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Search Results: 1 - 10 of 469929 matches for " Vernadette A Simon "
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Quantitative miRNA Expression Analysis Using Fluidigm Microfluidics Dynamic Arrays
Jin Jang, Vernadette A Simon, Rod M Feddersen, Fariborz Rakhshan, Debra A Schultz, Michael A Zschunke, Wilma L Lingle, Christopher P Kolbert, Jin Jen
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-144
Abstract: We demonstrated highly correlated Ct values between multiplex and singleplex RT reactions in standard qPCR assays for miRNA expression using total RNA from A549 (R = 0.98; p < 0.0001) and H1299 (R = 0.95; p < 0.0001) lung cancer cell lines. The Ct values generated by the microfluidic technology (Fluidigm 48.48 dynamic array systems) resulted in a left-shift toward lower Ct values compared to those observed by ABI 7900 HT (mean difference, 3.79), suggesting that the microfluidic technology exhibited a greater sensitivity. In addition, we show that as little as 10 ng total RNA can be used to reliably detect all 48 or 96 tested miRNAs using a 96-multiplexing RT reaction in both FFPE and FF samples. Finally, we compared miRNA expression measurements in both FFPE and FF samples by qPCR using the 96.96 dynamic array and Affymetrix microarrays. Fold change comparisons for comparable genes between the two platforms indicated that the overall correlation was R = 0.60. The maximum fold change detected by the Affymetrix microarray was 3.5 compared to 13 by the 96.96 dynamic array.The qPCR-array based microfluidic dynamic array platform can be used in conjunction with multiplexed RT reactions for miRNA gene expression profiling. We showed that this approach is highly reproducible and the results correlate closely with the existing singleplex qPCR platform at a throughput that is 5 to 20 times higher and a sample and reagent usage that was approximately 50-100 times lower than conventional assays. We established optimal conditions for using the Fluidigm microfluidic technology for rapid, cost effective, and customizable arrays for miRNA expression profiling and validation.MicroRNAs (miRNAs) are short, single-stranded, noncoding RNAs that regulate gene expression by interacting with or inhibiting mRNA in both plants and animals [1-3]. To date, more than 800 human miRNAs have been identified and the total number is still increasing [4]. It is estimated that about two thirds of all
Multi-Platform Analysis of MicroRNA Expression Measurements in RNA from Fresh Frozen and FFPE Tissues
Christopher P. Kolbert, Rod M. Feddersen, Fariborz Rakhshan, Diane E. Grill, Gyorgy Simon, Sumit Middha, Jin Sung Jang, Vernadette Simon, Debra A. Schultz, Michael Zschunke, Wilma Lingle, Jennifer M. Carr, E. Aubrey Thompson, Ann L. Oberg, Bruce W. Eckloff, Eric D. Wieben, Peter Li, Ping Yang, Jin Jen
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0052517
Abstract: MicroRNAs play a role in regulating diverse biological processes and have considerable utility as molecular markers for diagnosis and monitoring of human disease. Several technologies are available commercially for measuring microRNA expression. However, cross-platform comparisons do not necessarily correlate well, making it difficult to determine which platform most closely represents the true microRNA expression level in a tissue. To address this issue, we have analyzed RNA derived from cell lines, as well as fresh frozen and formalin-fixed paraffin embedded tissues, using Affymetrix, Agilent, and Illumina microRNA arrays, NanoString counting, and Illumina Next Generation Sequencing. We compared the performance within- and between the different platforms, and then verified these results with those of quantitative PCR data. Our results demonstrate that the within-platform reproducibility for each method is consistently high and although the gene expression profiles from each platform show unique traits, comparison of genes that were commonly detectable showed that detection of microRNA transcripts was similar across multiple platforms.
Qualities of Effective Leadership in Higher Education  [PDF]
Simon A. Black
Open Journal of Leadership (OJL) , 2015, DOI: 10.4236/ojl.2015.42006
Abstract: The leadership of Higher Education institutions has been placed under increasing scrutiny since the 1980s with the expansion of student numbers, changes in funding for student places, increased marketization and student choice, and continuing globalisation of the sector. In this climate of change Higher Education institutions have been required to consider how to develop their leaders and what might be appropriate leadership behaviour to enable adaptation to these new circumstances. When the various paradigms of leadership encountered in the Higher Education sector are compared with established leadership theory and practice it is possible to identify further intricacies in the development of Higher Education leaders. Further consideration of practicalities within Higher Education identifies whether competence frameworks might assist in leadership development. An examination of a recently-developed comprehensive framework of leadership capabilities applied in an alternative sector leads to an evaluation as to whether the same constructs apply to the demands placed upon leaders in Higher Education. Analysis demonstrates that, with minor changes in terminology, the constructs remain appropriate and valid. The definitions of activities and behaviours offer insight into how Higher Education leaders could be developed and therefore form a potential framework of leadership capabilities for Higher Education.
Modeling Ocean Chlorophyll Distributions by Penalizing the Blending Technique  [PDF]
Mathias A. Onabid, Simon Wood
Open Journal of Marine Science (OJMS) , 2014, DOI: 10.4236/ojms.2014.41004

Disparities between the in situ and satellite values at the positions where in situ values are obtained have been the main handicap to the smooth modeling of the distribution of ocean chlorophyll. The blending technique and the thin plate regression spline have so far been the main methods used in an attempt to calibrate ocean chlorophyll at positions where the in situ field could not provide value. In this paper, a combination of the two techniques has been used in order to provide improved and reliable estimates from the satellite field. The thin plate regression spline is applied to the blending technique by imposing a penalty on the differences between the satellite and in situ fields at positions where they both have observations. The objective of maximizing the use of the satellite field for prediction was outstanding in a validation study where the penalized blending method showed a remarkable improvement in its estimation potentials. It is hoped that most analysis on primary productivity and management in the ocean environment will be greatly affected by this result, since chlorophyll is one of the most important components in the formation of the ocean life cycle.

Estimating Ocean Chlorophyll Using the Penalized Three Dimensional (3D) Blending Technique  [PDF]
Mathias A. Onabid, Simon Wood
Open Journal of Marine Science (OJMS) , 2018, DOI: 10.4236/ojms.2018.83021
The Thin Plate Regression Spline (TPRS) was introduced as a means of smoothing off the differences between the satellite and in-situ observations during the two dimensional (2D) blending process in an attempt to calibrate ocean chlorophyll. The result was a remarkable improvement on the predictive capabilities of the penalized model making use of the satellite observation. In addition, the blending process has been extended to three dimensions (3D) since it is believed that most physical systems exist in the three dimensions (3D). In this article, an attempt to obtain more reliable and accurate predictions of ocean chlorophyll by extending the penalization process to three dimensional (3D) blending is presented. Penalty matrices were computed using the integrated least squares (ILS) and integrated squared derivative (ISD). Results obtained using the integrated least squares were not encouraging, but those obtained using the integrated squared derivative showed a reasonable improvement in predicting ocean chlorophyll especially where the validation datum was surrounded by available data from the satellite data set, however, the process appeared computationally expensive and the results matched the other methods on a general scale. In both case, the procedure for implementing the penalization process in three dimensional blending when penalty matrices were calculated using the two techniques has been well established and can be used in any similar three dimensional problem when it becomes necessary.
Does Deming’s “System of Profound Knowledge” Apply to Leaders of Biodiversity Conservation?  [PDF]
Simon A. Black, Jamieson A. Copsey
Open Journal of Leadership (OJL) , 2014, DOI: 10.4236/ojl.2014.32006

The challenges of ecological and environmental change are significant and solutions remain largely under the influence of people and the decisions of governments, interest groups, national and local communities and individuals. Evidence suggests that despite 20 years of effort, conservation initiatives have failed to achieve the targets set for protecting biodiversity in the UN Convention on Biological Diversity 2010. A common factor influencing effectiveness of conservation work undertaken by the diverse mix of government, non-government and civil organisations is leadership. A command-and-control approach to leadership is commonly encountered in conservation and previous reviews suggest this as a major factor in ineffective conservation initiatives. This suggests that conservation leaders should consider a fundamentally different approach to leadership. We examine whether an alternative paradigm, Deming’s System of Profound Knowledge, offers a suitable new basis for leadership in biodiversity conservation. This “Systems Thinking” approach should encompass (i) an understanding of natural systems, (ii) a sense of how human behaviour is influenced, (iii) an understanding of how knowledge should inform decision-making and problem solving, and (iv) an understanding of variation in natural systems. Current paradigms of conservation management fail to address these four fundamentals and therefore do not represent the most effective way to manage conservation. Conversely, challenges and opportunities encountered in biodiversity conservation are well-aligned to a Systems Thinking approach. Leadership approaches defined in Deming’s “System of Profound Knowledge” offer significant positive impacts on biodiversity conservation achievement and provide lessons for leaders in other areas of human activity.

The Cluster Fe2Si18 as the New Quantum Bit System  [PDF]
K. V. Simon, A. V. Tulub
Computational Chemistry (CC) , 2015, DOI: 10.4236/cc.2015.32004
Abstract: Multiconfiguration quantum chemical calculation of geometry and electron properties of Fe2Si18 cluster indicates on the predictable change of spin states as a function of the excitation energy beginning from ground state with the total spin S = 4. The charges on the two Fe atoms are quite different as well as the charge distribution on the surrounding Si atoms. Nevertheless the total dipole moment of the cluster is a monotonically decreasing function of the excitation energy and it reaches practically zero value in the first singlet state in which the cluster represents a new version of a quibit system.
Fundamental Questions in Biology
Simon A. Levin
PLOS Biology , 2012, DOI: 10.1371/journal.pbio.0040300
Towards supporting scholarship in research by clinical pharmacy faculty
Pickard,A. Simon;
Pharmacy Practice (Granada) , 2006, DOI: 10.4321/S1885-642X2006000400007
Abstract: objective: the objective of this study was to assess the need for research support, faculty development, and topics of interest to clinical track pharmacy faculty that would facilitate scholarship in research. methods: a cross-sectional survey of pharmacy practice-based faculty at university of illinois at chicago (uic) completed via the web in november 2005. results: of 39 clinical track faculty respondents (48% response rate), 100% indicated they were interested in being co-investigator or 77% lead investigator on a research grant proposal. the majority of respondents expressed "a lot" or "extreme" interest in receiving methodological guidance and administrative support in order to pursue research interests. the greatest interest in research support services related to sample size calculations, selection of appropriate statistical tests, grant writing, and writing for journals. barriers to research cited by faculty included lack of confidence in ability, the need for balancing responsibilities, and reward for efforts. suggestions included the creation of specific research interest groups, research seminars, formal mentoring and statistical support services. conclusions: clinical-track faculty are interested in research-related scholarship but typically lack the confidence or skills to lead research. while this study was limited to uic clinical faculty, uic faculty are attracted from colleges of pharmacy across north america and it is notable that such barriers can be quickly identified using a brief web-based survey in order to inform a plan that provides resources and support for research by clinical pharmacy faculty.
心理学报 , 1991,
Abstract: 今天我要谈的是这几年来认知心理学的一些新进展。我1983年在北大讲演的时候谈到认知心理学的一些情况,这个讲演后来被整理出一本书,即《人类的认知》。因此,我想谈谈在那之后有了哪些新的发展,主要是近一个阶段认知科学、认知心理学的新情况。我
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