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Search Results: 1 - 10 of 130426 matches for " V.;Wassem "
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Evidence for the endophytic colonization of Phaseolus vulgaris(common bean) roots by the diazotroph Herbaspirillum seropedicae
Schmidt, M.A.;Souza, E.M.;Baura, V.;Wassem, R.;Yates, M.G.;Pedrosa, F.O.;Monteiro, R.A.;
Brazilian Journal of Medical and Biological Research , 2011, DOI: 10.1590/S0100-879X2011007500004
Abstract: herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. in the present study, we have investigated the attachment to and internal colonization of phaseolus vulgaris roots by the h. seropedicae wild-type strain smr1 and by a strain of h. seropedicae expressing a red fluorescent protein (dsred) to track the bacterium in the plant tissues. two-day-old p. vulgaris roots were incubated at 30°c for 15 min with 6 x 108 cfu/ml h. seropedicae smr1 or ram4. three days after inoculation, 4 x 104 cells of endophytic h. seropedicae smr1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 106 cfu/g. the identity of the recovered bacteria was confirmed by amplification and sequencing of the 16srrna gene. furthermore, confocal microscopy of p. vulgaris roots inoculated with h. seropedicae ram4 showed that the bacterial cells were attached to the root surface 15 min after inoculation; fluorescent bacteria were visible in the internal tissues after 24 h and were found in the central cylinder after 72 h, showing that h. seropedicae ram4 is capable of colonizing the roots of the dicotyledon p. vulgaris. determination of dry weight of common bean inoculated with h. seropedicae smr1 suggested that this bacterium has a negative effect on the growth of p. vulgaris.
Early colonization pattern of maize (Zea mays L. Poales, Poaceae) roots by Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
Monteiro, Rose A.;Schmidt, Maria A.;Baura, Valter A. de;Balsanelli, Eduardo;Wassem, Roseli;Yates, Marshall G.;Randi, Marco A.F.;Pedrosa, Fábio O.;Souza, Emanuel M. de;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008005000007
Abstract: the bacterium herbaspirillum seropedicae is an endophytic diazotroph found in several plants, including economically important poaceous species. however, the mechanisms involved in the interaction between h. seropedicae and these plants are not completely characterized. we investigated the attachment of herbaspirillum to maize roots and the invasion of the roots by this bacterium using h. seropedicae strain smr1 transformed with the suicide plasmid putkandsred, which carries a mini-tn5 transposon containing the gene for the discosoma red fluorescent protein (dsred) constitutively expressed together with the kanamycin resistance gene. integration of the mini-tn5 into the bacterial chromosome yielded the mutant h. seropedicae strain ram4 which was capable of expressing dsred and could be observed on and inside fresh maize root samples. confocal microscopy of maize roots inoculated with h. seropedicae three days after germination showed that h. seropedicae cell were attached to the root surface 30 min after inoculation, were visible in the internal tissues after twenty-four hours and in the endodermis, the central cylinder and xylem after three days.
The type III secretion system is necessary for the development of a pathogenic and endophytic interaction between Herbaspirillum rubrisubalbicans and Poaceae
Maria A Schmidt, Eduardo Balsanelli, Helisson Faoro, Leonardo M Cruz, Roseli Wassem, Valter A de Baura, Vinicius Weiss, Marshall G Yates, Humberto M F Madeira, Lilian Pereira-Ferrari, Maria H P Fungaro, Francine M de Paula, Luiz F P Pereira, Luiz G E Vieira, Fábio L Olivares, Fábio O Pedrosa, Emanuel M de Souza, Rose A Monteiro
BMC Microbiology , 2012, DOI: 10.1186/1471-2180-12-98
Abstract: A 21?kb DNA region of the H. rubrisubalbicans genome contains a cluster of 26 hrp/hrc genes encoding for the type three secretion system (T3SS) proteins. To investigate the contribution of T3SS to the plant-bacterial interaction process we generated mutant strains of H. rubrisubalbicans M1 carrying a Tn5 insertion in both the hrcN and hrpE genes. H. rubrisulbalbicans hrpE and hrcN mutant strains of the T3SS system failed to cause the mottled stripe disease in the sugarcane susceptible variety B-4362. These mutant strains also did not produce lesions on Vigna unguiculata leaves. Oryza sativa and Zea mays colonization experiments showed that mutations in hrpE and hrcN genes reduced the capacity of H. rubrisulbalbicans to colonize these plants, suggesting that hrpE and hrcN genes are involved in the endophytic colonization.Our results indicate that the T3SS of H. rubrisubalbicans is necessary for the development of the mottled stripe disease and endophytic colonization of rice.
Nonsmooth Optimization Algorithms in Some Problems of Fracture Dynamics  [PDF]
V. V. Zozulya
Intelligent Information Management (IIM) , 2010, DOI: 10.4236/iim.2010.211073
Abstract: Mathematical statement of elastodynamic contact problem for cracked body with considering unilateral restrictions and friction of the crack faces is done in classical and weak forms. Different variational formulations of unilateral contact problems with friction based on boundary variational principle are considered. Nonsmooth optimization algorithms of Udzawa’s type for solution of unilateral contact problem with friction have been developed. Convergence of the proposed algorithms has been studied numerically.
Precessing Ball Solitons as Self-Organizing Systems during a Phase Transition in a Ferromagnet  [PDF]
V. V. Nietz
Applied Mathematics (AM) , 2013, DOI: 10.4236/am.2013.410A3010

Precessing ball solitons (PBS) in a ferromagnet during the first order phase transition is induced by a magnetic field directed along the axis of anisotropy, while the action of the periodic field perpendicular to the main magnetic field has been analyzed. Under these conditions, the characteristics of arising equilibrium PBS are uniquely determined by the frequency of the periodic field, but the solitons with other frequencies are impossible. For such structure, the entropy increase connected with dissipation is compensated by the decrease of the entropy due to the external periodic field. It is shown that the equilibrium PBS are essentially the “self-organizing systems” that can arise spotaneously in a metastable state of ferromagnet.

Movement of Self-Organizing Solitons in Ferromagnet  [PDF]
V. V. Nietz
Applied Mathematics (AM) , 2015, DOI: 10.4236/am.2015.610155
Abstract: Precessing ball solitons (PBS) in a ferromagnet during the first order phase transition induced by a magnetic field directed along the axis of anisotropy, while the additional action of high-frequency field perpendicular to the main magnetic field, are analyzed. It is shown that the spatial motion of solitons, associated with thermal fluctuations in the crystal, does not destroy the equilibrium of self-organized PBS.
Lα Line of Dark Positronium as a Nongravitational Detection of DM  [PDF]
V. Burdyuzha, V. Charugin
Journal of Modern Physics (JMP) , 2015, DOI: 10.4236/jmp.2015.613187
Abstract: An attempt to predict the new atomic dark matter lines is done on the example of a dark lepton atom-positronium. Its Layman-alpha line with the energy near 3 GeV may be observable if the appropriate conditions are realized. For this we have studied a γ-ray excess in the center of our galaxy. In principle, this excess may be produced by the Lα line of a dark positronium in the medium with Compton scattering. The possibility of observations of an annihilation line (E~300 TeV) of dark positronium is also predicted. Other proposals to observe the atomic dark matter are shortly described. Besides, Hα line (1.3μ) of usual positronum must be observable in the direction on the center of our galaxy.
Randomization of Energy and Momentum in Statistical Mechanics  [PDF]
V. V. Paranjape
Journal of Modern Physics (JMP) , 2015, DOI: 10.4236/jmp.2015.615223
Abstract: Particle-particle collisions in materials give rise to a particle distribution in energy and momentum in such a way that a most probable distribution is realized. I will show that an evaporating liquid generates a molecular imbalance in the equilibrium energy distribution. The molecular collisions by their inherent nature are able to repair the imbalance and in so doing it is shown that the liquids cool down. Similarly an external electric field creates substantial imbalance in the momentum distribution for the electrons in ionic semiconductors. Electron-electron collisions are able to restore the imbalance and as a consequence, similar to the evaporating liquid, the electron gas loses thermal energy and cools down.
基于税收能力视角的中国税制改革 China s Tax Reform Based on the Perspective of Tax Capacity
- , 2015,
Abstract: 摘要 税收能力是研究税收制度的重要视角,科学判定税收能力是税制改革的基本前提。税收制度决定了既定条件下税收能力的高低,税收能力也影响和制约着税制改革的进程。我国近年来的经济社会发展改变了经济运行中各经济主体的税收能力及其格局。基于税收能力视角,现行税收制度在税制结构、税制体系、征管制度等方面还存在着与经济社会发展不相适应之处。为此,税制改革应基于对目前及未来税收能力的科学判断,明确税制结构优化的方向和路径,加快地方税体系建设,进一步完善税收征管制度。
Lorentz’s Transformations and Gravitation in the Granular Space Theory  [PDF]
V Konushko
Journal of Modern Physics (JMP) , 2011, DOI: 10.4236/jmp.2011.26053
Abstract: Research into the read structure of space at ways leads to the conclusion on the existence of a privileged (absolute) system of reference, with all the equations remaining invariant about Lorentz’s transformations. The expansion of these transformations makes it possible to obtain easily the Schwarzshild matrix and, also, all the results of Einstein’s theory of gravity. The untangling of the physical meaning of velocity as a measure of relative deformation of elementary space cells eliminates, at last, all the paradoxes of Lorentz’s transformations and allows visual observation of the mechanism of gravity and Coulomb interaction in imaginary experiments.
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