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Search Results: 1 - 10 of 28292 matches for " Tae-Jin Youn "
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In-silico and In-vitro based studies of Streptomyces peucetius CYP107N3 for oleic acid epoxidation
Tae-Jin Oh
BMB Reports , 2012,
Abstract: Certain members of the cytochromes P450 superfamily metabolizepolyunsaturated long-chain fatty acids to several classesof oxygenated metabolites. An approach based on in silicoanalysis predicted that Streptomyces peucetius CYP107N3might be a fatty acid-metabolizing enzyme, showing highhomology with epoxidase enzymes. Homology modeling anddocking studies of CYP107N3 showed that oleic acid can fitdirectly into the active site pocket of the double bond of oleicacid within optimum distance of 4.6 from the Fe. In order toconfirm the epoxidation activity proposed by in silico analysis,a gene coding CYP107N3 was expressed in Escherichia coli.The purified CYP107N3 was shown to catalyze C9-C10epoxidation of oleic acid in vitro to 9,10-epoxy stearic acidconfirmed by ESI-MS, HPLC-MS and GC-MS spectral analysis.
The Occurrence of Warfarin-Related Nephropathy and Effects on Renal and Patient Outcomes in Korean Patients
Jung Nam An, Shin Young Ahn, Chang-Hwan Yoon, Tae-Jin Youn, Moon-Ku Han, Sejoong Kim, Ho Jun Chin, Ki Young Na, Dong-Wan Chae
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0057661
Abstract: Background Warfarin-related nephropathy (WRN) is a recently described disease entity, in which excessive warfarinization (international normalized ratio (INR) >3.0) causes acute kidney injury. Previous reports regarding WRN included few Asian patients who might have differed from the western WRN patients in terms of genetic and environmental factors. Methods During the period of March 2003 to December 2011, the data about a total of 1297 patients who had serum creatinine (sCr) level measured within 1 week after INR >3.0 and within 6 months before INR >3.0 was analyzed through the retrospective review of electronic medical records of a single tertiary hospital in Korea. Result WRN developed in 19.3% of patients having excessive warfarinization. The incidence was higher in the chronic kidney disease (CKD) group than the non-CKD group. The risk of WRN increased as the basal serum albumin level decreased and was strongly associated with highest quartile serum AST level at post INR elevation and the presence of congestive heart failure. But the presence of atrial fibrillation was protective against the development of WRN. Neither the presence of CKD nor basal estimated glomerular filtration rate (eGFR) was an independent risk factor for WRN. Despite no difference in the basal sCr level, the sCr level was higher in patients with WRN than those without WRN after follow-up. The mortality rates were also higher in patients with WRN. Conclusions WRN developed in 19.3% of patients having excessive warfarinization. A lower basal serum albumin, highest quartile serum AST level at post INR elevation, and congestive heart failure were associated with the occurrence of WRN. The development of WRN adversely affected renal and patient outcomes.
Performance Analysis of IEEE 802.11 DCF under Nonsaturation Condition
Yutae Lee,Min Young Chung,Tae-Jin Lee
Mathematical Problems in Engineering , 2008, DOI: 10.1155/2008/574197
Abstract: Carrier sense multiple access with collision avoidance (CSMA/CA) methods are considered to be attractive MAC protocols for wireless LANs. IEEE 802.11 distributed coordination function (DCF) is a random channel access scheme based on CSMA/CA method and the binary slotted exponential backoff procedure to reduce the packet collision. In this paper, we propose a new analytical model for a nonsaturated IEEE 802.11 DCF network and evaluate its performance. We verify our model using simulations and show that our results agree with the simulations.
Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants
Tae-Jin Kang, Moon-Sik Yang
BMC Biotechnology , 2004, DOI: 10.1186/1472-6750-4-20
Abstract: We developed new rapid and reliable genomic DNA extraction method. With our developed method, plant genomic DNA extraction could be performed within 30 min. The method was as follows. Plant tissue was homogenized with salt DNA extraction buffer using hand-operated homogenizer and extracted by phenol:chloroform:isoamyl alcohol (25:24:1). After centrifugation, the supernatant was directly used for DNA template for PCR, resulting in successful amplification for RAPD from various sources of plants and specific foreign genes from transgenic plants. After precipitating the supernatant, the DNA was completely digested by restriction enzymes.This DNA extraction procedure promises simplicity, speed, and efficiency, both in terms of time and the amount of plant sample required. In addition, this method does not require expensive facilities for plant genomic DNA extraction.Molecular biological studies of plants require high-quality DNA. Several DNA extraction procedures for isolating genomic DNA from various plant sources have been described, including the salt extraction method and the cetyltrimethyl ammonium bromide (CTAB) method [1] and its modifications [2,3]. The need for a rapid and simple procedure is urgent, especially when hundreds of samples need to be analyzed.Most methods require the use of liquid nitrogen [4] or freeze-drying (lyophilization) [5,6] of tissue for the initial grinding, and these processes are unavailable in many regions of the world. After grinding the tissues in various extraction buffers, DNA is extracted with phenol-chloroform, or the extract is dialyzed against EDTA and a buffered Tris-HCl solution [7]. After extraction, the aqueous phase is concentrated, either by ethanol or isopropanol precipitation [8,9], or with microconcentrators (e.g., the Wizard genomic DNA purification system; Promega, USA). However, these methods are not time efficient for consistently obtaining PCR-quality DNA from calluses and plants, since they require that the tissu
Multi-phase Design Optimization of a Long Range Aerial Lift Boom Structure
Henry Panganiban,In-Gyu Ahn,Tae-Jin Chung
Lecture Notes in Engineering and Computer Science , 2012,
Control of De-Lamination Phenomena in LTCC Zero-Shrinkage by Glass Infiltration Method
Dong-Hun Yeo,Tae-Jin Jo
Transactions on Electrical and Electronic Materials , 2012,
Abstract: A zero-shrinkage sintering process in which the shrinkage of x-y axis is controlled to be zero is in great demanddue to the trend of high integration in the ceramic modules. Among the zero-shrinkage sintering processes that areavailable, the proposed glass infiltration method where the viscous but fluidic glass infiltrates of the Al2O3 particles inthe structure of Al2O3/glass/Al2O3 during firing is one of the applicable methods. However, the above proposed glassinfiltration method has the problem of the warpage-like delamination. This occurred at the outermost surface of themultiple-bundle substrate. It is thought that the decomposed gas rapidly expands in low viscous glass to create vacantspace. To solve this problem, the vacant space was tamped with Al2O3 particles to lead to the actual improvement ofthe sintered properties. With 15 wt% of tamping Al2O3 particles in glass, most of the vacant space disappeared. Fullydensified zero-shrinkage substrate without delamination can be obtained.
Guest Editorial
Masato Oguchi,Hyung-Jin Choi,Tae-Jin Lee,Seong Keun Oh
Journal of Networks , 2011, DOI: 10.4304/jnw.6.3.341-342
Abstract: This special issue of the Journal of Networks consists of six selected papers from the 4th International Conference on Ubiquitous Information Management and Communication (ICUIMC 2010) held in January 14th - 15th. Among them, six qualified papers have been selected as the papers for the special issue of the Journal of Networks, which covers the recent trends in wireless communications and networking. This issue includes various research field in communications and networking technologies. As one of the recent hot topics in this field, "A Communication Protocol for Sensor Database Construction by Rounding Sink" by Tomoki Yoshihisa and Shojiro Nishio, presents a model and simulation-based analytical results in wireless sensor networks. The rounding sink approach has been presented and studied, in which rounding sinks repeatedly wander about in the area to collect data from each sensor. "An Efficient Mutual Authentication and Access Control Scheme for Wireless Sensor Networks in Healthcare" by Xuan Hung Le, Murad Khalid, Ravi Sankar, and Sungyoung Lee, also discusses an issue in wireless sensor networks. To achieve the protection of data confidentiality and patient privacy in healthcare applications using wireless sensors, a secure, lightweight public key-based security scheme is proposed and evaluated by simulation. "An Energy-efficient Multi-candidate Greedy Routing Scheme in Wireless Sensor Networks" by Dang Tu Nguyen, Wook Choi, Minh Thiep Ha, and Hyunseung Choo, introduces novel multi-candidate greedy routing scheme in wireless sensor networks. According to the simulation results, their method increases the routing performance significantly in terms of packet delivery latency and energy efficiency. A fundamental technology for wireless communications is included in this issue as well. "The Enhanced Decoding Method for QO-SFBC System in Frequency Selective Fading Channel Environment" by Young-il Min, Hwan-min Park, Jun-hee Jang, Keun-dea Kim, and Hyung-jin Choi, proposes a decoding method to improve the performance of quasi orthogonal-space frequency block coding in frequency selective fading channel environment. An analysis and evaluation results by simulation show that the method can provide better performance compared with the conventional methods. This issue also deals with the field of image processing, which can be applied in the various Internet environments. "Image Retrieval with Generative Model for Typicality" by Taro Tezuka and Akira Maeda, discusses the problem to find the most typical image that depicts the object specified by a query. Thei
Efficacies of the new Paclitaxel-eluting Coroflex Please? Stent in percutaneous coronary intervention; comparison of efficacy between Coroflex Please? and Taxus? (ECO-PLEASANT) trial: study rationale and design
Jae-Bin Seo, Hui-Kyung Jeon, Kyung-Woo Park, Jong-Seon Park, Jang-Ho Bae, Sang-Wook Kim, Keon-Woong Moon, Jae-Woong Choi, Sang-Gon Lee, Woo-Young Chung, Tae-Jin Youn, Soo-Joong Kim, Doo-Il Kim, Byung-Ok Kim, Min-Su Hyon, Keum-Soo Park, Tae-Joon Cha, Hweung-Kon Hwang, Seung-Ho Hur, Hyo-Soo Kim
Trials , 2009, DOI: 10.1186/1745-6215-10-98
Abstract: In the comparison of Efficacy between COroflex PLEASe? ANd Taxus? stent(ECO-PLEASANT) trial, approximately 900 patients are being prospectively and randomly assigned to the either type of Coroflex Please? stent and Taxus Liberte? stent via web-based randomization. The primary endpoint is clinically driven target vessel revascularization at 9 months. The secondary endpoints include major cardiac adverse events, target vessel failure, stent thrombosis and angiographic efficacy endpoints.The ECO-PLEASANT trial is the study not yet performed to directly compare the efficacy and safety of the Coroflex Please? versus Taxus Liberte? stent. On the basis of this trial, we will be able to find out whether the Coroflex Please? stent is non-inferior to Taxus Liberte? stent or not.ClinicalTrials.gov number, NCT00699543.Previous randomized trials have shown the efficacy of a slow-release polymeric sirolimus-eluting stent (Cypher?, Cordis, Warren, NJ, USA), paclitaxel-eluting stent (Taxus?, Boston Scientific, Natick, MA, USA), and zotarolimus-eluting stent (Endeavor?, Medtronic, Minneapolis, MN, USA) over bare metal stents in reducing neointimal hyperplasia, late luminal loss, and angiographic restenosis leading to decreased target lesion revascularization [1-11] The Paclitaxel-eluting Coroflex Please? stent is a newly developed drug eluting stent using the Coroflex? stent platform combined with the drug paclitaxel contained in a polymer coating[12]In the PECOPS I, which was one-arm observational study, the results of Coroflex Please? stent were within the range of other Paclitaxel-eluting coronary stents [12,13] Compared with binary restenosis rate of 7.9% in Taxus IV trial, Coroflex? Please stent showed 7.8% of restenosis rate[7] The 3.1% of 30 day MACE rate is within the range of other trials with stents eluting Paclitaxel or Sirolimus. The 6 month MACE rates in PECOPS I were 8.0%, which was similar to 7.8%, and 8.5% in Taxus II MR and SR, respectively[6] In Taxus IV, 9 month f
MCBT: Multi-Hop Cluster Based Stable Backbone Trees for Data Collection and Dissemination in WSNs
Inyoung Shin,Moonseong Kim,Matt W. Mutka,Hyunseung Choo,Tae-Jin Lee
Sensors , 2009, DOI: 10.3390/s90806028
Abstract: We propose a stable backbone tree construction algorithm using multi-hop clusters for wireless sensor networks (WSNs). The hierarchical cluster structure has advantages in data fusion and aggregation. Energy consumption can be decreased by managing nodes with cluster heads. Backbone nodes, which are responsible for performing and managing multi-hop communication, can reduce the communication overhead such as control traffic and minimize the number of active nodes. Previous backbone construction algorithms, such as Hierarchical Cluster-based Data Dissemination (HCDD) and Multicluster, Mobile, Multimedia radio network (MMM), consume energy quickly. They are designed without regard to appropriate factors such as residual energy and degree (the number of connections or edges to other nodes) of a node for WSNs. Thus, the network is quickly disconnected or has to reconstruct a backbone. We propose a distributed algorithm to create a stable backbone by selecting the nodes with higher energy or degree as the cluster heads. This increases the overall network lifetime. Moreover, the proposed method balances energy consumption by distributing the traffic load among nodes around the cluster head. In the simulation, the proposed scheme outperforms previous clustering schemes in terms of the average and the standard deviation of residual energy or degree of backbone nodes, the average residual energy of backbone nodes after disseminating the sensed data, and the network lifetime.
Saposin C promotes survival and prevents apoptosis via PI3K/Akt-dependent pathway in prostate cancer cells
Tae-Jin Lee, Oliver Sartor, Ronald B Luftig, Shahriar Koochekpour
Molecular Cancer , 2004, DOI: 10.1186/1476-4598-3-31
Abstract: We have recently reported that prosaposin is expressed at a higher level by androgen-independent (AI) prostate cancer cells as compared to androgen-sensitive prostate cancer cells or normal prostate epithelial and stromal cells. In addition, we have demonstrated that a synthetic peptide (prosaptide TX14A), derived from the trophic sequence of the saposin C domain of prosaposin, stimulated cell proliferation, migration and invasion and activated the MAPK signaling pathway in prostate cancer cells. The biological significances of saposin C and prosaposin in prostate cancer are not known.Here, we report that saposin C, in a cell type-specific and dose-dependent manner, acts as a survival factor, activates the Akt-signaling pathway, down-modulates caspase-3, -7, and -9 expression and/or activity, and decreases the cleaved nuclear substrate of caspase-3 in prostate cancer cells under serum-starvation stress. In addition, prosaptide TX14A, saposin C, or prosaposin decreased the growth-inhibitory effect, caspase-3/7 activity, and apoptotic cell death induced by etoposide. We also discovered that saposin C activates the p42/44 MAP kinase pathway in a pertussis toxin-sensitive and phosphatidylinositol 3-kinase (PI3K) /Akt-dependent manner in prostate cancer cells. Our data also show that the anti-apoptotic activity of saposin C is at least partially mediated via PI3K/Akt signaling pathway.We postulate that as a mitogenic, survival, and anti-apoptotic factor for prostate cancer cells, saposin C or prosaposin may contribute to prostate carcinogenesis at its early androgen-dependent or metastatic AI state.Androgens, growth factors, neuropeptides, and other trophic agents are involved in normal and neoplastic growth of the prostate. Prosaposin is the intracellular precursor of four lysosomal glycoproteins, saposins A-D, that are involved in lysosomal hydrolysis of sphingolipids. These saposins, through their interaction with glycosphingolipid hydrolases and their substrates, inc
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