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Search Results: 1 - 10 of 144276 matches for " Strouse B "
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Electrospun fiber membranes enable proliferation of genetically modified cells
Borjigin M,Eskridge C,Niamat R,Strouse B
International Journal of Nanomedicine , 2013,
Abstract: Mandula Borjigin*, Chris Eskridge*, Rohina Niamat, Bryan Strouse, Pawel Bialk, Eric B KmiecDepartment of Chemistry, Delaware State University, Dover, DE, USA *These authors contributed equally to this work Abstract: Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin) are well-established biomaterials that can enrich cell growth and enable tissue engineering. However, their application in the recovery and proliferation of genetically modified cells has not been studied. In the study reported here, we fabricated PCL-biomaterial blended fiber membranes, characterized them using physicochemical techniques, and used them as templates for the growth of genetically modified HCT116-19 colon cancer cells. Our data show that the blended polymers are highly miscible and form homogenous electrospun fiber membranes of uniform texture. The aligned PCL nanofibers support robust cell growth, yielding a 2.5-fold higher proliferation rate than cells plated on standard plastic plate surfaces. PCL-lecithin fiber membranes yielded a 2.7-fold higher rate of proliferation, while PCL-chitosan supported a more modest growth rate (1.5-fold higher). Surprisingly, PCL-gelatin did not enhance cell proliferation when compared to the rate of cell growth on plastic surfaces. Keywords: nanofibers, PCL-biomaterial blends, miscibility, gene editing, cell proliferation
Electrospun fiber membranes enable proliferation of genetically modified cells
Borjigin M, Eskridge C, Niamat R, Strouse B, Bialk P, Kmiec EB
International Journal of Nanomedicine , 2013, DOI: http://dx.doi.org/10.2147/IJN.S40117
Abstract: trospun fiber membranes enable proliferation of genetically modified cells Original Research (922) Total Article Views Authors: Borjigin M, Eskridge C, Niamat R, Strouse B, Bialk P, Kmiec EB Published Date February 2013 Volume 2013:8 Pages 855 - 864 DOI: http://dx.doi.org/10.2147/IJN.S40117 Received: 09 November 2012 Accepted: 16 December 2012 Published: 27 February 2013 Mandula Borjigin*, Chris Eskridge*, Rohina Niamat, Bryan Strouse, Pawel Bialk, Eric B Kmiec Department of Chemistry, Delaware State University, Dover, DE, USA *These authors contributed equally to this work Abstract: Polycaprolactone (PCL) and its blended composites (chitosan, gelatin, and lecithin) are well-established biomaterials that can enrich cell growth and enable tissue engineering. However, their application in the recovery and proliferation of genetically modified cells has not been studied. In the study reported here, we fabricated PCL-biomaterial blended fiber membranes, characterized them using physicochemical techniques, and used them as templates for the growth of genetically modified HCT116-19 colon cancer cells. Our data show that the blended polymers are highly miscible and form homogenous electrospun fiber membranes of uniform texture. The aligned PCL nanofibers support robust cell growth, yielding a 2.5-fold higher proliferation rate than cells plated on standard plastic plate surfaces. PCL-lecithin fiber membranes yielded a 2.7-fold higher rate of proliferation, while PCL-chitosan supported a more modest growth rate (1.5-fold higher). Surprisingly, PCL-gelatin did not enhance cell proliferation when compared to the rate of cell growth on plastic surfaces.
The Position of DNA Cleavage by TALENs and Cell Synchronization Influences the Frequency of Gene Editing Directed by Single-Stranded Oligonucleotides
Natalia Rivera-Torres, Bryan Strouse, Pawel Bialk, Rohina A. Niamat, Eric B. Kmiec
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096483
Abstract: With recent technological advances that enable DNA cleavage at specific sites in the human genome, it may now be possible to reverse inborn errors, thereby correcting a mutation, at levels that could have an impact in a clinical setting. We have been developing gene editing, using single-stranded DNA oligonucleotides (ssODNs), as a tool to direct site specific single base changes. Successful application of this technique has been demonstrated in many systems ranging from bacteria to human (ES and somatic) cells. While the frequency of gene editing can vary widely, it is often at a level that does not enable clinical application. As such, a number of stimulatory factors such as double-stranded breaks are known to elevate the frequency significantly. The majority of these results have been discovered using a validated HCT116 mammalian cell model system where credible genetic and biochemical readouts are available. Here, we couple TAL-Effector Nucleases (TALENs) that execute specific ds DNA breaks with ssODNs, designed specifically to repair a missense mutation, in an integrated single copy eGFP gene. We find that proximal cleavage, relative to the mutant base, is key for enabling high frequencies of editing. A directionality of correction is also observed with TALEN activity upstream from the target base being more effective in promoting gene editing than activity downstream. We also find that cells progressing through S phase are more amenable to combinatorial gene editing activity. Thus, we identify novel aspects of gene editing that will help in the design of more effective protocols for genome modification and gene therapy in natural genes.
Levinson's theorem for graphs
Andrew M. Childs,DJ Strouse
Physics , 2011, DOI: 10.1063/1.3622608
Abstract: We prove an analog of Levinson's theorem for scattering on a weighted (m+1)-vertex graph with a semi-infinite path attached to one of its vertices. In particular, we show that the number of bound states in such a scattering problem is equal to m minus half the winding number of the phase of the reflection coefficient (where each so-called half-bound state is counted as half a bound state).
Charge Ordering and Phase Competition in the Layered Perovskite Lasr2mn2o7
D. N Argyriou,H. N. Bordallo1,B. J. Campbell,A. K. Cheetham,D. E. Cox,J. S. Gardner,K. Hanif,A. dos Santos,G. F. Strouse
Physics , 1999, DOI: 10.1103/PhysRevB.61.15269
Abstract: Charge-lattice fluctuations are observed in the layered perovskite manganite LaSr2Mn2O7 by Raman spectroscopy as high as 340 K and with decreasing temperature they become static and form a charge ordered (CO) phase below TCO=210 K. In the static regime, superlattice reflections are observed through neutron and x-ray diffraction with a propagation vector (h+1/4,k-1/4,l). Crystallographic analysis of the CO state demonstrates that the degree of charge and orbital ordering in this manganite is weaker than the charge ordering in three dimensional perovskite manganites. A TN=170K a type-A antiferromagnetism (AF) develops and competes with the charge ordering, that eventually melts below T*=100K. High resolution diffraction measurements suggest that that CO- and AF-states do not coincide within the same region in the material but rather co-exist as separate phases. The transition to type-A antiferromagnetism at lower temperatures is characterized by the competition between these two phases.
Unitary equivalence to truncated Toeplitz operators
Elizabeth Strouse,Dan Timotin,Mohamed Zarrabi
Mathematics , 2010,
Abstract: In this paper we investigate operators unitarily equivalent to truncated Toeplitz operators. We show that this class contains certain sums of tensor products of truncated Toeplitz operators. In particular, it contains arbitrary inflations of truncated Toeplitz operators; this answers a question posed by Cima, Garcia, Ross, and Wogen.
Higher order Journe commutators and characterizations of multi-parameter BMO
Yumeng Ou,Stefanie Petermichl,Elizabeth Strouse
Mathematics , 2015,
Abstract: We consider iterated commutators of multiplication by a symbol function and tensor products of Hilbert or Riesz transforms. We establish mixed BMO classes of symbols that characterize boundedness of these objects in $L^p$. Little BMO and product BMO, big Hankel operators and iterated commutators are the base cases of our results. We use operator theoretical methods and existing profound results on iterated commutators for the Hilbert transform case, while the general result in several variables is obtained through the construction of a Journ\'e operator that models the behavior of the multiple Hilbert transform. Upper estimates for commutators with paraproduct free Journ\'e operators as well as weak factorisation results are proven.
Closed ideals of the algebra of absolutely convergent Taylor series
Jean Esterle,Elizabeth Strouse,Fouad Zouakia
Mathematics , 1994,
Abstract: Let $\Gamma$ be the unit circle, $A(\Gamma)$ the Wiener algebra of continuous functions whose series of Fourier coefficients are absolutely convergent, and $A^+$ the subalgebra of $A(\Gamma)$ of functions whose negative coefficients are zero. If $I$ is a closed ideal of $A^+$, we denote by $S_I$ the greatest common divisor of the inner factors of the nonzero elements of $I$ and by $I^A$ the closed ideal generated by $I$ in $A(\Gamma)$. It was conjectured that the equality $I^A= S_I H^\infty \cap I^A$ holds for every closed ideal $I$. We exhibit a large class $\scr F$ of perfect subsets of $\Gamma$, including the triadic Cantor set, such that the above equality holds whenever $h(I)\cap\Gamma\in\scr F$. We also give counterexamples to the conjecture.
Systematic evaluation of an atomic clock at 2e-18 total uncertainty
T. L. Nicholson,S. L. Campbell,R. B. Hutson,G. E. Marti,B. J. Bloom,R. L. McNally,W. Zhang,M. D. Barrett,M. S. Safronova,G. F. Strouse,W. L. Tew,J. Ye
Physics , 2014, DOI: 10.1038/ncomms7896
Abstract: The pursuit of better atomic clocks has advanced many research areas, providing better quantum state control, new insights in quantum science, tighter limits on fundamental constant variation, and improved tests of relativity. The record for the best stability and accuracy is currently held by optical lattice clocks. This work takes an important step towards realizing the full potential of a many-particle clock with a state-of-the-art stable laser. Our 87Sr optical lattice clock now achieves fractional stability of 2.2e-16 at 1 s. With this improved stability, we perform a new accuracy evaluation of our clock, reducing many systematic uncertainties that limited our previous measurements, such as those in the lattice ac Stark shift, the atoms' thermal environment, and the atomic response to room-temperature BBR. Our combined measurements have reduced the total uncertainty of the JILA Sr clock to 2.1e-18 in fractional frequency units.
Ultra-Sensitive Chip-Based Photonic Temperature Sensor Using Ring Resonator Structures
Haitan Xu,Mohammad Hafezi,J. Fan,J. M. Taylor,G. F. Strouse,Zeeshan Ahmed
Physics , 2013, DOI: 10.1364/OE.22.003098
Abstract: Resistance thermometry provides a time-tested method for taking temperature measurements. However, fundamental limits to resistance-based approaches has produced considerable interest in developing photonic temperature sensors to leverage advances in frequency metrology and to achieve greater mechanical and environmental stability. Here we show that silicon-based optical ring resonator devices can resolve temperature differences of 1 mK using the traditional wavelength scanning methodology. An even lower noise floor of 80 microkelvin for measuring temperature difference is achieved in the side-of-fringe, constant power mode measurement.
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