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Search Results: 1 - 10 of 201730 matches for " Suely P Palomino "
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Involvement of TLR2 and TLR4, Chlamydophila pneumoniae and Mycoplasma pneumoniae in adventitial inflammation of aortic atherosclerotic aneurysm  [PDF]
Renata Melo de Assis, Maria de Lourdes Higuchi, Marcia Martins Reis, Suely A. P. Palomino, Rosario Dominguez Crespo Hirata, Mario Hiroyuki Hirata
World Journal of Cardiovascular Diseases (WJCD) , 2014, DOI: 10.4236/wjcd.2014.41004
Abstract:

Aortic atherosclerotic aneurysm (AAA) is associated with adventitial inflammation where infection is suggested to have a role. Co-infection with Chlamydophila pneumoniae (Cp) and Mycoplasma pneumoniae (Mp) was linked with coronary plaque rupture, in association with vessel dilatation and adventitial inflammation. Pathogens are recognized by Toll-like receptors (TLRs) development of the inflammatory process. Objective: Here, we studied whether co-infection by Cp and Mp was involved in the increased inflammation present in AAA and if it could be associated with deficient expression of TLRs. We compared human samples of AAA with non-dilated human aortic atherosclerotic lesions, regarding the amount of Cp and Mp antigens, and expression of TLR2 and TLR4. Methods: Two groups of aorta fragments were analyzed: G1 (n = 13) moderate atherosclerosis and G2 (n = 14) AAA samples, through immunohisto-chemistry and in situ hybridization methods. Results: Mp and Cp antigens in intima/medial layer were greater in G2 than G1, with no difference in adventitia. TLR2 and TLR4 were higher in G2 than G1 adventitia fat. There was a correlation between Mp versus TLR2 and of TLR4 in intima/medial layer and in adventitia of G1, but there was a lack of correlation in G2. In Cp adventitia, the correlation in G1 was high with TLR2 but not with TLR4, and in G2 the correlation was positive for both TLRs. Conclusion: This study favors the concept that symbiotic co-infection by Cp and Mp participates in the pathogenesis of AAA. It also emphasizes that adventitial fat is the initial site for colonization of these bacteria that probably reach

Oral PTCTS (Particulated Transialidase) Removes Serum Microparticles and Decreases Inflammation in Atherosclerotic Plaques of Rabbits  [PDF]
Shérrira M. Garavelo, Jaqueline J. Pereira, Nilsa S. Y. Wadt, Marcia M. Reis, Renata N. Ikegami, Joyce T. Kawakami, Abdelali Agouni, Suely A. P. Palomino, Dulcinéia Abdalla, Maria de Lourdes Higuchi
Advances in Nanoparticles (ANP) , 2015, DOI: 10.4236/anp.2015.44012
Abstract: Background: Previous studies showed that atherosclerotic plaque vulnerability was related with microparticles (MPs)-vesicles larger than 100 nm, which released MMP9 collagenase. In our previous study, intramuscular injection of a new drug (PTCTS) normalized oxidized LDL serum levels and reduced rabbit atherosclerosis. Now, we studied administration of oral PTCTS in order to clarify anti-atherosclerotic mechanism of action, analyzing if the treatment removed MPs containing ox-LDL and Mycoplasma pneumoniae antigens and improved the immune response. Methods: We compared two groups of rabbits. Control group (CG, n = 6)—1% cholesterol enriched diet for 12 weeks; Treated group (TG, n = 8)—1% cholesterol enriched diet for 12 weeks with administration of PTCTS (400 μl/day) during the last 6 weeks of diet. The animals had their blood collected, in three different phases of the protocol before being fed with hypercholesterolemic diet, before being treated with water or PTCTS and at the moment of sacrifice. The serum was submitted to immunofluorescence technique to evaluate the quantity of microparticles marked with antibodies against Mycoplasma pneumoniae and ox-LDL. A fragment of aorta was submitted to immunohistochemical detection of antigens from MMP9, ox-LDL, NF-κB and IL-1β. Results: PTCTS showed significant reduction in MMP-9 (P = 0.001) and a tendency of reducing IL-1β (P = 0.09) in the aortic plaques compared with CG. In the serum, PTCTS was able to remove microparticles containing antigen of ox-LDL (P = 0.004) and Mycoplasma pneumoniae (P < 0.001). Conclusion: Oral treatment with PTCTS presented more adequate inflammatory response by reducing levels of ox-LDL, IL-1β and mycoplasma, as well as a better stabilization of the atheromatous plaque by reducing levels of MMP-9, avoiding plaque rupture, without causing mortality or toxicity.
Mycoplasma pneumoniae and/or Chlamydophila pneumoniae inoculation causing different aggravations in cholesterol-induced atherosclerosis in apoE KO male mice
Sueli B Damy, Maria L Higuchi, Jorge Timenetsky, Márcia M Reis, Suely P Palomino, Renata N Ikegami, Fabiana P Santos, Junko T Osaka, Luiz P Figueiredo
BMC Microbiology , 2009, DOI: 10.1186/1471-2180-9-194
Abstract: The mean and standard deviation of plaque height, % luminal obstruction, external diameter, the plaque area/intimal surface ratio and the adventitial inflammation values are the following for each group: MP (0.20 +/- 0.12 mm, 69 +/- 26%, 0.38 +/- 0.11 mm, 0.04 +/- 0.04 and 0.22 +/- 0.67), CP (0.23 +/- 0.08 mm, 90 +/- 26%, 0.37 +/- 0.08 mm, 0.04 +/- 0.03, and 0.44 +/- 0.53), MP + CP (18 +/- 0.08 mm, 84 +/- 4.0%, 0.35 +/- 0.25 mm, 0.03 +/- 0.03 and 1.33 +/- 0.82) and sham (0.08 +/- 0.09 mm, 42 +/- 46%, 0.30 +/- 0.10 mm, 0.02 +/- 0.03 and 0.71 ± 0.76). A wider area of plaque/intimal surface was observed in MP + CP inoculated groups (p = 0.07 and 0.06) as well as an increased plaque height in CP (p = 0.01) in comparison with sham group. There was also an increased luminal obstruction (p = 0.047) in CP inoculated group in comparison to sham group. Adventitial inflammation in MP + CP inoculated group was higher than MP, CP and the sham groups (p = 0.02).Inoculation of CP, MP or both agents in C57BL/6 apoE KO male mice caused aggravation of experimental atherosclerosis induced by cholesterol-enriched diet, with distinct characteristics. CP inoculation increased the plaque height with positive vessel remodeling and co-inoculation of MP + CP caused the highest adventitial inflammation measures.Atherosclerosis is considered an arterial inflammatory disease resulting from lipid entrance in the vascular wall and subsequent oxidation. Lipid oxidation has been related to infectious agents [1], mainly Chlamydophila or Chlamydia pneumoniae (CP) [2-4]. CP induced or accelerated atherosclerosis in experimental animals [5-7]. Although more than 700 studies have been published focusing CP in atherosclerosis, the inconsistent results of clinical trials using antibiotic therapy discouraged the infection theory. However, our previous studies have shown that co-infection of CP and Mycoplasma pneumoniae (MP) is usually present in atherosclerotic plaques, in greater amount in ruptured plaque
Comparison between Adventitial and Intimal Inflammation of Ruptured and Nonruptured Atherosclerotic Plaques in Human Coronary Arteries
Higuchi Maria L.,Gutierrez Paulo S.,Bezerra Hiram G.,Palomino Suely A.
Arquivos Brasileiros de Cardiologia , 2002,
Abstract: OBJECTIVE: To verify the possible role of adventitial inflammation in atherosclerotic plaque vulnerability and coronary artery remodelling. METHODS: We compared the mean numbers of lymphocytes in the adventitia and in the plaque of ruptured thrombosed and stable equi-stenotic coronary segments of 34 patients who died due to acute myocardial infarction. We also analysed adventitial microvessels, adventitial fibrosis and the external elastic membrane. RESULTS: In the adventitia, the numbers of lymphocytes and microvessels/mm2 were 69.5±88.3 and 60.9± 32.1 in culprit lesions and 16.4 ± 21.1 and 44.3±16.1 in stable lesions (p<0.05); within the plaques, the mean number of lymphocytes was 24±40.8 in culprit lesions and 10.9±13.2 in stable ones (p=0.17). The mean percent area of adventitial fibrosis/cross-sectional area of the vessel was significantly lower in unstable plaques (p<0.001). The confocal images showed holes in the external elastic membrane. CONCLUSION: Unstable plaques exhibit chronic pan-arteritis, accompanied by enlargement, medial thinning, and less fibrosis than in stable lesions, which is compatible with vessel aneurysm. Adventitial inflammation may contribute significantly to atheroma instability.
Coinfection with Mycoplasma Pneumoniae and Chlamydia Pneumoniae in ruptured plaques associated with acute myocardial infarction
Higuchi Maria de Lourdes,Reis Marcia Martins,Sambiase Nádia Vieira,Palomino Suely Aparecida Pinheiro
Arquivos Brasileiros de Cardiologia , 2003,
Abstract: OBJECTIVE: To study atheromas, Mycoplasma pneumoniae (M. pneumoniae), and Chlamydia pneumoniae (C. pneumoniae). METHODS: C. pneumoniae was studied with immunohistochemistry and M. pneumoniae with in situ hybridization (ISH), in segments of coronary arteries (SCA) as follows: group A - thrombosed ruptured plaques (TRP) of 23 patients who died due to acute myocardial infarction (AMI); group B - 23 nonruptured plaques (NRP) of group A patients; group C - NRP of 11 coronary patients who did not die due to AMI; and group D - 11 SCA from patients with dilated cardiomyopathy or Chagas' disease without atherosclerosis. RESULTS: The mean number of C. pneumoniae+ cells/400x in groups A, B, C, and D was, respectively, 3.3±3.6; 1.0±1.3; 1.2±2.4; and 0.4±0.3; and the percentage of M. pneumoniae area was, respectively, 3.9±3.5; 1.5± 1.6; 0.9±0.9; and 0.4±0.2. More M. pneumoniae and C. pneumoniae were found in of group A than in group B (P<0.01). Good correlation was seen between the area of the vessel and the M. pneumoniae area in the plaque (r = 0.46; P=0.001) and between C. pneumoniae+ cells and CD4+ T lymphocytes (r = 0.42; P<0.01). The number of C. pneumoniae+ cells correlated with CD20+ B cells (r=0.48; P<0.01). CONCLUSION: M. pneumoniae and C. pneumoniae are more frequently found in TRP correlate with the intensity of the inflammation and diameter of the vessel (positive remodeling).
Identification and Recovery of Volatiles Organic Compounds (VOCs) in the Coffee-Producing Wastewater  [PDF]
Alcilucia Oliveira, Lourdes M. C. Cabral, Humberto Bizzo, Neusa P. Arruda, Suely P. Freitas
Journal of Water Resource and Protection (JWARP) , 2014, DOI: 10.4236/jwarp.2014.64039
Abstract:

The objectives of the present study were to recover and identify volatiles organic compounds (VOCs) in the wastewater (WW) coming from a soluble coffee industrial plant. For VOCs recover, the WW was concentrated by pervaporation using a polydimethylsiloxane (PDMS) membrane. Furthermore, the solid phase micro extraction (SPME) and gas chromatography-mass spectrometry (GC-MS) were used for VOCs identification. The multivariate method of principal component analysis (PCA) was performed to evaluate differences amongst the feed and concentrate streams. Regarding the number of VOCs identified in the wastewater (33) and permeate (72), a significant statistical difference at p < 0.05 was observed. Clusters discriminating were clearly observed, as the feed and permeate streams are compared. Thus, we can conclude that pervaporation process using a PDMS membrane was effective to recover different class of VOCs present in the aqueous effluent and can be recommended for industrial wastewater treatment.

Alta prevalencia de trastorno específico de lenguaje en isla Robinson Crusoe y probable efecto fundador
Villanueva,Pía; de Barbieri,Zulema; Palomino,Hernán M; Palomino,Hernán;
Revista médica de Chile , 2008, DOI: 10.4067/S0034-98872008000200007
Abstract: specific language impairment (sli) occurs in 2% to 8% of preschool children. major and candidate genes are probably involved. genetic drift is a cause for the presence of high frequencies of deleterious alíeles of a specific disease and the founder effect is one of its forms. robinson crusoe island has 633 inhabitants and its actual population began with 8 families that repopulated the island at the end ofxixth century. aim to assess the frequency of specific language impairment among children living in robinson crusoe island. material and methods: all 66 children aged between 3 and 9 years living in the island, were studied. parents were interviewed and in children, non verbal intelligence, audiometric parameters, comprehension and expression of oral language were assessed. extended genealogies were also performed. results: forty children had at least one parent that was descending of founder families. among these, 35% had sli. eighth five percent of sli affected children came from the same colonizer family. conclusions: the prevalence of sli in robinson crusoe island is higher than that reponed in mainland chile and abroad. this high prevalence, associated to a high frequency of consanguinity, supports the influence of genetic mechanisms in sli transmission, based on a founder effect
Alta prevalencia de trastorno específico de lenguaje en isla Robinson Crusoe y probable efecto fundador High prevalence of specific language impairment in Robinson Crusoe Island. A possible founder effect
Pía Villanueva,Zulema de Barbieri,Hernán M Palomino,Hernán Palomino
Revista médica de Chile , 2008,
Abstract: Specific language impairment (SLI) occurs in 2% to 8% of preschool children. Major and candidate genes are probably involved. Genetic drift is a cause for the presence of high frequencies of deleterious alíeles of a specific disease and the founder effect is one of its forms. Robinson Crusoe Island has 633 inhabitants and its actual population began with 8 families that repopulated the island at the end ofXIXth century. Aim To assess the frequency of specific language impairment among children living in Robinson Crusoe Island. Material and methods: All 66 children aged between 3 and 9 years living in the island, were studied. Parents were interviewed and in children, non verbal intelligence, audiometric parameters, comprehension and expression of oral language were assessed. Extended genealogies were also performed. Results: Forty children had at least one parent that was descending of founder families. Among these, 35% had SLI. Eighth five percent of SLI affected children came from the same colonizer family. Conclusions: The prevalence of SLI in Robinson Crusoe Island is higher than that reponed in mainland Chile and abroad. This high prevalence, associated to a high frequency of consanguinity, supports the influence of genetic mechanisms in SLI transmission, based on a founder effect
Detection of Mycoplasma pneumoniae and Chlamydia pneumoniae in ruptured atherosclerotic plaques
Higuchi M.L.,Sambiase N.,Palomino S.,Gutierrez P.
Brazilian Journal of Medical and Biological Research , 2000,
Abstract: This paper reports what is apparently the first observation of Mycoplasma pneumoniae in association with Chlamydia pneumoniae in thrombosed ruptured atheromas. We performed electron microscopy and in situ hybridization in specimens from three patients who died of acute myocardial infarction. These patients had typical symptoms of acute ischemic syndrome. Mycoplasmas were present mainly in the lipid core of the ruptured thrombosed plaque. Vulnerable atheromas are rich in cholesterol and may favor the growth of mycoplasmas, the only microorganisms that require cholesterol for survival. We suggest that the association of Mycoplasma pneumoniae and Chlamydia pneumoniae may increase the virulence of these microorganisms, favoring proliferation, plaque inflammation and possibly plaque rupture.
Comparison between Adventitial and Intimal Inflammation of Ruptured and Nonruptured Atherosclerotic Plaques in Human Coronary Arteries
Higuchi, Maria L.;Gutierrez, Paulo S.;Bezerra, Hiram G.;Palomino, Suely A.;Aiello, Vera D.;Silvestre, Júlia M. L.;Libby, Peter;Ramires, José A. F.;
Arquivos Brasileiros de Cardiologia , 2002, DOI: 10.1590/S0066-782X2002001000003
Abstract: objective: to verify the possible role of adventitial inflammation in atherosclerotic plaque vulnerability and coronary artery remodelling. methods: we compared the mean numbers of lymphocytes in the adventitia and in the plaque of ruptured thrombosed and stable equi-stenotic coronary segments of 34 patients who died due to acute myocardial infarction. we also analysed adventitial microvessels, adventitial fibrosis and the external elastic membrane. results: in the adventitia, the numbers of lymphocytes and microvessels/mm2 were 69.5±88.3 and 60.9± 32.1 in culprit lesions and 16.4 ± 21.1 and 44.3±16.1 in stable lesions (p<0.05); within the plaques, the mean number of lymphocytes was 24±40.8 in culprit lesions and 10.9±13.2 in stable ones (p=0.17). the mean percent area of adventitial fibrosis/cross-sectional area of the vessel was significantly lower in unstable plaques (p<0.001). the confocal images showed holes in the external elastic membrane. conclusion: unstable plaques exhibit chronic pan-arteritis, accompanied by enlargement, medial thinning, and less fibrosis than in stable lesions, which is compatible with vessel aneurysm. adventitial inflammation may contribute significantly to atheroma instability.
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