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Probing molecular interaction between transferrin and anti-transferrin by atomic force microscope
Zhiwen Zheng,Peihui Yang,Gucheng Zeng,Jiye Cai
Chinese Science Bulletin , 2006, DOI: 10.1007/s11434-006-0405-0
Abstract: The interaction between transferrin (Tf) and its antibody was investigated by atomic force microscope. Tf-antibody was immobilized on the Au-coated glass slide, and the specific combination between antibody and antigen was also characterized by AFM. The results showed that holo-transferrin was jogged with anti-transferrin, and binded anti-transferrin more tightly than apo-transferrin. The force-distance curves revealed that the affinity of anti-transferrin and holo-transferrin was much stronger than that of apo-transferrin.
Simple and rapid detection of liver cirrhosis in children by tracking serum IgA/transferrin ratio
Ahmed M. Ibrahim,Mamdouh M. Ali,Hala M. Ghanem,Amriaa M. Mousa
Journal of Experimental and Integrative Medicine , 2011,
Abstract: Objective: The aim of this study is to assess the level of immunoglobulin A (IgA) and transferrin of children patients with liver cirrhosis to determine the relation between liver cirrhosis and IgA/transferrin ratio. Methods: The study involved 32 children classified into liver patients without cirrhosis (n=12), and liver patients with cirrhosis (n=10) as well as a group of normal healthy children (n=10) for comparison. In all of these subjects, serum alanine (ALT) and aspartate (AST) aminotransferase activity, as well as serum IgA and transferrin level, were also determined. Results: Our results revealed that the mean values of both ALT and AST activities were significantly high in both groups of patients with and without liver cirrhosis; the activity of both enzymes was relatively higher in patients with liver cirrhosis than in those without liver cirrhosis. Furthermore, the amount of IgA showed very significant decreased values in patients without liver cirrhosis while highly significant increased values were obtained in cirrhotic patients as compared to their corresponding values in normal group. The concentration of serum transferrin showed insignificant values in cases without liver cirrhosis, while it showed moderately significant decreased levels in cases of liver cirrhosis. Although the values of IgA/transferrin ratio showed insignificant values in patients without liver cirrhosis, these values were significantly high in cirrhotic patients. In addition, it has been found that in liver cirrhotic patients the mean values of IgA/transferrin ratio nearly reached 2.5 as compared to the ratios in normal or non-cirrhotic patients. Conclusions: From the present study we have postulated that the determination of IgA and transferrin in serum or plasma may open up a very simple and safe means for the early detection of latent cirrhosis. The value of IgA/transferrin >2.5 ratio was found to be a highly significant increase in latent cirrhosis as compared to children without cirrhosis or in normal children. This value can be considered as an indicator of latent cirrhosis in children. [J Exp Integr Med 2011; 1(2.000): 117-121]
Molecular Structure of Human Transferrin – Transferrin Receptor Complex
Viroj Wiwanitkit
International Journal of Molecular Sciences , 2006, DOI: 10.3390/i7070197
Abstract: Transferrin receptor (TfR) is a glycoprotein mediating the entry of ferrictransferrin (Tf) from the extracellular compartment into the cells. TfR are present on thesurface of many cell types but they are most abundant on cells active in hemoglobinsynthesis. However, the knowledge on the complex, recombination, between Tf and sTfR islimited. Here, the author performs an analysis to study the molecular structure of human Tf- sTfR complex. The output 3D molecular structure from the combination between Tf andTfR is derived. The property as well as geometry of the derived complex was also presented.
Prevalencia de hemocromatosis en trabajadores sanos: Importancia de a?adir en la analítica de perfil bioquímico una saturación de transferrina
Martínez-Vázquez,C.; Martínez Cadilla,J.; Gil,M.; Sope?a,B.; Torres,J.; Cordeiro,E.; Seijas,M.; Fuente,J. de la; Méndez,M. J.;
Anales de Medicina Interna , 2000, DOI: 10.4321/S0212-71992000001200002
Abstract: aim: hereditary hemochromatosis is the most common inherited disorder in white population (2-8 cases per 1000 habitants). hemochro -matosis is characterized by increased intestinal absortion of iron leading to its deposition into multiple organs. an early diagnosis and proper management with frecuent phlebotomies are known to improve life expentacy and quality of life. diagnosis is suggested by an elevated transferrin saturation (ts) (more than 60%). method: prospective study of the level of ts among 1131 healthy workers, who came to the security and hygiene official centre for their annual revision had been undertaken. results: twenty-wo workers had high ts; in 10 of them the increase of ts was confirmed on repeated determinations. liver biopsy was per -formed in six (and refused by the other four), eventualy a diagnosis of hemochromatosis was confirmed in three (in-group prevalence of 2.6 per 1000 people). conclusions: in onr experience, ts is the most appropiate initial screening test for detecting hereditary hemochromatosis in a normal population.
Carbohydrate-Deficient Transferrin - A Contemporary Biomarker in Comparison with Traditional Laboratory Markers of Chronic Alcohol Abuse
Neboj a Maksi , Tatjana Vodnik, Maja Stankovi , Sr an Milovanovi , Ljubi a Radivojevi , Ivana Obradovi , Marijana Dajak, Nada Majki -Singh
Journal of Medical Biochemistry , 2010, DOI: 10.2478/v10011-010-0011-1
Abstract: Timely identification of excessive alcohol use and its potential complications is a prerequisite for successful treatment. Several routine tests have been used in laboratories that may help in diagnosing alcoholism, such as determination of MCV, AST, ALT, GGT, but it has been shown that they lack specificity and sensitivity. Contemporary bio-markers are increasingly being used today that may due to their unique characteristics help in discovering the onset of chronic alcohol abuse, as well as in abstinence and relapse monitoring. The term carbohydrate-deficient transferrin (CDT) stands for a small group of human transferrin isoforms (asialo, monosialo, and disialotran sferrin) with a lower degree of glycosylation in comparison to the dominant transferrin isoform (tetrasialotransferrin). Persons consuming large quantities of alcohol (≥50-80 g daily) over a period of at least two weeks have increased concentrations of transferrin isoforms lacking one (disialotransferrin) or both (asialotransferrin) carbohydrate chains. In this paper the traditional markers of chronic alcohol abuse (GGT, AST, ALT, and MCV) were determined, as well as the new biomarker CDT, after which diagnostic evaluation was performed and their usability and clinical value in routine laboratory practice were estimated. These markers were also determined in heavy alcoholics on admission into hospital and after two weeks of therapy, with the aim of estimating their diagnostic value for abstinence and relapse monitoring.
"A New Protocol for Isolation and Purification of Transferrin from Human Serum"
Arefanian1,M Djalali
Iranian Journal of Public Health , 2002,
Abstract: Transferrin acts as a vehicle for iron transportation between the absorption, storage and utilization sites in the body and, besides iron, its two highaffinity iron-binding site are capable of binding to 29 other elements. In physiological and pathophysiological conditions, transferrin’s properties are different and close attention to these properties is required. For further investigation for the above properties, it is necessary to have this protein in highly pure state.The purpose of this research was to isolate and purify the human transferrin from serum with native properties and high purity. The results of this research lead to a protocol for the isolation and purification of human transferrin which is cost-effective and time saving to produce antitransferrin antibody for immunological kits.
The binding properties of some novel ruthenium (III) complexes with human serum transferrin
Arsene A. L.,,Uivarosi V.,Mitrea N.,Dragoi C. M.
Biopolymers and Cell , 2011,
Abstract: Aim. The transferrin cycle gained increased interest in recent years and it holds promise as an attractive system for strategies of drug targeting to tumors. Neoplasic cells exhibit a large demand of iron and therefore express highly transferrin receptors. As a consequence, transferrin conjugates can preferentially interact with cancer cells. This strategy is exploited nowadays for targeting novel anti-cancer drugs. Recent data showed that ruthenium (III) compounds possess antitumor and antimetastatic effects, due to their affinity for crucial biomolecules (like transferrin). Methods. The paper presents the transferrin-binding properties of some novel ruthenium (III) complexes with general formula RuL2(DMSO) mCl3·nH2O ((Ru-nf) L: norfloxacin (nf), m = 1, n = 1; (Ru-cpx) L: ciprofloxacin (cpx), m = 2, n = 2; (Ru-oflo) L: ofloxacin (oflo), m = 1, n = 1; (Ru-levo) L: levofloxacin (Levo), m = 2, n = 8; (Ru-pip) L: pipemidic acid (pip), m = 1, n = 2, DMSO: dimethylsulfoxide). We investigated, in vitro, the interactions of these ligands with human transferrin through spectroscopic techniques, with the ultimate goal of preparing adducts with good selectivity for cancer cells. Results. All studied complexes interact with human serum transferrin; the molar ratio [complex]/[transferrin] strongly influences the binding affinity. Conclusions. The best interaction between the complexes studied and transferrin is achieved for a molar ratio of 8; the best interaction was registered for Ru-pip, followed by Ru-nf.
Iron metabolism
Ana Maria de Souza
Einstein (S?o Paulo) , 2005,
Abstract: The present paper deals with iron metabolism, from its absorption,regulatory factors, storage, and distribution to body compartmentsaccording to existing knowledge.
Mutations in HFE and TFR2 genes in a Spanish patient with hemochromatosis
del-Castillo-Rueda,Alejandro; Cuadrado-Grande,Nuria; álvarez-Fernández,Emilio; Enríquez-de-Salamanca,Rafael; álvarez-Sala,Luis Antonio; Morán-Jiménez,María Josefa;
Revista Espa?ola de Enfermedades Digestivas , 2011, DOI: 10.4321/S1130-01082011000700010
Abstract: iron overload disease has a wide variety of genotypes. the genetic study of this disease confirms its hereditary nature and enables us to provide genetic counseling for first-degree relatives. we performed magnetic resonance imaging and liver biopsy in an asymptomatic patient with more than 1,000 μg/l of serum ferritin and studied the genes involved in this condition. the phenotype of iron overload is confirmed by a predominantly periportal pattern of iron deposits in the liver suggestive of genetic disease. in the case we present the molecular study revealed a double heterozygosity for the mutations c.187c>g (p.h63d) and c.840c>g (p.f280l) in the hfe and transferrin receptor 2 (tfr2) genes, respectively.
Parenteral Albumin Therapy in Burn Patients: A Randomized Controlled Trial
Ali Akbar Mohammadi,Mohammad Jalal Hashemi-Nasab,Reza Ershadi,Ahmad Reza Tavakkolian
Iranian Journal of Medical Sciences , 2010,
Abstract: Background: Administration of albumin for burn patients andits effects on mortality and morbidity has been debated foralong time. The aim of this study was to evaluate the effects ofalbumin administration on wound healing, length of hospitalstay, weight change, and mortality of burn patients.Methods: Two matched groups of patients from Shiraz BurnCare Center were randomly selected. The patients in onegroup received parenteral albumin in addition to high proteindiet and the other group received only high protein diet. Thelength of hospital stay, healing time, mortality, serum albumin,transferrin levels, and weight loss were compared.Results: A total of 141 patients were selected; 71 patients incontrol group and 70 patients in albumin group. There were nosignificant differences in mortality (P=0.97), length of hospitalstay (P=0.45), and healing time (P=0.25) in two groups. Thepatients who survived had significantly higher serum transferrinlevel (128 mg/dL versus 102 mg/dL).Conclusion: Parenteral albumin administration did not have asignificant effect on mortality, length of hospital stay, andhealing time in burn patients.Trial Registration Number: IRCT138802141605N3Iran J Med Sci 2010; 35(2): 95-100.
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