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Search Results: 1 - 10 of 127051 matches for " Shuyu Li "
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The Rice Semi-Dwarf Mutant sd37, Caused by a Mutation in CYP96B4, Plays an Important Role in the Fine-Tuning of Plant Growth
Jie Zhang, Xiaoqiang Liu, Shuyu Li, Zhukuan Cheng, Chuanyou Li
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0088068
Abstract: Plant cytochrome P450 has diverse roles in developmental processes and in the response to environmental cues. Here, we characterized the rice (Oryza sativa L ssp. indica cultivar 3037) semi-dwarf mutant sd37, in which the gene CYP96B4 (Cytochrome P450 96B subfamily) was identified and confirmed as the target by map-based cloning and a complementation test. A point mutation in the SRS2 domain of CYP96B4 resulted in a threonine to lysine substitution in the sd37 mutant. Examination of the subcellular localization of the protein revealed that SD37 was ER-localized protein. And SD37 was predominantly expressed in the shoot apical meristem and developing leaf and root maturation zone but not in the root apical meristem. The sd37 leaves, panicles, and seeds were smaller than those of the wild type. Histological analysis further revealed that a decrease in cell number in the mutant, specifically in the shoots, was the main cause of the dwarf phenotype. Microarray analysis demonstrated that the expression of several cell division-related genes was disturbed in the sd37 mutant. In addition, mutation or strongly overexpression of SD37 results in dwarf plants but moderate overexpression increases plant height. These data suggest that CYP96B4 may be an important regulator of plant growth that affects plant height in rice.
A Guess Model of Black Holes and the Evolution of Universe  [PDF]
Zhenhua Mei, Shuyu Mei
Journal of Modern Physics (JMP) , 2012, DOI: 10.4236/jmp.2012.329154
Abstract: Based on the gravitational theory, fundamental data, and comprehensible suppositions, an evolution model of the universe was proposed. The universe exists in explosion and constringency mobile equilibrium state. The critical sizes of celestial bodies were calculated in their evolution process.
Solvent Effects on the UV Absorption Spectrum of Carmofur  [PDF]
Peipei Zhang, Shuyu Liu
Detection (Detection) , 2016, DOI: 10.4236/detection.2016.41004
Abstract: In this article, we reported that carmofur could be induced by some solvent to produce conformational alteration. Ultraviolet (UV) spectra were used to study the conformation alteration of carmofur. Upon the addition of acid in the some solvent, UV spectroscopy of carmofur could change gradually. When base was added to this system, UV spectroscopy of carmofur could return to the original state, and the change process was reversible. The variable temperature 1H and 13C-NMR spectrum were used to testify that temperature did not have any effect on the conformation alteration of carmofur in Acetonitrile: Trifluoroacetic-acid (9:1). These two conformers of carmofur were structurally stable in Acetonitrile: Trifluoroacetic-acid (9:1).
Mass the Confined Movement of Energy  [PDF]
Zhenhua Mei, Shuyu Mei
Journal of Modern Physics (JMP) , 2017, DOI: 10.4236/jmp.2017.86058
Abstract: According to Einstein’s mass-energy relation (E=mc2), and the discovery of neutrino oscillation fact, a new view of mass nature is proposed: The rest mass of a particle is not else something but just the confined movement of energy.
Gene Features Selection for Three-Class Disease Classification via Multiple Orthogonal Partial Least Square Discriminant Analysis and S-Plot Using Microarray Data
Mingxing Yang, Xiumin Li, Zhibin Li, Zhimin Ou, Ming Liu, Suhuan Liu, Xuejun Li, Shuyu Yang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0084253
Abstract: Motivation DNA microarray analysis is characterized by obtaining a large number of gene variables from a small number of observations. Cluster analysis is widely used to analyze DNA microarray data to make classification and diagnosis of disease. Because there are so many irrelevant and insignificant genes in a dataset, a feature selection approach must be employed in data analysis. The performance of cluster analysis of this high-throughput data depends on whether the feature selection approach chooses the most relevant genes associated with disease classes. Results Here we proposed a new method using multiple Orthogonal Partial Least Squares-Discriminant Analysis (mOPLS-DA) models and S-plots to select the most relevant genes to conduct three-class disease classification and prediction. We tested our method using Golub’s leukemia microarray data. For three classes with subtypes, we proposed hierarchical orthogonal partial least squares-discriminant analysis (OPLS-DA) models and S-plots to select features for two main classes and their subtypes. For three classes in parallel, we employed three OPLS-DA models and S-plots to choose marker genes for each class. The power of feature selection to classify and predict three-class disease was evaluated using cluster analysis. Further, the general performance of our method was tested using four public datasets and compared with those of four other feature selection methods. The results revealed that our method effectively selected the most relevant features for disease classification and prediction, and its performance was better than that of the other methods.
重建帝國﹕歷史正劇與中國當代民族主義在大眾文化中的新的表現形式 (Rebuilding the Empire: Historical TV Drama and the New Expressive Form of Cultural Nationalism)
Shuyu Kong
PORTAL : Journal of Multidisciplinary International Studies , 2007,
Abstract: This paper explores the discourse of cultural nationalism and its recent articulation in historical TV dramas (Lishi ju): TV serials set in the Chinese imperial past and depicting court politics and the private lives of imperial families. First, I briefly survey the recent resurgence of historical drama on the TV screen, especially comparing two different ways of representing history: “history light” (xishuo) and “history orthodox” (zhengju). While history light, a new genre strongly influenced by the costume dramas imported from Hong Kong and Taiwan, emphasizes the entertainment values of popular culture and adopts a postmodern attitude towards history, history orthodox renews the pedagogical tradition and the moralistic narrative of historical drama in modern China since the May Fourth enlightenment movement. I then focus on TV dramas in the history orthodox mode and their ideological messages, examining two representative works by Hu Mei: Yongzheng Dynasty (Yongzheng wangchao, 1999) and The Great Emperor Wu of Han (Hanwu dadi, 2005). While drawing attention to the various narrative strategies, intertextualities and audio-visual styles employed in these dramas to represent the glorious national history and portray a strong leader (the emperor) as national hero, I also provide a contextual analysis of the production and circulation of these two dramas as well as the critical and media response to them, to reveal the social agencies and social formation of these dramas behind the screen. I suggest that the revisionist reframing of the past in historical TV drama reflects a new nationalist historical consciousness and cultural identity borne out of China’s rapid rise and aspirations to become an economic and political superpower.
Surface Morphology of Amygdala Is Associated with Trait Anxiety
Shuyu Li, Yanan Wang, Pengfei Xu, Fang Pu, Deyu Li, Yubo Fan, Gaolang Gong, Yuejia Luo
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0047817
Abstract: Previous neuroimaging studies have suggested a role of amygdala in trait anxiety level, in which amygdala was typically treated as a whole. To date, it remains unknown whether the morphology of specific subregions of amygdala are associated with trait anxiety. Here, we employed a shape analysis approach to locate the association between its morphology and trait anxiety on the surface of amygdala. 24 healthy young participants were included. The boundary of amygdala for each subject was first manually outlined using high-resolution magnetic resonance (MR) image, followed by 3D surface reconstruction and parameterization using spherical harmonic description. Two point-wise metrics, direct displacement between the individual surface and atlas surface and its normal projection, were used to quantify the surface morphology of amygdala. Statistical analysis revealed significant correlations between the two surface metrics and trait anxiety levels, which were located around the lateral and central nucleus of right amygdala. Our results provided localized information for the association between amygdala and trait anxiety, and suggested a central role of the lateral and central nucleus of right amygdala on trait anxiety.
Too much data, but little inter-changeability: a lesson learned from mining public data on tissue specificity of gene expression
Shuyu Li, Yiqun Li, Tao Wei, Eric Su, Kevin Duffin, Birong Liao
Biology Direct , 2006, DOI: 10.1186/1745-6150-1-33
Abstract: There are 42–54% of genes showing significant correlations in tissue expression patterns between SAGE and GeneChip, with 30–40% of genes whose expression patterns are positively correlated and 10–15% of genes whose expression patterns are negatively correlated at a statistically significant level (p = 0.05). Our analysis suggests that the discrepancy on the expression patterns derived from technology platforms is not likely from the heterogeneity of tissues used in these technologies, or other spurious correlations resulting from microarray probe design, abundance of genes, or gene function. The discrepancy can be partially explained by errors in the original assignment of SAGE tags to genes due to the evolution of sequence databases. In addition, sequence analysis has indicated that many SAGE tags and Affymetrix array probe sets are mapped to different splice variants or different sequence regions although they represent the same gene, which also contributes to the observed discrepancies between SAGE and array expression data.To our knowledge, this is the first report attempting to mine gene expression patterns across tissues using public data from different technology platforms. Unlike previous similar studies that only demonstrated the discrepancies between the two gene expression platforms, we carried out in-depth analysis to further investigate the cause for such discrepancies. Our study shows that the exploitation of rich public expression resource requires extensive knowledge about the technologies, and experiment. Informatic methodologies for better interoperability among platforms still remain a gap. One of the areas that can be improved practically is the accurate sequence mapping of SAGE tags and array probes to full-length genes.This article was reviewed by Dr. I. King Jordan, Dr. Joel Bader, and Dr. Arcady Mushegian.Reviewed by Dr. I. King Jordan, Dr. Joel Bader, and Dr. Arcady Mushegian. For the full reviews, please go to the Reviewers' comments sectio
Cytosolic Entry of Shiga-Like Toxin A Chain from the Yeast Endoplasmic Reticulum Requires Catalytically Active Hrd1p
Shuyu Li, Robert A. Spooner, Randolph Y. Hampton, J. Michael Lord, Lynne M. Roberts
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041119
Abstract: Background Escherichia coli Shiga-like toxin 1 normally traffics to the endoplasmic reticulum (ER) in sensitive mammalian cells from where the catalytic A chain (SLTxA1) dislocates to the cytosol to inactivate ribosomes. Currently, no molecular details of the dislocation process are available. To investigate the mechanism of the dislocation step we expressed SLTxA1 in the ER of Saccharomyces cerevisiae. Methodology and Principal Findings Using a combination of growth studies and biochemical tracking in yeast knock-out strains we show that SLTxA1 follows an ER-associated degradation (ERAD) pathway to enter the cytosol in a step mediated by the transmembrane Hrd1p ubiquitin ligase complex. ER-to-cytosol dislocation of the bulk population of SLTxA1 requires Cdc48p and its ubiquitin-handling co-factor Npl4p, and this population of toxin is terminally dispatched by proteasomal degradation. A small sub-population of SLTxA1 uncouples from this classical ERAD pathway and recovers catalytic activity in the cytosol. The pathway that leads to toxicity is also Hrd1p-dependent but, unlike that for the related ricin A chain toxin, SLTxA1 dislocation does require the catalytic cysteine of Hrd1p. However it does not depend on canonical ubiquitylation since toxin variants lacking endogenous lysyl residues also utilize this pathway, and furthermore there is no requirement for a number of Cdc48p co-factors. Conclusions and Significance The fraction of SLTxA1 that disengages from the ERAD pathway thus does so upstream of Cdc48p interactions and downstream of Hrd1p interactions, in a step that possibly involves de-ubiquitylation. Mechanistically therefore, the dislocation of this toxin is quite distinct from that of conventional ERAD substrates that are normally degraded, and the toxins partially characterised to date that do not require the catalytic cysteine of the major Hrd1p component of the dislocation apparatus.
Expression of Myosin Light Chain Kinase in Kidney of Streptozotocin-Induced Diabetic Rats
Huaqing Zhu,Xiaolin Zhang,Li Zuo,Qing Zhou,Shuyu Gui,Wei Wei,Yuan Wang
International Journal of Molecular Sciences , 2006, DOI: 10.3390/i7110510
Abstract: Nephropathy is one of the most common complications of diabetes mellituswhich remains incompletely understood. We reported the expression of myosin light chainkinase (MLCK) in the kidney of diabetic rats and investigated the correlation betweenMLCK and diabetic nephropathy by observing the expression of MLCK. The diabetic modelrats were induced by an intraperitoneal injection of streptozotocin (STZ) and the insulin-treated rats were subcutaneously injected with protamine zine insulin 3u/d. The kidneyswere excised and immersed in 4% polyoxymethylene after 12 weeks later. The expression ofMLCK was analyzed by immunohistochemical staining and Western blot.Immunohistochemical analysis and Western blot assay indicated that the MLCK expressionwas higher in kidney of diabetic rats than that in control and it was decreased in kidney ofinsulin-treated rats. Our results suggested that the over expression of MLCK may be relatedwith the development of diabetic nephropathy.
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