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Search Results: 1 - 10 of 400780 matches for " Vegad M.M. "
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OCCURRENCE AND DETECTION OF EXTENDED SPECTRUM β-LACTAMASE AND AmpC β-LACTAMASE IN CLINICAL ISOLATES OF Pseudomonas aeruginosa AND Acinetobacter baumanii BY INHIBITOR BASED METHOD
Trivedi G.R.,Soni S.T., Vegad M.M.,Yadav K.S.
International Journal of Microbiology Research , 2012,
Abstract: Background & Objective: Pseudomonsa aeruginosa and Acinetobacter baumanii are common non-fermenters which have emerged as the most common opportunistic pathogens in recent years. Persistent exposure of Pseudomonas aeruginosa and Acinetobacter baumanii to β-lactam antibiotics leads to acquired resistance through mutation and over production of various enzymes which also include AmpC or class C β-lactamases and extended spectrum β-lactamase (ESBL). For clinical microbiologists, detection of ESBL and AmpC-mediated resistance together poses a problem because the phenotypic tests may be misleading; resulting in misreporting and treatment failures.Methods: A total number of 94 consecutive, non-repetitive, imipenem sensitve clinical isolates of Pseudomonas aeruginosa (n=64) and Acinetobacter baumanii (n=30) obtained over a period of 6 months, were screened for β-lactamase production by nitrocefin disc and production of ESBL and AmpC β-lactamase is detected by Inhibitor based test.Results: A total of 50 out of 94 isolates were positive for β-lactamase production; of which 17 (15.98%) and 22(20.68%) were ESBL and AmpC β-lactamase producers respectively.Conclusion: The inhibitor based method is useful for detection of ESBL and AmpC β-lactamase and helpful to differentiate ESBL from AmpC producers. As high incidence of ESBL and AmpC β-lactamase production in gram negative isolates is alarming and urgent actions needs to be taken for therapeutic and infection control measure. This is only possible if correct detection of ESBL and AmpC β-lactamase is done in clinical laboratory.
PREVALENACE OF VANCOMYCIN RESISTANT ENTEROCOCCI IN TERTIARY CARE HOSPITAL, WESTERN, INDIA
Modi G.B., Soni S.T., Patel K.J., Goswami H.M. and Vegad M.M.
International Journal of Microbiology Research , 2012,
Abstract: Introduction: Vancomycin Resistant Enterococci (VRE) is fast emerging pathogen causes serious & life threatening hospital borne infec-tions. The limitation in therapeutic options has resulted in the development of new drugs such as Quinupristin/ Dalfopristin, Linezolid & Tigecycline.Objectives: This study signals the emergence of VRE in this hospital and also highlights the importance of screening for VRE in isolated enterococci from various clinical samples.Materials and Methods: From July 2010 to July 2011, a total of 250 enterococcal isolates were identified and speciated by standard bio-chemical tests. Antibiotic sensitivity was carried out by disc diffusion test as per CLSI guidelines. Minimum Inhibitory Concentration (MIC) of all the isolates were done by Vancomycin Ezy MICTM Strip ranging from 0.016 μg/ml to 256 μg/ml.Results: Out of the 250 Enterococcus species, 4% (10) isolates were resistant to Vancomycin by disc diffusion & Vancomycin MIC test in which 8 were E. faecium and 2 were E. faecalis. Out of 10 VRE, 7 were resistant and 3 were sensitive to Teicoplanin, so they were of VanA and VanB phenotype respectively. The Vancomycin MIC for seven of these isolates is more than 256 μg/ml. 1.2% showed intermediate resistance and 2.8% high level resistance to vancomycin by MIC according to CLSI guidelines.Conclusion: All laboratories should have effective detection methods for Vancomycin resistance, which will be helpful in treatment of VRE, judiciously with drugs will reduce the morbidity and mortality. VRE surveillance of family members of recently discharged patients VRE-infected, to limit the spread of infection.
A RETROSPECTIVE STUDY ON COEXISTENCE OF PULMONARY HYDATID CYST AND ASPERGILLOSIS
Anwesha Chatterjee, Goswami H.M., Dhotre S.V. Raval A.A.,Dharsandia M.V., Vegad M.M.
International Journal of Microbiology Research , 2000,
Abstract: Hydatid disease is a rare zoonotic disease. Rarely the hydatid cyst can become infected with mycotic organisms, such as Aspergillus. We describe a young male who presents with clinical features of suppurative lung abscess whose workup diagnosed hydatid cyst complicated by Aspergillus co-infection.A 32-year-old male farm labourer hospitalized with complaints of fever, chills, and productive cough with chest pain since four months duration Significant findings included leukocytosis with eosinophilia. Chestx-ray showing cystic lesion with air fluid level in Left lung suggestive of Hydatid cyst. CECT imaging of thorax and abdomen showed encysted left hydro pneumothoraxwith hypo dense cystic lesion in right lobe of liver,suggesting possibility of hydatid. The patient underwent decortication of the pulmonary cyst. Histopathologicalexamination of surgical specimen revealed cyst of 10 cm in its largest diameter showing cyst wall with thin branching septate hyphae with spores along with pink laminated wall like structure suggestive of hydatid cyst with aspergilloma. Aspergillosis and Hydatid cyst coexistance should be considered in presence of pulmonary cavitary lesion particularly in endemic areas. Detection of such association is important for planning adequate management.
EFFICACY OF FINE NEEDLE ASPIRATION CYTOLOGY, ZIEHL-NEELSEN (Z-N) STAIN AND CULTURE (BACTEC) IN DIAGNOSIS OF TUBERCULOSIS LYMPHADENITIS
Goswami H.M., Parikh U.R., Barot H.P.,Vaghela G.M.,Yadav K.S., Gazali Z.A.,Vegad M.M.
International Journal of Microbiology Research , 2012,
Abstract: Background- Tuberculous Lymphadenitis is the commonest form of extra pulmonary tuberculosis and tissue diagnosis is the main stay in the diagnosis of extra-pulmonary tuberculosis. This study was conducted to compare cytology, ZN staining and culture findings of clinically suspected tuberculous lymphadenitis cases. Methods- In the Present Study, clinically suspected cases of lymphadenopathy were undergone fine needle aspiration. The aspirates were examined cytologically followed by ZN staining and BACTEC culture. Results- The cytology suggestive of tuberculous lymphadenitis was found in 46 (76.6%) cases out of total 61 cases. Ziehl Neelsen stain demonstrated positive for acid fast bacilli (AFB) in 14 (22.9%) cases and mycobacteria isolated from BACTEC in36 (59%) cases. Out of 61 cases 15(24.5%) cases showed pus formation and in cytological examination 13 of those cases reported as tuberculous lymphadenitis. Aspiration mixed with blood demonstrated AFB positive in 2 (5.88%) and mycobacteria were isolated in 16 (48.48%) cases and cytology suggest tuberculous lymphadenitis in 21 (63.63%) cases. Seven out of Nine cases of necrosis with or without neutrophils show presence of AFB. Samples having epithelioid cell granuloma with or without necrosis show AFB positive in 5 (16.66%) and 2 (25%) cases and mycobacteria isolated in 22 (73.73%) and 5 (62.5%) cases respectively. Conclusion- In spite of the diagnostic pitfalls, the outcomes of this study reinforce the importance of opinion i.e. Fine Needle Aspiration Cytology serves as a potent and accurate diagnostic tool forpatients presenting with Lymphadenopathy due to tuberculosis.
MICROBIOLOGICAL SURVEILLANCE OF INTENSIVE CARE UNITS IN A TERTIARY CARE TEACHING HOSPITAL-WESTERN INDIA
Raval P.N., Patel P.G., Patel B.V., Soni S.T., Bhatt S.K., Vegad M.M., Goswami H.M.,Vaghela G.M.,Yadav K.S., Shirish Patil
International Journal of Microbiology Research , 2012,
Abstract: Introduction- Infection is a commonly encountered problem for patients in intensive care units (ICUs) and Multidrug-resistant bacterial infection is predominant. In intensive care unit (ICU) isolation, identification and antibiotic susceptibility pattern of microbiological isolate is important because of increased rate of mortality and morbidity and their economic burden which constitutes serious problem around the globe. Materials and Methods- This study was conducted over a period of one year in the different ICUs of a tertiary care, teaching Hospital in Western India. Different clinical samples were collected and analyzed by routine conventional methods in a Microbiology laboratory. Antibiotic susceptibility (ABS) test was done using modified Kirby-Bauer disk diffusion method as per CLSI guidelines. Results- Total 1145 clinical samples were processed, out of which 554 showed evidence of infection, 469 were gram-negative, 64 were gram-positive and 21 were fungal infection specially candida species. Urinary tract infections and Skin and soft tissue infections were most common infection in MICU/PICU and SICU respectively. Among the isolates, the most commonly found microorganism was Pseudomonas spp. in skin lesions, soft tissue wounds & in blood. However E. coli was predominant in urinary tract infection and Klebsiella pneumoniae in respiratory tract infection. The majority of the Gram-negative isolates were resistant to multiple antibiotics while 59.46% of Staphylococcus aureus isolates were Methicillin Resistant Staphylococcus aureus (MRSA).Conclusion- Outcomes of this study is highlighting the significance of microbial isolation & identification in patients in Intensive care Units (ICU) and shall improve antibiotic choices for number of patients admitted each year. Regular surveillance of antibiotic susceptibility pattern is important clue to clinicians in choosing antibiotics for the patients in ICU.
Eosinophil-active chemokines: assessment of in vivo activity
Teixeira, M.M.;
Brazilian Journal of Medical and Biological Research , 1998, DOI: 10.1590/S0100-879X1998000100003
Abstract: the selective recruitment of eosinophils in tissue is a striking feature of allergic diseases. recently, a family of chemoattractant molecules, namely chemokines, has been described which potently activates eosinophil function in vitro. we have developed a murine model of eosinophil recruitment to compare the relative potency and efficacy of chemokines in vivo. of the chemokines tested, only eotaxin and mip-1a induced significant accumulation of eosinophils in vivo, but eotaxin was more effective than mip-1a. chemokines, especially eotaxin acting via the ccr-3 receptor, may have a fundamental role in determining selective eosinophil recruitment in vivo
Study of ion beam sputtering using a glow discharge ion source
Abdelrahman, M.M.;
Brazilian Journal of Physics , 2010, DOI: 10.1590/S0103-97332010000100005
Abstract: in this work, sputtering yield in a glow discharge ion source system has been determined using the operating parameters of the ion source. the sputtering yield is found to be varied between 0.4 to 1 atoms removed per incident ion for nitrogen while for argon between 0.2 to 1.3 atoms removed per incident ion. the feature of this ion source is high output ion beam current and small size. operation of the ion source is quite simple since a stable discharge can be obtained within a large range of main parameters such as, discharge voltage, discharge current and gas pressure. also, beam profile for argon ion beam produced from the glow discharge ion source at id = 2,3 ma (discharge current) using argon gas with different gas pressures has been investigated.
Torsional vibration of carbon nanotubes under initial compression stress
Selim, M.M.;
Brazilian Journal of Physics , 2010, DOI: 10.1590/S0103-97332010000300004
Abstract: this study examines torsional vibration of single-walled carbon nanotubes (swcnts) subjected to initial compression stresses. the nanotube structures are treated within the multilayer thin shell approximation with the elastic properties taken to be those of the graphene sheet. simplified flügge shell equations of motion are proposed as the governing equations of vibration for the carbon nanotubes. a new equation of motion and phase velocity of torsional waves propagating in carbon nanotubes (cnts) subjected to initial compression stresses have been derived. the study reveals that the initial stresses present in the tube has a notable effect on the propagation of torsional waves. the results has been discussed and shown graphically. this investigation is very significant for potential application and design of nanoelectronics and nanodevices.
Corrosion Behaviour of Zn-Al-Cu Alloy in HCl Solution and its Inhibition
Ahmed,M.M.;
Portugaliae Electrochimica Acta , 2006,
Abstract: the corrosion behaviour of zn - al - cu alloy was studied in pure hcl solutions and in the presence of different concentrations of inhibitors. the techniques of measurements were: weight loss, linear polarization, polarization impedance and corrosion penetration. it was found that the corrosion rate of this alloy is greatly higher than that of zn or al. the inhibitors used were: heterocyclic pyridine (p), 2-methyl pyridine (2 mp), 3-methyl pyridine (3 mp) and 4-methyl pyridine (4 mp). these inhibitors behave as mixed type and their inhibition efficiency increases according to the order: 2 mp < 3 mp < p <4 mp. the values of activation energy of corrosion were determined in pure acid and in the presence of inhibitors. it was found that the presence of these inhibitors increases the value of activation within the same order of their inhibitory effect. the adsorption of the investigated inhibitors on the surface of the alloy follows frumkin?s isotherm.
Comparative Histological Study Vagus Nerve in Different Parts of Body on the Rabbits
M.M. Salech
Journal of Animal and Veterinary Advances , 2012,
Abstract: Through studying of vagus nerves in rabbits, histological compound observed that contain myelin nerve fiber but can be found variation percentage of different region of body, and non-myelin nerve fibers with different shapes of nucleus of the schwann cell depended on the area of nerve and function of the vagus nerve.
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