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Search Results: 1 - 10 of 49411 matches for " Tsung-Jung Wu "
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Transcriptional activation of the Axl and PDGFR-α by c-Met through a ras- and Src-independent mechanism in human bladder cancer
Chen-Yun Yeh, Shin-Mei Shin, Hsuan-Heng Yeh, Tsung-Jung Wu, Jyh-Wei Shin, Tsuey-Yu Chang, Giri Raghavaraju, Chung-Ta Lee, Jung-Hsien Chiang, Vincent S Tseng, Yuan-Chii G Lee, Cheng-Huang Shen, Nan-Haw Chow, Hsiao-Sheng Liu
BMC Cancer , 2011, DOI: 10.1186/1471-2407-11-139
Abstract: Both NIH-Met5 and T24-Met3 cell lines harboring an inducible human c-Met gene were established. C-Met-related RTKs were screened by RTK microarray analysis. The cross-talk of RTKs was demonstrated by Western blotting and confirmed by small interfering RNA (siRNA) silencing, followed by elucidation of the underlying mechanism. The impact of this cross-talk on biological function was demonstrated by Trans-well migration assay. Finally, the potential clinical importance was examined in a cohort of 65 cases of locally advanced and metastatic bladder cancer patients.A positive association of Axl or platelet-derived growth factor receptor-alpha (PDGFR-α) with c-Met expression was demonstrated at translational level, and confirmed by specific siRNA knock-down. The transactivation of c-Met on Axl or PDGFR-α in vitro was through a ras- and Src-independent activation of mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK/ERK) pathway. In human bladder cancer, co-expression of these RTKs was associated with poor patient survival (p < 0.05), and overexpression of c-Met/Axl/PDGFR-α or c-Met alone showed the most significant correlation with poor survival (p < 0.01).In addition to c-Met, the cross-talk with Axl and/or PDGFR-α also contributes to the progression of human bladder cancer. Evaluation of Axl and PDGFR-α expression status may identify a subset of c-Met-positive bladder cancer patients who may require co-targeting therapy.The RTK c-Met is expressed during normal development and plays a crucial role in many cell regulatory processes [1]. After binding to its cognate ligand-hepatocyte growth factor (HGF), activated c-Met transmits signals implicated in the cell proliferation, motility, survival, and morphogenesis [2-4]. C-Met is over-expressed and usually associated with metastatic progression of a variety of human malignant tumors, including bladder cancer [1,5]. We have reported that c-Met is over-expressed in 32.3%, 63.2%, and 65.2% of superfici
Effects of Combinatorial Treatment with Pituitary Adenylate Cyclase Activating Peptide and Human Mesenchymal Stem Cells on Spinal Cord Tissue Repair
Kuan-Min Fang,Jen-Kun Chen,Shih-Chieh Hung,Mei-Chun Chen,Yi-Ting Wu,Tsung-Jung Wu,Hsin-I Lin,Chia-Hua Chen,Henrich Cheng,Chung-Shi Yang,Shun-Fen Tzeng
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0015299
Abstract: The aim of this study is to understand if human mesenchymal stem cells (hMSCs) and neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) have synergistic protective effect that promotes functional recovery in rats with severe spinal cord injury (SCI). To evaluate the effect of delayed combinatorial therapy of PACAP and hMSCs on spinal cord tissue repair, we used the immortalized hMSCs that retain their potential of neuronal differentiation under the stimulation of neurogenic factors and possess the properties for the production of several growth factors beneficial for neural cell survival. The results indicated that delayed treatment with PACAP and hMSCs at day 7 post SCI increased the remaining neuronal fibers in the injured spinal cord, leading to better locomotor functional recovery in SCI rats when compared to treatment only with PACAP or hMSCs. Western blotting also showed that the levels of antioxidant enzymes, Mn-superoxide dismutase (MnSOD) and peroxiredoxin-1/6 (Prx-1 and Prx-6), were increased at the lesion center 1 week after the delayed treatment with the combinatorial therapy when compared to that observed in the vehicle-treated control. Furthermore, in vitro studies showed that co-culture with hMSCs in the presence of PACAP not only increased a subpopulation of microglia expressing galectin-3, but also enhanced the ability of astrocytes to uptake extracellular glutamate. In summary, our in vivo and in vitro studies reveal that delayed transplantation of hMSCs combined with PACAP provides trophic molecules to promote neuronal cell survival, which also foster beneficial microenvironment for endogenous glia to increase their neuroprotective effect on the repair of injured spinal cord tissue.
Cadmium Induces Apoptosis in Pancreatic β-Cells through a Mitochondria-Dependent Pathway: The Role of Oxidative Stress-Mediated c-Jun N-Terminal Kinase Activation
Kai-Chih Chang, Ching-Cheng Hsu, Shing-Hwa Liu, Chin-Chuan Su, Cheng-Chieh Yen, Ming-Jye Lee, Kuo-Liang Chen, Tsung-Jung Ho, Dong-Zong Hung, Chin-Ching Wu, Tien-Hui Lu, Yi-Chang Su, Ya-Wen Chen, Chun-Fa Huang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0054374
Abstract: Cadmium (Cd), one of well-known highly toxic environmental and industrial pollutants, causes a number of adverse health effects and diseases in humans. The growing epidemiological studies have suggested a possible link between Cd exposure and diabetes mellitus (DM). However, the toxicological effects and underlying mechanisms of Cd-induced pancreatic β-cell injury are still unknown. In this study, we found that Cd significantly decreased cell viability, and increased sub-G1 hypodiploid cells and annexin V-Cy3 binding in pancreatic β-cell-derived RIN-m5F cells. Cd also increased intracellular reactive oxygen species (ROS) generation and malondialdehyde (MDA) production and induced mitochondrial dysfunction (the loss of mitochondrial membrane potential (MMP) and the increase of cytosolic cytochrome c release), the decreased Bcl-2 expression, increased p53 expression, poly (ADP-ribose) polymerase (PARP) cleavage, and caspase cascades, which accompanied with intracellular Cd accumulation. Pretreatment with the antioxidant N-acetylcysteine (NAC) effectively reversed these Cd-induced events. Furthermore, exposure to Cd induced the phosphorylations of c-jun N-terminal kinases (JNK), extracellular signal-regulated kinases (ERK)1/2, and p38-mitogen-activated protein kinase (MAPK), which was prevented by NAC. Additionally, the specific JNK inhibitor SP600125 or JNK-specific small interference RNA (si-RNA) transfection suppressed Cd-induced β-cell apoptosis and related signals, but not ERK1/2 and p38-MAPK inhibitors (PD98059 and SB203580) did not. However, the JNK inhibitor or JNK-specific si-RNA did not suppress ROS generation in Cd-treated cells. These results indicate that Cd induces pancreatic β-cell death via an oxidative stress downstream-mediated JNK activation-triggered mitochondria-regulated apoptotic pathway.
Acute and Prolonged Adverse Effects of Temperature on Mortality from Cardiovascular Diseases
Yu-Kai Lin, Chin-Kuo Chang, Yu-Chun Wang, Tsung-Jung Ho
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0082678
Abstract: Background Cardiovascular diseases are the leading causes of death worldwide, especially for developed countries. Elevated mortality from cardiovascular diseases has been shown related to extreme temperature. We thus assessed the risk of mortality from cerebrovascular diseases, heart diseases, and ischemic heart disease (IHD) in relation to temperature profiles in four subtropical metropolitans (Taipei, Taichung, Tainan, and Kaohsiung) from 1994 to 2007 in Taiwan. Methods Distributed lag non-linear models were applied to estimate the cumulative relative risks (RRs) with confidence intervals of cause-specific mortality associated with daily temperature from lag 0 to 20 days, and specific effect of extreme temperature episodes with PM10, NOx, and O3, and other potential confounders controlled. Estimates for cause-specific mortalities were then pooled by random-effect meta-analysis. Results Comparing to centered temperature at 27°C, the cumulative 4-day (lag 0 to 3) risk of mortality was significantly elevated at 31°C for cerebrovascular diseases (RR = 1.14; 95% CI: 1.00, 1.31) and heart diseases (RR = 1.22; 95% CI: 1.02, 1.46) , but not for IHD (RR = 1.09; 95% CI: 0.99, 1.21). To the other extreme, at 15°C, the cumulative 21-day (lag 0 to 20) risk of mortality were also remarkably increased for cerebrovascular diseases, heart diseases, and IHD (RRs = 1.48 with 95% CI: 1.04, 2.12, 2.04 with 95% CI: 1.61, 2.58, and 1.62 with 95% CI: 1.30, 2.01, respectively). Mortality risks for cardiovascular diseases were generally highest on the present day (lag 0) of extreme heat. No particular finding was detected on prolonged extreme temperature event by pooling estimations for cause-specific mortality. Conclusions Low temperature was associated with greater risk of mortality from cardiovascular diseases in comparison with that of high temperature. Adverse effects of extreme temperatures are acute at the beginning of exposure.
Effect of Qigong on quality of life: a cross-sectional population-based comparison study in Taiwan
Tsung-Jung Ho, David C Christiani, Tso-Chiang Ma, Tsong-Rong Jang, Chih-Hui Lieng, Yi-Chun Yeh, Shinn-Zong Lin, Jaung-Geng Lin, Jim-Shoung Lai, Tzuo-Yun Lan
BMC Public Health , 2011, DOI: 10.1186/1471-2458-11-546
Abstract: A total of 165 individuals practicing Waitankung were matched by age and sex with 660 general individuals for comparison. Information about health-related quality of life, measured by the SF-36, and other basic and health conditions was obtained from the questionnaires. This study used the linear mixed-effect regression model to examine the association between health-related quality of life and the practice of Waitankung.Compared with either sedentary individuals or individuals practicing other types of exercise, the Waitankung group scored higher for eight and five out of ten SF-36 components, respectively. The Waitankung group scored better in general health, vitality, and physical component summary compared to individuals participating in other types of exercise, even when considering the energy expended by exercise.The results suggest that Waitankung exercising is significantly associated with health-related quality of life. Waitankung may serve as an exercise choice for middle-aged and older people to improve overall quality of life.Among the tools for promoting health, physical activity plays an essential role because of its beneficial effect on health, including reduced risk of mortality and developing cancer, type II diabetes, and high blood pressure in the general population, and geriatric conditions in the older population [1]. In Chinese society, Tai Chi Chuan and Qigong are two popular physical activities, mainly because of their potential physical and mental benefits and gentle movements suitable for middle-aged and old people. Several studies have found that Tai Chi Chuan and Qigong provide health benefits similar to other physical activities [2,3]. For example, in a recent review of randomized control trials for Tai Chi Chuan and Qigong, both provide similar health benefits of bone density, cardiopulmonary effects, physical function, falls, quality of life, self-efficacy, reported health outcomes, psychological symptoms, and immune function [3].In Tai
Metabolomic Dynamic Analysis of Hypoxia in MDA-MB-231 and the Comparison with Inferred Metabolites from Transcriptomics Data
I-Lin Tsai,Tien-Chueh Kuo,Tsung-Jung Ho,Yeu-Chern Harn,San-Yuan Wang,Wen-Mei Fu,Ching-Hua Kuo,Yufeng Jane Tseng
Cancers , 2013, DOI: 10.3390/cancers5020491
Abstract: Hypoxia affects the tumor microenvironment and is considered important to metastasis progression and therapy resistance. Thus far, the majority of global analyses of tumor hypoxia responses have been limited to just a single omics level. Combining multiple omics data can broaden our understanding of tumor hypoxia. Here, we investigate the temporal change of the metabolite composition with gene expression data from literature to provide a more comprehensive insight into the system level in response to hypoxia. Nuclear magnetic resonance spectroscopy was used to perform metabolomic profiling on the MDA-MB-231 breast cancer cell line under hypoxic conditions. Multivariate statistical analysis revealed that the metabolic difference between hypoxia and normoxia was similar over 24 h, but became distinct over 48 h. Time dependent microarray data from the same cell line in the literature displayed different gene expressions under hypoxic and normoxic conditions mostly at 12 h or earlier. The direct metabolomic profiles show a large overlap with theoretical metabolic profiles deduced from previous transcriptomic studies. Consistent pathways are glycolysis/gluconeogenesis, pyruvate, purine and arginine and proline metabolism. Ten metabolic pathways revealed by metabolomics were not covered by the downstream of the known transcriptomic profiles, suggesting new metabolic phenotypes. These results confirm previous transcriptomics understanding and expand the knowledge from existing models on correlation and co-regulation between transcriptomic and metabolomics profiles, which demonstrates the power of integrated omics analysis.
Clinical Analysis and Strategy for Liver Transplantation in Patients with Pre-existing Portal Vein Thrombosis
Tsung-Han Wu,Yann-Sheng Lin,Chen-Fang Lee,Ting-Jung Wu
Chang Gung Medical Journal , 2011,
Abstract: Background: Liver transplantation (LT) in patients with portal vein thrombosis (PVT)remains a challenge for transplant surgeons. In this study, we included agroup of patients with PVT who underwent LT, and analyzed patient outcomes.Methods: A total of 356 patients who underwent LT consisting of 167 cases ofdeceased donor LT and 189 cases of live donor LT at Chang Gung MemorialHospital Linkou Medical Center between September 1996 and June 2009were retrospectively reviewed; 24 (6.7%) of these patients had PVT at transplantation. Their clinical features, surgical management, and outcomes wereanalyzed.Results: Surgical management of patients with PVT included a thrombectomy followed by direct anastomosis between the recipient’s and the liver graft portalvein (PV) (n = 13), interposition vein graft between the recipient’s coronaryvein (CV) and the liver graft PV (n = 3), direct anastomosis of the recipient’sCV and the liver graft PV (n = 1), interposition jump graft from the recipient’s superior mesenteric vein to the liver graft PV (n = 4), and transection ofthe thrombotic PV followed by interposition of a venous graft between therecipient’s PV and the liver graft PV (n = 3). There were 7 hospital mortalities. The mean follow-up for the 17 surviving patients was 36.3 months(range, 3.4–105.1 months), and 14 patients were still alive at the end of thestudy. Four patients (16.7%) had rethrombosis of portal inflow after LT.Patients with PVT undergoing LT had a significantly higher mortality rate (p= 0.033) than patients without PVT undergoing LT. However, there was nosignificant difference in the cumulative survival rates (p = 0.0696). Furtheranalysis of patient survival according to PVT grade, venous graft application,and reconstructed portal flow routes also exhibited no significant differences.Conclusions: LT for patients with PVT is clinically feasible and should not be considered acontraindication. However, a favorable outcome is achievable only with idealsurgical management to overcome PVT during LT.
Parametrized Post-Newtonian Limit of Teleparallel Dark Energy Model
Jung-Tsung Li,Yi-Peng Wu,Chao-Qiang Geng
Physics , 2013, DOI: 10.1103/PhysRevD.89.044040
Abstract: We study the post-Newtonian limit in the teleparallel equivalent of General Relativity with a scalar field which non-minimally couples to gravity. The metric perturbation is obtained from the vierbein field expansion with respect to the Minkowski background. Due to the structure of the teleparallel gravity Lagrangian, the potential of the scalar field shows no effect to the parametrized post-Newtonian parameters, and compatible results with Solar System observations are found.
Psychometric Evaluation of the Internalized Stigma of Mental Illness Scale for Patients with Mental Illnesses: Measurement Invariance across Time
Chih-Cheng Chang, Tsung-Hsien Wu, Chih-Yin Chen, Jung-Der Wang, Chung-Ying Lin
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0098767
Abstract: Background The current investigation examined the psychometric properties of the Internalized Stigma of Mental Illness (ISMI) scale in a sample of patients with mental illness. In addition to the internal consistency, test-retest reliability, and concurrent validity that previous studies have tested for the ISMI, we extended the evaluation to its construct validity and measurement invariance using confirmatory factor analysis (CFA). Methods Three hundred forty-seven participants completed two questionnaires (i.e., the ISMI and the Depression and Somatic Symptoms Scale [DSSS]), and 162 filled out the ISMI again after 50.23±31.18 days. Results The results of this study confirmed the frame structure of the ISMI; however, the Stigma Resistance subscale in the ISMI seemed weak. In addition, internal consistency, test-retest reliability, and concurrent validity were all satisfactory for all subscales and the total score of the ISMI, except for Stigma Resistance (α = 0.66; ICC = 0.52, and r = 0.02 to 0.06 with DSSS). Therefore, we hypothesize that Stigma Resistance is a new concept rather than a concept in internalized stigma. The acceptable fit indices supported the measurement invariance of the ISMI across time, and suggested that people with mental illness interpret the ISMI items the same at different times. Conclusion The clinical implication of our finding is that clinicians, when they design interventions, may want to use the valid and reliable ISMI without the Stigma Resistance subscale to evaluate the internalized stigma of people with mental illness.
Superconductor made by electrolyzed and oxidized water
Chia-Jyi Liu,Tsung-Hsien Wu,Lin-Li Hsu,Jung-Sheng Wang,Shu-Yo Chen,Wei Jen Chang,Jiunn-Yuan Lin
Physics , 2006,
Abstract: By deintercalation of Na+ followed by inserting bilayers of water molecules into the host lattice, the layered cobalt oxide of gamma-Na0.7CoO2 undergoes a topotactic transformation to a layered cobalt oxyhydrate of Na0.35(H2O)1.3CoO2-delta with the c-axis expanded from c = 10.9 anstrom to c = 19.6 anstrom. In this paper, we demonstrate that the superconducting phase of c = 19.6 anstrom can be directly obtained by simply immersing gamma-Na0.7CoO2 powders in electrolyzed/oxidized (EO) water, which is readily available from a commercial electrolyzed water generator. We found that high oxidation-reduction potential of EO water drives the oxidation of the cobalt ions accompanying by the formation of the superconductive c = 19.6 anstrom phase. Our results demonstrate how EO water can be used to oxidize the cobalt ions and hence form superconducting cobalt oxyhydrates in a clean and simple way and may provide an economic and environment-friendly route to oxidize the transition metal of complex metal oxides
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