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Search Results: 1 - 10 of 6062 matches for " Stefano Guizzardi "
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Hyaluronate Increases Polynucleotides Effect on Human Cultured Fibroblasts  [PDF]
Stefano Guizzardi,Jacopo Uggeri,Silvana Belletti,Giulia Cattarini
Journal of Cosmetics, Dermatological Sciences and Applications (JCDSA) , 2013, DOI: 10.4236/jcdsa.2013.31019
Abstract: The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also polynucleotydes (PN) have proved to promote the “in vitro” growth and activity of human fibroblasts and osteoblasts, to increase reparation on UVB damaged dermal fibroblasts and seems to promote proliferation of human pre-adipocytes. Several in vivo studies have demonstrated the PN effect also in vivo, inducing an increase of angiogenesis and healing process. In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth. Different mixture have been tested and it has been demonstrated that the presence of HA even at low concentration (1 mg/ml) determine an increase of PN activity up to 20%. Furthermore, the addition of HA 1 mg/ml to PN 100 μg/ml induces a cell growth rate comparable to that exerted by PN concentration of 12 μg/ml.
Hyaluronate Increases Polynucleotides Effect on Human Cultured Fibroblasts  [PDF]
Stefano Guizzardi, Jacopo Uggeri, Silvana Belletti, Giulia Cattarini
Journal of Cosmetics, Dermatological Sciences and Applications (JCDSA) , 2013, DOI: 10.4236/jcdsa.2013.31019
Abstract:

The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also polynucleotydes (PN) have proved to promote the “in vitro” growth and activity of human fibroblasts and osteoblasts, to increase reparation on UVB damaged dermal fibroblasts and seems to promote proliferation of human pre-adipocytes. Several in vivo studies have demonstrated the PN effect also in vivo, inducing an increase of angiogenesis and healing process. In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth. Different mixture have been tested and it has been demonstrated that the presence of HA even at low concentration (1 mg/ml) determine an increase of PN activity up to 20%. Furthermore, the addition of HA 1 mg/ml to PN 100 μg/ml induces a cell growth rate comparable to that exerted by PN concentration of 12 μg/ml.

Anti Inflammatory Property of PDRN—An in Vitro Study on Cultured Macrophages  [PDF]
Chiara Castellini, Silvana Belletti, Paolo Govoni, Stefano Guizzardi
Advances in Bioscience and Biotechnology (ABB) , 2017, DOI: 10.4236/abb.2017.81002
Abstract: Skin aging and most age-related diseases are associated with a low-grade chronic inflammation. The nucleoside adenosine, a potent endogenous anti-inflammatory agent, is deeply involved in inflammatory diseases and, by interaction with the adenosine A2 receptor (A2AR) it immediately promotes a mechanism of defence against the inflammatory damage. The aim of our study was to investigate whether polydeoxyribonucleotide (PDRN), a mixture of deoxyribonucleotides polymers of different lengths that like adenosine, binds the A2A receptor, can reduce the inflammatory state in the macrophage cell line. RAW264.7, murine macrophage cells, were incubated with PDRN in the presence and in the absence of lipopolysaccaride (LPS), which was the major component of the outer membrane of gram-negative bacteria and which acted as a strong macrophage activator. We assessed the production of nitric oxide and the secretion of inflammatory mediators (i.e., TNF-α, IL-10, IL-12 and VEGF-A). Our data showed that PDRN produced a significant decrease of inflammation in macrophages pre-stimulated with LPS, assessed in terms of the nitric oxide content (p < 0.001) and cytokines secretion (p < 0.001). Moreover, PDRN stimulated the release of the vascular endothelial growth factor (VEGF-A), which promoted wound healing. Our study suggested that PDRN, by binding the A2A receptor, contributed to a great extent towards reducing inflammation.
Analyzing requirements of knowledge management systems with the support of agent organizations
Guizzardi, Renata S. S.;Perini, Anna;
Journal of the Brazilian Computer Society , 2005, DOI: 10.1007/BF03192370
Abstract: knowledge management (km) is considered by many organizations a key aspect in sustaining competitive advantage. designing appropriate km processes and enabling technology face considerable risks, as they must be shaped to respond to specific needs of the organizational environment. thus, many systems are abandoned or fall into disuse because of inadequate understanding of the organizational context. this motivates current research, which tends to propose agent organizations as a useful paradigm for km systems engineering. following these approaches, organizations are analyzed as collective systems, composed of several agents, each of them autonomously producing and managing their own local data according to their own logic, needs, and interpretative schema, i.e. their goals and beliefs. these agents interact and coordinate for goal achievement defining a coherent local knowledge system. this paper presents a novel methodology for analyzing the requirements of a km system based on an iterative workflow where a pivotal role is played by agent-oriented modeling. within this approach, the needs for km systems are traced back to the organization stakeholders' goals. a case study is used to illustrate the methodology. the relationship of this work with current studies in agent organizations and organizational knowledge management is also discussed. differently from other works, this methodology aims at offering a practical guideline to the analyst, pointing out the appropriate abstractions to be used in the different phases of the analysis.
An ontological analysis of the electrocardiogram - DOI: 10.3395/reciis.v3i1.242en
Bernardo Gon?alves,Veruska Zamborlini,Giancarlo Guizzardi
RECIIS : Electronic Journal of Communication, Information & Innovation in Health , 2009,
Abstract: Bioinformatics has been a fertile field for the application of the discipline of formal ontology. The principled representation of biomedical entities has increasingly supported biological research, with direct benefits ranging from the reformulation of medical terminologies to the introduction of new perspectives for enhanced models of Electronic Health Records (EHR). This paper introduces an application-independent ontological analysis of the electrocardiogram (ECG) grounded in the Unified Foundational Ontology. With the objective of investigating the phenomena underlying this cardiological exam, we deal with the sub-domains of human heart electrophysiology and anatomy. We then outline an ECG Ontology built upon the OBO Relation Ontology. In addition, the domain ontology sketched here takes inspiration both in the Foundational Model of Anatomy and in the Ontology of Functions proposed under the auspices of the General Formal Ontology (GFO) research program.
Feasibility Evaluation of Integrating UsabilityEngineering Issues in a Design for Multi-XCollaborative Framework  [PDF]
Stefano Filippi
Intelligent Information Management (IIM) , 2011, DOI: 10.4236/iim.2011.31004
Abstract: Design for manufacturing, design for assembly, and, in general, design for X, are methods helping an effective generation of industrial products. In parallel with the development of these methods, the research about usability engineering has generated many important results, both from the design, and the evaluation and testing points of view. The research described in this paper aims at evaluating the feasibility of the integration of two new usability methods, the design for innovative usability - DFIU -, and the integrated method for usability evaluation and testing - IMUET -, in an existing design for X named design guidelines collaborative framework - DGLs-CF -. Indeed, the DGLs-CF is a design for multi-X method, given that it covers both the manufacturing and the verification phases of the industrial product lifecycle. All these methods are currently under development by the author’s research group. To evaluate this feasibility, the first task of the research aims at describing and classifying the components of the three methods. Next, these components are semantically related to each other. Finally, the last activity verifies the compatibility between the components of the two usability methods and the data structures of the DGLs-CF to check the feasibility from the implementation point of view. The result of this research will consist of precise indications both for the development of a design for multi-X collaborative framework covering homogeneously the design, manufacturing, verification, and use phases of the industrial product lifecycle, and to be used as a reference for researchers interested in considering the integration of usability issues in their design tools, methods, and processes.
A General Purpose Analysis Package  [PDF]
Stefano Federico
Atmospheric and Climate Sciences (ACS) , 2012, DOI: 10.4236/acs.2012.22022
Abstract: This paper presents a general-purpose analysis package able to solve two- and three- dimensional analysis problems. The system can use the following methods of solution: Successive Approximation (SA), Optimal Interpolation (OI), and 3D-Var. Analyses are given for the following parameters: zonal and meridional wind components, temperature, relative humidity, and geopotential height. The analysis package was applied to produce analyses at 6 h time interval for the period 1-11 August 2008. The period was selected for data availability and forty-one analyses were collected. The results show the validity of the different solutions, which can be chosen depending on the physical problem to solve and on the computational resources available. In particular, assuming the observations as the reference, all solutions show a decrease of the RMSE compared to the background. The decrease is consistent with the particular setting of the analysis system used in this paper. The comparison between different solutions shows that the SA converges to OI in few iterations, and that the SA solution with ten iteration is, in practice, equal to OI. Moreover, the 3D-Var method shows its potential to improve the analysis, once the horizontal and vertical length-scales and the background and observational errors are set optimally, because its solution may be sizeably different from two-dimensional methods.
Preliminary Results of a Data Assimilation System  [PDF]
Stefano Federico
Atmospheric and Climate Sciences (ACS) , 2013, DOI: 10.4236/acs.2013.31009
Abstract:

A data assimilation system combines all available information on the atmospheric state in a given time-window to produce an estimate of atmospheric conditions valid at a prescribed analysis time. Nowadays, increased computing power coupled with greater access to real-time asynoptic data is paving the way toward a new generation of high-resolution (i.e. on the order of 10 km) operational mesoscale analyses and forecasting systems. Moreover, better initial conditions are increasingly considered of the utmost importance for Numerical Weather Prediction (NWP) at the short range (0 - 12 h). This paper presents a general-purpose data assimilation system, which is coupled with the Regional Atmospheric Modelling System (RAMS) to give the analyses for: zonal and meridional wind components, temperature, relative humidity, and geopotential height. In order to show its potential, the data assimilation systems applied to produce analyses over Central Europe. For this application the background field is given by a short-range forecast (12 h) of the RAMS and analyses are produced by 2D-Var with 0.25? horizontal resolution. Results show the validity of the analyses because they are closer to the observations, consistently with the settings of the data assimilation system. To quantify the impact of improved initial conditions on the forecast, the analyses are then used as initial conditions of a short-range (6 h) forecast of the RAMS model. The results show that the RMSE is effectively reduced for the one- and two hours forecast, with some improvement for the three-hours forecast.

Continued Fractions and Dynamics  [PDF]
Stefano Isola
Applied Mathematics (AM) , 2014, DOI: 10.4236/am.2014.57101
Abstract:

Several links between continued fractions and classical and less classical constructions in dynamical systems theory are presented and discussed.


Revealed Cores: Characterizations and Structure  [PDF]
Stefano Vannucci
Applied Mathematics (AM) , 2015, DOI: 10.4236/am.2015.614200
Abstract: Characterizations of the classes of all choice functions that select the cores or the externally stable cores induced by an underlying revealed dominance digraph are provided. Relying on such characterizations, the basic order-theoretic structure of the corresponding sets of revealed cores is also analyzed. In particular, it is shown that the poset of all revealed cores ordered by set inclusion is a median meet semilattice: therefore, any profile of revealed cores may be aggregated by means of the simple majority rule.
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