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Search Results: 1 - 10 of 253 matches for " Sofie Hagens "
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The Dark Side of EGFP: Defective Polyubiquitination
Mathijs Baens, Heidi Noels, Vicky Broeckx, Sofie Hagens, Sabine Fevery, An D. Billiau, Hugo Vankelecom, Peter Marynen
PLOS ONE , 2006, DOI: 10.1371/journal.pone.0000054
Abstract: Enhanced Green Fluorescent Protein (EGFP) is the most commonly used live cell reporter despite a number of conflicting reports that it can affect cell physiology. Thus far, the precise mechanism of GFP-associated defects remained unclear. Here we demonstrate that EGFP and EGFP fusion proteins inhibit polyubiquitination, a posttranslational modification that controls a wide variety of cellular processes, like activation of kinase signalling or protein degradation by the proteasome. As a consequence, the NF-κB and JNK signalling pathways are less responsive to activation, and the stability of the p53 tumour suppressor is enhanced in cell lines and in vivo. In view of the emerging role of polyubiquitination in the regulation of numerous cellular processes, the use of EGFP as a live cell reporter should be carefully considered.
Interviewee Transcript Review: assessing the impact on qualitative research
Victoria Hagens, Mark J Dobrow, Roger Chafe
BMC Medical Research Methodology , 2009, DOI: 10.1186/1471-2288-9-47
Abstract: ITR was incorporated into a qualitative research study in which 51 key informant interviews were conducted with a range of senior stakeholders within the Canadian health care system. The changes made by interviewees to their transcripts were systematically tracked and categorized using a set of mutually exclusive categories.The study found that ITR added little to the accuracy of the transcript and may create complications if the goal of the researcher is to produce a transcript which reflects precisely what was said at the time of the interview. The advantages of ITR are that it allows interviewees the opportunity to edit or clarify information provided in the original interview, with many interviewees providing corrections, clarifications, and in some cases, adding new material to their transcripts. There are also potential disadvantages, such as a bias created by inconsistent data sources or the loss of data when an interviewee chooses to remove valuable material. The impact of ITR on the interviewee may be both positive and negative, depending on the specific circumstances and the nature of the study. The impact of ITR on the researcher was minimal in this study, but is again subject to specific circumstances of the research context.While ITR is employed by many researchers across numerous fields, the advantages of its use may be relatively small in terms of verifying the accuracy of qualitative interview transcripts. Researchers are advised to carefully consider both the potential advantages and disadvantages of ITR outlined in this paper before deciding to incorporate the practice within their qualitative study designs.As part of the qualitative research process, researchers in a variety of fields, such as health [1-3], education[4,5], management[6,7] and social theory[8,9], often invite interviewees to review transcripts of their interviews. We refer to this practice, in its various forms, as interviewee transcript review (ITR). ITR can include requests for i
Altru stisch ambtenaar of hero sch genie? Het gepropageerde beeld van provinciale en academische directeurs van bacteriologische laboratoria in Belgi (ca. 1900-1940)
Sofie Onghena
Studium : Tijdschrift voor Wetenschaps- en Universiteits-Geschiedenis , 2009,
Abstract: Altruistic public servant or heroic genius? The propagated image of provincial and academic directors of bacteriological laboratories in Belgium (ca. 1900-1940) At the end of the nineteenth century provincial bacteriological institutes were established in Belgium – in Liège, Mons, Namur and Brussels – in order to combat epidemics, to promote preventive medicine and to pursue the successful research of Louis Pasteur and Robert Koch. Similar laboratories existed at the universities of Ghent, Louvain and Brussels. The image building played an important role for both kinds of institutes, as bacteriology in pioneering phase had to be publicly confirmed as a new, valuable discipline. However, the directors of provincial and academic institutes – with the same academic training though – were awarded with different qualities at their jubilees, fitting with the purposes and the self-image of their respective institutions, either provincial authorities or universities. The image of academic directors was guided by academic decorum: Emile van Ermengem, Edmond Destrée and Joseph Denys were represented as savants, solely devoted to pure science and paternally educating young researchers, notwithstanding the fact that their laboratories had humanitarian merits as well. On the other hand, the discourse on the first provincial directors – Ernest Malvoz, Martin Herman, Achille Haibe – emphasized their altruistic commitment and their solid work for the provincial government. Jules Bordet, internationally rewarded scientist, professor and provincial director of the Pasteur Institute in Brussels, was celebrated with both sorts of discourses.
REVIEW: Cosmopolitanism: Ideals and Realities
Sofie Dreef
Amsterdam Law Forum , 2012,
Abstract: Book review of 'Cosmopolitanism: Ideals and Realities' by David Held.
Sofie Dreef
Amsterdam Law Forum , 2011,
Abstract: The topic of Amsterdam Law Forum’s last thematic number is ‘Legal Perspectives on Gender and Sexual Equality’.
Sofie Dreef
Amsterdam Law Forum , 2011,
Enhanced Sensitivity to Rapid Input Fluctuations by Nonlinear Threshold Dynamics in Neocortical Pyramidal Neurons
Skander Mensi?,Olivier Hagens,Wulfram Gerstner?,Christian Pozzorini
PLOS Computational Biology , 2016, DOI: 10.1371/journal.pcbi.1004761
Abstract: The way in which single neurons transform input into output spike trains has fundamental consequences for network coding. Theories and modeling studies based on standard Integrate-and-Fire models implicitly assume that, in response to increasingly strong inputs, neurons modify their coding strategy by progressively reducing their selective sensitivity to rapid input fluctuations. Combining mathematical modeling with in vitro experiments, we demonstrate that, in L5 pyramidal neurons, the firing threshold dynamics adaptively adjust the effective timescale of somatic integration in order to preserve sensitivity to rapid signals over a broad range of input statistics. For that, a new Generalized Integrate-and-Fire model featuring nonlinear firing threshold dynamics and conductance-based adaptation is introduced that outperforms state-of-the-art neuron models in predicting the spiking activity of neurons responding to a variety of in vivo-like fluctuating currents. Our model allows for efficient parameter extraction and can be analytically mapped to a Generalized Linear Model in which both the input filter—describing somatic integration—and the spike-history filter—accounting for spike-frequency adaptation—dynamically adapt to the input statistics, as experimentally observed. Overall, our results provide new insights on the computational role of different biophysical processes known to underlie adaptive coding in single neurons and support previous theoretical findings indicating that the nonlinear dynamics of the firing threshold due to Na+-channel inactivation regulate the sensitivity to rapid input fluctuations.
Illustration af fremtidens arealanvendelse til energiafgr der p landskabsskala i et GIS
Ann-Sofie Richardt
Geoforum Perspektiv , 2010,
“Imagined Communities, Recuperated Homelands. Rethinking American and Canadian Minority and Exilic Writing”
Sofie De Smyter
Transatlantica : Revue d'études Américaines , 2012,
Abstract: The question “Where are you from?”, which is often heard at conferences, foregrounds the link between identity and location. This link proved constitutive of Imagined Communities, Recuperated Homelands, a conference that was organized by Professors Monica Manolescu and Charlotte Sturgess and took place at the University of Strasbourg on the eleventh and twelfth of March 2011. At the heart of the conference was the way Canadian and North American minority and exilic writing questions and recon...
Conference Report
Sofie Dreef,Laura Morrison
Amsterdam Law Forum , 2011,
Abstract: Amsterdam Law Forum Conference Legal Perspectives on Gender And Sexual Equality May 13, 2011 Aurorazaal – VU University Amsterdam, the Netherlands
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