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Search Results: 1 - 10 of 871 matches for " Suzete Cleusa;Sabino "
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Compara??o de três protocolos distintos para extra??o de RNA de amostras fixadas em formalina e emblocadas em parafina
Gouveia, Gisele Rodrigues;Ferreira, Suzete Cleusa;Sabino, Ester Cerdeira;Siqueira, Sheila Aparecida Coelho;Pereira, Juliana;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2011, DOI: 10.1590/S1676-24442011000600012
Abstract: introduction: formalin fixed paraffin embedded (ffpe) tissues are an important sample source for retrospective studies. despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests once it fragments and chemically modifies nucleic acids, particularly rna. objective: to compare the efficiency of three different rna extraction protocols for gene expression analysis of ffep tissues. material and methods: rna was extracted from ffpe samples of human lymph by means of ambion and arcturus bioscience kits and the classical trizol method. after extraction, rna was quantified and tested for amplification through real time polymerase chain reaction (rt-pcr) using glyceraldehydes-3 phosphate dehydrogenase (gapdh) endogenous gene primers. discussion/conclusion: all the protocols produced sufficient and adequate amounts of total rna. however, only protocols using arcturus and ambion kits generated suitable rna for pcr amplification.
Near full-length genome analysis of low prevalent human immunodeficiency virus type 1 subclade F1 in S?o Paulo, Brazil
Sabri Sanabani, évelyn Pastena, Walter Neto, Claudia C Barreto, Kelly T Ferrari, Erika MN Kalmar, Suzete Ferreira, Ester Sabino
Virology Journal , 2009, DOI: 10.1186/1743-422x-6-78
Abstract: A total of 36 samples were selected from 888 adult patients residing in S?o Paulo who had previously been diagnosed in two independent studies in our laboratory as being infected with subclade F1 based on pol subgenomic fragment sequencing. Proviral DNA was amplified from the purified genomic DNA of all 36 blood samples by 5 fragments overlapping PCR followed by direct sequencing. Sequence data were obtained from the 5 fragments of pure subclade F1 and phylogenetic trees were constructed and compared with previously published sequences. Subclades F1 that exhibited mosaic structure with other subtypes were omitted from any further analysisOur methods of fragment amplification and sequencing confirmed that only 5 sequences inferred from pol region as subclade F1 also holds true for the genome as a whole and, thus, estimated the true prevalence at 0.56%. The results also showed a single phylogenetic cluster of the Brazilian subclade F1 along with non-Brazilian South American isolates in both subgenomic and the full-length genomes analysis with an overall intrasubtype nucleotide divergence of 6.9%. The nucleotide differences within the South American and Central African F1 strains, in the C2-C3 env, were 8.5% and 12.3%, respectively.All together, our findings showed a surprisingly low prevalence rate of subclade F1 in Brazil and suggest that these isolates originated in Central Africa and subsequently introduced to South America.Genetic variability is a major feature of the HIV-1 and considered the key factor to frustrate efforts to halt the virus epidemic. High mutation and replication rates, genomic recombination, therapy and immune-mediated selective pressures are some of the influential forces in the evolution of HIV [1-6]. Approaching this diversity is critical to lay the groundwork for the design of successful drugs or vaccine [7].Based on the HIV-1 genetic variations and pattern observed in phylogenetic reconstruction, researchers have classified the virus into g
Distribution of hepatitis B virus genotypes and viral load levels in Brazilian chronically infected patients in S?o Paulo city
Alcalde, Rosana;Melo, Fernando Lucas;Nishiya, Anna;Ferreira, Suzete Cleusa;Langhi Júnior, Mario Dante;Fernandes, Simone Sena;Marcondes, Luis Augusto;Duarte, Alberto José Silva;Casseb, Jorge;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2009, DOI: 10.1590/S0036-46652009000500006
Abstract: the objective of the present study was to evaluate the serum viral load in chronically infected hepatitis b virus (hbv) patients and to investigate the distribution of hbv genotypes in s?o paulo city. quantitative hbv-dna assays and hbv genotyping have gained importance for predicting hbv disease progression, have been employed for assessing infectivity, for treatment monitoring and for detecting the emergence of drug resistance. twenty-nine brazilian patients with suspected chronic hepatitis b were studied, using real time pcr for viral load determination and direct dna sequencing for the genotyping. the serology revealed chronic hbv infection in 22 samples. the hbv-dna was positive in 68% samples (15/22). the phylogenetic analysis disclosed that eleven patients were infected with hbv genotype a, two with genotype f and two with genotype d. thus, the genotype a was the most prevalent in our study.
High Human Herpesvirus 8 (HHV-8) Prevalence, Clinical Correlates and High Incidence among Recently HIV-1-Infected Subjects in Sao Paulo, Brazil
Mariana Dias Batista, Suzete Ferreira, Mariana M. Sauer, Helena Tomiyama, Maria Teresa Maidana Giret, Cláudio S. Pannuti, Ricardo S. Diaz, Ester C. Sabino, Esper G. Kallas
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0005613
Abstract: Background Human herpesvirus 8 (HHV-8) is the etiological agent for Kaposi Sarcoma, which occurs especially in HIV-infected subjects. HHV-8 infection and its clinical correlates have not been well characterized in recently HIV-1-infected subjects, especially men who have sex with men (MSM). Methodology/ Principal Findings We assessed the HHV-8 seroprevalence, clinical correlates, and incidence after one year of follow-up in a cohort of 228 recently HIV-1-infected individuals, of whom 83.6% were MSM, using indirect immunofluorescence assay. The prevalence of HHV-8 infection at the time of cohort enrollment was 25.9% (59/228). In the univariate model, there were significant associations with male gender, black ethnicity, MSM practice, and previous hepatitis B virus and syphilis infections. In the multivariate model we could still demonstrate association with MSM, hepatitis B, and black ethnicity. No differences in mean CD4+ cell counts or HIV viral load according to HHV-8 status were found. In terms of incidence, there were 23/127 (18.1%) seroconversions in the cohort after 1 year. Conclusions HHV-8 is highly prevalent among recently HIV-1-infected subjects. Correlations with other sexually transmitted infections suggest common transmission routes.
HCV Genotypes, Characterization of Mutations Conferring Drug Resistance to Protease Inhibitors, and Risk Factors among Blood Donors in S?o Paulo, Brazil
Anna S. Nishiya, Cesar de Almeida-Neto, Suzete C. Ferreira, Cecília S. Alencar, Claudia Di-Lorenzo-Oliveira, José E. Levi, Nanci A. Salles, Alfredo Mendrone, Ester C. Sabino
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086413
Abstract: Background Hepatitis C virus (HCV) infection is a global health problem estimated to affect almost 200 million people worldwide. The aim of this study is to analyze the subtypes and existence of variants resistant to protease inhibitors and their association with potential HCV risk factors among blood donors in Brazil. Methods Repeat anti-HCV reactive blood donors are systematically asked to return for retest, notification, and counseling in which they are interviewed for risk factors for transfusion-transmitted diseases. We analyzed 202 donors who returned for counseling from 2007 to 2010 and presented enzyme immunoassay- and immunoblot-reactive results. The HCV genotypes and resistance mutation analyses were determined by the direct sequencing of the NS5b and NS3 regions, respectively. The HCV viral load was determined using an in-house real-time PCR assay targeting the 5′-NCR. Results HCV subtypes 1b, 1a, and 3a were found in 45.5%, 32.0%, and 18.0% of the donors, respectively. The mean viral load of genotype 1 was significantly higher than that of the genotype 3 isolates. Subtype 1a was more frequent among young donors and 3a was more frequent among older donors. Protease inhibitor-resistant variants were detected in 12.8% of the sequenced samples belonging to genotype 1, and a higher frequency was observed among subtype 1a (20%) in comparison to 1b (8%). There was no difference in the prevalence of HCV risk factors among the genotypes or drug-resistant variants. Conclusions We found a predominance of subtype 1b, with an increase in the frequency of subtype 1a, in young subjects. Mutations conferring resistance to NS3 inhibitors were frequent in treatment-na?ve blood donors, particularly those infected with subtype 1a. These variants were detected in the major viral population of HCV quasispecies, have replicative capacities comparable to nonresistant strains, and could be important for predicting the response to antiviral triple therapy.
Emergence and pupal mortality factors of Anastrepha obliqua (Macq.) (Diptera: Tephritidae) along the fruiting season of the host Spondias dulcis L.
Neotropical Entomology , 2001, DOI: 10.1590/S1519-566X2001000200002
Abstract: the emergence and pupal mortality factors of anastrepha obliqua (macq.) (diptera: tephritidae) along the fruiting season of the host plant, spondias dulcis l. (anarcadiaceae), were studied in sert?ozinho, sp, brazil, under field and laboratory conditions. eclosed and uneclosed puparia were recorded in two experimental conditions. the number of emerged flies and parasitoids were determined in the eclosed puparia. the uneclosed puparia were inspected and classified into living (dormant) and dead. the following pupal mortality factors were considered: disease, desiccation, predation and parasitism. out of the total of 1,204 puparia analyzed, 53% emerged and 47% remained uneclosed. out of the uneclosed puparia, 25.3% contained dormant pupae and 21.7% showed no signs of emergence. among the dormant pupae, 17.8% were flies, 0.2% of which emerged; 7.5% were parasitoids, 4.7% of them emerged. the initial parasitism was 8.6%, increasing to 15.3% after the emergence of dormant pupae. predatory activity (natural condition), especially when time of exposure was long, and desiccation (laboratory condition) were the predominant causes of pupal mortality. variation in mortality caused by parasites and pathogens (bacteria and fungi) seems to play a minor role. control by predators on fruit fly population is significant. however, the factors, which regulate induction, maintenance and termination of dormancy, are still to be determined. an estimate of the dormancy and of the biotic and abiotic pupal mortality factors are essential to understand the adaptive strategies of a.obliqua and its parasitoids and to develop effective methods of control in tropical regions.
Monica Tavares,Suzete Venturelli
ARS (S?o Paulo) , 2005, DOI: 10.1590/s1678-53202005000100008
Cibercomunica o cíbrida no continuum virtualidade aumentada e realidade aumentada: era uma vez ... a realidade
Diana Domingues,Suzete Venturelli
ARS (S?o Paulo) , 2007, DOI: 10.1590/s1678-53202007000200011
Mario Maciel,Suzete Venturelli
Conex?o : Comunica??o e Cultura , 2004,
Abstract: A história dos games eletr nicos come ou nos anos 60 do século passado e hoje demonstra que provocaram inusitados impactos culturais. A criatividade imagética levou designers, programadores, artistas, músicos e cineastas a realizar exposi es sobre esse fen meno, por exemplo, em 2002 na Barbican Gallery de Londres, a Game On e, em 2003, no Itaú Cultural, S o Paulo, a Game o quê? Foram exposi es que marcaram momentos de reconhecimento dos jogos para computador. Mostraram que jogo eletr nico interessa a muitas tribos e a diferentes públicos onde o jogar, tanto reflete como molda culturas. Este texto busca, ent o, apresentar a história dos games a partir das pesquisas tecnológicas, cujas poéticas perpassam roteiros e narrativas interativas garantidas pelas máquinas computacionais.
Effects of Generic versus Non-Generic Feedback on Motor Learning in Children
Suzete Chiviacowsky, Ricardo Drews
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0088989
Abstract: Non-generic feedback refers to a specific event and implies that performance is malleable, while generic feedback implies that task performance reflects an inherent ability. The present study examined the influences of generic versus non-generic feedback on motor performance and learning in 10-year-old children. In the first experiment, using soccer ball kicking at a target as a task, providing participants with generic feedback resulted in worse performance than providing non-generic feedback, after both groups received negative feedback. The second experiment measured more permanent effects. Results of a retention test, performed one day after practicing a throwing task, showed that participants who received non-generic feedback during practice outperformed the generic feedback group, after receiving a negative feedback statement. The findings demonstrate the importance of the wording of feedback. Even though different positive feedback statements may not have an immediate influence on performance, they can affect performance, and presumably individuals' motivation, when performance is (purportedly) poor. Feedback implying that performance is malleable, rather than due to an inherent ability, seems to have the potential to inoculate learners against setbacks – a situation frequently encountered in the context of motor performance and learning.
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