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Search Results: 1 - 10 of 637 matches for " Superoxide dismutase "
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M Mehrabani,m Djalali,M R Sadeghi,B Hajibeigi
Acta Medica Iranica , 2008,
Abstract: "nIron is a pro-oxidant cofactor that may be linked to atherosclerosis progression. Free iron catalyzes the generation of free radicals and free radicals promote the oxidation of lipids. Reduction of body iron stores secondary to blood donation has been hypothesized to reduce lipid peroxidation. The aim of this study was to evaluate the association between blood donation and antioxidant enzymes and lipid peroxidation product malondialdehyde (MDA). We investigated hemoglobin (Hb), serum ferritin, MDA level, glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities in the whole blood of 150 male volunteer blood donors aged from 30 to 60 years attending Tehran Blood Transfusion Center. Subjects were divided into 5 groups according to the frequency of blood donation per year. With increasing the number of blood donation in a year, the body iron stores, GPX activities and serum MDA were significantly reduced (P < 0.05) but SOD was significantly increased (P < 0.05). High-frequency blood donors had evidence of decreased body iron stores, decreased lipid peroxidation and enhanced activity of antioxidant enzymes when compared with low-frequency donors
Superoxide Dismutase: Therapeutic Targets in SOD Related Pathology  [PDF]
Filip Cristiana, Albu Elena, Zamosteanu Nina
Health (Health) , 2014, DOI: 10.4236/health.2014.610123
Abstract: There are growing evidences on the role of adaptive mechanisms of all cell types in pathological processes: atherosclerosis, ischemic attack, bacterial infections, etc. All kinds of these processes involve as main mechanism oxidative stress. Aerobic organisms use oxygen in processes that accidentally or deliberately generate aggressive species for the biologic components in the form of radicals. Radicals were looked initially as “harmful” molecules and this is true for large quantities but in small or even moderate amounts these molecules prove to have a physiological role. Reactive species are highly reactive and as a consequence are short living species. Their impact is supposed to be limited in the proximity area of their formation. Instead recent evidences indicate their implications in cellular signaling suggesting that individual chemical properties of reactive species make a difference in their biological role. This paper presents superoxide, nitric oxide and peroxide radical generation under cellular changing conditions, the adapting behavior of the enzymes that synthesize and remove them as well as some therapeutic target in superoxide related pathology.
GroESL protects superoxide dismutase (SOD)— Deficient cells against oxidative stress and is a chaperone for SOD  [PDF]
Gary J. Hunter, Thérèse Hunter
Health (Health) , 2013, DOI: 10.4236/health.2013.510232

Superoxide dismutase (SOD)-deficient Escherichia coli OX326Acells are protected against chemically-induced oxidative stress by expression of the chaperonin GroESL. This protection is equivalent to expression of superoxide dismutase even though GroESL has no inherent SOD activity. Co-overexpression of GroESL and SOD in the same cells results in higher protein yields of SOD and greater metallation of SOD when compared with expression of SOD alone. Greater metallation results in the higher specific activity of SOD that is observed in heat shock, and is not due to increased synthesis of SOD mRNA or protein.

Modifying Polyacrylamide Background Color for the Nitroblue Tetrazolium-Based Superoxide Dismutase Staining Assay  [PDF]
Robert Louis Bertrand, Michael Okechukwu Eze
Advances in Enzyme Research (AER) , 2014, DOI: 10.4236/aer.2014.22008
Abstract: The reduction of nitroblue tetrazolium by superoxide radicals generated from photo-reactive riboflavin has been in use for more than four decades to detect superoxide dismutase (SOD) on nondenaturing polyacrylamide gels. SOD research in medicine and biochemistry has warranted the development of multiple assay variants to overcome specific experimental constraints or to combine the SOD assay with other enzyme assays. Fine-tuning reagent concentrations to effectively visualize bands continue to be a major research obstacle in assay development. Herein we describe a straightforward technique to reliably adjust the background color of polyacrylamide gels without compromising assay efficacy. Low micromolar to low millimolar concentrations of yellow riboflavin can be mixed with the blue of reduced nitroblue tetrazolium to controllably produce blue, purple, yellow-brown, or yellow gel backgrounds. The advantage of this technique is that the assay is not modified by the introduction of new reagents. Quantitative reliability of these alternative stains was assessed by plotting determined band intensity values against known enzyme loads. The correlation (R2) values of trial averages were compared against the average correlation of the standard 0.028 mM riboflavin solution using pooled standard deviation and Student’s T-test at 95% confidence. Assay sensitivity was assessed by comparing lowest possible visible enzyme load of the experimental stains with the 0.028 mM riboflavin standard. No difference in the quantitative reliability was found in any riboflavin concentration. The minimum reliable sensitivity of the assay was found to be 10 ng for each concentration of riboflavin. This technique has already been employed to analyze SOD protein expression levels in extracts of Escherichia coli (Bertrand et al., Med Hypotheses 2012; 78:130-133, 2012; Bertrand & Eze, Adv. Enz. Res., 1: 132-141, 2013).
Lipid Peroxidation and Some Antioxidant Enzymes Evaluation in Apple Cider Vinegar (ACV) Treated Male and Female Wistar Rats Exposed to Chronic Restraint Stress  [PDF]
R. A. Abdulrauf, F. A. Dawud, N. S. Emmanuel, H. D. Muhammad, A. S. Dange, B. A. David, A. E. Ogweje, A. U. Alexander, M. Yahuza
Advances in Enzyme Research (AER) , 2018, DOI: 10.4236/aer.2018.63003
Abstract: This study was designed to assess the effect to apple cider vinegar (ACV) on oxidative stress biomarkers in male and female Wistar rats exposed to chronic restraint stress. Severe and persistent stress elevates reactive oxygen species (ROS) production by metabolic and physiological processes; causing cellular damage. Thirty (30) Adult Wistar rats of both sexes weighing about 150 - 200 g were divided into 3 groups each consisting of a male and female subgroup and given the following treatments once a day for 21 days: Normal control group received 0.5 ml distilled water orally, the restraint stress (RS) group was exposed to chronic restraint stress 6 hours daily while the Apple cider vinegar (ACV)-treated group received 4 ml/kg of apple cider vinegar orally in addition to chronic restraint stress 6 hours daily. The rats were sacrificed after the experimental period and blood was collected via cardiac puncture for assessing oxidative stress biomarkers. ACV (4 ml/kg) treatment decreased lipid peroxidation (MDA) and serum catalase (CAT) activity while upregulating endogenous superoxide dismutase (SOD) activity. The findings of this study show that the female Wistar rats are more predisposed to the antioxidant effect of ACV than the males.
Evaluación de la toxicidad de cobre en suelos a través de biomarcadores de estrés oxidativo en eisenia foetida
Gaete, Hernán;Hidalgo, María Eliana;Neaman, Alexander;ávila, Gonzalo;
Química Nova , 2010, DOI: 10.1590/S0100-40422010000300014
Abstract: copper toxicity in soil was evaluated using biomarkers of oxidative stress (catalase enzyme activity, superoxide dismutase and lipid peroxidation) in the earthworm eisenia foetida. agricultural topsoils from mining areas of the aconcagua river basin were collected. total copper concentrations were in the range of 94-959 mg kg-1, while the exchangeable copper concentrations were in the range of 46-2225 μg kg-1. earthworms exposed to soil with exchangeable copper concentrations above 32 μg kg-1 showed an increase in catalase activity. parameters of antioxidant activity were more sensitive than the weight change and thus can be used as appropriate biomarkers in eisenia foetida.
Docking Studies of Thiophene And Superoxide Dismutase.
Anisha Brigit Shajan*
Biomirror , 2011,
Abstract: Thiophene is a heterocyclic compound which has both pharmaceutical and industrial uses. It is also known to have larvicidal activities. The compound is naturally isolated by tissue culture from T.patula. It showed high larvicidal activity on mosquito larvae. Further by docking studies carried out on thiophene and superoxide dismutase by using biological databases like NCBI, PDB and PubChem and software like hex and spdbv the action of thiophene on mosquito larvae was further confirmed.
Relationship of Uric Acid with Superoxide Dismutase (Sod) in Induced Hyperuricemic Rat Model  [PDF]
Shiza Batool, Iftikhar Ahmed, Muhammad Sarwar, Hafeez ul Hassan
Pharmacology & Pharmacy (PP) , 2012, DOI: 10.4236/pp.2012.34054
Abstract: Increase uric acid levels have been found in oxidative stress. Urate radicals do not react with oxygen to form another peroxy radical, thus increasing the efficacy of uric acid as an antioxidant. Therefore, this study is designed to measure the level of uric acids and find out the relationship of uric acid with superoxide dismutase in induced hyperuricemic model. Forty male albino rats with an average weight of 180 ± 2 g were selected. The rats were grouped. The animals were fed on standard diet and given tap water ad libitum until treatment. Albino rats were divided into four groups. Group A(10)-control given only standard diet, group B(10) fed on 60% fructose with standard diet , group C(10) fed on fructose, standard diet and intraperitonially oxonic acid 250 mg/kg and group D (10) only on injection intraperotonially oxonic acid 250 mg/kg. At the end of study 10 mL of blood was drawn from heart of rats. Then blood was estimated for superoxide dismutase and uric acids done by kit methods randox-manual/Rx monza UA230/UA 233. Results: In Group C superoxide dismutase was found to be 32 % (244 mg/dL ± 2.23) more than control. In the same group the uric acid concentration was highly significantly correlated with control. Conclusion: The uric acid concentration increases when we take fructose up to 60% in our diet. It also increases superoxide dismutase concentration. More than this value may have inverse effect on the uric acid level and its role as an antioxidant may become inversed.
Glutathione peroxidase, superoxide dismutase and catalase activities in children with chronic hepatitis  [PDF]
Nagwa Abdallah Ismail, Sawsan H. Okasha, Anil Dhawan, Azza M. O. Abdel Rahman, Nehal Abdel Hamid, Olfat Shaker
Advances in Bioscience and Biotechnology (ABB) , 2012, DOI: 10.4236/abb.2012.327119
Abstract: The advantages of measuring hepatic oxidative status in liver biopsy are that it helps in diagnosis of hepatic dysfunction, reflects the degree of deterioration in the liver tissues, and helps to determine the severity of hepatic injury. We aimed to study the oxidative stress state in children with chronic hepatitis by using indirect approach in which antioxidant enzymes such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT) are determined in the liver tissue. The present study included 21 children and adolescents (12 males, 9 females) suffering from chronic hepatitis. Patients were selected from the Hepatology Clinic, New Children’s Hospital, Cairo University from November 2006 till 2009 and compared with a group of 7 children who happened to have incidental normal liver biopsy. Children with chronic hepatitis had mean age 8.12 ± 1.15 years. It was further subdivided into 2 subgroups: chronic viral heaptitis (n = 13) and cryptogenic hepatitis (n = 8). GPX, SOD and CAT levels were measured in fresh liver tissue (cell free homogenates) using ELISA. In chronic hepatitis group; there was a significant increase in the hepatic GPX activity (38.59 ± 35.82 nmol/min/ml) as compared to the control group (10.62 ± 6.68 nmol/min/ml). Also a significant correlation was observed between SOD and both ALT (r = 0.87, p < 0.05) and AST (r = 0.74, p < 0.05). GPX correlated with ALT (r = 0.80, p < 0.05) level in the chronic viral hepatitis subgroup. Our findings suggest that oxidative stress could play a role in the pathogenesis of chronic hepatitis. These preliminary results are encouraging to conduct more extensive clinical studies combining antioxidant therapy with various treatments.
Spin-Labelled 1-Ethyl-1-Nitrosourea Prevents Doxorubicin and Bleomycin-Induced Oxidative Stress in Lungs, Hearts and Kidneys of Tumour-Bearing Mice  [PDF]
Veselina G. Gadjeva, Galina D. Nikolova, Boncho G. Grigorov, Antoaneta M. Zheleva, Anna N. Tolekova, Maya I. Vasileva
Advances in Biological Chemistry (ABC) , 2014, DOI: 10.4236/abc.2014.45035
Abstract: This study was carried out to determine the possible protective effect of 1-ethyl-3-[4-(2, 2, 6, 6-tetramethylpiperidine-1-oxyl)]-1-nitrosourea (SLENU), recently synthesised in our laboratory on doxorubicin and bleomycin-induced oxidative toxicity in C57 black tumour-bearing mice. Specifically, alterations in some biomarkers of oxidative stress, such as lipid peroxidation products measured as malondialdehyde (MDA) levels and activities of the antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were studied in lung, heart and kidney homogenates isolated from C57 black tumor-bearing mice after i.p. treatment with solutions of DOX (60 mg/kg) and BLM (60 mg/kg). The same biomarkers were also measured after i.p. pretreatment of mice with SLENU (100 mg/kg). After treatment with doxorubicin, heart and kidney homogenates of mice had significantly higher productions of lipid peroxidation compared to lung homogenates. It was accompanied by increased activity of the antioxidant defence enzyme superoxide dismutase and decreased activity of catalase. Bleomycin-induced oxidative stress was confirmed by significantly higher production of lipid peroxidation in lungs compared to heart homogenates, elevation of the anti-oxidant activity of superoxide dismutase and decreased activity of catalase enzymes. After pre-treatment of the mice with SLENU, the levels of all studied oxidative stress biomarkers were significantly improved in comparison with those of the mice treated alone with either bleomycin, or doxorubicin. The present results and those from a previously demonstrated superoxide scavenging activities (SSA) of the nitrosourea SLENU have enabled us to explain the protective effect of the spin-labelled nitrosourea on doxorubicin and bleomycin-induced oxidative stress by scavenging of  O2- and increased NO release.
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