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Search Results: 1 - 10 of 73300 matches for " Trícia Osório da;Gioso "
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Vitrifica??o de ovócitos desnudados ou n?o e previamente maturados in vitro
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Silva, Trícia Osório da;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000500004
Abstract: this study aimed at the evaluation of the effects from cryopreservation of bovine oocytes in vitro matured, by using ethylene glycol (eg) associated to trehalose and polyvinylpyrrolidone (pvp), of ovary oocytes of slaughtered cows, randomly assigned to three treatments. treatment 0 (t0 - control): oocytes that were desnuded and not vitrified. treatment 1 (t1): cryopreservation of in vitro matured oocytes with cumulus oophorus. tratamento 2 (t2): cryopreservation of in vitro matured desnuded oocytes. the percentage of recovered oocytes after cryopreservation and with normal morphology was different for vitrified oocytes (94.7 and 76.8%; 69.5 and 48.85% for t1 and t2, respectively). the main changes ultrastructural in vitrificated oocytes were prematurely released of cortical granules. later, all normal oocytes were fecundated and cultivated at 38.5oc in atmosphere with 5% co2 for seven days. the fecundation and cleavage rates for treatments were different (56.2, 41.7 and 12.5%; 36.3, 0.0 and 0.0%, for t0, t1 and t2, respectively). morulas and blastocysts were obtained only in t0 (34.5%). these results indicate that, the used protocols, for vitrification procedure is not indicated for cryopreservation of matured bovine oocytes.
Les?o do músculo obturador externo em atletas de futebol profissional
Valente, Henrique Gon?alves;Marques, Felipe Osório;Souza, Luciano Da Silva De;Abib, Roberto Trápaga;Ribeiro, Daniel Cury;
Revista Brasileira de Medicina do Esporte , 2011, DOI: 10.1590/S1517-86922011000100007
Abstract: several studies have investigated different injuries that occur among soccer players. however, it has been noticed that none has mentioned injury to the external obturator muscle. as a small and monoarticulated muscle, its incidence is probably low and barely documented in the literature. therefore, the aim of this study is to present four external obturator muscle strain cases in a professional soccer team in 2008. the data was collected from a database using the software sistema de prepara??o desportiva which provides data such as name, diagnosis, trauma mechanism, clinical history, time away from training and progress. all athletes who undertook magnetic resonance imaging (mri) of the external obturator muscle and presented rupture of the external obturator muscle were included in the study. four external obturator muscle strain injuries were identified from a total of twenty-eight muscle strains during the year of 2006. all athletes presented diffuse pain on the hip for medial and lateral rotation of the joint. the predominant injury mechanism was lateral rotation of the trunk over the femur on closed kinetic chain. it was concluded that external obturator muscle strain injuries can be mistaken for those related to the hip adductor muscles due to the reported pain area. clinical examination should be based on the athletes' report and functional tests, mainly for the hip rotator muscles. the mri scan was essential to the location, classification and evaluation of the injury size.
Altera??es hematológicas e sorológicas em eqüinos experimentalmente infectados com Babesia equi
Cunha, Cristina Wetzel da;Silva, Sergio Silva da;Osório, Bárbara Lícia;Dutra, Cristiana Lückemeyer;
Ciência Rural , 1998, DOI: 10.1590/S0103-84781998000200017
Abstract: in the present study the haematological and serological changes showed by horses experimentally infected with b. equi, during diffèrent infection stages and after chemotherapy parasite sterilization, are reported. ten horses clinically healthy and serologically negative for babesia spp were inoculated with b. equi and treated with babesicidal drugs during ascending parasitemia. after the treatment the horses were separated in two groups: carriers, which developed the chronic babesial infection, and sterilized horses, which the parasites were eliminated. during the whole experiment, hematocrit, parasitemia and antibody titers were monitored to characterize its dinamics in the acute and chronical babesial infection, as well as after elimination of parasites.
Ana Raquel Mano Meinerz,Marlete Brum Cleff,Patrícia da Silva Nascente,Luiza da Gama Osório
Ciência Animal Brasileira , 2010,
Abstract: The frequent occurrence of resistant isolated fungi againstantifungal drugs stimulated advances in the antifungigram techniques,which were standardized by CLSI. However, the methods have been inefficient and impractical to be executed in clinical laboratories. Within this context, commercial techniques have been developed, being ETEST one of them. ETEST has proved to be easier to execute when compared to the techniques approved by the CLSI. This study used the ETEST and the microdilution method, performed according to CLSI, for determining the in vitro susceptibility of isolates of Sporothrix schenckii against itraconazole. The CLSI uses RPMI 1640 medium and the reading of MIC after the period of incubation of 72h at 35oC. MIC was determined by the ETEST, being Sabouraud dextrose agar used as medium, and the reading performed after 72 hours of incubation at 35oC. The variance analysis, analyzed by T-paired test, did not demonstrate statistical differences among the CIM values obtained by the microdilution technique in broth (MIC among 0.219 and 0.875 μg/mL) and ETEST (MIC among 0.032 and 2.0 μg/mL). However, the correlation coefficient (R) was negative, probably because ofthe small number of samples. These results show the necessity offurther studies to assess the application of ETEST to evaluate thesusceptibility of S. schenckii against the itraconazol.
Ana Raquel Mano Meinerz,Patrícia da Silva Nascente,Luiz Filipe Damé Schuch,Renata Osório de Faria
Ciência Animal Brasileira , 2007,
Abstract: o felino doméstico com esporotricose representa um papel importante na transmiss o do S. schenckii a ou-tros animais e para o homem sendo crescente o aumento do número de casos zoonóticos da micose. o estudo des-creve três casos de esporotricose em gatos semidomicilia-dos, convivendo no mesmo bairro. os felinos apresenta-ram les es cutaneas ulceradas e crostosas, sendo realizada a confirma o do diagnóstico após a análise micológica com o isolamento do S. schenckii. o estudo ressalta a im-portancia dos felinos domésticos como propagadores da esporotricose para animais e para o homem. PALAVRAS-CHAVE: Esporotricose, zoonose, felino, Sporothrix schenckii. the domestic feline with sporotrichosis represents an important role in the transmission of the S. schenckii schenckii to other animal species and for the man being growing the increase of the number of zoonotic cases of the mycosis. the study reports three cases of feline sporo-trichosis in semi-domiciled cats, living together in the same neighborhood. the feline presented cutaneous ulcerated and crusted injuries being accomplished the confirmation of the diagnosis after the analysis micológica with the isolation of S. schenckii. the study emphasizes the importance of the domestic feline as diffuser of the sporotrichosis for other animals and for the man itself. KEY-WORDS: feline, sporotrichosis, zoonosis, Sporothrix schenckii.
Hepatotrophic factors reduce hepatic fibrosis in rats
Cogliati, Bruno;Pereira, Hélder de Moraes;Dagli, Maria Lúcia Zaidan;Parra, Osório Miguel;Silva, José Roberto Machado Cunha da;Hernandez-Blazquez, Francisco Javier;
Arquivos de Gastroenterologia , 2010, DOI: 10.1590/S0004-28032010000100014
Abstract: context: hepatic fibrosis occurs in response to several aggressive agents and is a predisposing factor in cirrhosis. hepatotrophic factors were shown to stimulate liver growth and to restore the histological architecture of the liver. they also cause an improvement in liver function and accelerate the reversion of fibrosis before it progresses to cirrhosis. objective: to test the effects of hepatic fibrosis solution composed by amino acids, vitamins, glucose, insulin, glucagon and triiodothyronine on hepatic fibrosis in rats. methods: fibrosis was induced in rats by gastric administration of dimethylnitrosamine (10 mg/kg) for 5 weeks. after liver biopsy, the rats received either hepatotrophic factors solution (40 mg/kg/day) or saline solution for 10 days by intraperitoneal injection. blood samples and liver fragments were collected for hepatic function analysis, standard histopathology evaluation, and morphometric collagen quantification. results: rats in the hepatotrophic factors group showed a decrease of the histopathological components of fibrosis and an increase of their hepatic mass (12.2%). there was no development of neoplasic lesions in both groups. compared with the saline group, the hepatotrophic factors group also had a decrease of blood levels of hepatic-lesion markers (ast, alt) and a decrease of collagen content in the portal spaces (31.6%) and perisinusoidal spaces (42.3%), as well as around the hepatic terminal vein (57.7%). thus, hepatotrophic factors administration in the portal blood promoted a regenerative hepatic response, with an overall reduction of the volumetric density of collagen, improved hepatic function, and a general improvement in the histopathological aspects of fibrosis. conclusion: taken together, these results suggest the potential therapeutic use of this hepatotrophic factors solution to treat chronic liver diseases.
Inimputabilidade: estudo dos internos do Instituto Psiquiátrico Forense Maurício Cardoso
Gauer, Gabriel Jose Chittó;Osório, Fernanda Correa;Cataldo Neto, Alfredo;Teixeira, Letícia;Caum, Mariane;Souza, Taís Amaral da Costa;Valle, Ver?nica;Cristófoli, Vivian;
Revista de Psiquiatria do Rio Grande do Sul , 2007, DOI: 10.1590/S0101-81082007000300008
Abstract: introduction: this study analyzed the profile of nonimputable inmates at instituto psiquiátrico forense maurício cardoso. methods: between april and august 2005, a total of 617 patients were selected for inclusion in the study. results: data analysis revealed that the inmates presented the following characteristics: mean age of 43.22 years, male (91.3%), defined profession (73.2%), completed elementary school (74.6%); without a partner (83.2%), crime against a person was the felony that generated nonimputability (62.1%), criminal records (58.3%), minimal sentence time was 1.76 years, serving it in criminal commitment (91.4%), mean time in prison of 9.33 years, under regime of progressive release (81.5%), previous hospitalization in other psychiatric institutions (55.2%), no previous probation measure (83.3%), diagnosis of schizophrenia and other psychotic disorders (61.4%). the following variables were statistically significant: among inmates with previous hospitalization, 61.9% had criminal history; and the higher the inmates' schooling level, the lower the level of criminal relapse. results showed a predominance of inmates with criminal records in patients with disorders related to substances (56.5%) and personality disorder (59.7%). it was also verified that 60.8% of those who committed crimes against a person were alcohol addicts, establishing a relation between violent criminality and disorders caused by substance misuse. conclusions: based on the analysis of the medical records of 617 inmates at instituto psiquiátrico forense maurício cardoso, this study aimed at presenting a map of individuals considered nonimputable in rio grande do sul.
Molecular and serological detection of Leishmania spp. in captive wild animals from Ilha Solteira, SP, Brazil
Jusi, Márcia Mariza Gomes;Starke-Buzetti, Wilma Aparecida;Oliveira, Trícia Maria Ferreira de Sousa;Tenório, Michely da Silva;Sousa, Lúcio de Oliveira de;Machado, Rosangela Zacarias;
Revista Brasileira de Parasitologia Veterinária , 2011, DOI: 10.1590/S1984-29612011000300008
Abstract: leishmaniasis is a zoonotic disease that affects 12 million people worldwide. several mammalian species can serve as a reservoir for this disease. dogs are the main reservoir for visceral leishmaniasis in urban areas, which has become a serious public health concern in brazil. the aim of this study was to evaluate the presence of leishmania spp. in captive wild animals from ilha solteira, s?o paulo, brazil. blood and various tissues samples were collected from animals of five different species: speothos venaticus, chrysocyon brachyurus, cerdocyon thous, pseudalopex vetulus, and procyon cancrivorus. antibodies against leishmania spp. were detected in three wild canids by indirect fluorescent antibody test (ifat) and enzyme-linked immunosorbent assay (elisa). pcr analyses of blood and bone marrow from all animals were negative, but leishmania dna was found in the tissues and skin of seropositive animals. positive pcr samples were also positive for leishmania donovani complex. analysis of sequenced pcr products showed similarities with different regions of leishmania (leishmania) infantum and leishmania (leishmania) chagasi kinetoplastids. measures to control visceral leishmaniasis in wild animals kept in brazilian zoos should be established, as no disease control programs are currently available.
CLSI broth microdilution method for testing susceptibility of Malasseziapachydermatis to thiabendazole
Nascente, Patrícia da Silva;Meinerz, Ana Raquel Mano;Faria, Renata Osório de;Schuch, Luiz Filipe Damé;Meireles,rio Carlos Araújo;Mello, Jo?o Roberto Braga de;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000200002
Abstract: thiabendazole, classified as antiparasitic and also used as an antifungal drug, can be found as otological solution indicated for treatment of parasitic and fungal external otitis in small animals. malassezia pachydermatis is a yeast recognized as a normal inhabitant on the skin and mucous membranes of dogs and cats. however, it is considered an opportunistic agent that causes external otitis and dermatitis in these animals. the aim of this study was to evaluate the in vitro effect of thiabendazole against 51 isolates of m. pachydermatis using the clsi broth microdilution method that has been adapted for this yeast species (nccls, 2002). based on this test, the minimum inhibitory concentrations (mic) of thiabendazol was calculated. subsequently, the susceptibility of each isolate against this antifungal was determined. it was observed that the mic of thiabendazole against m. pachydermatis ranged from 0.03 to > 4 μg/ml. a total of 13.7% of the isolates were found to be resistant, 47.1% were intermediate and 39.2% were sensitive to the drug. the rate of resistance of the yeasts against thiabendazole was similar to the results previously obtained with other antifungals, while the adapted broth microdilution technique used in this study proved to be efficient.
As raízes crist s da autonomia
Azambuja Letícia Erig Osório de
Cadernos de Saúde Pública , 2006,
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