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Search Results: 1 - 10 of 82799 matches for " Silvana Aparecida da Silva;Siqueira "
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Levantamento de moscas-das-frutas e seus parasitoides em citros, no município de Vi?osa, Minas Gerais
Machado, Daniel Lucas Magalh?es;Struiving, Tiago Barbosa;Santos, Dierlei dos;Souza, Silvana Aparecida da Silva;Siqueira, Dalmo Lopes de;
Revista Ceres , 2012, DOI: 10.1590/S0034-737X2012000600020
Abstract: fruit flies are responsible for large losses in commercial orchards in brazil, thus, it is important to know the predominant species in the region. the objective of this study was to study the occurrence of fruit flies (diptera: tephritoidae) and of their parasitoids in sweet orange (citrus sinensis l. osbeck), 'ponc?' mandarin (citrus reticulate blanco) and 'rio' tangerine (citrus deliciosa ten), in vi?osa, minas gerais. the fruits were collected in april 2008. in the laboratory, the fruits were stored in plastic boxes containing moist sand in a controlled environment, to obtain pupae. then, the pupae were counted, placed in glass bottles with fine sand and kept in an oven until adult emergence. only one species of fruit fly (anastrepha fraterculus (wiedemann, 1830) and one species of parasitoid (doryctobracon brasiliensis szépligeti) were identified. among the varieties, the sweet orange 'baianinha' showed the highest rate of infection and the lower were assigned to the 'rio' tangerine and 'ponc?' mandarin.
Viscum Album Modulates Apoptotic Related Genes in Melanoma Tumor of Mice  [PDF]
Anamaria da Silva Facina, Gil Facina, Ismael Dale Cotrim Guerreiro da Silva, Giovana Aparecida Gon?alves, Fernando Augusto de Almeida, Silvana Aparecida Alves Corrêa de Noronha, Samuel Marcos Ribeiro de Noronha, Mary Uchiyama Nakamura
American Journal of Molecular Biology (AJMB) , 2014, DOI: 10.4236/ajmb.2014.42007
Abstract:

Cancer is a major public health problem throughout the world. It is estimated that one third of the American population will develop the disease at some time during their lifetimes. Among these, melanoma will account for 7% of the cases. In Brazil, in 2012, it is estimated that over six thousand new melanoma cases occurred. During recent years, the incidence of melanoma has increased, mainly due to a more constant exposure of the skin to sunlight. In this work, our aim is to assess the expression of apoptotis-related genes melanoma tumors in mice treated with Viscum album (VA). This will allow us to better understand the molecular mechanisms underlying tumor cell death activation caused by this compound. Our results clearly demonstrate upregulation of pro apoptotic genes (Trp53bp2, Nol3, Fadd, Tnfsf10, Traf1, Traf2, Cflar, Card10, Nod1, Casp 2, Casp7, Xiap, Dad1, and Dffb). Further bioinformatics-based tool analysis allowed us to assess which specific cell death-related intracellular pathways were activated by VA treatment. Two major effects of VA in melanoma cells could be observed: generation of

Leptin Signaling Modulates Expression of Polycomb and Trithorax Complexes in the Brain of Fat Tissue Implanted Polycystic Ovarian Sindrome Mice  [PDF]
Eduardo Henrique da Silva Freitas, Samuel Marcos Ribeiro de Noronha, Maria Nazareth Gamboa Ritto, Carlos Fernandes Baptista, Ismael Dale Cotrim Guerreiro da Silva, Silvana Aparecida Alves Correa-Noronha, Ivaldo da Silva
American Journal of Molecular Biology (AJMB) , 2014, DOI: 10.4236/ajmb.2014.44020
Abstract: The Polycystic Ovary Syndrome (PCOS) is the most common androgenic disorder in women during reproductive life. PCOS may also be accompanied by metabolic syndrome and recent studies point to leptin as playing a role in disrupting infertility and in changing the energy balance in obese mice through its action on the hypothalamus. The aim is to assess the expression of the Polycomb & Trithorax Complexes genes in brain of mice transplanted with fat tissue from normal mice, in order to better understand the neuronal mechanisms underlying the reversion of PCOS. Three B6 V-Lepob/J mouse groups: Normal weight, obese and seven-day-treatment obese had their brain RNA extracted and submitted to an 84 Polycomb & Trithorax Complexes genes PCR Array plate and MetacoreTM pathways localization. Genomic profiles obtained were compared to the ones of the normal-weight-mice group. Differentially expressed genes were 13% and 26% respectively to control and treatment. Major changes were in genes: Snai1/31; Smarca1/?17; Dnmt3b/4.7; Ezh1/ 15. Altered genes were associated to canonical pathways and provided 3 networks related to epigenetics. Underlying neuronal changes caused by leptin in obese mice brain, there is an important role being played by the histone code. Here there is evidence that leptin drives the chromatin packing to a more condensed pattern. Upregulation of methyltransferase genes, like Ezh1, favors this thought. In summary the Polycomb & Trithorax complexes might answer for the silencing of some downregulated genes in the obese mice brain when exposed to leptin.
Fungos micorrízicos no crescimento e na extra??o de metais pesados pela braquiária em solo contaminado
Silva, Silvana da;Siqueira, José Oswaldo;Soares, Cláudio Roberto Fonsêca Sousa;
Pesquisa Agropecuária Brasileira , 2006, DOI: 10.1590/S0100-204X2006001200009
Abstract: the influence of 14 arbuscular mycorrhiza fungal isolates (amf) on brachiariagrass growth and extraction of zn, cd, cu and pb from a contaminated soil was evaluated in a greenhouse experiment. mycorrhizal and nonmycorrhizal brachiaria decumbens plants were grown in pots with 0.92 kg of soil containing (mg kg-1): zn, 3,300; cu, 60; cd, 29; pb, 73. the amf increased dry matter yield in 84%. plant heavy metal concentrations were high and were inversely related to dry matter yield. isolates caused no effect on pb, but reduced shoot zn, cd and cu concentrations by 20, 28 and 63%, respectively. it was observed effect of mycorrhiza on cu accumulation in roots (over 1,000%). isolates enhanced the total amount of soil extracted metals by 845, 142, 68 and 54% for cu, pb, zn and cd, respectively. only acaulospora spinosa, acaulospora morrowiae-ufla and gigaspora gigantea enhanced simultaneous soil extraction of all the four metals. the amf increase the capacity of b. decumbens to extract contaminant heavy metals from soil, with differentiated effects amongst species.
Angiotensin-(1-7) Changes Apoptosis-Related Genes Expression in Human Breast Cancer Cell Line T47D  [PDF]
Cheryl Alecrim Santos, Gabriela Soares da Silva Brito, Silvana Aparecida Alves Corrêa de Noronha, Samuel Marcos Ribeiro de Noronha, Suma Imura Shimuta, Clovis Ryiuchi Nakaie, Ismael Dale Cotrim Guerreiro da Silva
Journal of Cancer Therapy (JCT) , 2014, DOI: 10.4236/jct.2014.514143
Abstract: Angiotensin-(1-7) [Ang-(1-7)] is a heptapeptide of the renin-angiotensin system with vasodilator and anti-proliferative properties. In the present study, we aim to investigate whether Ang-(1-7) induces apoptosis in breast cancer cells and whether the altered expression of apoptosis-related genes is involved in this process. Human breast cell line T47D was treated with angiotensin-(1-7) and angiotensin II (Ang II). Cell proliferation and apoptosis were quantified using hemocytometer and flow cytometry, respectively. The expression of 84 apoptosis-related genes was evaluated through qPCR array. Ang-(1-7), as opposed to Ang II, decreased proliferation and increased apoptosis in T47D cells. Moreover, many pro-apoptotic genes were up-regulated, such as BAK1, BAX, BCL2L1, BID and BIK.
Diagnóstico de resistência do Mycobacterium tuberculosis à rifampicina utilizando-se da rea??o em cadeia da polimerase
Carvalho, Wania da Silva;Miranda, Silvana Spíndola de;Pesquero, Jorge Luiz;Gomes, Maria Aparecida;
Revista Brasileira de Ciências Farmacêuticas , 2007, DOI: 10.1590/S1516-93322007000100004
Abstract: the resistance of mycobacterium tuberculosis to tuberculostatic drugs has emerged as a major public health threat. the resistance to rifampicin which has been attributed to structural changes in rna polymerase can be considered as a marker for multi-drug-resistance to tuberculosis (mdr-tb). patients bearing rifampicin-resistant strains have poor diagnosis even with treatment. conventional culture-based drug sensibility testing can require several weeks due to the growth. in this paper we describe the most common pcr-based methods for detection of mutations that lead to rifampicin resistance, such as single-strand conformation polymorphism (sscp), pcr heteroduplex and inno-lipa. recently, by low stringency using a single specific primer (lssp) assay, it was standardized a protocol that showed to be rapid and sensitive for the detection of mutations in the rpob gene.
Levantamento de moscas-das-frutas e seus parasitoides em citros, no município de Vi osa, Minas Gerais Survey of fruit-flies and their parasitoids in citrus in Vi osa, Minas Gerais
Daniel Lucas Magalh?es Machado,Tiago Barbosa Struiving,Dierlei dos Santos,Silvana Aparecida da Silva Souza
Revista Ceres , 2012,
Abstract: As moscas-das-frutas s o responsáveis por grandes perdas em fruteiras comerciais no Brasil, por isso é fundamental conhecer as espécies predominantes na regi o. Objetivou-se com este trabalho estudar a ocorrência de moscas-das-frutas (Diptera: Tephritidae) e seus parasitoides em laranjas doces (Citrus sinensis L. Osbeck), tangerina Ponc (Citrus reticulata Blanco) e mexerica Rio (Citrus deliciosa Ten), no município de Vi osa, Minas Gerais. Os frutos foram coletados em abril de 2008. No laboratório eles foram acondicionados em caixas plásticas contendo areia umedecida e em ambiente controlado para obten o dos pupários, que foram contados, acondicionados em frascos de vidro com areia fina e mantidos em estufa até a emergência dos adultos. Somente uma espécie de mosca-das-frutas (Anastrepha fraterculus (Wiedemann, 1830) e uma de parasitoide (Doryctobracon brasiliensis Szépligeti) foram identificadas. Dentre as variedades, a laranja doce Baianinha apresentou o maior índice de infesta o, e os menores foram atribuídos à mexerica Rio e à tangerina Ponc . Fruit flies are responsible for large losses in commercial orchards in Brazil, thus, it is important to know the predominant species in the region. The objective of this study was to study the occurrence of fruit flies (Diptera: Tephritoidae) and of their parasitoids in sweet orange (Citrus sinensis L. Osbeck), 'Ponc ' mandarin (Citrus reticulate Blanco) and 'Rio' tangerine (Citrus deliciosa Ten), in Vi osa, Minas Gerais. The fruits were collected in April 2008. In the laboratory, the fruits were stored in plastic boxes containing moist sand in a controlled environment, to obtain pupae. Then, the pupae were counted, placed in glass bottles with fine sand and kept in an oven until adult emergence. Only one species of fruit fly (Anastrepha fraterculus (Wiedemann, 1830) and one species of parasitoid (Doryctobracon brasiliensis Szépligeti) were identified. Among the varieties, the sweet orange 'Baianinha' showed the highest rate of infection and the lower were assigned to the 'Rio' tangerine and 'Ponc ' mandarin.
Hiperplasia adrenal congênita: deficiência de 17alfa-hidroxilase
Silva, Eliana Aparecida da;Siqueira, Ana Carla Lobo;Pacheco, Suzana;Ribeiro, Alexandre Kazantzi;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2000, DOI: 10.1590/S0004-27302000000200012
Abstract: the authors present a case of 17a-hydroxylase deficiency, a rare enzymatic defect responsible for c.a.h. biglieri and cols. published in 1966 the first report of this enzymatic defect. today there are 180 cases reported in the literature. the patient being reported showed primary amenorrhea, absence of secondary sexual characteristics and hypertension of difficult control. during investigation a 46xy kariotype was found together with renin suppression, low androstenedione and testosterone and elevated pregnenolone levels. the patient was submitted to bilateral orchiectomy and is being clinically accompanied with conjugated estrogens, spironolactone, beta-blockers and dexamethasone while waiting for vaginoplasty.
Avalia??o da aten??o à saúde da crian?a (0-5 anos) no PSF de Teresópolis (RJ) segundo a percep??o dos usuários
Ribeiro,José Mendes; Siqueira,Sandra Aparecida Venancio de; Pinto,Luiz Felipe da Silva;
Ciência & Saúde Coletiva , 2010, DOI: 10.1590/S1413-81232010000200028
Abstract: family health strategy represents one of the major public programs in brazil covering 5,601 municipalities in 2006 (91.8%). this study evaluated teresópolis family health strategy through information and perceptions of adults responsible for children around 0-5 years that used those health services. it was used evaluation forms answered by family in waiting rooms or during home visiting. results for urban and rural areas were compared and significant differences were observed in some issues. major findings include users' strong ties with the program and a highly positive perception about the children health status. access was satisfactory as to use criteria when relating to regular care proceedings. critical points observed include: low coverage of home visiting, bad access to labs and image exams and to systematic delivery of medicines. rural areas presented a high number of children who treated acute clinical conditions at home without doctor assistance. users satisfaction as to physicians, nurses, health community agents and services were high level. users not regularly visited at home presented lower access to medicine delivery.
The Education for the Stone “: some glances on the artistic language in the youths and adults′ litteracy
Regina Aparecida Ribeiro Siqueira,Janaína Behling,Luiza Chu,Viviane Ramos da Silva
Educa??o : Teoria e Prática , 2009,
Abstract: This paper presents some reflections of young and adult educators when situated, themselves, as subjects of their actions in a Painting Workshop. We analyzed an experience produced by PEJA - Projeto de Educa o de Jovens e Adultos (Young and Adult Education Project) – Unesp/Assis. The educators of this project offered a Workshop entitled “Arts and Education: the arts as language in the education of youngsters and adults”, destinated to educators of the City Board of Education Assis (SP) and to undergraduate students of the University. Each one of the participants was invited to make a picture, using various materials as the conventional brushes and paints, besides seeds, leaves, stones and a lot of sensibility to discuss pedagogical theory and practice. The Workshop took place in 2003, because of the V Education Journey of Unesp, with a total time of 8 hours.
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