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Search Results: 1 - 10 of 79069 matches for " Tso-Hsiao Chen "
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Far-Infrared Therapy Induces the Nuclear Translocation of PLZF Which Inhibits VEGF-Induced Proliferation in Human Umbilical Vein Endothelial Cells
Yung-Ho Hsu, Yen-Cheng Chen, Tso-Hsiao Chen, Yuh-Mou Sue, Tzu-Hurng Cheng, Jia-Rung Chen, Cheng-Hsien Chen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0030674
Abstract: Many studies suggest that far-infrared (FIR) therapy can reduce the frequency of some vascular-related diseases. The non-thermal effect of FIR was recently found to play a role in the long-term protective effect on vascular function, but its molecular mechanism is still unknown. In the present study, we evaluated the biological effect of FIR on vascular endothelial growth factor (VEGF)-induced proliferation in human umbilical vein endothelial cells (HUVECs). We found that FIR ranging 3~10 μm significantly inhibited VEGF-induced proliferation in HUVECs. According to intensity and time course analyses, the inhibitory effect of FIR peaked at an effective intensity of 0.13 mW/cm2 at 30 min. On the other hand, a thermal effect did not inhibit VEGF-induced proliferation in HUVECs. FIR exposure also inhibited the VEGF-induced phosphorylation of extracellular signal-regulated kinases in HUVECs. FIR exposure further induced the phosphorylation of endothelial nitric oxide (NO) synthase (eNOS) and NO generation in VEGF-treated HUVECs. Both VEGF-induced NO and reactive oxygen species generation was involved in the inhibitory effect of FIR. Nitrotyrosine formation significantly increased in HUVECs treated with VEGF and FIR together. Inhibition of phosphoinositide 3-kinase (PI3K) by wortmannin abolished the FIR-induced phosphorylation of eNOS and Akt in HUVECs. FIR exposure upregulated the expression of PI3K p85 at the transcriptional level. We further found that FIR exposure induced the nuclear translocation of promyelocytic leukemia zinc finger protein (PLZF) in HUVECs. This induction was independent of a thermal effect. The small interfering RNA transfection of PLZF blocked FIR-increased PI3K levels and the inhibitory effect of FIR. These data suggest that FIR induces the nuclear translocation of PLZF which inhibits VEGF-induced proliferation in HUVECs.
Are Non-cardiac Surgeries Safe for Dialysis Patients? – A Population-Based Retrospective Cohort Study
Yih-Giun Cherng, Chien-Chang Liao, Tso-Hsiao Chen, Duan Xiao, Chih-Hsiung Wu, Ta-Liang Chen
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0058942
Abstract: Background End-stage renal disease represents a risk complex that complicates surgical results. The surgical outcomes of dialysis patients have been studied in specific fields, but the global features of postoperative adverse outcomes in dialysis patients receiving non-cardiac surgeries have not been examined. Methods Taiwan’s National Health Insurance Research Database was used to study 8,937 patients under regular dialysis with 8,937 propensity-score matched-pair controls receiving non-cardiac surgery between 2004 and 2007. We investigated the influence of hemodialysis and peritoneal dialysis, effects of hypertension and diabetes, and impact of additional comorbidities on postoperative adverse outcomes. Results Postoperative mortality in dialysis patients was higher than in controls (odds ratio [OR] 3.33, 95% confidence interval [CI] 2.56 to 4.33) when receiving non-cardiac surgeries. Complications such as acute myocardial infarction, pneumonia, bleeding, and septicemia were significantly increased. Postoperative mortality was significantly increased among peritoneal dialysis patients (OR 2.71, 95% CI 1.70 to 4.31) and hemodialysis patients (OR 3.42, 95% CI 2.62 to 4.47) than in controls. Dialysis patients with both hypertension and diabetes had the highest risk of postoperative complications; these risks increased with number of preoperative medical conditions. Patients under dialysis also showed significantly increased length of hospitalization, more ICU stays and higher medical expenditures. Conclusion Surgical patients under dialysis encountered significantly higher postoperative complications and mortality than controls when receiving non-cardiac surgeries. Different dialysis techniques, pre-existing hypertension/diabetes, and various comorbidities had complication-specific impacts on surgical adverse outcomes. These findings can help surgical teams provide better risk assessment and postoperative care for dialysis patients.
MicroRNA-328 Inhibits Renal Tubular Cell Epithelial-to-Mesenchymal Transition by Targeting the CD44 in Pressure-Induced Renal Fibrosis
Cheng-Hsien Chen, Chung-Yi Cheng, Yen-Cheng Chen, Yuh-Mou Sue, Chung-Te Liu, Tzu-Hurng Cheng, Yung-Ho Hsu, Tso-Hsiao Chen
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0099802
Abstract: Epithelial-mesenchymal transition (EMT) occurs in stressed tubular epithelial cells, contributing to renal fibrosis. Initial mechanisms promoting EMT are unknown. Pressure force is an important mechanism contributing to the induction and progression of renal fibrogenesis in ureteric obstruction. In our study of cultured rat renal tubular cells (NRK-52E) under 60 mmHg of pressure, we found that the epithelial marker E-cadherin decreased and mesenchymal markers, e.g., α-smooth muscle actin, fibronectin and Snail, increased. Pressure also induced the expression of connective tissue growth factor and transforming growth factor-β. MicroRNA array assays showed that pressure reduced miR-328 at the initial stage of pressurization. We identified a potential target sequence of miR-328 in rat CD44 3′-untranslated regions. In contrast with the miR-328 expression, CD44 expression was up-regulated at the initial pressurization stage. We also found that miR-328 expression decreased and CD44 increased in ureteric obstruction kidneys in the animal study. CD44 siRNA transfection significantly increased E-cadherin expression and inhibited pressure-induced EMT. Both hyaluronan binding peptide pep-1 and osteopontin neutralizing antibody inhibited pressure-induced EMT. Our results suggest that miR-328-mediated CD44 transient upregulation is an important trigger of the pressure-induced EMT in renal fibrosis.
Urotensin II Induces Interleukin 8 Expression in Human Umbilical Vein Endothelial Cells
Chung-Yi Lee, Yi-Tin Tsai, Shih-Hurng Loh, Ju-Chi Liu, Tso-Hsiao Chen, Hung-Hsing Chao, Tzu-Hurng Cheng, Jin-Jer Chen
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0090278
Abstract: Background Urotensin II (U-II), an 11-amino acid peptide, exerts a wide range of actions in cardiovascular systems. Interleukin-8 (IL-8) is secreted by endothelial cells, thereby enhancing endothelial cell survival, proliferation, and angiogenesis. However, the interrelationship between U-II and IL-8 as well as the detailed intracellular mechanism of U-II in vascular endothelial cells remain unclear. The aim of this study was to investigate the effect of U-II on IL-8 expression and to explore its intracellular mechanism in human umbilical vein endothelial cells. Methods/Principal Findings Primary human umbilical vein endothelial cells were used. Expression of IL-8 was determined by real-time quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and luciferase reporter assay. Western blot analyses and experiments with specific inhibitors were performed to reveal the downstream signaling pathways as concerned. U-II increased the mRNA/protein levels of IL-8 in human umbilical vein endothelial cells. The U-II effects were significantly inhibited by its receptor antagonist [Orn5]-URP. Western blot analyses and experiments with specific inhibitors indicated the involvement of phosphorylation of p38 mitogen-activated protein kinase and extracellular signal-regulated kinase in U-II-induced IL-8 expression. Luciferase reporter assay further revealed that U-II induces the transcriptional activity of IL-8. The site-directed mutagenesis indicated that the mutation of AP-1 and NF-kB binding sites reduced U-II-increased IL-8 promoter activities. Proliferation of human umbilical vein endothelial cells induced by U-II could be inhibited significantly by IL-8 RNA interference. Conclusion/Significance The results show that U-II induces IL-8 expression in human umbilical vein endothelial cells via p38 mitogen-activated protein kinase and extracellular signal-regulated kinase signaling pathways and IL-8 is involved in the U-II-induced proliferation of human umbilical vein endothelial cells.
Providing Frequency Support of Hydro-Pumped Storage to Taiwan Power System with Wind Power Integration  [PDF]
Yuan-Kang Wu, G. W. Chang, Bo-Yu Hsiao, Li-Tso Chang
Smart Grid and Renewable Energy (SGRE) , 2016, DOI: 10.4236/sgre.2016.74009
Abstract: The combination of wind and pumped storage is a useful method to compensate the fluctuation of wind power generation, which would exploit the abundant wind potential and increase wind power penetration. Taiwan Power Company (TPC) develops renewable energy actively in recent years. Moreover, TPC has started planning a high penetration wind power system and building offshore wind farms around the coast of Zhangbin, Yunlin and Penghu. The target of the offshore wind power installed capacity is up to 3 GW by 2025. However, the integration of the large scale of wind power would give huge challenges to the system operator because wind is randomly characterized. In this study, after high penetration wind power is integrated, the impacts of system frequency and the dispatch of conventional units will be discussed. Additionally, the hybrid system combing wind power with pumped-storage will be planning to reduce the effect of system frequency.
The Analyses of Purchasing Decisions and Brand Loyalty for Smartphone Consumers  [PDF]
Yu-Syuan Chen, Tso-Jen , Chen, Cheng-Che Lin
Open Journal of Social Sciences (JSS) , 2016, DOI: 10.4236/jss.2016.47018

With the advent of smartphone, customers purchasing smartphones are sharply increasing over years. Since the Smartphone market extend, the major brands are getting more competitive. Each brand manufacturers are dedicating in developing a strong operating system, software applications, fashionable appearance, service quality, and so on. The purpose of this study is to explore consumers’ purchasing behavior, determinants of purchasing decision, and factors of brand loyalty of smartphones. The findings stated that some external factors markedly influenced customers on choosing smartphones. For example, a customer bought a smartphone because of the demand for using it. On the contrary, few customers would buy a smartphone due to the pursuit of vogue or modern trend and the influences by other people. Furthermore, some internal elements of decision processes have sharply influence on choosing a smartphone. The majority of respondents agreed that the price, internal functions of a smartphone all played an important role on making a purchasing decision. In the opposite side of our original thinking, ninety-three respondents (37%) disagreed that they would choose the one with lower price between two smartphones. In the third part related to the brand relationship quality for purchasing a smartphone, most respondents could hurriedly recall the symbolic logo of the brand and the after-sale service would affect their willingness of rebuying the same brand. In contrast, even though the evaluation of this brand is not good, they still intend to continue to buy this brand. Finally, brand loyalty led consumers to happily tell others the advantages of this brand and recommend the purchase, and to rebuy the brand when it launches a new product. In conclusion, Customers’ perceived value, brand experience, trust, satisfaction, service quality and commitment are found to be the key influencing factors of brand loyalty.

Estimates of energy expenditure using the RT3 accelerometer in patients with systemic lupus erythematosus  [PDF]
Tim K. Tso, Wen-Nan Huang, Chen-Kang Chang
Health (Health) , 2010, DOI: 10.4236/health.2010.26089
Abstract: This study aimed to characterize energy expenditure patterns using the triaxial accelerometer and to identify the association of energy expenditure with clinical parameters in patients with systemic lupus erythematosus (SLE). Estimates of energy expenditures represented by total activity calorie (TA), physical activity calorie (PA), total activity calorie per body weight (TABW), and physical activity calorie per body weight (PABW) of 49 female SLE patients were assessed using the RT3 triaxial accelerometer (StayHealthy, Monrovia, CA) in a sevenday period. SLE patients in the highest body mass index (BMI) tertile showed significantly lower values of TABW compared to those in the lowest tertile, while SLE patients in the lowest TABW tertile showed significantly higher body weight, waist circumference, BMI, SLE disease activity index (SLEDAI), dosage of prednisone, and blood pressure. There was a high prevalence of metabolic syndrome and SLE patients with metabolic syndrome showed significantly lower TABW. In addition, both TABW and PABW significantly but negatively correlated with SLEDAI. In conclusion, the RT3 accelerometer is suitable for evaluating total and physical activity-related energy expenditure in patients with SLE. TABW measured by the triaxial accelerometer is inversely related with body weight status and disease activity in SLE patients. This suggests that estimates of energy expenditure by the tri-axial accelerometer may be applied in the management of SLE.
The association of circulating interleukin-18 with fasting insulin and weight loss in obese children  [PDF]
Tim K. Tso, Wen-Nan Huang, Chen-Kang Chang
Health (Health) , 2010, DOI: 10.4236/health.2010.27102
Abstract: Obesity is an independent risk factor for developing cardiovascular disease and increases insulin resistance in children. Interleukin (IL)-18 is a novel pro-inflammatory cytokine with potential atherogenetic properties. This study ai- med to identify circulating levels of IL-18 in obese children and examine the effects of combined nutritional education-physical activity course on circulating IL-18. Plasma IL-18, body mass index (BMI), fasting glucose and insulin, homeostasis model assessment insulin resistance (HOMA IR), lipid profile, uric acid, high- sensitive C-reactive protein (hs-CRP), and homocysteine were determined in 70 obese children aged 10-12 years before and after attending a 13-week weight reduction program, which included physical activities and nutritional education. Twenty-five age-matched non-obese children served as controls. At baseline, obese children had significantly higher levels of BMI, fasting insulin, HOMA IR, triglyceride (TG), uric acid, hs-CRP, and IL-18 but lower high-density lipoprotein-cholesterol (HDL-C) than non-obese children. Plasma IL-18 levels in obese children decreased significantly after the weight reduction program. At baseline, plasma IL-18 levels in obese children positively correlated with BMI, HOMA IR, insulin and TG but negatively correlated with HDL-C. There was a significant relationship between plasma IL-18 and BMI changes. Moreover, fasting insulin was responsible for IL-18 variability in obese children. These findings suggest that elevated plasma IL-18 levels in obese children are partly associated with parameters of obesity and insulin resistance, and are significantly affected by modest weight loss.
The Impact of Memory Load on Customer Choice Behavior  [PDF]
Hao-Te Lu, Tso-Jen Chen, Yi-Chou Wang
Open Journal of Social Sciences (JSS) , 2014, DOI: 10.4236/jss.2014.29041

There are only limited things that human brain can process at the same time, and people are adapted to save mental resources for things that are more important. Earlier research has shown that heuristics is employed to lighten brain load when people have to make quick decisions. However, it is not clear whether people will make rational choices if they have plenty of time. The purpose of this study is to investigate whether mental resources more or less will affect consumer behavior. In the experiment feeling/thinking (intuition vs. rational), working memory load (numbers memorization), will be employed to understand participants’ changing behaviors.

Impact of Transformational Leadership Behaviors and Psychological Optimism on Subordinate Performance in Taiwan’s Tourism Hotel Industry  [PDF]
Tso-Jen Chen, Chi-Min Wu, Yi-Chou Wang
Open Journal of Social Sciences (JSS) , 2015, DOI: 10.4236/jss.2015.37028

This study proposed a theoretical framework to explore the possible relationship among transformational leadership behavior, psychological optimism, and subordinate performance. Data were surveyed from 169 supervisor-subordinate dyads in Taiwan’s tourism hotel industry; we found that transformational leadership behaviors might help individuals nurture higher level of psychological optimism, thus enhancing the subordinate performance. Implication and suggestions were discussed.

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