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Search Results: 1 - 10 of 1952 matches for " Upendra Nath Dwivedi "
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Genetic Diversity of Pigeonpea (Cajanus cajan (L.) Millsp.) Cultivars and Its Wild Relatives Using Randomly Amplified Polymorphic DNA (RAPD) Markers  [PDF]
Kusum Yadav, Sanjay Kumar Yadav, Anurag Yadav, Veda Prakash Pandey, Upendra Nath Dwivedi
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.33038
Abstract: Genetic diversity among and between 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its wild relatives (C. albicans and C. lineatus) analysed using RAPD. Twenty two random primers with an average of 71.2% polymorphism produced 151 polymorphic bands. Cluster analysis based on these 151 RAPD markers revealed relatively low level (0.434 - 0.714) of genetic diversity among cultivars and high level of diversity between cultivars and wild relatives. C. albicans and C. lineatus showed only 0.231 similarity with each other and C. albicans showed relatively higher similarity with C. cajan cultivars than that showed by C. lineatus. In dendrogram the 16 cultivars grouped into two distinct clusters comprising of seven and nine genotypes each while the wild species form out groups. Bootstrap analysis of the dendrogram was performed and resulted in significant bootstrap values. Principal components analysis (PCA) also revealed the similar results that of unweighted pair group method with arithmetic mean (UPGMA). The first, second and third PCs contributed 55.9%, 5.9%, and 5.6% of the variation, respectively, with cumulative variation of the first three PCs was 67.4%.
Comparative Analysis of Genetic Diversity among Cultivated Pigeonpea (Cajanus cajan (L) Millsp.) and Its Wild Relatives (C. albicans and C. lineatus) Using Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) Fingerprinting  [PDF]
Kusum Yadav, Sanjay Kumar Yadav, Anurag Yadav, Veda Prakash Pandey, Upendra Nath Dwivedi
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.511181
Abstract:

Genetic relationships of 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its two wild relatives (C. albicans and C. lineatus) from different parts of the India were analysed using 22 random amplified polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs) primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with an average of 6.8 polymorphic band/primer. Cluster analysis based on these 151 RAPD markers revealed relatively low level (0.434 - 0.714) of genetic diversity among cultivars and high level of diversity between cultivars and wild relatives. Ten ISSR primers produced 100 bands across 16 cultivars and its wild relatives out of which 93 (93%) were polymorphic with an average of 9.3 polymorphic band/primer. Cluster analysis based on these 93 ISSR markers also revealed relatively higher level (0.328 - 0.827) of genetic diversity among cultivars as compared to RAPD markers. The polymorphic markers obtained by both RAPD and ISSR primers were pooled and the genetic diversity analysis based on these 244 markers was analysed. Jaccard’s similarity coefficient obtained by pooled data revealed very narrow range (0.477 - 0.720) among cultivated and high range between cultivated and wild species C. albicans (0.240 - 0.331) and C. lineatus (0.163 - 0.193). In the UPGMA based dendrogram the 16 cultivars were grouped into three distinct clusters. Cluster I contained two genotypes, cluster II had six and cluster III had eight genotypes. Principal components analysis (PCA) also resulted in similar pattern as that of UPGMA based analysis. The first three PCs contributed 56.26%, 5.71% and 4.97% of variation, respectively, with cumulative variation of the first three PCs was 66.96%. Both the markers and the combined data revealed similar pattern with narrow diversity among cultivars and higher diversity between cultivars and wild one, but

Role of Efflux Pumps and Intracellular Thiols in Natural Antimony Resistant Isolates of Leishmania donovani
Smita Rai, Bhaskar, Sudhir K. Goel, Upendra Nath Dwivedi, Shyam Sundar, Neena Goyal
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074862
Abstract: Background In view of the recent upsurge in the phenomenon of therapeutic failure, drug resistance in Leishmania, developed under natural field conditions, has become a great concern yet little understood. Accordingly, the study of determinants of antimony resistance is urgently warranted. Efflux transporters have been reported in Leishmania but their role in clinical resistance is still unknown. The present study was designed to elucidate the mechanism of natural antimony resistance in L. donovani field isolates by analyzing the functionality of efflux pump(s) and expression profiles of known genes involved in transport and thiol based redox metabolism Methodology/Principal Findings We selected 7 clinical isolates (2 sensitive and 5 resistant) in addition to laboratory sensitive reference and SbIII resistant mutant strains for the present study. Functional characterization using flow cytometry identified efflux pumps that transported substrates of both P-gp and MRPA and were inhibited by the calmodulin antagonist trifluoperazine. For the first time, verapamil sensitive efflux pumps for rhodamine 123 were observed in L. donovani that were differentially active in resistant isolates. RT-PCR confirmed the over-expression of MRPA in isolates with high resistance index only. Resistant isolates also exhibited consistent down regulation of AQP1 and elevated intracellular thiol levels which were accompanied with increased expression of ODC and TR genes. Interestingly, γ-GCS is not implicated in clinical resistance in L. donovani isolates. Conclusions/Significance Here we demonstrate for the first time, the role of P-gp type plasma membrane efflux transporter(s) in antimony resistance in L. donovani field isolates. Further, decreased levels of AQP1 and elevated thiols levels have emerged as biomarkers for clinical resistance.
Marketing of Sabai Grass in Socio-Economic Development of Tribals in Mayurbhanj District, Orissa (India)
Priti Ranjan Hathy,Upendra Nath Sahu
International Journal of Business and Management , 2010, DOI: 10.5539/ijbm.v5n2p149
Abstract: Socio-Economic development involves an increase in the material well being of the society. In a country like India, about 80 per cent of the population living in rural area and around 70 per cent of the population dependent on agriculture. Mayurbhanj is one of the richest districts in Orissa so far as forest and mineral wealth are concerned. Sabai grass industry plays a predominant role in shaping the economic destiny of the rural people in the district. The objective of this paper is to analyse the innovative schemes and the role of Sabai grass industry for the economic developments of growers of the district; Results show that the tribal of Mayurbhanj district generates the Sabai grass product marketing demand in national and international market so as to develop their economic status.
Evaluative Measures of Search Engines
Jitendra Nath Singh,Dr. S.K. Dwivedi
International Journal of Electronics and Computer Science Engineering , 2012,
Abstract: The ability to search and retrieve information from the web efficiently and effectively is great challenge of search engine. Information retrieval on the Web is very different from retrieval in traditional indexed databases because it’s hyper-linked character, the heterogeneity of document types and authoring styles. Thus, since Web retrieval is substantially different from information retrieval, new or revised evaluative measures are required to assess retrieval performance using search engines. In this paper we suggested a number of evaluative measures to evaluate the effectiveness of search engines. The motivation behind each of these measures is presented, along with their descriptions and definitions.
2D-QSAR model development and analysis on variant groups of anti -tuberculosis drugs
Neeraja Dwivedi*,Bhartendu Nath Mishra,Vishwa Mohan Katoch
Bioinformation , 2011,
Abstract: A quantitative structure activity relationship study was performed on different groups of anti-tuberculosis drug compound for establishing quantitative relationship between biological activity and their physicochemical /structural properties. In recent years, a large number of herbal drugs are promoted in treatment of tuberculosis especially due to the emergence of MDR (multi drug resistance) and XDR (extensive drug resistance) tuberculosis. Multidrug-resistant TB (MDR-TB) is resistant to front-line drugs (isoniazid and rifampicin, the most powerful anti-TB drugs) and extensively drug-resistant TB (XDR-TB) is resistant to front-line and second-line drugs. The possibility of drug resistance TB increases when patient does not take prescribed drugs for defined time period. Natural products (secondary metabolites) isolated from the variety of sources including terrestrial and marine plants and animals, and microorganisms, have been recognized as having antituberculosis action and have recently been tested preclinically for their growth inhibitory activity towards Mycobacterium tuberculosis or related organisms. A quantitative structure activity relationship (QSAR) studies were performed to explore the antituberculosis compound from the derivatives of natural products . Theoretical results are in accord with the in vitro experimental data with reported growth inhibitory activity towards Mycobacterium tuberculosis or related organisms. Antitubercular activity was predicted through QSAR model, developed by forward feed multiple linear regression method with leave-one-out approach. Relationship correlating measure of QSAR model was 74% (R2 = 0.74) and predictive accuracy was 72% (RCV2 = 0.72). QSAR studies indicate that dipole energy and heat of formation correlate well with anti-tubercular activity. These results could offer useful references for understanding mechanisms and directing the molecular design of new lead compounds with improved anti-tubercular activity. The generated QSAR model revealed the importance of structural, thermodynamic and electro topological parameters. The quantitative structure activity relationship provides important structural insight in designing of potent antitubercular agent.
Policy into practice: an experience of Higher Education Link in Child and Adolescent Psychiatry
Kedar Nath Dwivedi,Nisha Dogra,Indira Sharma,Karen Bretherton
Journal of Indian Association for Child and Adolescent Mental Health , 2005,
Abstract: Aim The main aim of the Child and Adolescent Overseas Working Party has been to support the development of services in low-income countries through enhancing their training capacity. This is congruent with the British Council s policy of Higher Education Link. The paper shares an experience of translating such a policy into practice. Method The experience of implementing a British Council Higher Education Link in Child and Adolescent Psychiatry between the two Universities in Varanasi and Leicester is shared. Implication In spite of various difficulties, all parties concerned learnt some valuable lessons and found it to be a worthwhile venture.
SCANNING ELECTRON MICROSCOPY AS AN ANALYTICAL TOOL FOR PARTICLE SIZE DISTRIBUTION AND ASPECT RATIO ANALYSIS OF CIPROFLOXACIN MUCOADHESIVE POLYMERIC SUSPENSION
Subhashree Sahoo, Chandra Kanti Chakraborti, Subash Chandra Mishra & Upendra Nath Nanda
International Journal of Research and Reviews in Applied Sciences , 2011,
Abstract:
Comparison of Net Global Warming Potential and Greenhouse Gas Intensity Affected by Management Practices in Two Dryland Cropping Sites  [PDF]
Upendra M. Sainju
Journal of Environmental Protection (JEP) , 2015, DOI: 10.4236/jep.2015.69092
Abstract: Little is known about the effect of management practices on net global warming potential (GWP) and greenhouse gas intensity (GHGI) that account for all sources and sinks of greenhouse gas (GHG) emissions in dryland cropping systems. The objective of this study was to compare the effect of a combination of tillage, cropping system, and N fertilization on GWP and GHGI under dryland cropping systems with various soil and climatic conditions from 2008 to 2011 in western North Dakota and eastern Montana, USA. Treatments in western North Dakota with sandy loam soil and 373 mm annual precipitation were conventional till malt barley (Hordeum vulgarie L.) with 67 kg N ha-1 (CTB/N1), conventional till malt barley with 0 kg N ha-1 (CTB/N0), no-till malt barley-pea (Pisum sativum L.) with 67 kg N ha-1 (NTB-P/N1), no-till malt barley with 67 kg N ha-1 (NTB/N1), and no-till malt barley with 0 kg N ha-1 (NTB/N0). In eastern Montana with loam soil and 350 mm annual precipitation, treatments were conventional till malt barley-fallow with 80 kg N ha-1 (CTB-F/N1), conventional till malt barley-fallow with 0 kg N ha-1 (CTB-F/N0), no-till malt barley-pea with 80 kg N ha-1 (NTB-P/N1), no-till malt barley with 80 kg N ha-1 (NTB/N1), and no-till malt barley with 0 kg N ha-1 (NTB/N0). Carbon dioxide sink as soil C sequestration rate at the 0 - 10 cm depth was greater in NTB-P/N1 and NTB/N1 than the other treatments at both sites and greater in eastern Montana than western North Dakota. Carbon dioxide sources were greater with N fertilization than without and greater with conventional till than no-till. Soil total annual N2O and CH4 fluxes varied among treatments, years, and locations. Net GWP and GHGI were lower in NTB-P/N1 than the other treatments in western North Dakota and lower in NTB-P/N1 and NTB/N1 than the other treatments in eastern Montana. Net GWP across similar treatments was lower in eastern Montana than western North Dakota, but GHGI was similar. Annualized crop yield was greater in the treatments with N fertilization than without. Because of greater grain yield but lower GWP and GHGI, no-till malt barley-pea rotation with adequate N fertilization can be used as a robust management practice to mitigate net GHG emissions while sustaining dryland crop yields, regardless of soil and climatic conditions. Loam soil reduced GWP and crop yields compared with sandy loam soil.
Simulation Analysis on Applicability of Meta Material and PBG Based mm-Wave Planar Antenna for Advanced Cellular Technologies  [PDF]
Smrity Dwivedi
Open Journal of Antennas and Propagation (OJAPr) , 2017, DOI: 10.4236/ojapr.2017.51003
Abstract: Metamaterials have got a unique identification in the field of electromagnetic. Left handed metamaterials have been recognized through its working at high frequencies with larger bandwidth in antenna design. The author has proposed a multi substrates LH metamaterials with an array of split triangular antennas for wideband as well as for larger bandwidth. FR4 is being used at frequency 42 - 26 GHz with thickness of 3 mm for substrate and matching circuit is inserted to get high gain with minimum reflections. The number of rods in PBG is 100, which is found to get high return losses and ideal behavior of VSWR. Obtained results have been in good agreement such as S11 is approximately 30 dB, VSWR 1.8 dB and the bandwidth enhancement is 2 GHz at frequency 5 GHz. All results are plotted using commercial software CST microwave studio (Version 2012) and MATLAB.
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