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Search Results: 1 - 10 of 43009 matches for " Sheng-Lon Lee "
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Effect of Pre-Ageing on the Precipitation Behaviors and Mechanical Properties of Al-7Si-Mg Alloys  [PDF]
Wen-Chi Chen, Sheng-Lon Lee, An-Hung Tan
Journal of Materials Science and Chemical Engineering (MSCE) , 2018, DOI: 10.4236/msce.2018.63005
Abstract: For alloys that are quenched to 25℃ room temperature, there are Mg-Mg, Si-Si and Mg-Si clusters shown by exothermic peaks in a A357 alloy. However, there are no clear Mg-Mg, Si-Si and Mg-Si cluster exothermic peaks in a A356 alloy. Hence, the low Mg content in a A356 alloy has less impact with natural ageing. The natural ageing impact on the mechanical properties of a A357 alloy is higher than a A356 alloy due to the precipitation of Mg-Si clusters for which the nucleation size does not reach the critical size. The 90℃ pre-ageing process could promote Mg-Si clusters to a critical size to become the nucleation site for of β''. This then increases the artificial ageing strength and mitigates the impact of natural ageing.
Detection of Sugar-Regulated Gene Expression and Signaling in Suspension-Cultured Rice Cells  [PDF]
Shin-Lon Ho
American Journal of Plant Sciences (AJPS) , 2018, DOI: 10.4236/ajps.2018.96085
Abstract: To better understand the mechanism of sugar signaling in rice cell, the suspension-cultured rice cells were transferred from sucrose-containing (+S) to sucrose-free (-S) of MS culture medium, we found that ribosomal RNAs (rRNAs) were degraded progressively. This suggests that carbon, nitrogen, and phosphate were recycled in this process and the reduction in cellular rRNAs might lead to decreased translation to save energy in response to sugar starvation. Differential screening revealed that two groups of genes, sugar-starvation-repressed (SSR) and sugar-starvation-activated (SSA) genes, were regulated by sugar in an opposing manner. Northern-blot analysis showed that two major hybridization signals of 0.8 and 1.9 kb were induced strongly under sugar starvation. The two populations of genes corresponded with homologs of α-amylases (1.9 kb) and the glycine-rich proteins (GRPs) gene family (0.8 kb), and all were SSA genes. Expression of GRP genes was strongly induced in sugar-starved cells, which suggests that GRPs may help to protect cells against nutritional stress. Treatment of +S and -S cells with the protein kinase (PK) inhibitor staurosporine (St) and the serine/theronine phosphoprotein phosphatases 1 (PP1) and 2A (PP2A) inhibitor okadaic acid (OA) revealed that PP1 and PP2A (PPs) might be involved in increasing SSR gene expression in +S cells, and that activation of the majority of the SSA genes in -S cells might be due to PKs activity. These results suggested that PKs and PPs might be involved in the sugar regulation of SSR and SSA gene expression. An in-gel PK activity assay demonstrated that the activity of two classes of PKs (50 and 66 kDa) may be induced rapidly after transfer of +S cells to -S medium. Following transfer of -S cells to +S medium, a novel class of 38 kDa PK was induced rapidly and showed high activity. The 38 kDa PK might play a role in sugar sensing, and the 50 and 66 kDa PKs might play roles in signal sensing under sugar starvation in rice cells. These results provide valuable information on three classes of protein kinases that might play key roles in sugar sensing and signaling in rice.
The Dose-Dependent Immunoregulatory Effects of the Nitric Oxide Synthase Inhibitor NG-Nitro-L-Arginine Methyl Ester in Rats with Sub-Acute Peritonitis
Chien-Chou Hsiao, Chien-Hsing Lee, Lon-Yen Tsao, Hui-Chen Lo
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0042467
Abstract: Background Chronic inflammation accompanied by arginine deficiency, immune dysfunction, and excess nitric oxide (NO) production is a clinical condition found in patients with peritonitis. A previous study showed that the nonselective NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME) may facilitate the metabolism of the immune nutrient arginine without altering NO homeostasis in rats with sub-acute peritonitis. Here, we investigated the effects of L-NAME on the immunocytic subpopulation distribution and response. Materials and Methods Male Wistar rats with cecal puncture-induced peritonitis were administered parenteral nutrition solutions supplemented with 0 (CPP group), 5 (LNA group), 25 (MNA group) or 50 (HNA group) mg·kg?1·day?1 of L-NAME for 7 days. Parenteral-fed sham-operated rats (TPN group) and orally-fed healthy rats (R group) were included as controls. Results The TPN group had significantly increased spleen weights and levels of plasma nitrite/nitrate (NOx), circulating white blood cells (WBC), and splenocytic T cells, as well as significantly decreased levels of cytotoxic T- and B-leukocytes and B-splenocytes compared to the R group. The CPP group had significantly decreased levels of plasma NOx and concanavalin (Con) A-stimulated interferon (IFN)-γ and interleukin (IL)-2 production by leukocytes and significantly increased production of Con A-stimulated tumor necrosis factor (TNF)-α and lipopolysaccharide (LPS)-stimulated IFN-γ in the leukocytes. In addition, the LNA and MNA groups had significantly decreased spontaneous IL-6 and Con A-stimulated TNF-α and IFN-γ production by the leukocytes while the HNA group had significantly increased LPS-stimulated TNF-α and Con A-stimulated IFN-γ and IL-2 production by the splenocytes compared to the CPP group. Conclusions Low-dose L-NAME infusion may suppress proinflammatory and T-helper-1 (Th1) response in leukocytes, and high-dose infusion may activate the proinflammatory response in splenic macrophages and Th1 response in T-splenocytes in rats with sub-acute peritonitis.
FREQ-Seq: A Rapid, Cost-Effective, Sequencing-Based Method to Determine Allele Frequencies Directly from Mixed Populations
Lon M. Chubiz, Ming-Chun Lee, Nigel F. Delaney, Christopher J. Marx
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0047959
Abstract: Understanding evolutionary dynamics within microbial populations requires the ability to accurately follow allele frequencies through time. Here we present a rapid, cost-effective method (FREQ-Seq) that leverages Illumina next-generation sequencing for localized, quantitative allele frequency detection. Analogous to RNA-Seq, FREQ-Seq relies upon counts from the >105 reads generated per locus per time-point to determine allele frequencies. Loci of interest are directly amplified from a mixed population via two rounds of PCR using inexpensive, user-designed oligonucleotides and a bar-coded bridging primer system that can be regenerated in-house. The resulting bar-coded PCR products contain the adapters needed for Illumina sequencing, eliminating further library preparation. We demonstrate the utility of FREQ-Seq by determining the order and dynamics of beneficial alleles that arose as a microbial population, founded with an engineered strain of Methylobacterium, evolved to grow on methanol. Quantifying allele frequencies with minimal bias down to 1% abundance allowed effective analysis of SNPs, small in-dels and insertions of transposable elements. Our data reveal large-scale clonal interference during the early stages of adaptation and illustrate the utility of FREQ-Seq as a cost-effective tool for tracking allele frequencies in populations.
Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System
Da-Sheng Lee
Sensors , 2010, DOI: 10.3390/s100100697
Abstract: Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction (PCR) machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design.
A Study of the Form of Organizations: Toward an Integrative Framework of Population Ecology and Institutionalism
Sheng-Hsien Lee
International Journal of Business and Management , 2012, DOI: 10.5539/ijbm.v7n10p108
Abstract: Does the form of organizations tend to show diversity or homogeneity? This paper begins with two contrasting questions: ‘Why are there many organizational forms?’ and ‘Why are organizations similar?’ Population ecology and institutional theory represent different lenses through which theorists use for interpreting these organizational phenomena. Each paradigm offers compelling concepts and arguments for supporting their own perspectives even though they seem to lead to contradictory results. This conceptual paper is an attempt to clarify the fundamental divergence of ecological and institutional approaches and proposes an integrated perspective for future empirical studies.
-directed inverse systems of continuous images of arcs
Ivan Lon ar
International Journal of Mathematics and Mathematical Sciences , 2000, DOI: 10.1155/s016117120000363x
Abstract: The main purpose of this paper is to prove that if X={Xa,pab,A} is a usual ℵ1-directed inverse system of continuous images of arcs with monotone bonding mappings, then X=limX is a continuous image of an arc (Theorem 2.4). Some applications of this statement are also given.
Replication Publication
Mark Patterson,Lon Cardon
PLOS Biology , 2012, DOI: 10.1371/journal.pbio.0030327
Postmodern organization and new forms of organizational control
Lon?ar Dragan
Economic Annals , 2005, DOI: 10.2298/eka0565105l
Abstract: This article displays post bureaucratic organisational concept as an adequate representative of all emerging organizational forms which are natural result of persistent initiatives to flexibly and intensify working process. Under this term we assume all budding ‘sub-representatives' such as Total Quality Management (TQM), Just-in-time concept (JIT), network systems and joint ventures, virtual organizations, teamwork and other related structures. The author concludes that main virtues of new organizational paradigm are flexibility, decentralization, higher employee empowerment, knowledge and information sharing, responsibility for the system as a whole and permanent learning. On the other hand, some downsides become obvious. Those are danger from anarchy, responsibility and stress, greater employees' insecurity and resistance to new practices. Furthermore, the paper shed light on power and identity dynamics through the lens of improved and still intentional methods of organizational control. The main argument is that compulsive desire to control never fades away, only the methods of control takes different, more advanced forms through organizational culture, vocabulary and discourses monitoring at a distance, peer evaluation inside teams, employee selection and many others.
The project "The written-offs" and the deacquisition of library materials at the University Library of Maribor
Nina Lon?ar
Knji?nica : Revija za Podro?je Bibliotekarstva in Informacijske Znanosti , 2010,
Abstract: The process of de-acquisition and weeding is based on the same selection criteria as acquisition of library materials and requires profound knowledge and understanding of a library collection. The author presents the methodology and the process of weeding and de-acquisition at the University Library of Maribor. The case study is the project "The Written-Offs". The lights and art works made out of damaged books exhibited in this project attracted a lot of media and public attention. The results and experiences drawn from the project "The Written-Offs" show a possible way to promote libraries.
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