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Search Results: 1 - 10 of 77980 matches for " Sitong Chen "
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The Research for APG-12 Emulsifying Properties  [PDF]
Jigang Wang, Sitong Chen, Yongle Xin, Yong’an Wang
Open Journal of Composite Materials (OJCM) , 2015, DOI: 10.4236/ojcm.2015.51004
Abstract: Through determining and evaluating interfacial tension and emulsifying properties of dodecyl polyglucoside (APG-12), the results show that APG-12’s performance is better than C12 linear alkylbenzene sulfonate (LAS) and alkyl phenol polyoxyethylene ether (OP-10). In order to emulsify properties of APG-12, it is more stable when concentration is over 1.5 g/L. We studied the temperature and oil-water ratio has an effect on emulsifying property.
Synthesis and Characterization of APG-12  [PDF]
Jigang Wang, Yongle Xin, Danting Fan, Sitong Chen
Open Journal of Composite Materials (OJCM) , 2015, DOI: 10.4236/ojcm.2015.51003
Abstract: APG-12 was synthesized from n-dodecyl alcohol and glucose in catalysis system of p-Toluenesulfonic and phosphoric acid. Using the method of direct glycosidation, this paper discusses the impact of raw material ratio, catalyst dosage and reaction temperature on the synthesis. It is concluded that the synthesis of APG-12 best reaction process condition is the molar ratio of alcohol and glucose 7:1; the reaction temperature is 115 and the amount of catalyst is 1.3%, controlling the reaction pressure in 3 - 4 kPa. The resulting products are analyzed by infrared spectroscopy.
Genome-scale identification of Caenorhabditis elegans regulatory elements by tiling-array mapping of DNase I hypersensitive sites
Baochen Shi, Xiangqian Guo, Tao Wu, Sitong Sheng, Jie Wang, Geir Skogerb?, Xiaopeng Zhu, Runsheng Chen
BMC Genomics , 2009, DOI: 10.1186/1471-2164-10-92
Abstract: Here, we report the first genome-wide analysis of DNase I hypersensitive sites (DHSs) in Caenorhabditis elegans. The data was obtained by hybridizing DNase I-treated and end-captured material from young adult worms to a high-resolution tiling microarray. The data show that C. elegans DHSs were significantly enriched within intergenic regions located 2 kb upstream and downstream of coding genes, and also that a considerable fraction of all DHSs mapped to intergenic positions distant to annotated coding genes. Annotated transcribed loci were generally depleted in DHSs relative to intergenic regions, but DHSs were nonetheless enriched in coding exons and UTRs, whereas introns were significantly depleted in DHSs. Many DHSs appeared to be associated with annotated non-coding RNAs and recently detected transcripts of unknown function. It has been reported that nematode highly conserved non-coding elements were associated with cis-regulatory elements, and we also found that DHSs, particularly distal intergenic DHSs, were significantly enriched in regions that were conserved between the C. elegans and C. briggsae genomes.We describe the first genome-wide analysis of C. elegans DHSs, and show that the distribution of DHSs is strongly associated with functional elements in the genome.With full genome sequences available for a number of species, it is now possible to extract further information on how the genome is functionally organized. Identification of regulatory elements of both coding and non-coding genes is therefore a major challenge of the post-genomics era. Caenorhabditis elegans is an important multicellular model organism for research on functional genomics and developmental biology, and has delivered a wealth of information with relevance also to research on human diseases and aging. C. elegans was the first to metazoan to have its genome sequenced [1], however, C. elegans genome annotation and molecular functional research have thus far mainly focused on the tran
A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus
Yongping Wen, Anchun Cheng, Mingshu Wang, Han Ge, Chanjuan Shen, Sitong Liu, Jun Xiang, Renyong Jia, Dekang Zhu, Xiaoyue Chen, Bei Lian, Hua Chang, Yi Zhou
Virology Journal , 2010, DOI: 10.1186/1743-422x-7-77
Abstract: In this study, we describe the expression and purification of a recombinant Thymidine Kinase (TK) protein which makes antigen in an in-house developed, optimized and standardized ELISA. The specificity of the optimized TK-ELISA was evaluated by antisera against Duck Plague Virus (DPV), Duck Hepatitis B Virus (DHBV), Duck Hepatitis Virus (DHV), Riemerella Anatipestifer(R. A), Escherichia coli (E. coli) and Salmonella anatum (S. anatum). Only antisera against DPV yielded a specific and strong signal. In order to determine the sensitivity of the TK-ELISA, a panel of diluted sera was tested, and the minimum detection limit of 1:2560 (OD450 nm = 0.401) was obtained according to the endpoint cut-off (0.2438). The repeatability and reproducibility under the experimental conditions demonstrates a low variability (P > 0.05). The suspected sera samples (n = 30) were determined by TK-ELISA and the positive rate is 90% (27/30), and the TK-ELISA showed 83.33% (22+3/30) coincidence rate with the Serum Neutralization Test (SNT) and 90% (24+3/30) coincidence rate with the whole DPV virion based-ELISA (DPV-ELISA). When defining the dynamics of antibody response to attenuated live DPV vaccine, the maximum antibodies is reached after 4 weeks.The results suggest that the TK-ELISA provides high specificity, sensitivity, repeatability and reproducibility for detection of anti-DPV antibodies in duck sera, and has the potential to be much simpler than DPV-ELISA and SNT for the sera epidemiological investigation.Duck plague (DP), which is caused by Duck Plague Virus (DPV), is an acute, contagious and lethal disease discovered, firstly, in Holland [1]. DPV is currently classified belonging to the Alphaherpesvirinae subfamily of the herpesvirus family but has not been grouped into any genus yet [2]. There are 34 species within Order Anseriformes' host range. Ducks, geese, and swans are the susceptive species to DP [3]. The characteristics of DP are vascular damage, tissue hemorrhages, digesti
Impact of the Location of CpG Methylation within the GSTP1 Gene on Its Specificity as a DNA Marker for Hepatocellular Carcinoma
Surbhi Jain, Sitong Chen, Kung-Chao Chang, Yih-Jyh Lin, Chi-Tan Hu, Batbold Boldbaatar, James P. Hamilton, Selena Y. Lin, Ting-Tsung Chang, Shun-Hua Chen, Wei Song, Stephen J. Meltzer, Timothy M. Block, Ying-Hsiu Su
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0035789
Abstract: Hypermethylation of the glutathione S-transferase π 1 (GSTP1) gene promoter region has been reported to be a potential biomarker to distinguish hepatocellular carcinoma (HCC) from other liver diseases. However, reports regarding how specific a marker it is have ranged from 100% to 0%. We hypothesized that, to a large extent, the variation of specificity depends on the location of the CpG sites analyzed. To test this hypothesis, we compared the methylation status of the GSTP1 promoter region of the DNA isolated from HCC, cirrhosis, hepatitis, and normal liver tissues by bisulfite–PCR sequencing. We found that the 5′ region of the position ?48 nt from the transcription start site of the GSTP1 gene is selectively methylated in HCC, whereas the 3′ region is methylated in all liver tissues examined, including normal liver and the HCC tissue. Interestingly, when DNA derived from fetal liver and 11 nonhepatic normal tissue was also examined by bisulfite-PCR sequencing, we found that methylation of the 3′ region of the promoter appeared to be liver-specific. A methylation-specific PCR assay targeting the 5′ region of the promoter was developed and used to quantify the methylated GSTP1 gene in various diseased liver tissues including HCC. When we used an assay targeting the 3′ region, we found that the methylation of the 5′-end of the GSTP1 promoter was significantly more specific than that of the 3′-end (97.1% vs. 60%, p<0.0001 by Fisher's exact test) for distinguishing HCC (n = 120) from hepatitis (n = 35) and cirrhosis (n = 35). Encouragingly, 33.8% of the AFP-negative HCC contained the methylated GSTP1 gene. This study clearly demonstrates the importance of the location of CpG site methylation for HCC specificity and how liver-specific DNA methylation should be considered when an epigenetic DNA marker is studied for detection of HCC.
Experimental study on fishery wastewater treatment using swim-bed anoxic and oxic process

Shi Lingyan Yang Fenglin Hu Shaowei Zhang Hanmin Liu Sitong,

环境工程学报 , 2009,
Abstract: 试验采用摇动床缺氧-好氧工艺处理渔业加工废水,研究结果表明,摇动床缺氧-好氧工艺对污染物去除效果良好,在BOD5容积负荷为1.5 kg/(m3·d),HRT=5.9~4.7 h,硝化液回流比为1.0的条件下,系统进水中COD、TN和NH+4-N的平均值分别为543.1、72.1和63.6 mg/L时,去除率分别达到93.6%、72.7%和98.9%,出水平均浓度值分别为34.1、19.5和0.7 mg/L,达到《城镇污水处理厂污染物排放标准》(GB 18918-2002) 一级标准。试验期间MLSS最高可达到17 425 mg/L,同时保持SVI在50~70 mL/g的低范围内,污泥沉降性能良好。通过显微镜观察,反应器中生物种类多样,从而保证了摇动床系统极低的污泥产率(MLSS/CODremoval为0.1891),实现了污泥减量。
SNAD for nitrogen removal using carbon tube membrane-aerated biofilm reactor

Wang Fang Yang Fenglin Gong Zheng Liu Sitong Hu Shaowei Lian Jianjun,

环境工程学报 , 2009,
Abstract: 以包裹无纺布的微孔炭管作为膜曝气生物膜反应器(MABR)的膜组件,进行了短程硝化,厌氧氨氧化和反硝化耦合脱氮(SNAD)研究。实验中,控制温度34±1℃,pH 7.5~8.5, HRT 8 h,通过逐步降低膜内压力使反应器中的溶解氧由8 mg/L逐步降低到0.5 mg/L以下。实验采用亚硝酸细菌挂膜,然后接种厌氧氨氧化细菌,实现在单一反应器中同时发生短程硝化、厌氧氨氧化和反硝化耦合脱氮功能。结果表明,经过180 d的连续稳定运行,氨氮去除率达到了93.4%,总氮去除率达到了92.5%,COD去除率达到97.2%, 氨氮去除负荷0.6 kg N/(m3 ·d)。适合SNAD工艺的最佳C/N比为0.2~0.6,当COD浓度过高时,会抑制厌氧氨氧化细菌,使SNAD工艺的处理效果明显下降。
WASD neural network activated by bipolar sigmoid functions together with subsequent iterations

ZHANG Yunong
,XIAO Zhengli,DING Sitong,MAO Mingzhi,LIU Jinrong

- , 2016,
Abstract: 结合Levenberg-Marquardt算法以及权值直接确定法这两种用于神经网络学习训练的方法,提出了一种带后续迭代、面向双极S (sigmoid)激励函数神经网络的权值与结构确定(weights-and-structure-determination, WASD)方法。该方法与MATLAB软件神经网络工具箱相结合,可以解决传统神经网络普遍存在的学习时间长、网络结构难以确定、学习能力和泛化能力有待提高等不足,同时具有较好的可行性和可操作性。以非线性函数的数据拟合为例,计算机数值实验和对比结果证实了WASD方法确定出最优隐神经元数和最优权值的优越性,最终得到的WASD神经网络具有更为优异的学习性能和泛化性能
Digital Image Watermarking Based on Mixed Error Correcting Code  [PDF]
Yonghong Chen, Jiancong Chen
Journal of Information Security (JIS) , 2012, DOI: 10.4236/jis.2012.32018
Abstract: In this paper, we present a novel technique based on a mixed Error Correcting Code(ECC)-the convolutional code and the repetition code to enhance the robustness of the embedded watermark. Before embedding, the binary watermark is scanned to one-dimension sequence and later inputted into the (3, 1, 2) convolutional encoder and (3, 1) repetition encoder frame by frame, which will improve the error correcting capability of decoder. The output code sequence is scanned to some matrixes as the new watermark messages. The watermarking is selected in low frequency band of the Discrete Wavelet Transform (DWT) and therefore it can resist the destruction of image processing. Experimental results are presented to demonstrate that the robustness of a watermark with mixed ECC is much higher than the traditional one just with repetition coding while suffering JPEG lossy compression, salt and pepper noise and center cutting processing.
Operator Equation and Application of Variation Iterative Method  [PDF]
Ning Chen, Jiqian Chen
Applied Mathematics (AM) , 2012, DOI: 10.4236/am.2012.38127
Abstract: In this paper, we study some semi-closed 1-set-contractive operators A and investigate the boundary conditions under which the topological degrees of 1-set contractive fields, deg (I-A, Ω, p) are equal to 1. Correspondingly, we can obtain some new fixed point theorems for 1-set-contractive operators which extend and improve many famous theorems such as the Leray-Schauder theorem, and operator equation, etc. Lemma 2.1 generalizes the famous theorem. The calculation of topological degrees and index are important things, which combine the existence of solution of for integration and differential equation and or approximation by iteration technique. So, we apply the effective modification of He’s variation iteration method to solve some nonlinear and linear equations are proceed to examine some a class of integral-differential equations, to illustrate the effectiveness and convenience of this method.
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