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Search Results: 1 - 10 of 20335 matches for " Sijia Min "
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Effects of Human Insulin Gene Transfection on the Adipogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells in Silk Fibroin Scaffolds in Vitro  [PDF]
Cheng Zhang, Yi Liu, Jun Tang, Meisi Xue, Sijia Min
Open Journal of Regenerative Medicine (OJRM) , 2015, DOI: 10.4236/ojrm.2015.42003
Abstract: The resorption of the transplanted fat over time limited the use of autologous fat for the reconstruction of soft tissue defect. Tissue engineering (TE) adipose with silk fibroin scaffold could be a promising substitute for soft tissue filling. In this study, we try to develop a tissue engineering adipose in vitro by seeding silk fibroin scaffold with human umbilical cord mesenchymal stem cells (hUCMSCs) after transfected with recombinant human insulin gene lentivirus. Our aim was to observe the effects of the insulin gene transfection on the adipogenesis of hUCMSCs when cultured with silk fibroin scaffolds. The hUCMSCs infected with recombinant lentiviral pLenti6.3-insulin-IRES-EGFP were seeded on silk fibroin scaffolds and cultured in adipogenic differentiation medium for 5 - 7 days. The expression of adipogenic gene PPARγ-2 was tested by RT-PCR after 7 days culture of adipogenic induction. The accumulation of cytoplasmic droplets of neutral lipids was assessed by Oil Red O staining. The RNA and protein expression of transfected insulin gene in hUCMSCs were detected by QPCR and western blot. The effect of recombinant lentivirus transfection on the growth and proliferation of hUCMSCs was observed by MTT test. We observed that the 2-ΔΔCt value of insulin gene expression of hUCMSCs in the transfected group was 300.25 times higher than that in the untransfected group. The western blot showed that a positive band was discerned at the site of a relative molecular mass of 8 × 103 Dalton in transfected group. After adipogenic culture for 7 days, under the fluorescent inverted phase-contrast microscope, after Oil Red O staining, a lot of adipocytes appeared in silk fibroin scaffold; round adipose droplets showed intracellularly; the size of the adipocytes was not homogenous, and the density of adipocytes in transfected group was significantly higher than that in untransfected group (P = 0.007, P < 0.01). RT-PCR results showed that the expression of adipogenic gene PPARγ-2 in transfected group was much stronger than that in untransfected group. MTT test showed that there was no significant difference in optical density (A) at each time point between transfected group and nontransfected group (P = 0.056, P > 0.05). And there was also no significant difference in optical density (A) between cell group and cell-scalffold group (P = 0.066, P > 0.05). We concluded that insulin gene could obviously promote the adipogenic differentiation of hUCMSCs, and a tissue engineering adipose could be constructed
Preparation of Porous Scaffolds from Silk Fibroin Extracted from the Silk Gland of Bombyx mori (B. mori)
Mingying Yang,Yajun Shuai,Wen He,Sijia Min,Liangjun Zhu
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms13067762
Abstract: In order to use a simple and ecofriendly method to prepare porous silk scaffolds, aqueous silk fibroin solution (ASF) was extracted from silk gland of 7-day-old fifth instar larvae of Bombyx mori ( B. mori). SDS-page analysis indicated that the obtained fibroin had a molecular weight higher than 200 kDa. The fabrication of porous scaffolds from ASF was achieved by using the freeze-drying method. The pore of porous scaffolds is homogenous and tends to become smaller with an increase in the concentration of ASF. Conversely, the porosity is decreased. The porous scaffolds show impressive compressive strength which can be as high as 6.9 ± 0.4 MPa. Furthermore, ASF has high cell adhesion and growth activity. It also exhibits high ALP activity. This implies that porous scaffolds prepared from ASF have biocompatibility. Therefore, the porous scaffolds prepared in this study have potential application in tissue engineering due to the impressive compressive strength and?biocompatibility.
Supply Chain Decision Analysis Based on Risk-Aversion and Cost Information Asymmetry  [PDF]
Sijia Xiong, Liyang Xiong
Journal of Service Science and Management (JSSM) , 2019, DOI: 10.4236/jssm.2019.123030
Abstract: This paper analyzes the impact of risk aversion and retailer’s cost lied factor on supply chain decision-making in a hybrid channel supply chain system consisting of a risk-averse manufacturer and a retailer. According to the expected utility maximization theory, this paper gets the optimal decision of the manufacturer and retailer. First, it analyzes the impact of risk aversion factor on pricing decisions of supply chain members when the information of the retailer’s cost is public. Next, this paper considers the fact that the retailer has advantages on cost information, analyzes the relations between the retailer’s optimal cost lied factor and each parameter.
Enhancing Effect of Glycerol on the Tensile Properties of Bombyx mori Cocoon Sericin Films
Haiping Zhang,Lianxia Deng,Mingying Yang,Sijia Min,Lei Yang,Liangjun Zhu
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12053170
Abstract: An environmental physical method described herein was developed to improve the tensile properties of Bombyx mori cocoon sericin films, by using the plasticizer of glycerol, which has a nontoxic effect compared with other chemical crosslinkers. The changes in the tensile characteristics and the structure of glycerolated (0–40 wt% of glycerol) sericin films were investigated. Sericin films, both in dry and wet states, showed enhanced tensile properties, which might be regulated by the addition of different concentrations of glycerol. The introduction of glycerol results in the higher amorphous structure in sericin films as evidenced by analysis of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectra, thermogravimetry (TGA) and differential scanning calorimetry (DSC) curves. Scanning Electron Microscopy (SEM) observation revealed that glycerol was homogeneously blended with sericin molecules when its content was 10 wt%, while a small amount of redundant glycerol emerged on the surface of sericin films when its content was increased to 20 wt% or higher. Our results suggest that the introduction of glycerol is a novel nontoxic strategy which can improve the mechanical features of sericin-based materials and subsequently promote the feasibility of its application in tissue?engineering.
Teachers’ Teaching Communicative Rationality: Connotation, Mechanisms and Strategies
Sijia Liu,Yu Gao
Asian Social Science , 2011, DOI: 10.5539/ass.v7n3p117
Abstract: It is a universal phenomenon that there has been lack of communicative rationality in teachers’ classroom teaching communication. The essence of teaching communicative rationality is regularity, purposiveness and normativity. Besides those, the formation of teaching communicative rationality is restricted by the thinking mode, experience, habits, commonsense, sensibilities and other passive cultural factors of instrumental rationalism, as well as the dehumanized classroom communicative norms and the teaching behavioral patterns of giving and accepting. The generating mechanisms of teachers’ teaching communicative rationality are as follows. The core factor is reflective communication practice. The intrinsic power is the communication motivation of self-fulfillment. The rational use of languages comes to the necessary condition. The essential guarantee is the humanized communication norm. In view of what has been mentioned above, it is necessary to take measures to promote teachers’ communicative rationality from four aspects: reconstructing the value rationality of teaching communication, re-understanding the meaning of teaching communication, initiatively promoting the individual construction and consciously reflecting the practice of inner communication.
L^2-concentration phenomenon for Zakharov system below energy norm
Daoyuan Fang,Sijia Zhong
Mathematics , 2007,
Abstract: In this paper, we'll prove a L^2-concentration result of Zakharov system in space dimension two, with radial initial data (u_0,n_0,n_1)\in H^s\times L^2\times H^{-1} ({16/17}
Research on Traveling Routes Problems Based on Improved Ant Colony Algorithm  [PDF]
Zhanchang Yu, Sijia Zhang, Siyong Chen, Bingxing Liu, Shiqi Ye
Communications and Network (CN) , 2013, DOI: 10.4236/cn.2013.53B2109
Abstract:

This paper studies how to obtain a reasonable traveling route among given attractions. Toward this purpose, we propose an objective optimization model of routes choosing, which is based on the improved Ant Colony Algorithm. Furthermore, we make some adjustment in parameters in order to improve the precision of this algorithm. For example, the inspired factor has been changed to get better results. Also, the ways of searching have been adjusted so that the traveling routes will be well designed to achieve optimal effects. At last, we select a series of attractions in Beijing as data to do an experimental analysis, which comes out with an optimum route arrangement for the travelers; that is to say, the models we propose and the algorithm we improved are reasonable and effective.

73 GHz Wideband Millimeter-Wave Foliage and Ground Reflection Measurements and Models
Theodore S. Rappaport,Sijia Deng
Mathematics , 2015,
Abstract: This paper presents 73 GHz wideband outdoor foliage and ground reflection measurements. Propagation measurements were made with a 400 Megachip-per-second sliding correlator channel sounder, with rotatable 27 dBi (7 degrees half- power beamwidth) horn antennas at both the transmitter and receiver, to study foliage-induced scattering and de-polarization effects, to assist in developing future wireless systems that will use adaptive array antennas. Signal attenuation through foliage was measured to be 0.4 dB/m for both co- and cross-polarized antenna configurations. Measured ground reflection coefficients for dirt and gravel ranged from 0.02 to 0.34, for incident angles ranging from 60 degrees to 81 degrees (with respect to the normal incidence of the surface). These data are useful for link budget design and site-specific (ray-tracing) models for future millimeter-wave communication systems.
Adaptive Detection in Compound-Gaussian Clutter with Inverse Gaussian Texture
Sijia Chen;Lingjiang Kong;Jianyu Yang
PIER M , 2013, DOI: 10.2528/PIERM12121209
Abstract: This paper mainly deals with the detection problem of the target in the presence of the Compound-Gaussian (CG) distribution clutter with the unknown Power Spectral Density (PSD). Traditionally, the CG distributions, in particular the K distribution and the complex multivariate distribution, are the widely used models for the clutter measurements from the High Resolution (HR) radars. Recently, the CG distribution with the Inverse Gaussian (IG) texture, the specific class of CG clutter, is represented as the IG-CG distribution and validated to provide the better fit with the recorded clutter data than the mentioned two competitors. Within the IG-CG framework, the detector is here proposed in terms of the two-step Generalized Likelihood Ratio Test (GLRT) criterion, and the empirical estimation method is resorted to estimate the unknown PSD in order to adapt the realistic scenario. The proposed detector is tested on the real-life IPIX radar data, in comparison with the existing Adaptive Normalized Matched Filter (ANMF) processor, and the detection results illustrate that it outperforms ANMF.
Small Target Detection in Heavy Sea Clutter
Sijia Chen;Lingjiang Kong;Jianyu Yang
PIER B , 2012, DOI: 10.2528/PIERB12072111
Abstract: This paper mainly deals with the detection problem of the target with low Radar Cross-Section (RCS) in heavy sea clutter with unknown Power Spectral Density (PSD). Since the performance of traditional singlescan detectors degrades as the target of interest is smaller and weaker, three adaptive detectors, based upon a two-step design procedure, are proposed within the framework of the multiple-scan signal model. Firstly, the Multiple-Scan Detectors (MSDs) are derived according to the Generalized Likelihood Ratio Test (GLRT), Rao and Wald tests respectively under the assumption that the PSD of primary data is known. Secondly, three strategies are resorted to estimate the unknown PSD, and their Constant False Alarm Rate (CFAR) properties are assessed. Finally, numerical simulation results show that the adaptive MSDs outperform the traditional single-scan detector using Monte Carlo method.
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