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Search Results: 1 - 10 of 153 matches for " Subbarao Indugula "
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Quality assessment of buccal versus blood genomic DNA using the Affymetrix 500 K GeneChip
Jessica G Woo, Guangyun Sun, Mary Haverbusch, Subbarao Indugula, Lisa J Martin, Joseph P Broderick, Ranjan Deka, Daniel Woo
BMC Genetics , 2007, DOI: 10.1186/1471-2156-8-79
Abstract: Buccal cytobrushes stored for ~7 years at -80°C prior to extraction yielded sufficient double stranded DNA (dsDNA) to be successfully genotyped on the Affymetrix ~262 K NspI chip, with yields between 536 and 1047 ng dsDNA. Using the BRLMM algorithm, genotyping call rates for blood samples averaged 98.4%, and for buccal samples averaged 97.8%. Matched blood samples exhibited 99.2% concordance, while matched blood and buccal samples exhibited 98.8% concordance.Buccal cytobrushes stored long-term result in sufficient dsDNA concentrations to achieve high genotyping call rates and concordance with stored blood samples in the context of Affymetrix 500 K SNP genotyping. Thus, given high-quality collection and storage protocols, it is possible to use stored buccal cytobrush samples for genome-wide association studies.While blood is considered the optimal source for DNA, inclusion of a blood draw may deter study participation [1]. Buccal cytobrush collection is a simple, painless procedure that allows for effective DNA sampling from a large population, and has been used in several large epidemiologic studies [2,3]. However, concerns regarding the use of buccal brushes have included the lower quantity of genomic DNA isolated [4], lower quality of DNA [4,5], and the fidelity of results from buccal brushes compared with blood samples [5-7]. In addition, there is a concern that older buccal brush samples may not yield as high-quality results as fresh samples [8].The advent of large scale genotyping platforms has also resulted in a reduction in the amount of DNA required. The Affymetrix 500 K GeneChip requires only 250 ng of total genomic DNA per chip, 500 ng total, and this DNA quantity has not changed with the recent release of the Affymetrix 5.0 and 6.0 chips, which enable genotyping up to 1.8 million genetic markers [9-11]. Thus, the DNA requirements of the Affymetrix chips are well below the expected yield of total DNA for buccal samples. As the Affymetrix system uses restri
P53 pseudogene: potential role in heat shock induced apoptosis in a rat histiocytoma  [PDF]
Amere Subbarao Sreedhar
Health (Health) , 2010, DOI: 10.4236/health.2010.29156
Abstract: The p53 tumor suppressor gene is either non- functional or highly and frequently mutated in majority of cancers. In our study towards understanding cellular adaptations to stress using a rat histiocytic tumor model, we have identified mis-sense mutation in p53 that led to premature termination of translation at the carboxyl-termi- nus. Further, the cDNA isolated from heat stre- ssed cells producing two amplicons with cDNA specific primers (N-terminus) suggested occurrence of possible pseudogene(s). A comparative analysis between different tumor cell lines of rat origin and rat genomic DNA using p53 gene specific primers resulted in the amplification of a processed pseudogene and its positive interaction with wild type p53 probe on Southern blot analysis. The genomic DNA sequence analysis, and sequence comparison with cDNA discovered that the processed pseudogene lacks DNA binding domain and nuclear localization signal, however, contains the ribosomal entry and stop signals. Rat genome BLAST analysis of the pesudogene suggested chromosome-18 localization which was in addition to 14, 13, 10, 9 localization of the cDNA. In the interest of unraveling hidden dimensions of p53 tumor suppressor gene, our study explores the probability of p53 functional pseudogenes in rat histiocytoma.
Single Crystal X-Ray Diffraction Studies on Magnetic Yb5Co4Ge10  [PDF]
Udumula Subbarao, Sebastian C. Peter
Advances in Materials Physics and Chemistry (AMPC) , 2013, DOI: 10.4236/ampc.2013.31A008

The high quality single crystals of Yb5Co4Ge10 have been grown by the indium metal flux method and characterized by means of single crystal X-ray diffraction data. Yb5Co4Ge10 crystallizes in the Sc5Co4Si10 structure type, tetragonal space group P4/mbm and lattice constants are a = b = 12.6369(18) ? and c = 4.1378(8) ?. Crystal structure of Yb5Co4Ge10 composed of three-dimensional [Co4Ge12

Journal of Engineering Science and Technology , 2008,
Abstract: Gas to particle mass transfer coefficient in risers was observed to increase with gas velocity, decrease with solid circulation rate and decrease with particle size. A model to explain gas to particle mass transfer in risers is developed considering that particles move as clusters. The model equations compare well with the available experimental observations on gas to particle mass transfer coefficients in risers.
Skeletal Dysplasia (Non - Sclerosing dysplasias – Part II)
K Subbarao
Nepalese Journal of Radiology , 2014, DOI: 10.3126/njr.v4i1.11364
Abstract: DOI: http://dx.doi.org/10.3126/njr.v4i1.11364 Nepalese Journal of Radiology, Vol.4(1) 2014: 1-11
Product partitions and recursion formulae
M. V. Subbarao
International Journal of Mathematics and Mathematical Sciences , 2004, DOI: 10.1155/s0161171204307258
Abstract: Utilizing a method briefly hinted in the author's paper written in 1991 jointly with V. C. Harris, we derive here a number of unpublished recursion formulae for a variety of product partition functions which we believe have not been considered before in the literature. These include the functions p*(n;k,h) (which stands for the number of product partitions of n>1 into k parts of which h are distinct), and p(d)*(n;m) (which stands for the number of product partitions of n into exactly m parts with at most d repetitions of any part). We also derive recursion formulae for certain product partition functions without the use of generating functions.
H5N1 Viruses and Vaccines
Kanta Subbarao ,Catherine Luke
PLOS Pathogens , 2007, DOI: 10.1371/journal.ppat.0030040
Area Efficient and A High Bit Rate Serial-Serial Multiplier With On-the-Fly Accumulation by Asynchronous Counters
Balakrishna Konda,Subbarao Madasu
International Journal of Electronics Communication and Computer Technology , 2012,
Abstract: Traditional Serial-Serial multiplier addresses the high data sampling rate. It is effectively considered as the entire partial product matrix with n data sampling cycle for n×n multiplication function instead of 2n cycles in the conventional multipliers. This multiplication of partial products by considering two series inputs among which one is starting from LSB the other from MSB. Using this feed sequence and accumulation technique it takes only n cycle to complete the partial products. It achieves high bit sampling rate by replacing conventional full adder and highest 5:3 counters. Here asynchronous 1’s counter is presented. This counter takes critical path is limited to only an AND gate and D flip-flops. Accumulation is integral part of serial multiplier design. 1’s counter is used to count the number of ones at the end of the nth iteration in each counter produces. The implemented multipliers consist of a serial-serial data accumulator module and carry save adder that occupies less silicon area than the full carry save adder. In this paper we implemented model address for the 8bit 2’s complement implementing the Baugh-wooley algorithm and unsigned multiplication implementing the architecture for 8×8 Serial-Serial unsigned multiplication.
Antibacterial Potential of the Extracts of the Leaves of Azadirachta indica Linn.
Saradhajyothi KOONA,Subbarao BUDIDA
Notulae Scientia Biologicae , 2011,
Abstract: Azadirachta indica A. Juss (syn. Melia azadirachta) is well known in India and popularly known as Indian neem. To evaluate antibacterial potential, the agar well diffusion assay was used against Gram-negative and Gram-positive bacteria. Penicillin and Dimethyl sulfoxide were used as positive and negative controls, respectively. Methanol extract showed the highest and chloroform extract showed moderate to good antibacterial activity. Proteus vulgaris and Micrococcus luteus were the most susceptible bacteria while Bacillus subtilis was more resistant bacterium to the hexane, chloroform and methanol extracts of neem. The study recommended for the isolation and separation of bioactive compounds responsible for the antibacterial activity.
Click Efficiency: A Unified Optimal Ranking for Online Ads and Documents
Raju Balakrishnan,Subbarao Kambhampati
Computer Science , 2011,
Abstract: Traditionally the probabilistic ranking principle is used to rank the search results while the ranking based on expected profits is used for paid placement of ads. These rankings try to maximize the expected utilities based on the user click models. Recent empirical analysis on search engine logs suggests a unified click models for both ranked ads and search results. The segregated view of document and ad rankings does not consider this commonality. Further, the used models consider parameters of (i) probability of the user abandoning browsing results (ii) perceived relevance of result snippets. But how to consider them for improved ranking is unknown currently. In this paper, we propose a generalized ranking function---namely "Click Efficiency (CE)"---for documents and ads based on empirically proven user click models. The ranking considers parameters (i) and (ii) above, optimal and has the same time complexity as sorting. To exploit its generality, we examine the reduced forms of CE ranking under different assumptions enumerating a hierarchy of ranking functions. Some of the rankings in the hierarchy are currently used ad and document ranking functions; while others suggest new rankings. While optimality of ranking is sufficient for document ranking, applying CE ranking to ad auctions requires an appropriate pricing mechanism. We incorporate a second price based pricing mechanism with the proposed ranking. Our analysis proves several desirable properties including revenue dominance over VCG for the same bid vector and existence of a Nash Equilibrium in pure strategies. The equilibrium is socially optimal, and revenue equivalent to the truthful VCG equilibrium. Further, we relax the independence assumption in CE ranking and analyze the diversity ranking problem. We show that optimal diversity ranking is NP-Hard in general, and that a constant time approximation is unlikely.
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