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Search Results: 1 - 10 of 2411 matches for " Silvana Creste "
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Sugarcane Transcript Profiling Assessed by cDNA-AFLP Analysis during the Interaction with Sugarcane Mosaic Virus  [PDF]
Cibele N. F. Medeiros, Marcos C. Gon?alves, Ricardo Harakava, Silvana Creste, Paula M. Nóbile, Luciana R. Pinto, Dilermando Perecin, Marcos G. A. Landell
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.49057

Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investigated in two sugarcane varieties contrasting to SCMV resistance, when challenged with a severe virus strain. Healthy plants derived from meristem tip tissue culture were mechanically inoculated under greenhouse controlled conditions and sampled at 24, 48 and 72 hours after inoculation. A total of 392 transcript-derived fragments (TDFs) were verified in the resistant variety against 380 in the susceptible one. The two sugarcane genotypes showed differential behavior in the number of induced and repressed TDFs along the time-course samplings. Ten out of 23 sequenced TDFs (unique from the resistance variety), showed identity with known plant sequences, mostly related to plant defense mechanisms against pathogens. The cDNA-AFLP technique was effective in revealing changes in the transcription profile within and between contrasting varieties when challenged by SCMV.

Boron fertilization on sweet potato: effect of sources, rates and application form Aduba o com boro em batata-doce: efeito das fontes, doses e modos de aplica o
Fábio Rafael Echer,José Eduardo Creste
Semina : Ciências Agrárias , 2011,
Abstract: Boron (B) fertilization can result in high yield and better sweet potato storage roots quality. However, there is few works about this subject. The objective was to evaluate the yield of sweet potato crops in response to B sources, rates and application form. The experimental design was complete randomized blocks, in factorial outline ((2x2x2)+1), with three replications, which resulted of the combination of sources of B (boric acid and borax), application form (via soil and via foliar) and doses of B (1 and 2 kg ha-1) and a control treatment (without B). There was no interaction between the factors studied for the commercial yield of sweet potato. No significant differences was found between B sources and application form, but they showed higher yield than the control treatment. Additionally, sweet potato yield increade with application of 2 kg ha-1 of B. A aduba o boratada pode proporcionar aumentos na produtividade e na qualidade das raízes tuberosas de batata-doce, no entanto poucos trabalhos contemplam esse assunto. Objetivou-se avaliar a produtividade da cultura da batata-doce em resposta a fontes, doses e modos de aplica o de B. O delineamento experimental utilizado foi o de blocos casualizados, em esquema fatorial ((2x2x2)+1), com três repeti es. Os tratamentos foram aplicados em cobertura, sendo resultados da combina o de fontes de B (ácido bórico e bórax), formas de aplica o (via solo e via foliar) e doses de B (1 e 2 kg ha-1) mais uma testemunha. N o houve intera o entre os fatores estudados sobre a produtividade comercial de batata-doce. As formas de aplica o, via solo e via foliar bem como as fontes ácido bórico e bórax n o apresentaram diferen as entre si, mas proporcionaram produtividades superiores ao tratamento sem B. A produtividade de batata-doce foi favorecida com a aplica o de 2 kg ha-1 de B.
A comunica??o da suspens?o de cirurgias pediátricas: sentimentos dos familiares envolvidos no processo
Risso, Amanda Creste Martins da Costa Ribeiro;Braga, Eliana Mara;
Revista da Escola de Enfermagem da USP , 2010, DOI: 10.1590/S0080-62342010000200017
Abstract: hospitalization is a complex experience; this can be made worse if surgical intervention is required, especially when the patient is a child. when surgery is suspended, patient and family can experience feelings of insecurity, distress, and anxiety. the objective of this study was to identify and describe the perceptions of fifteen mothers or guardians of children between 0 and 18 years old admitted in a teaching hospital, after receiving news that surgery for their child was suspended. this was a descriptive qualitative study which used interpersonal communication as theoretical reference and analysis of content as the methodology. results showed that suspension of pediatric surgery in our institution causes repercussions to patients, their families, and institution organisation; that communication between health team professionals, patients and families is inadequate; and that the nurses' participation in informing surgery suspension must be effective.
Simplifying Stochastic Mathematical Models of Biochemical Systems  [PDF]
Silvana Ilie, Samaneh Gholami
Applied Mathematics (AM) , 2013, DOI: 10.4236/am.2013.41A038

Stochastic modeling of biochemical reactions taking place at the cellular level has become the subject of intense research in recent years. Molecular interactions in a single cell exhibit random fluctuations. These fluctuations may be significant when small populations of some reacting species are present and then a stochastic description of the cellular dynamics is required. Often, the biochemically reacting systems encountered in applications consist of many species interacting through many reaction channels. Also, the dynamics of such systems is typically non-linear and presents multiple time-scales. Consequently, the stochastic mathematical models of biochemical systems can be quite complex and their analysis challenging. In this paper, we present a method to reduce a stochastic continuous model of well-stirred biochemical systems, the Chemical Langevin Equation, while preserving the overall behavior of the system. Several tests of our method on models of practical interest gave excellent results.

Automatic Simulation of the Chemical Langevin Equation  [PDF]
Silvana Ilie, Monjur Morshed
Applied Mathematics (AM) , 2013, DOI: 10.4236/am.2013.41A036

Biochemical systems have important practical applications, in particular to understanding critical intra-cellular processes. Often biochemical kinetic models represent cellular processes as systems of chemical reactions, traditionally modeled by the deterministic reaction rate equations. In the cellular environment, many biological processes are inherently stochastic. The stochastic fluctuations due to the presence of some low molecular populations may have a great impact on the biochemical system behavior. Then, stochastic models are required for an accurate description of the system dynamics. An important stochastic model of biochemical kinetics is the Chemical Langevin Equation. In this work, we provide a numerical method for approximating the solution of the Chemical Langevin Equation, namely the derivative-free Milstein scheme. The method is compared with the widely used strategy for this class of problems, the Milstein method. As opposed to the Milstein scheme, the proposed strategy has the advantage that it does not require the calculation of exact derivatives, while having the same strong order of accuracy as the Milstein scheme. Therefore it may be used for an automatic simulation of the numerical solution of the Chemical Langevin Equation. The tests on several models of practical interest show that our method performs very well.

Generational Change and the Military Dictatorship in Brazil: The Debate on the “AI-5 Generation”  [PDF]
Silvana Seabra Hooper
Creative Education (CE) , 2016, DOI: 10.4236/ce.2016.715226
Abstract: This article analyzes the issue of generational change, reviews its conceptual and historical origin in Mannheim and demonstrates the relationship between generational cohorts and traumatic historical moments. The paper focuses on a new generation in Brazil, the “AI-5 generation” (1970), which was identified by Luciano Martins (social scientist), and subsequently reconsidered by Jurandir Freire Costa (social psychanalyst). It discusses one possible reinterpretation of the concept within the context of the internationalization and modernization of that period in Brazil.
Acute Mesenteric Ischemia: A Challenging Diagnostic Disease—Four Cases Reports and Literature Review (AMI)  [PDF]
Danilo Coco, Silvana Leanza
Advances in Molecular Imaging (AMI) , 2018, DOI: 10.4236/ami.2018.84006
Abstract: Acute Mesenteric Ischemia (A.M.I.) is a potentially life-threatening condition syndrome due to inadequate or completely absent blood supply through superior or inferior mesenteric artery. The etiologies are various. Early diagnosis is essential to improve the clinical outcome. Despite advances in knowledge of pathophysiology, laboratory diagnosis and imaging techniques, acute intestinal ischemia is still associated with mortality rates.
In vitro growth of sweet potato fed with potassium phosphite
Hirosse, Edison Hitoshi;Creste, José Eduardo;Custódio, Ceci Castilho;Machado-Neto, Nelson Barbosa;
Acta Scientiarum. Agronomy , 2012, DOI: 10.4025/actasciagron.v34i1.10810
Abstract: the main objective of this work was to verify the effect of the substitution of phosphate by its related compound phosphite on the in vitro growth of sweet potato nodal segments. segments from a previous culture were incubated in ms media with the following combinations of phosphate:phosphite: control (100 / 0%); t1 (87.5 / 12.5%); t2 (75 / 25%); t3 (50 / 50%) and t4 (0 / 100%) in a 5 x 5 factorial arrangement, with five treatments (control, t1, t2, t3 and t4) and five evaluation periods (7, 14, 21, 28 and 35 days). the design was completely random, with three replicates of two explants per period. the evaluations were made weekly by counting the number of shoots and the length and dry weight of shoots and roots. increasing the phosphite concentration decreased all attributes measured, including growth velocity, length and dry weight. these results indicate that phosphite cannot be used as substituted of phosphate in sweet potato tissue cultures.
Fertiliza??o de cobertura com boro e potássio na nutri??o e produtividade da batata-doce
Echer, Fábio R;Dominato, Júlio C;Creste, José E;Santos, Diego H;
Horticultura Brasileira , 2009, DOI: 10.1590/S0102-05362009000200009
Abstract: most of brazilian soils have little or no mineral reserve of potassium and, usually low content of organic matter in the soil, the most important source of boron for the plants. in this work we evaluated the yield of sweet potato, cultivar canadense, in response to the combination of different rates of potassium fertilization and boron fertilization. the research was carried out from august 2007 to january 2008, in presidente prudente, s?o paulo state, brazil. the experimental design consisted of randomized blocks in a factorial scheme 4x4, with three repetitions: rates of potassium fertilization (0, 50, 100 and 200 kg ha-1 of k2o), and levels of boron fertilization (0, 1, 2 and 3 kg ha-1 of b), both in side dressing. the treatments were applied 62 days after planting date. the maximum estimated yield was of 27.7 t ha-1, with combination of the rates of 2 kg ha-1 of b allied to the application of 200 kg ha-1 of k2o. the foliar nutrient content of b increased with increasing rates of b and k. 1 and 3 kg ha-1 of b rates did not influence the foliar content of mg, but with rates of 0 and 2 kg ha-1 of b the mg foliar content presented quadratic behavior in function of increasing k fertilization.
Absor??o de nutrientes e distribui??o da massa fresca e seca entre órg?os de batata-doce
Echer, Fábio R;Dominato, Júlio C;Creste, José E;
Horticultura Brasileira , 2009, DOI: 10.1590/S0102-05362009000200010
Abstract: the absorption march studies the relationship between the amount of nutrients, dry mass accumulation and the plant age. the absorption march of nutrients, fresh mass and dry mass production of sweet potato organs was determined on the cultivar canadense. the experiment was carried out between august and december, 2007, in presidente prudente, s?o paulo state, brazil, on a commercial farm. the completely randomized experimental design was adopted, with three repetitions. the treatments consisted of eight collection dates, beginning 40 days after transplanting, at 15-day intervals. the storage roots provided higher production of fresh and dry mass at harvest in comparison to other organs. n and mn were the macro and micronutrients more absorbed by leaves, stems and storage roots of sweet potato, while higher absorption of ca and mn was observed in the roots. the period of larger absorption of n and k by storage roots was between 115-145 and 115-130 days after transplanting date, respectively.
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