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Search Results: 1 - 10 of 2933 matches for " Shudong Zong "
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GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules
Yong Wang, Wei Song, Shuchun Li, Xin Guan, Shiying Miao, Shudong Zong, SS Koide, Linfang Wang
BMC Cell Biology , 2009, DOI: 10.1186/1471-2121-10-25
Abstract: Stable mRHBDD1 knockdown GC-1 cells were sensitive to apoptotic stimuli, PS341 and UV irradiation. In vitro, they survived and proliferated normally. However, they lost the ability to survive and differentiate in mouse seminiferous tubules.Our findings suggest that mRHBDD1 may be associated with mammalian spermatogenesis.Spermatogenesis generates functional sperm cells from initially undifferentiated germ cells. This involves the proliferation of spermatogonia, meiosis of spermatocytes and the differentiation of spermatids into spermatozoa. It is a complex developmental program in which myriad events take place to ensure that the germ cells reach their proper stages of development at the appropriate times. Normal spermatogenesis requires a well-regulated balance of several processes, including cell proliferation, differentiation and apoptosis.Apoptosis is a key phenomenon during spermatogenesis. For instance, an early, massive wave of germ cell apoptosis occurs at puberty. This event takes place during postnatal weeks 2 to 4 in mice, with a peak after 3 weeks [1-3]. It is estimated that 75% of spermatogenic cells undergo apoptosis during development [4,5], ensuring the maintenance of a critical ratio between maturing germ cells and Sertoli cells [2,6]. Sporadic apoptosis also occurs, primarily in spermatogonia and spermatocytes [2], eliminating defective germ cells with mutated DNA [7].The Rhomboid family comprises polytopic membrane proteins, which may be the most widely-conserved membrane proteins identified to date [8]. They share conserved biochemical properties in all biological kingdoms. Rhomboid proteases, which have been well studied in Drosophila, appear to regulate EGF receptor signalling pathways, thereby controlling growth and development [9,10]. In addition, some yeast Rhomboid proteases have been found to play important roles in mitochondrial membrane remodelling [11], while some parasite proteases containing a Rhomboid domain are important for invasiv
Construction of Social Capital for Orderly Flow of Rural Migrant Workers
Shudong Zhang
Asian Social Science , 2011, DOI: 10.5539/ass.v7n7p204
Abstract: As a result of the changes in the social structure and the system in China, rural migrant workers are active on the economic stage of China's reform and opening up as a particular group, and their flow process and the problem of flow order has aroused extensive attention of scholars. At present, the flow of rural migrant workers is still at a disorderly state, which has brought about a series of social problems. This article attempts to start out from the composition and characteristics of the social capital of rural migrant workers, go into deep analysis of the status quo of disorderly flow of rural migrant workers and further finds out approaches to construction of the social capital of rural migrant workers to ensure orderly flow of rural migrant workers.
A Generic Service Architecture for Secure Ubiquitous Computing Systems  [PDF]
Shudong Chen, Johan Lukkien, Richard Verhoeven
Int'l J. of Communications, Network and System Sciences (IJCNS) , 2012, DOI: 10.4236/ijcns.2012.51007
Abstract: The development of ubiquitous computing systems benefits tremendously from the service-oriented computing concept in seamless interoperation of heterogeneous devices. However, architectures, services interfaces and network implementation of the existing service-oriented systems differ case by case. Furthermore, many systems lack the capability of being applied to resource constrained devices, for example, sensors. Therefore, we propose a standardized approach to present a service to the network and to access a networked service, which can be adopted by arbitrary types of devices. In this approach, services are specified and exposed through a set of standardized interfaces. Moreover, a virtual community concept is introduced to determine a secure boundary within which services can be freely discovered, accessed and composed into applications; a hierarchical management scheme is presented which enables the third party management of services and their underlying resources. In this way, application control logic goes into the network and environment context is dealt with intelligently by the system. A prototype system is developed to validate our ideas. Results show the feasibility of this open distributed system software architecture.
Thought on Improving Civic Awareness of Farmers
Chunyan Chen,Shudong Zhang
Asian Social Science , 2010, DOI: 10.5539/ass.v6n9p123
Abstract: For the time being, great gap still exists between the status quo of civic awareness of farmers and the requirement of construction of a new countryside in China. Thus, exploration into the path of fostering the civic awareness of farmers is an urgent task to construct the new socialist countryside.
A Novel Analytical Method for Structural Characteristics of Gene Networks and its Application  [PDF]
Shudong Wang, Yuanyuan Zhang, Kaikai Li, Dazhi Meng
Computational Molecular Bioscience (CMB) , 2012, DOI: 10.4236/cmb.2012.23009
Abstract: Analyzing gene network structure is an important way to discover and understand some unknown relevant functions and regulatory mechanisms of organism at the molecular level. In this work, mutual information networks and Boolean logic networks are constructed using the methods of reverse modeling based on gene expression profiles in lung tissues with and without cancer. The comparison of these network structures shows that average degree, the proportion of non-isolated nodes, average betweenness and average coreness can distinguish the networks corresponding to the lung tissues with and without cancer. According to the difference of degree, betweenness and coreness of each gene in these networks, nine structural key genes are obtained. Seven of them which are related to lung cancer are supported by literatures. The remaining two genes AKT1 and RBL may have important roles in the formation, development and metastasis of lung cancer. Furthermore, the contrast of these logic networks suggests that the distributions of logic types are obviously different. The structural differences can help us to understand the mechanism of formation and development of lung cancer.
Quantitative Mass spectrometric Analysis of Ropivacaine and Bupivacaine in Authentic, Pharmaceutical and Spiked Human Plasma without Chromatographic Separation
Nahla N. Salama and Shudong Wang
Analytical Chemistry Insights , 2012,
Abstract: The present study employs time of flight mass spectrometry for quantitative analysis of the local anesthetic drugs ropivacaine and bupivacaine in authentic, pharmaceutical and spiked human plasma as well as in the presence of their impurities 2,6-dimethylaniline and alkaline degradation product. The method is based on time of flight electron spray ionization mass spectrometry technique without preliminary chromatographic separation and makes use of bupivacaine as internal standard for ropivacaine, which is used as internal standard for bupivacaine. A linear relationship between drug concentrations and the peak intensity ratio of ions of the analyzed substances is established. The method is linear from 23.8 to 2380.0 ng mL-1 for both drugs. The correlation coefficient was ≥0.996 in authentic and spiked human plasma. The average percentage recoveries in the ranges of 95.39%–102.75% was obtained. The method is accurate (% RE < 5%) and reproducible with intra- and inter-assay precision (RSD% < 8.0%). The quantification limit is 23.8 ng mL-1 for both drugs. The method is not only highly sensitive and selective, but also simple and effective for determination or identification of both drugs in authentic and biological fluids. The method can be applied in purity testing, quality control and stability monitoring for the studied drugs.
Quantitative Mass spectrometric Analysis of Ropivacaine and Bupivacaine in Authentic, Pharmaceutical and Spiked Human Plasma without Chromatographic Separation
Nahla N. Salama,Shudong Wang
Analytical Chemistry Insights , 2009,
Abstract: The present study employs time of flight mass spectrometry for quantitative analysis of the local anesthetic drugs ropivacaine and bupivacaine in authentic, pharmaceutical and spiked human plasma as well as in the presence of their impurities 2,6-dimethylaniline and alkaline degradation product. The method is based on time of flight electron spray ionization mass spectrometry technique without preliminary chromatographic separation and makes use of bupivacaine as internal standard for ropivacaine, which is used as internal standard for bupivacaine. A linear relationship between drug concentrations and the peak intensity ratio of ions of the analyzed substances is established. The method is linear from 23.8 to 2380.0 ng mL-1 for both drugs. The correlation coefficient was ≥0.996 in authentic and spiked human plasma. The average percentage recoveries in the ranges of 95.39%–102.75% was obtained. The method is accurate (% RE < 5%) and reproducible with intra- and inter-assay precision (RSD% < 8.0%). The quantification limit is 23.8 ng mL-1 for both drugs. The method is not only highly sensitive and selective, but also simple and effective for determination or identification of both drugs in authentic and biological fluids. The method can be applied in purity testing, quality control and stability monitoring for the studied drugs.
Pathological and immunohistochemical study of lethal primary brain stem injuries
Sun Rongchao, Yang Shudong, Zhou Zhiyi
Diagnostic Pathology , 2012, DOI: 10.1186/1746-1596-7-54
Abstract: A total of 65 patients who had died of PBSI and other conditions were randomly selected. They were divided into 2 groups, an injury group (25 cases) and a control group (20 cases). Slides of each patient’s midbrain, pons, and medulla oblongata were prepared and stained with HE, argentaffin, and immunohistochemical agents (GFAP, NF, amyloid-?, MBP). Under low power (×100) and NF staining, the diameter of the thickest longitudinal axon was measured at its widest point. Ten such diameters were collected for each part of the brain (midbrain, pons, and medulla oblongata). Data were recorded and analyzed statistically.Brain stem contusions, astrocyte activity, edema, and pathological changes in the neurons were visibly different in the injury and control groups (P?<?0.05). Characteristic changes occurred in the neural axons, axon diameter varied from axon to axon and even over different segments of one axon, and several pathological phenomena were observed. These included segmental thickening and curving, wave-like processing, disarrangement, and irregular swelling. A few axons ruptured and intumesced into retraction balls. Immunohistochemical MBP staining showed enlargement and curving of spaces between the myelin sheaths and axons in certain areas. The myelin sheaths lining the surfaces of the axons were in some cases incomplete and even exfoliated, and segmentation disappeared. These pathological changes increased in severity over time (P?<?0.05).These histopathological changes may prove beneficial to the pathological diagnosis of PBSI during autopsy. The measurement of axon diameters provides a referent quantitative index for the diagnosis of the specific causes of death involved in PBSI.The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1345298818712204Brain stem injury is injury to the midbrain, pons, or medulla oblongata. It is a very serious form of brain injury and can be divided into two types: 1) primary brain
The Structure of Background-error Covariance in a Four-dimensional Variational Data Assimilation System: Single-point Experiment

Juanjuan Liu,Bin Wang,Shudong Wang,

大气科学进展 , 2010,
Abstract: A four dimensional variational data assimilation (4DVar) based on a dimension-reduced projection (DRP-4DVar) has been developed as a hybrid of the 4DVar and Ensemble Kalman filter (EnKF) concepts. Its good flow-dependent features are demonstrated in single-point experiments through comparisons with adjoint-based 4DVar and three-dimensional variational data (3DVar) assimilations using the fifth-generation Pennsylvania State University-National Center for Atmospheric Research Mesoscale Model (MM5). The results reveal that DRP-4DVar can reasonably generate a background error covariance matrix (simply B-matrix) during the assimilation window from an initial estimation using a number of initial condition dependent historical forecast samples. In contrast, flow-dependence in the B-matrix of MM5 4DVar is barely detectable. It is argued that use of diagonal estimation in the B-matrix of the MM5 4DVar method at the initial time leads to this failure. The experiments also show that the increments produced by DRP-4DVar are anisotropic and no longer symmetric with respect to observation location due to the effects of the weather trends captured in its B-matrix. This differs from the MM5 3DVar which does not consider the influence of heterogeneous forcing on the correlation structure of the B-matrix, a condition that is realistic for many situations. Thus, the MM5 3DVar assimilation could only present an isotropic and homogeneous structure in its increments.
Adaptive Hybrid ant colony optimization for solving Dual Resource Constrained Job Shop Scheduling Problem
Jingyao Li,Shudong Sun,Yuan Huang
Journal of Software , 2011, DOI: 10.4304/jsw.6.4.584-594
Abstract: This paper presents a scheduling approach, based on Ant Colony Optimization (ACO), developed to address the scheduling problem in manufacturing systems constrained by both machines and heterogeneous workers called as Dual Resource Constrained Job Shop Scheduling Problem with Heterogeneous Workers. This hybrid algorithm utilizes the combination of ACO and Simulated Annealing (SA) algorithm and proposes an adaptive control mechanism based on ant flow of route choice to improve the global search ability. Two adaptive adjusting schemes of parameters based on iteration times and quality of solutions respectively are imposed to actualize the performance optimization by stages. Then the performances of
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