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Search Results: 1 - 10 of 212485 matches for " Vera L.; "
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Effect of Tityus serrulatus venom on cytokine production and the activity of murine macrophages
Vera L. Petricevich
Mediators of Inflammation , 2002, DOI: 10.1080/09629350210308
Abstract: The purpose of this study was to investigate the effects of Tityus serrulatus venom (TSV) on murine peritoneal macrophages evaluated in terms of activation. The effects of crude TSV were analysed by detection of cytokines, oxygen intermediate metabolites (H2O2) and nitric oxide (NO) in supernatants of peritoneal macrophages. Several functional bioassays were employed including an in vitro model for envenomating: cytotoxicity of TSV was assessed using the lyses percentage. Tumor necrosis factor (TNF) activity was assayed by measuring its cytotoxic activity on L-929 cells, and interleukin-6 (IL-6) and interferon-γ (IFN-γ) were assayed by enzyme-linked immunosorbent assay, whereas NO levels were detected by Griess colorimetric reactions in culture supernatant of macrophages incubated with TSV and subsequently exposed to either lipopolysaccharide or IFN-γ. Incubation of macrophages with TSV increased production of IL-6 and IFN-γ in a dose-dependent manner. TNF production was not detected in supernatants treated with TSV at any concentration. The increase in IL-6 secretion was not associated with concentration-dependent cytoxicity of TSV on these cells. These data suggest that the cytotoxicity does not appear to be the main cause of an increased cytokine production by these cells. Although NO is an important effector molecule in macrophage microbicidal activity, the inducing potential of the test compounds for its release was found to be very moderate, ranging from 125 to 800 mM. Interestingly, NO levels of peritoneal macrophages were increased after IFN-γ. Moreover, NO production had an apparent effect on macrophage activity. The results obtained here also shown that the TSV induces an important elevation in H2O2 release. These results combined with NO production suggest that TSV possesses significant immunomodulatory activities capable of stimulating immune functions in vitro.
Balance Between Pro- and Anti-Inflammatory Cytokines in Mice Treated With Centruroides noxius Scorpion Venom
Vera L. Petricevich
Mediators of Inflammation , 2006, DOI: 10.1155/mi/2006/54273
Abstract: CSV consists of a very complex of molecules and demonstrates significant cellular activities capable of stimulating immune functions in vivo. The purpose of this study was to analyze the effects of CSV on sex, weight, route of injection and the balance of pro- and anti-inflammatory cytokines in mice. The susceptibility and route of injection were analyzed by lethal (LD50) determination. The effects of CSV were also analyzed in blood from immunized mice using detection by means of antibodies and mediators production. Several functional bioassays were employed: TNF activity was assayed by measuring its cytotoxic activity in L929 cells, and other cytokines were assayed by enzyme-linked immunosorbent assay, whereas nitric oxide levels were detected by Griess colorimetric reactions in sera from BALB/c mice. After injecting subcutaneously, the LD50 presented an increase of the CSV correlation and similar levels of susceptibility were obtained for female and male from BALB/c mice. Significant differences were observed in the time-course of cytokine levels. The balance of pro- and anti-inflammatory cytokines TNF/IL-10 and IL-6/IL-10 ratios were significantly higher in injected mice group when compared with those obtained for non-injected group. The CSV is poor in antigenic composition and it is difficult to get antibodies specific to neutralizing the lethal factor. The effect of immunization with 0.5 LD50 of CSV on the balance of pro- and anti-inflammatory cytokines was measured. The maximum levels of TNF and IL-6, IFN-γ and NO were observed on days 7 and 21 after immunization, respectively. IL-10 levels peaked between days 21 and 28 after immunization with CSV. With respect, to balance of pro- and anti-inflammatory cytokines it was possible to observe that negative correlation between serum levels of IL-6/IL-10 and TNF/IL-10 exists. These ratios may possibly reflect the balance of pro- and anti-inflammatory cytokines in serum, which may by manifested in the inflammatory status during the envenoming processes. In conclusion, an increase in the serum levels of TNF and IL-6 may be a useful marker for scorpion envenomation.
Scorpion Venom and the Inflammatory Response
Vera L. Petricevich
Mediators of Inflammation , 2010, DOI: 10.1155/2010/903295
Abstract: Scorpion venoms consist of a complex of several toxins that exhibit a wide range of biological properties and actions, as well as chemical compositions, toxicity, and pharmacokinetic and pharmacodynamic characteristics. These venoms are associated with high morbility and mortality, especially among children. Victims of envenoming by a scorpion suffer a variety of pathologies, involving mainly both sympathetic and parasympathetic stimulation as well as central manifestations such as irritability, hyperthermia, vomiting, profuse salivation, tremor, and convulsion. The clinical signs and symptoms observed in humans and experimental animals are related with an excessive systemic host inflammatory response to stings and stings, respectively. Although the pathophysiology of envenomation is complex and not yet fully understood, venom and immune responses are known to trigger the release of inflammatory mediators that are largely mediated by cytokines. In models of severe systemic inflammation produced by injection of high doses of venom or venoms products, the increase in production of proinflammatory cytokines significantly contributes to immunological imbalance, multiple organ dysfunction and death. The cytokines initiate a cascade of events that lead to illness behaviors such as fever, anorexia, and also physiological events in the host such as activation of vasodilatation, hypotension, and increased of vessel permeability.
Sala de aula e teceduras subjetivas
Blum, Vera Lúcia;
Educa??o e Pesquisa , 2008, DOI: 10.1590/S1517-97022008000300009
Abstract: the objective of this article is to think the task of teaching under the focus of the vicissitudes to which the teacher-student relationship is subjected, which steer pupils and teachers away from the ideals of order and harmony. with the purpose of analyzing the intersubjective flows in the classroom, aspects of group psychodynamics are investigated with the use of the psychoanalytic notions of basic assumption group and of projective identification. with this procedure, we want at the same time to probe to which extent notions that are efficacious in psychoanalytic clinic can be useful to analyze and to understand situations that belong to the field of education, and in particular the classroom, seen here as a space for the production of psychic inter-occurrences. we try to think the intersubjective space according to a logic that considers the creation of a third party in teaching and learning. we introduce the notion of a "third voice" (ogden) to denote the creation and re-creation of the intersubjective other with various effects on the subjective constitutions. the identitary stiffening, along with the attending disdain and intolerance to difference, can be a form of reacting to that which escapes from control and predictability, thereby weakening the transformational movements implicated in the process of learning.
Success and failure of the sociology of culture? Bringing the arts back
Zolberg, Vera L.;
Sociedade e Estado , 2005, DOI: 10.1590/S0102-69922005000200004
Abstract: this articles brings up the most recent, internal discussions among researchers in the field of the sociology of arts, more precisely the thiking of experts who participated in a forum of culture organized in 2001. in her writing, vera zolberg accentuates, overall, the need for sociology of art to retain both its humanistic and scientific roots. she reminds that sociology of culture has developed from the sociology of art in recent decades. next, she summarizes the tendencies and interests of researchers in the forum, by showing the fluidity of its possibilities and creative capacities. she mentions the most recent production in the field, proving that aesthetic elements play a crucial part in the sociological studies about the arts. according to vera zolberg, it is time to bring the arts back.
Rectoscopía en el síndrome diarreico del lactante
Revista chilena de pediatría , 1955,
Recetas no. Lecciones si.
Vera Grabe L
Revista de Estudios Sociales , 1998,
Vera L. Can?ado
Revista Gest?o & Tecnologia , 2010,
Abstract: A presente edi o da Revista Gest o e Tecnologia é dedicada à área de Gest o de Pessoas, trazendo contribui es diferenciadas e complementares, que enriquecem a compreens o do tema no contexto organizacional.
Vera L. Can?ado
Revista Gest?o & Tecnologia , 2011,
Vera L. Can?ado
Revista Gest?o & Tecnologia , 2011,
Abstract: Expediente
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