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Search Results: 1 - 10 of 1598 matches for " Takahiro Funatsu "
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Analysis of the Factors for Displacement of Earth Retaining Wall with Twin-Stepped Construction  [PDF]
Seiya Sakuma, Kazuki Maehara, Takahiro Funatsu, Akihiro Hamanaka, Takashi Sasaoka, Hideki Shimada
World Journal of Engineering and Technology (WJET) , 2019, DOI: 10.4236/wjet.2019.71007
Abstract: The demand for specific earth retaining wall method is increasing, along with the advancement and overcrowding of underground space use, such as the?presence of close structures, in an urban area. The method is named stepped-twin?retaining wall. The feature of this method is to have inner and outer retaining walls and excavate the ground by two-step in order to minimize the effect of the excavation on neighboring existing structures. However, the design of the earth retaining wall is currently carried out by individual engineers based on their own experience. Therefore, it is crucial that the standard method of retaining wall using a two-step construction is established. As the first step toward the standardization, evaluation of factors affecting the ground behavior of the earth retaining wall was carried out. In particular, we picked up four major considerable factors, which are horizontal distance between the outer and inner walls, depth of outer wall embedment, mechanical properties of soil. The evaluation was done by using two dimensional FEM analysis and the results were summarized to make clear the effect of each factor.
Development of Surgical Treatment for Vitreous Disease  [PDF]
Tatsuya Mimura, Hidetaka Noma, Hideharu Funatsu
Surgical Science (SS) , 2012, DOI: 10.4236/ss.2012.311101
Abstract: Vitreous surgery has improved remarkably with various advances in surgical instruments and techniques. The two most important breakthrough in recent years have been the introduction of small-gauge pars plana vitrectomy (PPV) systems and new illumination devices. Compared with traditional 20-gauge PPV, sutureless small-gauge PPV appears to be safer, with a shorter operating time, less postoperative inflammation, less patient discomfort, and more rapid recovery of visual acuity. This article reviews recent progress in the surgical management of vitreous disease with a historical perspective.
Label-Free Quantification of MicroRNAs Using Ligase-Assisted Sandwich Hybridization on a DNA Microarray
Taro Ueno, Takashi Funatsu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0090920
Abstract: MicroRNAs (miRNAs) can be used as biomarkers for cancer and other human diseases; therefore, high-throughput and reliable miRNA-quantification methods are required to exploit these markers for diagnostic testing. In this report, we describe the construction of a platform for miRNA-quantification using ligase-assisted sandwich hybridization (LASH) without miRNA-labeling. T4 DNA ligase was used to compensate for the low affinity between miRNAs and two short complementary DNA probes, and it improved the hybridization yield ~50,000 times. The LASH assay enabled synthesized miR-143 to be quantified at concentrations ranging from 30 fM to 30 pM. The LASH assay could also quantify endogenous miR-143 released from cultured cells as well as some miRNAs in total RNAs derived from blood. Furthermore, multi-color detection enabled us to distinguish between the highly homologous miR-141 and miR-200a. This simple label-free quantification technique is an easy-to-use approach that can be applied to disease diagnosis.
The genetic architecture of insecticide resistance within a natural population of Drosophila melanogaster  [PDF]
Takahiro Miyo
Open Journal of Genetics (OJGen) , 2012, DOI: 10.4236/ojgen.2012.22013
Abstract: The dynamics of genetic variation in susceptibility to insecticides within a natural population of Drosophila melanogaster (Meigen) at Katsunuma (Yamanashi Prefecture, Japan) was examined. Two resistance factors for three organophosphate insecticides (OPs), a resistant-type acetylcholinesterase (AChE) and a cytochrome P450 monooxygenase (cytochrome P450), have already been suggested to be involved within the Katsunuma population. In this study, genetic variances were estimated for susceptibility to other classes of chemicals than OPs, permethrin (a pyrethroid) and dichloro-diphenyl-trichloroethane (DDT; an organo-chlorine), which existed simultaneously with genetic variances for susceptibility to OPs. Analyses of variance for susceptibility to permethrin and DDT showed highly significant variation among isofemale lines from the Katsunuma population, and the genetic variances for susceptibility to each insecticide fluctuated differently during this period. The impacts of fluctuations of genetic variation in susceptibility to one class of insecticides on genetic variation in susceptibility to other classes of insecticides existing simultaneously within the natural population were discussed.
Is It Wrong to Apply the Intrinsic Rate of Natural Increase to Individuals and Compare It among Genotypes?  [PDF]
Takahiro Miyo
Open Journal of Genetics (OJGen) , 2018, DOI: 10.4236/ojgen.2018.81001
Abstract: In order to gain insights into the seasonal dynamics of genetic variation in insecticide resistance within a natural population of Drosophila melanogaster during population growth, which we considered the most important ecological factor there, we conducted a series of genetic analyses of resistance factors involved in that population and compared individual-based intrinsic rates of natural increase among resistance genotypes. However, some researchers have argued that it is a misconception to apply the intrinsic rate of natural increase to individuals, because it is a population parameter. We consider that their criticisms were incorrect. In this article, I described our research briefly and set forth the reasons why we conducted these studies.
Elevated Root-Zone Temperature Modulates Growth and Quality of Hydroponically Grown Carrots  [PDF]
Masaru Sakamoto, Takahiro Suzuki
Agricultural Sciences (AS) , 2015, DOI: 10.4236/as.2015.68072
Abstract: Air and soil temperatures strongly influence the growth and quality of crops. However, in root vegetables, such as carrot, few experiments aimed at regulating growth and quality by manipulating root-zone temperature have been reported. We investigated the effect of root-zone temperatures (20°C, 25°C, 29°C, and 33°C) on carrot growth and components using a hydroponic system. High root-zone temperatures for 14 days reduced shoot and rootgrowth and water content. In contrast, total phenolic compounds and soluble-solid content increased in tap roots under high-temperature treatment. Root oxygen consumption was upregulated after 7 days under high-temperature treatment. These results suggest that high root-zone temperatures induce drought-like stress responses that modulate carrot biomass and components. High root-zone temperature treatments administered to hydroponically grown crops may be a valuable tool for improving and increasing the quality and value of crops.
Effect of Root-Zone Temperature on Growth and Quality of Hydroponically Grown Red Leaf Lettuce (Lactuca sativa L. cv. Red Wave)  [PDF]
Masaru Sakamoto, Takahiro Suzuki
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.614238
Abstract: Soil temperature influences crop growth and quality under field and greenhouse conditions; however, precise investigation using controlled cultivation systems is largely lacking. We investigated effects of root-zone temperatures on growth and components of hydroponically grown red leaf lettuce (Lactuca sativa L. cv. Red Wave) under a controlled cultivation system at 20°C. Compared with ambient root-zone temperature exposure, a 7-day low temperature exposure reduced leaf area, stem size, fresh weight, and water content of lettuce. However, root-zone heating treatments produced no significant changes in growth parameters compared with ambient conditions. Leaves under low root-zone temperature contained higher anthocyanin, phenols, sugar, and nitrate concentrations than leaves under other temperatures. Root oxygen consumption declined with low temperature root exposure, but not with root heating. Leaves of plants under low rootzone temperature showed hydrogen peroxide production, accompanied by lipid peroxidation. Therefore, low temperature root treatment is suggested to induce oxidative stress responses in leaves, activating antioxidative secondary metabolic pathways.
Synergistic Effects of a Night Temperature Shift and Methyl Jasmonate on the Production of Anthocyanin in Red Leaf Lettuce  [PDF]
Masaru Sakamoto, Takahiro Suzuki
American Journal of Plant Sciences (AJPS) , 2017, DOI: 10.4236/ajps.2017.87106
Abstract: The production of a secondary metabolite such as anthocyanin is coordinately regulated by plant intrinsic factors and influenced by multiple environmental factors. In red leaf lettuce, the red pigment component anthocyanin is important for the commercial value of the crop, but its synchronous regulation by multiple factors is not well understood. Here, we examined the synergistic effects of a night temperature shift and methyl jasmonate (MJ) on the production of anthocyanin in red leaf lettuce. Low or high night temperature treatment for 3 days just before harvesting induced the production of anthocyanin without affecting plant biomass. Temperature-dependent activation of anthocyanin accumulation was accelerated by treating with MJ. Night temperature shifts and MJ triggered oxidative stresses in leaves, as indicated by hydrogen peroxide accumulation and lipid peroxidation. Interestingly, these oxidative stresses were more evident in leaves simultaneously treated with both a high night temperature and MJ. The activity of the superoxide dismutase (SOD) was increased alongside the elevation of oxidative stress. Taken together, these results indicate that the combined treatment of a night temperature shift with MJ may accelerate anthocyanin production by increasing the levels of oxidative stress to the leaves of red leaf lettuce.
Single-Molecule Fluorescence Polarization Study of Conformational Change in Archaeal Group II Chaperonin
Ryo Iizuka, Taro Ueno, Nobuhiro Morone, Takashi Funatsu
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0022253
Abstract: Group II chaperonins found in archaea and in eukaryotic cytosol mediate protein folding without a GroES-like cofactor. The function of the cofactor is substituted by the helical protrusion at the tip of the apical domain, which forms a built-in lid on the central cavity. Although many studies on the change in lid conformation coupled to the binding and hydrolysis of nucleotides have been conducted, the molecular mechanism of lid closure remains poorly understood. Here, we performed a single-molecule polarization modulation to probe the rotation of the helical protrusion of a chaperonin from a hyperthermophilic archaeum, Thermococcus sp. strain KS-1. We detected approximately 35° rotation of the helical protrusion immediately after photorelease of ATP. The result suggests that the conformational change from the open lid to the closed lid state is responsible for the approximately 35° rotation of the helical protrusion.
Visual acuity and foveal thickness after vitrectomy for macular edema associated with branch retinal vein occlusion: a case series
Hidetaka Noma, Hideharu Funatsu, Tatsuya Mimura, Shuichiro Eguchi, Katsunori Shimada
BMC Ophthalmology , 2010, DOI: 10.1186/1471-2415-10-11
Abstract: A series of 54 consecutive patients (54 eyes) with branch retinal vein occlusion was studied prospectively. All patients underwent pars plana vitrectomy for treatment of macular edema. Best corrected visual acuity and retinal thickness (examined by optical coherence tomography) were assessed before and after surgery. The level of vascular endothelial growth factor in vitreous fluid harvested at operation was determined. Patients were followed for at least 6 months postoperatively.Both the visual acuity and the retinal thickness showed significant improvement at 6 months postoperatively (P = 0.0002 and P < 0.0001, respectively). The vitreous level of vascular endothelial growth factor was significantly higher in patients who showed less improvement of visual acuity compared with those who had a better visual prognosis (p = 0.0135). In contrast, a high vitreous level of vascular endothelial growth factor was associated with greater improvement of macular edema (p = 0.0064).These results suggest that the vitreous level of vascular endothelial growth factor might influence the visual prognosis and the response of macular edema to vitrectomy in patients with branch retinal vein occlusion.Branch retinal vein occlusion (BRVO) is a common retinal vascular disease that often causes macular edema, which is the main reason for visual impairment in these patients[1,2]. We have been investigating various factors that might be involved in the pathogenesis of macular edema associated with BRVO. We recently reported that the vitreous level of vascular endothelial growth factor (VEGF) is elevated in BRVO patients with macular edema, and that vitreous VEGF levels are correlated with the non-perfused area of the retina and with the severity of macular edema[3,4]. VEGF directly increases vascular permeability[5], and VEGF expression by retinal glial cells is upregulated due to hypoxia[6]. Accordingly, VEGF may contribute to the occurrence of macular edema in patients with BRVO, and a d
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