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Search Results: 1 - 10 of 680 matches for " Steen Holmberg "
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Mediator regulates non-coding RNA transcription at fission yeast centromeres
Michael Thorsen, Heidi Hansen, Michela Venturi, Steen Holmberg, Genevieve Thon
Epigenetics & Chromatin , 2012, DOI: 10.1186/1756-8935-5-19
Abstract: We found that the Med8-Med18-Med20 submodule of the Mediator complex is required for the transcriptional regulation of native centromeric dh and dg repeats and for the silencing of reporter genes inserted in centromeric heterochromatin. Mutations in the Med8-Med18-Med20 submodule did not alter Mediator occupancy at centromeres; however, they led to an increased recruitment of RNA polymerase II to centromeres and reduced levels of centromeric H3K9 methylation accounting for the centromeric desilencing. Further, we observed that Med18 and Med20 were required for efficient processing of dh transcripts into siRNA. Consistent with defects in centromeric heterochromatin, cells lacking Med18 or Med20 displayed elevated rates of mitotic chromosome loss.Our data demonstrate a role for the Med8-Med18-Med20 Mediator submodule in the regulation of non-coding RNA transcription at Schizosaccharomyces pombe centromeres. In wild-type cells this submodule limits RNA polymerase II access to the heterochromatic DNA of the centromeres. Additionally, the submodule may act as an assembly platform for the RNAi machinery or regulate the activity of the RNAi pathway. Consequently, Med8-Med18-Med20 is required for silencing of centromeres and proper mitotic chromosome segregation.Mediator is a large (approximately 1 MDa) protein complex that conveys regulatory signals to RNA polymerase II (Pol II). The Saccharomyces cerevisiae Mediator was the first to be characterized but Mediators have since then been described in many other species. A comparative genomics approach of approximately 70 eukaryotic genomes shows that although its exact subunit composition varies, Mediator is conserved across the eukaryotic kingdom [1]. The Schizosaccharomyces pombe Mediator consists of at least 20 subunits, all of which appear to have orthologues in Drosophila melanogaster, Caenorhabditis elegans and Homo sapiens[2].Three distinct domains (head, middle and tail) have been identified by electron microscopy on
MRN1 Implicates Chromatin Remodeling Complexes and Architectural Factors in mRNA Maturation
Louis Düring, Michael Thorsen, Darima Sophia Njama Petersen, Brian K?ster, Torben Heick Jensen, Steen Holmberg
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044373
Abstract: A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6ΔΔ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6ΔΔ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6ΔΔ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6ΔΔ) as a growth suppressor of rsc nhp6ΔΔ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 μm-MRN1 and the splicing deficient mutants snt309Δ, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.
Wine fraud
Lars Holmberg
International Journal of Wine Research , 2010, DOI: http://dx.doi.org/10.2147/IJWR.S14102
Abstract: e fraud Review (5431) Total Article Views Authors: Lars Holmberg Published Date October 2010 Volume 2010:2 Pages 105 - 113 DOI: http://dx.doi.org/10.2147/IJWR.S14102 Lars Holmberg Faculty of Law, University of Copenhagen, Copenhagen, Denmark Abstract: Wine fraud may take several forms, of which two are discussed here: consumption fraud aimed at the wine market in general, and collector fraud aimed at the very top of the wine market. Examples of wine fraud past and present are given, and a suggestion about the extent of contemporary consumer fraud in Europe is provided. Technological possibilities for future detection and prevention of both forms of wine fraud are discussed.
Sp r av l rande – f rem l som k lla
Annelie Holmberg
Techne Series : Research in Sloyd Education and Craft Science A , 2011,
Abstract: Kan f rem l som k lla bidra i forskning om undervisning? L mnar olika tiders l randetraditioner sp r i de f rem l som tillverkas under utbildning och vilka metoder kan anv ndas d f rem len studeras? Syftet med artikeln r att i n gon m n besvara dessa fr gor, att f rmedla ett exempel p forskning d r f rem len varit avg rande f r resultatet. Verbalisera den metod som anv nts, analysera den samt relatera till tidigare dokumenterade metoder som anv nts inom till exempel traditionen Material culture. Visa att f rem l framst llda av textill rarstuderande under utbildning kan ber tta n got om utbildningskulturen de var en del av. De tv f rem l som h r studeras r tv broderier utf rda av studerande inom textill rarutbildningen i Uppsala. En v ska med en broderad, stiliserad ros och ett dekorativt broderi med surialistisk m nsterbild att h nga p v ggen. Genom stygn och applikationer materialiseras ett l rande i de uppgifter som l rarutbildarna utformar. Stringent och uts kt eller kreativt och personligt r ord som beskriver de b da analysobjekten, orden beskriver olika tiders m ls ttningar med l rande. L rande r en process som kan p g i livets alla situationer och r d rmed inte begr nsad till undervisning, l rande r en aktiv f r ndring av en m nniska (S lj , 2000). Jag tolkar h r l rande som en process d r studenten r aktiv. Studentens l rande l mnar sp r, meddelanden, som kan ber tta om s v l studentans som utbildningens l randeprocesser och intentioner. Det l rande som i denna artikel studeras r kopplat till en sluten utbildning och utbildningskultur. F rem len terfinns ven i en mer omfattande analys d r ven intervjuer och dokument analyseras (Holmberg, 2009). S kord: metod, sl jd, f rem l, l rarutbildning, utbildningstradition URN:NBN:no-29970
Lessons learned from the birth and evolution of the EduFinland virtual community for educators
Kim Holmberg
Knowledge Management & E-Learning : an International Journal , 2012,
Abstract: Educators around the world are experimenting with the possibilities virtual three dimensional worlds have for education and learning how to use these new environments efficiently. Virtual worlds have the potential to bring some new added value to education and educators can use them to create something that is not possible to do or show in traditional classrooms. Although a lot have been learned about virtual worlds and their potential, a lot more has to be learned before virtual worlds can become an integrated part of education on various levels. The article looks back at the birth and growth of the EduFinland community in the virtual world of Second Life and discusses lessons learned so far during the years of its existence.
Review: Judith Green & Nicki Thorogood (2004). Qualitative Methods for Health Research Review: Judith Green & Nicki Thorogood (2004). Qualitative Methods for Health Research Rese a: Judith Green & Nicki Thorogood (2004). Qualitative Methods for Health Research
Christine Holmberg
Forum : Qualitative Social Research , 2006,
Abstract: GREEN und THOROGOOD legen mit Qualitative Methods for Health Research ein fundiertes und pr zises Einführungsbuch vor. Der Aufbau des Bandes ist dem organisatorischen Ablauf eines Forschungsprojekts angepasst. Es beginnt mit zugrunde liegenden Epistemologien qualitativer Methoden, diskutiert darauf aufbauend Fragestellungen, die bearbeitet werden k nnen, und beschreibt eine Vielzahl qualitativer Methoden und Auswertungsm glichkeiten. Den Abschluss des Buches bildet eine Diskussion der Besonderheiten von qualitativen Forschungsstudien im Gesundheitsbereich, indem deren besondere Schwierigkeiten und M glichkeiten ausgeleuchtet werden. Für all diejenigen, die einzelne Themenbereiche vertiefen m chten, gibt es am Ende jeden Kapitels weiterführende Literaturhinweise. GREEN und THOROGOOD vermeiden zugespitzte epistemologische Debatten. Diese Neutralit t macht das Buch zu einer sehr guten, klaren Einführung in die Praxis qualitativer Forschung für Gesundheitswissenschaftlerinnen und Gesundheitswissenschaftler, die qualitative Methoden in ihre Arbeit aufnehmen wollen, oder für Novizen und Novizinnen, die erstmals einen qualitativ-empirischen Zugang zu Fragen der Gesundheitsforschung suchen. URN: urn:nbn:de:0114-fqs060274 "Qualitative Methods for Health Research" is a pragmatic introduction to qualitative research in the health sciences. It is written for professional researchers with little knowledge of the social sciences. It serves that audience well. The book provides a comprehensive overview of different epistemologies underlying qualitative methodologies and of a broad range of qualitative methods. It illustrates different approaches with numerous case studies and adds a list of further readings at the end of each chapter. It is an excellent source of information for all those unfamiliar with qualitative research. URN: urn:nbn:de:0114-fqs060274 "Métodos cualitativos para la investigación en salud" es una introducción a la investigación cualitativa en las ciencias de la salud. Está escrito para investigadores profesionales con poco conocimiento de las ciencias sociales. Sirve bien a esa audiencia. El libro formula una visión comprensiva de las epistemologías subyacentes a las metodologías cualitativas, y un amplio rango de métodos cualitativos. Ilustra diferentes aproximaciones con numerosos casos de estudio y aporta una lista de lecturas adicionales al final de cada capítulo. Es una excelente fuente de información para los no familiarizados con la investigación cualitativa. URN: urn:nbn:de:0114-fqs060274
Questions, Answers, Polarity and Head Movement in Germanic and Finnish
Anders Holmberg
Nordlyd : Troms? University Working Papers on Language & Linguistics / Institutt for Spr?k og Litteratur, Universitetet i Troms? , 2003,
Abstract: The paper investigates the consequences of combining the following two assumptions: (a) The English negation n’t is an inflection, and (b) suffixed forms are derived in the syntax by head movement with left-adjunction. An immediate consequence is that Neg must c-command T. This entails that inversion in negative yes/no-questions (YNQs) is not T-to-C but Neg-to-C, or, if Neg is Pol(arity) with negative value, Pol-to-C. This in turn makes possible viewing inversion in YNQs as a special case of wh-movement. It also makes possible analyzing inversion in Germanic as essentially the same as in Finnish, where the negation overtly undergoes movement in negative YNQs. It also provides the basis for a theory of the syntax of replies toYNQs, including negative questions.
Wine fraud
Lars Holmberg
International Journal of Wine Research , 2010,
Abstract: Lars HolmbergFaculty of Law, University of Copenhagen, Copenhagen, DenmarkAbstract: Wine fraud may take several forms, of which two are discussed here: consumption fraud aimed at the wine market in general, and collector fraud aimed at the very top of the wine market. Examples of wine fraud past and present are given, and a suggestion about the extent of contemporary consumer fraud in Europe is provided. Technological possibilities for future detection and prevention of both forms of wine fraud are discussed.Keywords: adulteration, counterfeit, detection
A Note on the Variability of V538 Cassiopeiae
Gustav Holmberg
Physics , 2012,
Abstract: CCD observations of V538 Cas have been made on nine nights during three weeks using the AAVSO Bright Star Monitor. No significant variations were found.
Inter-organizational cooperation: A rehabilitation project based on cooperation between health care and three social service agencies  [PDF]
Nils-Gunnar Rudenstam, Leif Holmberg
Health (Health) , 2014, DOI: 10.4236/health.2014.65050
Abstract: Purpose: Cooperation between organizations is an often-suggested remedy for handling unsolved borderland problems. However, actual projects aiming at cooperation are seldom very successful. The purpose here is to highlight obstacles related to cooperation between different organizations based on a case study of a rehabilitation project where health care and several social service organizations (social insurance, social welfare, and the local employment agency) were involved. Data were gathered through participation and interviews. Findings: It seems that efficient cooperation requires an understanding of the participating organizations’ differences in work logic as well as work practices. Furthermore, only certain fairly standardized “normal” problems may be handled through organized cooperation while non-routine exceptional problem requires a more fully integrated work organization. Implications: Obstacles to cooperation are highlighted and ways to improve the possibilities of cooperation between organizations are suggested although such possibilities are generally hampered by differences in work logic.
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