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Search Results: 1 - 10 of 897 matches for " Tomohiro Yokozeki "
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Identification of Damage Parameters for Intralaminar Damage Modeling in Laminated Composites Considering Transverse Stress Effects  [PDF]
Rehs T. Gerrit, Shun Kokubo, Tomohiro Yokozeki
Open Journal of Composite Materials (OJCM) , 2017, DOI: 10.4236/ojcm.2017.74012
Abstract: The aim of this study is to develop an appropriate modeling methodology for the simulation of intralaminar damage in laminated composites under complex loadings. The intralaminar damages are modeled by stiffness reduction controlled by thermodynamic forces as defined in continuum damage mechanics model proposed by Ladevèze. The original method neglected transverse stress in elementary plies during the tensile tests of [45/?45]mS laminates, resulting in variations of the identified damage parameters of Ladevèze model. This study compared the identified damage parameters considering transverse stress effects with those based on the original method. The effect of transverse stress in the identification process on the damage modeling is discussed, and it is found that one of damage coupling parameters and the damage master curves significantly depend on consideration of transverse stress effects. Finally, it is demonstrated that experimental stiffness degradation is well simulated by the prediction using the identified parameters considering transverse stress effects.
Investigation of the Flexural Properties and Failure Behavior of Unidirectional CF/Nylon 6 and CF/Epoxy Composites  [PDF]
Yan Ma, Masahito Ueda, Tomohiro Yokozeki, Toshi Sugahara, Yuqiu Yang, Hiroyuki Hamada
Open Journal of Composite Materials (OJCM) , 2017, DOI: 10.4236/ojcm.2017.74016
Abstract: In this work, flexural properties and failure behavior of unidirectional (UD) carbon fiber reinforced polyamide 6 (CF/Nylon 6) and epoxy resin (CF/ Epoxy) laminates were investigated through three-point bending test. The mechanical properties and failure behavior of 0 and 90 degree CF/Nylon 6 and CF/Epoxy laminates were discussed based on the fiber volume fraction, fiber distribution, void content, interfacial properties, transversal tensile strength and fracture toughness. The effects of fiber volume fraction, fiber distribution, void content and their hybrid effect on the flexural properties were investigated. Step-by-step observation and scanning electron microscope observation of laminates after flexural tests were employed to analyze the fracture process.
Changes in Glutamine Synthetase Activity and Gene Expression During Storage of Moso Bamboo Shoot
Toshiyuki Matsui,Pankaj Kumar Bhowmik,Kyosuke Yokozeki
Asian Journal of Plant Sciences , 2004,
Abstract: Previously isolated cDNA clones encoding glutamine synthetase (GS, EC 6.3.1.2) genes from asparagus and broccoli were observed that the level of transcripts decreased in both plant species during storage. In order to identify factors regulating the postharvest deterioration of another important Asian vegetable bamboo (Phyllostachys edulis) shoot, the present study was conducted to isolate glutamine synthetase (pBA-GS) cDNA clone from a cDNA library prepared from harvested bamboo shoot using reverse transcriptase-polymerase chain reaction. The partial cDNA clone encodes an mRNA of 832 bp and the derived amino acid sequence is highly homologous to GS from rice, barely and maize. Northern blot analysis showed decrease level of pBA-GS mRNA until 9 d at 20 C, which coincided well with enzyme activity and ammonia accumulation. Results demonstrated that GS activity decrease during the postharvest storage of bamboo shoot which is a highly perishable vegetable like asparagus spears and broccoli head and is likely to be a critical feature of their postharvest physiology.
Glutamine Synthetase in Harvested Broccoli Head: Changes in Activity andGene Expression During Storage
Toshiyukii Matsui,Pankaj Kumar Bhowmik,Kyousuke Yokozeki
Asian Journal of Plant Sciences , 2004,
Abstract: Broccoli (Brassica oleracea L. cv. Hartland) cDNA clone coding the enzyme glutamine synthetase (GS; EC 6.3.1.2.) was isolated from a cDNA library prepared from broccoli head using reverse transcription-polymerase chain reaction (RT-PCR). The partial cDNA clone encodes an mRNA of 695 bp and the derived amino acid sequence is highly homologus to GS from rice cytosol, maize and European grape. GS has a central role in plant nitrogen metabolism and a key enzyme in the assimilation of ammonia. Broccoli head quality deteriorates rapidly after harvest and is associated with an increase in the ammonia content of the floret portions. In order to define the factors contributing to postharvest deterioration of broccoli head, the changes in GS activity and gene expression in the floret and branchlet portions of the broccoli were examined during storage. Northern blot analysis showed that the level of transcript of GS fluctuated in the floret and branchlet portions during storage. Expression of GS gene in the floret portion was observed more clearly but it was weak in the branchlet portion that might be due to the lower activity of GS in branchlet portions.
A cDNA Sequence Encoding Actin Gene in Moso Bamboo Shoot and its Phylogenetic Analysis
Toshiyuki Matsui,Pankaj Kumar Bhowmik,Kyosuke Yokozeki
Asian Journal of Plant Sciences , 2004,
Abstract: Actin is one of the most ubiquitous and conserved eukaryotic protein. All eukaryotic cells, from yeast to plants to animals, have an internal framework called the cytoskeleton. The plant actin cytoskeleton is central to many different sub-cellular processes and actin genes have been the focus of numerous scientific studies because of their involvement in basic cellular processes. In order to explain the structure and evolution of monocot actin gene family we have cloned and sequenced a 515 bp cDNA sequence from moso bamboo (Phyllostachys edulis) shoot. Phylogenetic analysis of the bamboo actin along with asparagus actin and those previously published in the database, indicates that monocots actin genes are highly conserved and actin multigene family underwent many gene duplication events early in the evolution of angiosperms.
Phenylalanine Ammonia-Lyase in Moso Bamboo Shoot: Molecular Cloning and Gene Expression During Storage
Toshiyuki Matsui,Pankaj Kumar Bhowmik,Kyosuke Yokozeki
Asian Journal of Plant Sciences , 2004,
Abstract: In plants, phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) catalyzes the conversion of L-phenylalanine to trans cinnamic acid in the first step of phenylpropanoid pathway. We previously isolated cDNA clones encoding PAL from asparagus spears and observed that the level of transcripts correlated well with increase enzyme activity and lignification. In order to identify factors regulating the postharvest deterioration of another important Asian vegetable bamboo (Phyllostachys edulis) shoot, we have now isolated PAL (pBA-PAL), cDNA clone from a cDNA library prepared from harvested bamboo shoot using reverse transcriptase-polymerase chain reaction. The partial cDNA clone encodes an mRNA of 527 bp and the derived amino acid sequence is highly homologous to PAL from rice, barely, maize and asparagus. Northern blot analysis showed increasing trend in the level of pBA-PAL mRNA reaching a peak after 3 d of storage at 20 C, which coincided well with enzyme activity. Our results demonstrate that during the postharvest storage of bamboo shoot PAL activity is regulated by increase level of pBA-PAL mRNA suggesting that the increase might be a response to the wounding associated with harvest.
A Wound-induced ACC Synthase Gene of Moso Bamboo Shoot
Toshiyuki Matsui,Kyosuke Yokozeki,Hiroyuki Inoue
Asian Journal of Plant Sciences , 2003,
Abstract: The present study was carried out to clone and sequence 1-aminocyclopropane-1-carboxylate synthase (ACC synthase, pBA-ACS) from a sliced moso bamboo shoot using reverse transcription and polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends-PCR (RACE-PCR). The cDNA clone of pBA-ACS was 1793 bp in length and contained a 5`-untranslated region of 126 bp, an open reading frame of 1446 bp encoding 482 amino acids and 3`-untranslated region of 221bp containing stop codone. The pBA-ACS was highly homologous to ACC synthase genes from rice, followed by apple and arabidopsis. In northern analysis, expression of pBA-ACS mRNA was enhanced in wounding tissue until 24 h and coincided with the peak of ACS synthase activity but disagreed with ethylene production. The ACC synthase activity suggested that the increase might be the response to the wounding associated with harvest.
3-D microstructure measurement method by using SEM
Arai Y.,Kanameishi S.,Yokozeki S.
EPJ Web of Conferences , 2010, DOI: 10.1051/epjconf/20100612001
Abstract: The three-dimensional measurement method by SEM has already been proposed by using the principle of shadow moiré. In this method, there are some troubles in order to perform a high-resolution analysis. A new method based on the principle of projection moiré by SEM is discussed to solve the troubles. In this paper, some shadows of the original grid on the surface of the object by back scattering electron beam are analyzed by using the spatial fringe analysis method and the new 3-D measurement based on a geometric analysis. The validity of the proposed method is confirmed by the experimental results. It is confirmed that the proposed method has a lateral high-resolution power.
Combined effects of knee extension strength, visual acuity, and knee-joint pain on older women’s gait  [PDF]
Tomohiro Demura, Shinichi Demura
Health (Health) , 2013, DOI: 10.4236/health.2013.53060
Abstract:

The purpose of this study was to examine the combined effects of knee extension strength (KES), visual acuity (VA), and knee joint pain (KP) on gait in 212 older women. Including, walking speed, cadence, were selected as gait parameters. Knee extension strength was measured by isometric knee extension strength, while knee joint pain and decreased visual acuity were evaluated by subjective judgment. The combine effect of KP and KES factors was examined. Stance time was significantly longer in persons with both-KP than in persons with no KP. In addition, people with superior KES had significantly greater values in walking speed, cadence, and step length, and lower values in stance time and walking angle than those with inferior KES. Furthermore, double support time showed that persons with both-KP have significantly greater values than persons with no or one-KP in the inferior KES group. Also, persons with the inferior KES had significantly greater values in persons with both-KP. The combine effect of KES and VA factors was examined. There are significant differences between the superior and the inferior KES groups. In conclusion, the elderly with both the factors of decreasing KES and both-KP, as compared to the elderly with just one of those factors, have markedly different gait properties.

Numerical Simulation of Liquid Crystal Flow Induced by Annihilation of a Pair of Defects  [PDF]
Shigeomi Chono, Tomohiro Tsuji
Open Journal of Fluid Dynamics (OJFD) , 2018, DOI: 10.4236/ojfd.2018.84022
Abstract: We investigate the flow induced by annihilation of a pair of defects in liquid crystals using the Doi theory with the Marrucci-Greco potential, in which the orientation state is described with the orientational distribution function. We have numerically studied both the transient behaviors of two defects with different structures and their velocity field, and estimated the magnitude of the induced velocity. A defect with positive strength moves faster than one with negative strength. The long-range order of the molecular orientation field has a large effect on the annihilation time, and the annihilation time is reduced by increasing the long-range order. We find that flows are induced during the annihilation of a pair of defects and that several vortices are generated in the vicinity of the defects. The maximum velocity is predicted to develop spatially between the two defects just after their annihilation in time. In our simulation, the maximum induced velocity reaches an order of 10 μm/s. The induced velocity increases with increasing long range-order and nematic potential strength.
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