oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

4 ( 1 )

2020 ( 10 )

2019 ( 132 )

2018 ( 202 )

Custom range...

Search Results: 1 - 10 of 77194 matches for " Vanessa Eid da Silva;Buzalaf "
All listed articles are free for downloading (OA Articles)
Page 1 /77194
Display every page Item
Fluoride uptake in dental enamel after using fluoridated dentifrice, preceded or not by a CaCl2 solution rinse
úbeda, Liliane Torsani;Cardoso, Vanessa Eid da Silva;Buzalaf, Marília Afonso Rabelo;
Journal of Applied Oral Science , 2005, DOI: 10.1590/S1678-77572005000400019
Abstract: introduction: the use of a calcium solution previously to brushing could favor the remineralization and the fluoride (f) uptake in dental enamel. objective: this study evaluated the f in dental enamel after using a f dentifrice, preceded or not by a rinse with a cacl2 solution. methods: ten subjects (18-30 years) participated in a double-blind, cross-over protocol. enamel biopsy and 3 min saliva samples were obtained at baseline and after brushing (1.5 g crest?, 1,000 ppm f as naf), preceded by a rinse with 10 ml of 20 mm cacl2 50 mm acetate ph 7.2 or deionized water, for 1 min, followed by a 15 ml water rinse for 5 sec. biopsies were made before the rinses and after 8-15 and 120 min. saliva samples were collected before the rinses and after 4, 15, 30, 60 and 120 min. f concentration in enamel biopsies was analyzed with the f electrode (orion 9409) and a miniature calomel reference electrode, while phosphorus concentration was analyzed by spectrophotometry. saliva samples were analyzed for f with the electrode (orion 9609) by the direct method. the data were analyzed by anova and tukey test (p<0.05). results: mean f concentration ± sd (μg/ml) at baseline up to 120 min for saliva samples were: 0.02±0.01; 9.06±4.26; 2.01±1.76; 0.47±0.33; 0.24±0.17; 0.08±0.08; 0.07±0.04 for the deionized water rinse, and 0.02±0.02; 10.96±14.21; 2.76±3.04; 0.87±1.29; 0.40±0.66; 0.16±0.23; 0.09±0.09 for the cacl2 rinse. the data for enamel biopsies were (μg/g): 1861.7±1011.0; 1790.5±953.2; 1117.6±585.2 for the deionized water rinse, and 1586.4±776.8; 1536.3±546.4; 1520.5±1340.7 for the cacl2 rinse. conclusion: according to this protocol, there was not a significant variation in enamel f uptake after using a f dentifrice preceded or not by a rinse with a cacl2 solution.
Fluorine content of several brands of chocolate bars and chocolate cookies found in Brazil
Buzalaf Marília Afonso Rabelo,Granjeiro José Mauro,Cardoso Vanessa Eid da Silva,Silva Thelma Lopes da
Pesquisa Odontológica Brasileira , 2003,
Abstract: Chocolate bars and chocolate cookies are foodstuffs highly appreciated by children. The possibility of having fluorine (F) among their components, associated with an excessive consumption, may make them decisive contributors to the total daily F intake. Thus, they could participate in the establishment of dental fluorosis. The aim of this study was to analyze the fluorine concentration [F] of the chocolates bars (CB) Baton, Confeti, Garoto Ball, Kinder Ovo, M&M?s, Milkybar, Nescau, Nescau Ball, Surpresa, Surpresa Bichos, Tortuguita; and of the chocolate cookies (CC) Danyt?s, Hipopó, Nescau, Passatempo, Pokémon, Sítio do Pica-Pau Amarelo and Trakinas. Samples were purchased in Bauru, S o Paulo, Brazil. Three grams of each product were previously ashed at 525°C (CB and cookies fillings) and at 550°C (cookies dough), during 4 hours. Fluorine was separated from the ash by hexamethyldisiloxane (HMDS)-facilitated diffusion. Fluorine analysis was carried out with the specific electrode. Mean [F]s ± SD and amplitude (unit mg/g) were: CB = 0.30 ± 0.45 (0.07 - 1.60, n = 12) and CC = 1.08 ± 2.64 (0.04 - 7.10, n = 7). It was concluded that some of the analyzed foods may be important contributors to the total daily F intake. As for the product that had the highest [F] (Danyt?s), when only 3 units are consumed just once a day, they may supply up to 40% of the maximum recommended daily F intake (0.07 mg/kg body weight) for a 2-year-old child (12 kg). The [F] in these products should be informed on their labels.
Fluoride content of several breakfast cereals and snacks found in Brazil
Cardoso, Vanessa Eid da Silva;Olympio, Kelly Polido Kaneshiro;Granjeiro, José Mauro;Buzalaf, Marília Afonso Rabelo;
Journal of Applied Oral Science , 2003, DOI: 10.1590/S1678-77572003000400006
Abstract: breakfast cereals and snacks are foodstuffs highly appreciated by children, and the possibility that they contain substantial amounts of fluoride, associated with their widespread consume, may make them important contributors to the total daily fluoride intake. the aim of this study was to analyze the fluoride content on several breakfast cereals (a) and snacks (b) purchased in brazil. the analysis were made after hmds-facilitated diffusion (taves) using the ion-specific electrode (9609). mean fluoride concentrations ± sd (range, unit mg f/g) were: a= 0.76 ± 0.60 (0.08-1.86, n=15) and b= 0.32 ± 0.09 (0.22-0.55, n=18). our results suggest that the total amount of fluoride available in some products may contribute to the total daily fluoride intake. the product labels should provide information on their fluoride content to prevent fluorosis at the age of risk.
Fluorine content of several brands of chocolate bars and chocolate cookies found in Brazil
Buzalaf, Marília Afonso Rabelo;Granjeiro, José Mauro;Cardoso, Vanessa Eid da Silva;Silva, Thelma Lopes da;Olympio, Kelly Polido Kaneshiro;
Pesquisa Odontológica Brasileira , 2003, DOI: 10.1590/S1517-74912003000300005
Abstract: chocolate bars and chocolate cookies are foodstuffs highly appreciated by children. the possibility of having fluorine (f) among their components, associated with an excessive consumption, may make them decisive contributors to the total daily f intake. thus, they could participate in the establishment of dental fluorosis. the aim of this study was to analyze the fluorine concentration [f] of the chocolates bars (cb) baton, confeti, garoto ball, kinder ovo, m&m?s, milkybar, nescau, nescau ball, surpresa, surpresa bichos, tortuguita; and of the chocolate cookies (cc) danyt?s, hipopó, nescau, passatempo, pokémon, sítio do pica-pau amarelo and trakinas. samples were purchased in bauru, s?o paulo, brazil. three grams of each product were previously ashed at 525°c (cb and cookies fillings) and at 550°c (cookies dough), during 4 hours. fluorine was separated from the ash by hexamethyldisiloxane (hmds)-facilitated diffusion. fluorine analysis was carried out with the specific electrode. mean [f]s ± sd and amplitude (unit mg/g) were: cb = 0.30 ± 0.45 (0.07 - 1.60, n = 12) and cc = 1.08 ± 2.64 (0.04 - 7.10, n = 7). it was concluded that some of the analyzed foods may be important contributors to the total daily f intake. as for the product that had the highest [f] (danyt?s), when only 3 units are consumed just once a day, they may supply up to 40% of the maximum recommended daily f intake (0.07 mg/kg body weight) for a 2-year-old child (12 kg). the [f] in these products should be informed on their labels.
Nail and bone surface as indicators of acute exposure to fluoride in rats
Buzalaf, Marília Afonso Rabelo;Linardi, Myrna Maria;Carvalho, Juliane Guimar?es de;Cardoso, Vanessa Eid da Silva;Cury, Jaime Aparecido;
Journal of Applied Oral Science , 2004, DOI: 10.1590/S1678-77572004000400006
Abstract: this study evaluated the use of nails and bone surfaces as indicators of acute exposure to fluoride in rats. six groups (n=10/grp), aged 70 days, received, by gastrogavage, single doses of sodium fluoride containing 10, 35, 45, 60, 75 and 90 mg fluoride/kg body weight. the control group received deionized water. two hours after fluoride administration, the rats were killed and their plasma, nails (halves near to the growth end) and femur were collected. nail and plasma fluoride concentrations were analyzed with the electrode following hmds-facilitated diffusion. femur surface fluoride was removed from a circular area (4.52 mm2) by immersion in 0.5m hcl for 15 seconds, buffered with tisab and analyzed with the electrode. data were analyzed by anova and tukey's test (p<0.05) and by linear regression (p<0.001). average fluoride concentrations ranged from 55.4 to 91.5 mg/g for nails; from 0.019 to 6.937 mg/ml for plasma and from 617 to 2,394 mg/g for femur surface. fluoride concentrations in nails from experimental groups were not different from control. regarding plasma fluoride, all experimental groups differed from control, except the group that received the lowest dose. in respect to femur surface fluoride, only the group that received the highest dose differed from control. a strong correlation was found between plasma fluoride and the dose administered (r=0.736) and a medium correlation was found between femur surface and the dose administered (r=0.510). data suggest that two hours after an acute fluoride administration, nails and femur surface are not good indicators of fluoride exposure.
Urinary fluoride output in children following the use of a dual-fluoride varnish formulation
Olympio, Kelly Polido Kaneshiro;Cardoso, Vanessa Eid da Silva;Bijella, Maria Fernanda Borro;Pessan, Juliano Pelim;Delbem, Alberto Carlos Botazzo;Buzalaf, Marília Afonso Rabelo;
Journal of Applied Oral Science , 2009, DOI: 10.1590/S1678-77572009000300009
Abstract: objective: this study evaluated the bioavailability of fluoride after topical application of a dual-fluoride varnish commercially available in brazil, when compared to duraphattm. material and methods: the urinary fluoride output was evaluated in seven 5-year-old children after application of the fluoride varnishes, in two different phases. in the first phase (i), children received topical application of the fluoride varnish duofluorid xii (2.92% fluorine, calcium fluoride + 2.71% fluorine, sodium fluoride, fgmtm). after 1-month interval (phase ii), the same amount (0.2 ml) of the fluoride varnish duraphat (2.26% fluorine, sodium fluoride, colgatetm) was applied. before each application all the volunteers brushed their teeth with placebo dentifrice for 7 days. urinary collections were carried out 24 h prior up to 48 h after the applications. fluoride intake from the diet was also estimated. fluoride concentration in diet samples and urine was analyzed with the fluoride ion-specific electrode and a miniature calomel reference electrode coupled to a potentiometer. data were tested by anova and tukey's post hoc test (p<0.05). results: there were significant differences in the urinary fluoride output between phases i and ii. the use of duofluorid xii did not significantly increase the urinary fluoride output, when compared to baseline levels. the application of duraphat caused a transitory increase in the urinary fluoride output, returning to baseline levels 48 h after its use. conclusions: the tested varnish formulation, which has been shown to be effective in in vitro studies, also can be considered safe.
Fluoridation of the public water supply and prevalence of dental fluorosis in a peripheral district of the municipality of Bauru, SP
Ramires, Irene;Olympio, Kelly Polido Kaneshiro;Maria, Andréa Gutierrez;Pessan, Juliano Pelim;Cardoso, Vanessa Eid Silva;Lodi, Carolina Simonetti;Buzalaf, Marília Afonso Rabelo;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000200013
Abstract: objectives: the objectives of this study were to assess the fluoride concentration in the public water supply and the prevalence of dental fluorosis in schoolchildren between 7 and 15 years old, living in a peripheral district of the municipality of bauru. material and methods: for this, fifty two water samples were collected on three different days of one week. these samples were analyzed for fluoride by means of the ion-sensitive electrode method (orion 9609) coupled to a potentiometer (procyon, model 720). in this method, 1.0 ml of tisab ii (orion) was added to 1.0 ml of the sample. for the epidemiological survey of fluorosis, 52 schoolchildren of both genders, aged between 7 and 15 were assessed, with prior authorization from their caretakers. only one person examined the children, after supervised toothbrushing and drying with cotton wool rolls. the tf index was used. results: the fluoride concentrations in the water samples ranged from 0.62 to 1.20 mg/l, with a mean of 0.9 mg/l. the prevalence of dental fluorosis was 33%, with severity ranging from tf1 to tf4 (kappa of 0.73 and concordance of 83.33%). conclusions: the results from the analysis of water samples indicated a fluoride concentration greater than recommended for bauru. the fluorosis levels found were higher than expected for a peripheral district, in which water is one of the few sources of fluoride.
Evaluation of the total fluoride intake of 4-7-year-old children from diet and dentifrice
Pessan, Juliano Pelim;Silva, Salete Moura Bonifácio da;Buzalaf, Marília Afonso Rabelo;
Journal of Applied Oral Science , 2003, DOI: 10.1590/S1678-77572003000200012
Abstract: this study measured the total daily fluoride (f) intake of 21 children, living in bauru, a fluoridated community. f intake from diet (d) and dentifrice (b) was determined for 2 groups of children: a (4-5-year-old) and b (6-7-year-old). the method of 24-hour duplicate diet was used. f ingested from dentifrice was indirectly obtained, subtracting the amount expelled and the amount left on the toothbrush from the amount initially loaded onto the brush. f analysis was carried out with the ion specific electrode (orion 9409), after hexamethyldisiloxane-facilitated diffusion. the amount of f intake from d and b was divided by the child weight and total intake (t) was calculated by adding d and b. data were analyzed by student?s "t" (p<0.05) test and by linear regression. results (mean ± sd, mg f/kg body weigh) of t, d and b were, respectively: 0.056±0.040; 0.018±0.12; 0.037±0.038 for all children; 0.055±0.026; 0.021±0.014; 0.034±0.027 for group a and 0.057±0.052; 0.016±0.010; 0.041±0.046 for group b. there was a strong positive correlation between the amount of dentifrice used and the amount of fluoride ingested (r = 0.92, p<0.0001). one-third of the children analyzed were exposed to a f dose above 0.07 mg / kg body weight, which is recommended as the upper limit of daily f intake. dentifrice was the main source of f ingested by children with percentages of 57.43 ± 29.02 for all children; 56.49 ± 31.82 for group a; and 58.29 ± 27.78 for group b. no significant differences were found between the 2 groups.
Optimization of the Cultivation of Donor Mesenchymal Stromal Cells for Clinical Use in Cellular Therapy  [PDF]
Vanessa Valim, Bruna Amorin, Annelise Pezzi, Maria Aparecida Lima da Silva, Ana Paula Alegretti, Lucia Silla
CellBio (CellBio) , 2014, DOI: 10.4236/cellbio.2014.31003
Abstract: Mesenchymal stromal cells(MSC)have shown their benefits in graft-versus-host disease(GVHD), with five unsettled matters: 1) MSCs expansion in medium with Fetal Bovine Serum(FBS)and its risk of xenoreaction; 2) The number of cells indicated for therapy that is relatively high, with the need to optimize the expansion, number and time wise; 3) The utilization of third party donors; 4) Culture passage number(P);and 5) Source of the cells. This study was designed to determine the superiority of the Platelet Lysates(PL)over FBS on the expansion of MSC, the optimal cell plating density and days between each pass, and to investigate if donor total nucleated cells(TNC)obtained from the washouts of discharged bags and filters of hematopoietic stem cell transplantation(HSCT)can be expanded to be used at clinical grade. TNC were removed, plated and after the first passage were cultivated in different concentrations with FBS or PL, and the number of days to reach 80% of confluence was observed. Next, cultures with the same plating density were fed either with PL or with FBS and after seven days counted to analyze how much they had grown in that period. The proliferation of mesenchymal stromal cells in the presence of PL and SFB was averaged 11.88 and 2.5 times, respectively, in a period of 7 days. The highest concentration of plating cells using PL took less time to reach confluence as compared with the three lower ones. This study suggests that the PL is the best choice as a supplement to expand MSC and to allow the proliferation of enough number of MSC at P2 for clinical use.
Temperature Gradient Gel Electrophoresis as a Valuable Accessory Tool for Assessment of Dysbiosis in Crohn’s Disease  [PDF]
Vanessa Rafaela de Carvalho, Rogeria Keller, Ana Carolina da Silva Santos, Josias Rodrigues
Advances in Microbiology (AiM) , 2016, DOI: 10.4236/aim.2016.68055
Abstract: Escherichia coli and other Proteobacteria are augmented and several other bacteria are diminished in Crohn’s (CD) disease patients’ intestine. This imbalance in bacterial species composition—termed dysbiosis—seems to be determinant of CD manifestation. Since a great part of intestinal bacteria are not cultivable, detection of CD dysbiosis is accomplished by molecular tools, involving sequences analysis of the 16SrRNA gene (16SrDNA) present in the patient’s clinical samples, which can be done by sequencing or electrophoresis in denaturing gels of 16SrDNA amplicons. By analyzing, by temperature gradient gel electrophoresis (TGGE) and next generation sequencing of 16SV6-V8rDNA amplicons present in gram negative cultures from four distinct clinical samples of a control subject and a CD patient, this study demonstrates that both techniques were able to detect E. coli overgrowth and reduction in species richness in CD and that TGGE can discriminate sequences collectively labeled as “unclassified” in 16SrDNA databases. Although TGGE per se does not identify the sequences, the discriminatory power that it confers represents valuable accessory information to next generation DNA sequencing (NGS), and as such must be used as a NGS complementary tool.
Page 1 /77194
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.