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Search Results: 1 - 10 of 488093 matches for " Vera Lúcia S.; "
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Three decades of studies on chromosomal polymorphism of Drosophila willistoni and description of fifty different rearrangements
Rohde, Claudia;Valente, Vera Lúcia S.;
Genetics and Molecular Biology , 2012, DOI: 10.1590/S1415-47572012000600012
Abstract: drosophila willistoni (insecta, diptera) is considered a paradigm for evolutionary studies. their chromosomes are characterized by multiple paracentric inversions that make it hard to identify and describe chromosomal poly-morphisms. in the present report we attempted to systematize the description of all the 50 inversions found in the last three decades, since we have been studying the chromosomes of several individuals of 30 different populations, including the one used in the genome sequencing project (gd-h4-1). we present the photographic register of 11 arrangements in the left arm of the x chromosome (xl), eight in the right arm (xr), 10 in the left arm of chromosome ii (iil), eight in its right arm (iir) and 13 in chromosome iii. this information also includes their breakpoints on the reference photomap. a clear geographic difference was detected in xl and xr, with different fixed arrangements depending on the origin of the population studied. through the comparison of all x arrangements it was possible to infer the putative ancestral arrangements, i.e., those related to all the remaining arrangements through the small number of inversions that occurred in the past, which we will call xl-a and xr-a. in the autosomes (iil/iir and iii), fixed inversions were detected, but most are segregating in different frequencies along the geographical distribution of the d. willistoni populations.
The Influence of Window Length Analysis on the Time and Frequency Domain of Mechanomyographic and Electromyographic Signals of Submaximal Fatiguing Contractions  [PDF]
Guilherme Nogueira-Neto, Eduardo Scheeren, Eddy Krueger, Percy Nohama, Vera Lúcia S. N. Button
Open Journal of Biophysics (OJBIPHY) , 2013, DOI: 10.4236/ojbiphy.2013.33021
Abstract:

Mechanomyography (MMG) acquires the oscillatory waves of contracting muscles. Electromyography (EMG) is a tool for monitoring muscle overall electrical activity. During muscle contractions, both techniques can investigate the changes that occur in the muscle properties. EMG and MMG parameters have been used for detecting muscle fatigue with diverse test protocols, sensors and filtering. Depending on the analysis window length (WLA), monitoring physiological events could be compromised due to imprecision in the determination of parameters. Therefore, this study investigated the influence of WLA variation on different MMG and EMG parameters during submaximal isometric contractions monitoring MMG and EMG parameters. Ten male volunteers performed isometric contractions of elbow joint. Triaxial accelerometer-based MMG sensor and EMG electrodes were positioned on the biceps brachii muscle belly. Torque was monitored with a load cell. Volunteers remained seated with hip and elbow joint at angles of 110° and 90°, respectively. The protocol consisted in maintaining torque at 70% of maximum voluntary contraction as long as they could. Parameter data of EMG and the modulus of MMG were determined for four segments of the signal. Statistical analysis consisted of analyses of variance and Fishers least square differences post-hoc test. Also, Pearson’s correlation was calculated to determine whether parameters that monitor similar physiological events would have strong correlation. The modulus of MMG mean power frequency (MPF) and the number of crossings in the baseline could detect changes between fresh and fatigued muscle with 1.0 s WLA. MPF and the skewness of the spectrum (μ3), parameters related to the compression of the spectrum, behaved differently when monitored with a triaxial MMG sensor. The EMG results show that for the 1.0 s and 2.0 s WLAs have normalized RMS difference with fatigued muscle and that there was strong correlation between parameters of different domains.

O papel da aten??o na fisioterapia neurofuncional
Bertoldi, Andréa Lúcia Sério;Israel, Vera Lúcia;Ladewig, Iverson;
Fisioterapia e Pesquisa , 2011, DOI: 10.1590/S1809-29502011000200016
Abstract: this study presents a theoretical review of attention in the creation of strategies of movement control from the perspective of the systemic paradigm applied to motor behavior and its relationship with the motor control approach in neurofunctional physical therapy. the following databases were consulted medline, pubmed, lilacs, scielo, and pedro, including articles from 1990 to 2009. research has shown the understanding of attention as a mediator agent between a subject and the environment in the process of self-organization of organic sub-systems during the realization of a motor task. similarities between this theoretical perspective of attention and the assumptions that support the motor control in the neurofunctional physical therapy approach have been identified, evidencing paths of scientific investigation for a better comprehension about the attention role on neuromotor system adaptation of individuals with neurological lesions and its efficiency to promote human functional movement.
Distribution and conservation of the transposable element gypsy in drosophilid species
Herédia, Fabiana;Loreto, Elgion Lúcio S.;Valente, Vera Lúcia S.;
Genetics and Molecular Biology , 2007, DOI: 10.1590/S1415-47572007000100023
Abstract: in an attempt to understand the dynamics of transposable elements (t's) in the genome of host species, we investigated the distribution, representativeness and conservation of dna sequences homologous to the drosophila melanogaster gypsy retrotransposon in 42 drosophilid species. our results extended the knowledge about the wide distribution of gypsy in the genus drosophila, including several neotropical species not previously studied. the gypsy-like sequences showed high divergence compared to the d. melanogaster gypsy element. furthermore, the conservation of the restriction sites between gypsy sequences from phylogenetically unrelated species pointed to a more complex evolutionary picture, which includes the possibility of the horizontal transfer events already described for this retrotransposon.
Efeito e modo de a??o das bacteriocinas produzidas por Lactococcus lactis subsp. lactis ITAL 383, ATCC 11454 e CNRZ 150 contra Listeria innocua LIN 11
MORENO, Izildinha;LERAYER, Alda L. S.;BALDINI, Vera Lúcia S.;LEIT?O, Mauro F. F.;
Ciência e Tecnologia de Alimentos , 1999, DOI: 10.1590/S0101-20611999000100007
Abstract: the effect and mode of action of the bacteriocin produced by l. lactis subsp. lactis ital 383 and cnrz 150 are similar to the nisin produced by l. lactis subsp. lactis atcc 11454. it was clearly bactericidal, and caused lysis of a strain of l. innocua lin 11 detected by the decrease of absorbance values and the cell viability. their lethal effect was considerably higher during the logarithmic growth when compared to the stationary phase. adsorption developed rapidly and was influenced by the ph value of the suspension medium. maximum adsorption was observed at ph 6,0 and immediately after initial contact and loss at ph 2,0.
Efeito e modo de a o das bacteriocinas produzidas por Lactococcus lactis subsp. lactis ITAL 383, ATCC 11454 e CNRZ 150 contra Listeria innocua LIN 11
MORENO Izildinha,LERAYER Alda L. S.,BALDINI Vera Lúcia S.,LEIT?O Mauro F. F.
Ciência e Tecnologia de Alimentos , 1999,
Abstract: O efeito e o modo de a o das bacteriocinas produzidas por L. lactis subsp. lactis ITAL 383 e CNRZ 150 s o similares à nisina de L. lactis subsp. lactis ATCC 11454. Estas bacteriocinas apresentaram um modo de a o bactericida, causando a lise de células de L. innocua LIN 11, associada ao decréscimo da absorbancia e da viabilidade celular. O efeito letal foi maior para células em fase exponencial comparativamente à fase estacionária de crescimento. A adsor o dessas bacteriocinas às células de L. innocua LIN 11 foi muito rápida e influenciada pelo pH do meio de suspens o; adsor o máxima foi verificada a pH 6,0 e logo após o contato inicial. Perda completa de adsor o ocorreu em pH 2,0.
Nuclear phenotype changes after heat shock in Panstrongylus megistus (Burmeister)
Garcia, Simone L;Mello, Maria Luiza S;Rodrigues, Vera Lúcia CC;Garcia, Nancy L;
Memórias do Instituto Oswaldo Cruz , 2000, DOI: 10.1590/S0074-02762000000200024
Abstract: the nuclear phenotypes of malpighian tubule epithelial cells of male nymphs of the blood-sucking insect, panstrongylus megistus, subjected to short- and long-duration heat shocks at 40oc were analyzed immediately after the shock and 10 and 30 days later. normal nuclei with a usual heterochromatic body as well as phenotypes indicative of survival (unravelled heterochromatin, giants) and death (apoptosis, necrosis) responses were observed in control and treated specimens. however, all nuclear phenotypes, except the normal ones, were more frequent in shocked specimens. similarly altered phenotypes have also been reported in triatoma infestans following heat shock, although at different frequencies. the frequency of the various nuclear phenotypes observed in this study suggests that the forms of cell survival observed were not sufficient or efficient enough to protect all of the malpighian tubule cells from the deleterious effects of stress. in agreement with studies on p. megistus survival following heat shock, only long-duration shock produced strongly deleterious effects.
Changes in nuclear phenotypes following cold shock in Panstrongylus megistus (Burmeister)
Garcia, Simone L;Mello, Maria Luiza S;Garcia, Nancy L;Rodrigues, Vera Lúcia CC;
Memórias do Instituto Oswaldo Cruz , 2000, DOI: 10.1590/S0074-02762000000600027
Abstract: the nuclear phenotypes of malpighian tubule epithelial cells of 5th instar male nymphs of the blood-sucking insect panstrongylus megistus were studied immediately after a short (1 h) cold shock at 0oc, and 10 and 30 days later. the objective was to compare the responses to a cold shock with those known to occur after hyperthermia in order to provide insight into the cellular effect of cold in this species. nuclei which usually exhibited a conspicuous y chromosome chromocenter were the most frequent phenotype in control and treated specimens. phenotypes in which the heterochromatin was unravelled, or in which there was nuclear fusion or cell death were more abundant in the shocked specimens. most of the changes detected have also been found in heat-shocked nymphs, except for nuclear fusion which generates giant nuclei and which appeared to be less effective or necessary than that elicited after heat shock. since other studies showed that a short cold shock does not affect the survival of more than 14% of 5th instar nymphs of p. megistus with domestic habit and can induce tolerance to a prolonged cold shock, heat shock proteins proteins are probably the best candidates for effective protection of the cells and the insects from drastic damage caused by low temperature shocks.
Changes in nuclear phenotypes following cold shock in Panstrongylus megistus (Burmeister)
Garcia Simone L,Mello Maria Luiza S,Garcia Nancy L,Rodrigues Vera Lúcia CC
Memórias do Instituto Oswaldo Cruz , 2000,
Abstract: The nuclear phenotypes of Malpighian tubule epithelial cells of 5th instar male nymphs of the blood-sucking insect Panstrongylus megistus were studied immediately after a short (1 h) cold shock at 0oC, and 10 and 30 days later. The objective was to compare the responses to a cold shock with those known to occur after hyperthermia in order to provide insight into the cellular effect of cold in this species. Nuclei which usually exhibited a conspicuous Y chromosome chromocenter were the most frequent phenotype in control and treated specimens. Phenotypes in which the heterochromatin was unravelled, or in which there was nuclear fusion or cell death were more abundant in the shocked specimens. Most of the changes detected have also been found in heat-shocked nymphs, except for nuclear fusion which generates giant nuclei and which appeared to be less effective or necessary than that elicited after heat shock. Since other studies showed that a short cold shock does not affect the survival of more than 14% of 5th instar nymphs of P. megistus with domestic habit and can induce tolerance to a prolonged cold shock, heat shock proteins proteins are probably the best candidates for effective protection of the cells and the insects from drastic damage caused by low temperature shocks.
Nuclear phenotype changes after heat shock in Panstrongylus megistus (Burmeister)
Garcia Simone L,Mello Maria Luiza S,Rodrigues Vera Lúcia CC,Garcia Nancy L
Memórias do Instituto Oswaldo Cruz , 2000,
Abstract: The nuclear phenotypes of Malpighian tubule epithelial cells of male nymphs of the blood-sucking insect, Panstrongylus megistus, subjected to short- and long-duration heat shocks at 40oC were analyzed immediately after the shock and 10 and 30 days later. Normal nuclei with a usual heterochromatic body as well as phenotypes indicative of survival (unravelled heterochromatin, giants) and death (apoptosis, necrosis) responses were observed in control and treated specimens. However, all nuclear phenotypes, except the normal ones, were more frequent in shocked specimens. Similarly altered phenotypes have also been reported in Triatoma infestans following heat shock, although at different frequencies. The frequency of the various nuclear phenotypes observed in this study suggests that the forms of cell survival observed were not sufficient or efficient enough to protect all of the Malpighian tubule cells from the deleterious effects of stress. In agreement with studies on P. megistus survival following heat shock, only long-duration shock produced strongly deleterious effects.
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