonation of polymer is one of the methods to modify their properties such as proton conductivity, mechanical and thermal stability, and in polymer stage it was reported by various groups. Alexandratos et al. [3] reported the phosphonation of polystyrene using PCl3 which gave the cross-linked polymer. However, Jakoby et al. [4] reported that the highly substituted phosphonic acid derivative of polyphenylsulfone (PPS) was achieved via the Pd-dibenzylideneacetone (dba) complexed catalyst in solvent diphenyl ether. Increase of reaction temperature from 90°C to 120°C also enhanced the substitution level. Bock et al. [5] reported phosphonation of the polysulfones (PSU) using nickel chloride and silylated phosphonates. The route was found less expensive and gave the halogen free product in comparison to palladium-based catalytic route. Lafitte and Jannasch [6] reported a phosphonation of polysulfones via the lithiation of
Schwannomas—Atypical Presentation and Challenges  [PDF]
R. Vijai, J. Ruban Kumar, R. Arihanth, Manoj Prabu, Narayanasami Bharath, Khalilur Rahman, Arcot Rekha
Case Reports in Clinical Medicine (CRCM) , 2019, DOI: 10.4236/crcm.2019.84011
Abstract: Introduction: Neurogenic tumors include schwannomas and neurofibro-mas. They occur often in the head and neck. However, their occurrence on the vagus is uncommon. A high index of suspicion is needed to order imaging in pelvic lesions that present atypically. Discussion: Schwannomas are hypointense on T1 and heterogeneously hyperintense on T2 on MRI. The histopathological appearance with Antoni type A and type B is typical of Schwannoma. Conclusion: Schwannomas are slow growing benign tumors that are separable from the parent nerve. Recurrence is uncommon after resection.
Genetically by Modified Crops: An Overview
Navin Srivastava,Vijai Kumar Gupta,Rajesh Pati,Rajarshi Kumar Gaur
Biotechnology , 2011,
Abstract: Genetically modified transgenic crops also referred to as a Genetically Modified (GM) crops are produced by when the genetic material of an organism (either DNA or RNA) is altered by use of recombinant DNA technology and the modification can be replicated and/or transferred to other cells or organisms. The recently understood recombinant DNA technology has potential, via genetic engineering, to incorporate a specific gene which controls a particular trait, without co-transfer of undesirable genes from donor species as occurs in conventional breeding. In recent year, the globally cultivated area of transgenics crops has increased more than 81 million hectares. Most GM crops grown today have been developed to resist certain insect pests. There are GM plants being developed today to produce specific vitamins, resist plant viruses and even produce products for medicinal uses.
Identification of regulatory elements in 16S rRNA gene of Acinetobacter species isolated from water sample
Shipra Srivastava,Vijai Singh,Vipin Kumar,Praveen Chandra Verma
Bioinformation , 2008,
Abstract: A bacterial strain, designated AcBz01, was isolated from a water sample collected from Gomti River, Lucknow, India, and identified using a molecular approach. On the basis of the bacterial 16S rRNA gene sequence phylogeny and comparison of this gene sequence with sequences in Ribosomal Database project II, evidence given in this study, it is proposed that isolate is closely related to members of the genus Acinetobacter. Identification and annotation of regulatory elements in the 16S rRNA gene and characterization of their interaction with the respective transcription factor can provide basis for better understanding of the mechanism of network of gene interaction of functionally related genes. The identification of such sites is relevant for locating promoter boundary of a gene and it also allows the prediction of specific gene expression pattern and response to disturbances in a known signaling pathway. Computational identification of regulatory elements and Transcription Factor with their binding sites in 16S rRNA gene of Acinetobacter sp. was performed using BPROM tool. We predicted the regulatory elements are TSS, -10 box, -35 box and three Transcription Factor (narP, ompR and fadR) with their binding sites in the upstream region of 16S rRNA gene of Acinetobacter sp. AcBz01. The GenBank accession number for 16S rRNA gene of Acinetobacter sp. AcBz01 is EU931637.
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