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Search Results: 1 - 10 of 60179 matches for " Teh-Yang Hwa "
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Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses
Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou, Ya-Wen Tsai, Te-Chin Chu, Chun-Chieh A Shih, Wen-Hsiung Li, Ming-Che Shih
Biotechnology for Biofuels , 2011, DOI: 10.1186/1754-6834-4-24
Abstract: We have developed an efficient platform that uses a combination of transcriptomic and proteomic approaches to N. patriciarum to accelerate gene identification, enzyme classification and application in rice straw degradation. By conducting complementary studies of transcriptome (Roche 454 GS and Illumina GA IIx) and secretome (ESI-Trap LC-MS/MS), we identified 219 putative GH contigs and classified them into 25 GH families. The secretome analysis identified four major enzymes involved in rice straw degradation: β-glucosidase, endo-1,4-β-xylanase, xylanase B and Cel48A exoglucanase. From the sequences of assembled contigs, we cloned 19 putative cellulase genes, including the GH1, GH3, GH5, GH6, GH9, GH18, GH43 and GH48 gene families, which were highly expressed in N. patriciarum cultures grown on different feedstocks.These GH genes were expressed in Pichia pastoris and/or Saccharomyces cerevisiae for functional characterization. At least five novel cellulases displayed cellulytic activity for glucose production. One β-glucosidase (W5-16143) and one exocellulase (W5-CAT26) showed strong activities and could potentially be developed into commercial enzymes.Cellulosic ethanol produced by microbial fermentation from feedstocks has been proposed to replace fossil fuels in transportation. A key step in cellulosic ethanol production is to break down cellulose into glucose and hemicellulose into xylose, which can subsequently be converted into ethanol by fermentative microbes. Therefore, finding efficient cellulases is important to bioethanol production, as well as for hydrolyzing feedstocks into sugars in general. Neocallimastix species is one of the major anaerobic fungi in the rumen of water buffalo capable of efficiently digesting cellulosic biomass [1-4]. Such anaerobic fungi are potential sources for highly active cellulolytic enzymes that are useful for cellulose hydrolysis [5-7]. Plant cell wall degrading enzymes from rumen fungi such as Neocallimastix patriciarum may
Zfp322a Regulates Mouse ES Cell Pluripotency and Enhances Reprogramming Efficiency
Hui Ma,Hui Min Ng,Xiuwen Teh,Hu Li,Yun Hwa Lee,Yew Mei Chong,Yuin Han Loh,James J. Collins,Bo Feng,Henry Yang ,Qiang Wu
PLOS Genetics , 2014, DOI: doi/10.1371/journal.pgen.1004038
Abstract: Embryonic stem (ES) cells derived from the inner cell mass (ICM) of blastocysts are characterised by their ability to self-renew and their potential to differentiate into many different cell types. Recent studies have shown that zinc finger proteins are crucial for maintaining pluripotent ES cells. Mouse zinc finger protein 322a (Zfp322a) is expressed in the ICM of early mouse embryos. However, little is known regarding the role of Zfp322a in the pluripotency maintenance of mouse ES cells. Here, we report that Zfp322a is required for mES cell identity since depletion of Zfp322a directs mES cells towards differentiation. Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays revealed that Zfp322a binds to Pou5f1 and Nanog promoters and regulates their transcription. These data along with the results obtained from our ChIP-seq experiment showed that Zfp322a is an essential component of mES cell transcription regulatory network. Targets which are directly regulated by Zfp322a were identified by correlating the gene expression profile of Zfp322a RNAi-treated mES cells with the ChIP-seq results. These experiments revealed that Zfp322a inhibits mES cell differentiation by suppressing MAPK pathway. Additionally, Zfp322a is found to be a novel reprogramming factor that can replace Sox2 in the classical Yamanaka's factors (OSKM). It can be even used in combination with Yamanaka's factors and that addition leads to a higher reprogramming efficiency and to acceleration of the onset of the reprogramming process. Together, our results demonstrate that Zfp322a is a novel essential component of the transcription factor network which maintains the identity of mouse ES cells.
Induction of protective immunity in swine by recombinant bamboo mosaic virus expressing foot-and-mouth disease virus epitopes
Chung-Da Yang, Jia-Teh Liao, Chen-Yen Lai, Ming-Hwa Jong, Chi-Ming Liang, Yeou-Liang Lin, Na-Sheng Lin, Yau-Heiu Hsu, Shu-Mei Liang
BMC Biotechnology , 2007, DOI: 10.1186/1472-6750-7-62
Abstract: We engineered the BaMV as a vaccine vector expressing the antigenic epitope(s) of the capsid protein VP1 of foot-and-mouth disease virus (FMDV). The recombinant BaMV plasmid (pBVP1) was constructed by replacing DNA encoding the 35 N-terminal amino acid residues of the BaMV coat protein with that encoding 37 amino acid residues (T128-N164) of FMDV VP1.The pBVP1 was able to infect host plants and to generate a chimeric virion BVP1 expressing VP1 epitopes in its coat protein. Inoculation of swine with BVP1 virions resulted in the production of anti-FMDV neutralizing antibodies. Real-time PCR analysis of peripheral blood mononuclear cells from the BVP1-immunized swine revealed that they produced VP1-specific IFN-γ. Furthermore, all BVP1-immunized swine were protected against FMDV challenge.Chimeric BaMV virions that express partial sequence of FMDV VP1 can effectively induce not only humoral and cell-mediated immune responses but also full protection against FMDV in target animals. This BaMV-based vector technology may be applied to other vaccines that require correct expression of antigens on chimeric viral particles.Foot-and-mouth disease virus (FMDV) is the etiological agent of foot-and-mouth disease (FMD) that infects cloven-hoofed animals such as pigs, sheep and cattle and causes serious damage in the livestock industry [1]. Although conventional vaccines based on the chemically inactivated virus are effective against FMDV [2], outbreaks of FMD sometimes result from virus escaping from vaccine production units or from the use of improperly inactivated virus [2-4]. Alternative approaches to produce an effective and safe FMD vaccine are needed to replace inactivated virus-based vaccines.FMDV particles are composed of 60 copies of each of four capsid proteins termed VP1, VP2, VP3 and VP4, which are cleavage products of the capsid precursor polypeptide P1[5,6]. VP1, VP2 and VP3 form the outer capsid shell, whereas VP4 lines the interior surface [7]. Among these capsid
核心期刊館藏之建立 Core Collections of Journals
Mei-hwa Yang
Journal of Educational Media & Library Sciences , 1990,
Abstract: 無 Selectivity in the acquisition of primary journals is becoming increasingly important in view of the proliferation of journals and the pressure on acquisition budgets in Iibraries. Earlier studies have shown that in a given scientific subject, a relatively few primary journals contain a substantial proportion of journal literature, and the rest of the literature is scattered throughout a large number of journals only peripherally related to the subject. This project attempted to identify the core journals in Computer Science. Problems were discussed and characteristics of core journal collection were analyzed. The factors to be considered in any level of journal collection analysis include relation to curriculum and research; relation to the total collection; reputation of publishers and contributors; breadth and quantity of demand; cost; indexing in standard sources; accessibility from other sources. These and other bibliometric measures of journal significance such as impact factor are described and their relative advantages and weaknesses discussed.
大學圖書館之館藏管理 Collection Management in Academic Libraries
Mei-hwa Yang
Journal of Educational Media & Library Sciences , 1987,
Abstract: 無 Since the concept of Collection Planning arises, numerous articles dedicated to this subject can be seen in foreign literature. However, it was seldom discussed in this country. Therefore, this article tries to provide some thoughts in regard to Collection Management, which include collection development, collection control and conservation, and collection evaluation. In addition, it also introduces three models for collection management: i.e., the Concept Model for Collection Analysis and Measurement, the Structural/ functional System Model, and the North American Collection Inventory Project, which, the author believes, can be profitably adopted by libraries here in Taiwan.
Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response
Pei-Ming Chu, Shih-Hwa Chiou, Tsann-Long Su, Yi-Jang Lee, Li-Hsin Chen, Yi-Wei Chen, Sang-Hue Yen, Ming-Teh Chen, Ming-Hsiung Chen, Yang-Hsin Shih, Pang-Hsien Tu, Hsin-I Ma
Radiation Oncology , 2011, DOI: 10.1186/1748-717x-6-7
Abstract: The clonogenic assay was used to determine the IC50 and radiosensitivity of human glioma cell lines (U87MG, U251MG and GBM-3) following BO-1051. DNA histogram and propidium iodide-Annexin V staining were used to determine the cell cycle distribution and the apoptosis, respectively. DNA damage and repair state were determined by γ-H2AX foci, and mitotic catastrophe was measure using nuclear fragmentation. Xenograft tumors were measured with a caliper, and the survival rate was determined using Kaplan-Meier method.BO-1051 inhibited growth of human gliomas in a dose- and time-dependent manner. Using the dosage at IC50, BO-1051 significantly enhanced radiosensitivity to different extents [The sensitizer enhancement ratio was between 1.24 and 1.50 at 10% of survival fraction]. The radiosensitive G2/M population was raised by BO-1051, whereas apoptosis and mitotic catastrophe were not affected. γ-H2AX foci was greatly increased and sustained by combined BO-1051 and γ-rays, suggested that DNA damage or repair capacity was impaired during treatment. In vivo studies further demonstrated that BO-1051 enhanced the radiotherapeutic effects on GBM-3-beared xenograft tumors, by which the sensitizer enhancement ratio was 1.97. The survival rate of treated mice was also increased accordingly.These results indicate that BO-1051 can effectively enhance glioma cell radiosensitivity in vitro and in vivo. It suggests that BO-1051 is a potent radiosensitizer for treating human glioma cells.Malignant gliomas account for approximately 30% of all intracranial tumors, and of them, glioblastoma multiforme (GBM) is considered as the most frequent and aggressive type. Removal of GBM by surgical resection is usually not feasible due to the highly diffuse infiltrative growth and recurrence rate [1]. A multicenter study has shown that addition of concurrent temozolomide (TMZ) to radical radiation therapy improves the survival in patients who suffered from GBM [2,3]. These studies have demonstrated
Measures of Association for Identifying MicroRNA-mRNA Pairs of Biological Interest
Vivek Jayaswal, Mark Lutherborrow, Yee Hwa Yang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0029612
Abstract: MicroRNAs are a class of small non-protein coding RNAs that play an important role in the regulation of gene expression. Most studies on the identification of microRNA-mRNA pairs utilize the correlation coefficient as a measure of association. The use of correlation coefficient is appropriate if the expression data are available for several conditions and, for a given condition, both microRNA and mRNA expression profiles are obtained from the same set of individuals. However, there are many instances where one of the requirements is not satisfied. Therefore, there is a need for new measures of association to identify the microRNA-mRNA pairs of interest and we present two such measures. The first measure requires expression data for multiple conditions but, for a given condition, the microRNA and mRNA expression may be obtained from different individuals. The new measure, unlike the correlation coefficient, is suitable for analyzing large data sets which are obtained by combining several independent studies on microRNAs and mRNAs. Our second measure is able to handle expression data that correspond to just two conditions but, for a given condition, the microRNA and mRNA expression must be obtained from the same set of individuals. This measure, unlike the correlation coefficient, is appropriate for analyzing data sets with a small number of conditions. We apply our new measures of association to multiple myeloma data sets, which cannot be analyzed using the correlation coefficient, and identify several microRNA-mRNA pairs involved in apoptosis and cell proliferation.
大學圖書館西文期刊之評量 | The Evaluation of Western Periodical Collection in University Libraries (Text in Chinese)
楊美華 Mei-Hwa Yang
Journal of Library and Information Science , 1988,
Abstract: 頁次:208-231
我國圖書資訊學教育的省思 | Reflections on Library and Information Science Education in Taiwan
楊美華 Mei-Hwa Yang
Journal of Library and Information Science , 2004,
Abstract: 頁次:46-58
網路時代公共圖書館的新思維:尋找知識服務的切入點
楊美華 Mei-Hwa Yang
Journal of Library and Information Science , 2001,
Abstract: 頁次:89-90
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