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Search Results: 1 - 10 of 31687 matches for " Sheila Aparecida Coelho;Pereira "
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Compara??o de três protocolos distintos para extra??o de RNA de amostras fixadas em formalina e emblocadas em parafina
Gouveia, Gisele Rodrigues;Ferreira, Suzete Cleusa;Sabino, Ester Cerdeira;Siqueira, Sheila Aparecida Coelho;Pereira, Juliana;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2011, DOI: 10.1590/S1676-24442011000600012
Abstract: introduction: formalin fixed paraffin embedded (ffpe) tissues are an important sample source for retrospective studies. despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests once it fragments and chemically modifies nucleic acids, particularly rna. objective: to compare the efficiency of three different rna extraction protocols for gene expression analysis of ffep tissues. material and methods: rna was extracted from ffpe samples of human lymph by means of ambion and arcturus bioscience kits and the classical trizol method. after extraction, rna was quantified and tested for amplification through real time polymerase chain reaction (rt-pcr) using glyceraldehydes-3 phosphate dehydrogenase (gapdh) endogenous gene primers. discussion/conclusion: all the protocols produced sufficient and adequate amounts of total rna. however, only protocols using arcturus and ambion kits generated suitable rna for pcr amplification.
Disease progression after R-CHOP treatment associated with the loss of CD20 antigen expression
Bellesso, Marcelo;Xavier, Flavia Dias;Costa, Renata Oliveira;Pereira, Juliana;Siqueira, Sheila Aparecida Coelho;Chamone, Dalton Alencar Fischer;
Revista Brasileira de Hematologia e Hemoterapia , 2011, DOI: 10.5581/1516-8484.20110036
Abstract: a case of a follicular lymphoma transformed into a cd20+ is described which progressed with the loss of cd20 expression after 8 cycles of r-chop. this phenomenon is not a rare event and has shown poor prognosis. our purposes are to describe this event and suggest biopsy in relapsed or progressive disease.
Bcl-2 protein frequency in patients with high-risk diffuse large B-cell lymphoma
Hallack Neto, Abrah?o Elias;Siqueira, Sheila Aparecida Coelho;Dulley, Frederico Luiz;Chauobah, Alfredo;Belesso, Marcelo;Saboia, Rosaura;Ruiz, Milton Artur;Chamone, Dalton Alencar Fischer;Pereira, Juliana;
Sao Paulo Medical Journal , 2010, DOI: 10.1590/S1516-31802010000100004
Abstract: context and objective: gene expression and immunohistochemical profiling of diffuse large b-cell lymphoma (dlbcl) have revealed important prognostic subgroups: germinal center b-cell-like (gcb-like) dlbcl and activated b cell-like (abc-like) dlbcl. although few reports on high-risk dlbcl are available, the prognosis for the gcb-like subgroup has been shown to be better than that of the abc-like subgroup. the role of bcl-2 as a predictor of survival in dlbcl cases is unclear and its expression varies between the two subgroups of dlbcl. in this study, we analyzed the frequency and prognostic impact of bcl-2 protein expression in high-risk dlbcl cases. design and setting: retrospective cohort study among dlbcl patients treated at hospital das clínicas, faculdade de medicina da universidade de s?o paulo (hc-fmusp). methods: the prognostic impact of the expression of the proteins cd10, bcl-6, mum1 (multiple myeloma oncogene-1) and bcl-2 on high-risk dlbcl cases was evaluated by means of immunohistochemistry. seventy-three patients aged 18-60 years were evaluated for all these markers. results: twenty-four cases (32.9%) were gcb-like and 49 (67.1%) were abc-like, with no difference regarding complete remission, disease-free survival or overall survival rates. twenty-seven patients (37%) showed bcl-2 expression, which was the only independent factor predicting a worse prognosis for overall survival according to multivariate analysis. conclusion: bcl-2 protein was expressed in 37% of the high-risk dlbcl patients, without any difference between the abc-like dlbcl and gcb-like dlbcl cases.
The impact of clinical and genetic screenings on the management of the multiple endocrine neoplasia type 1
Louren?o-Jr, Delmar Muniz;Toledo, Rodrigo Almeida;Coutinho, Flavia Lima;Margarido, Leontina Concei??o;Siqueira, Sheila Aparecida Coelho;Santos, Marcelo Augusto Cortina Gon?alves dos;Montenegro, Fabio Luiz de Menezes;Machado, Marcel Cerqueira Cesar;Toledo, Sergio Pereira Almeida;
Clinics , 2007, DOI: 10.1590/S1807-59322007000400014
Abstract: purpose: to perform clinical and genetic screening for multiple endocrine neoplasia type 1 (men1) in patients at the academic hospital of the university of s?o paulo school of medicine, and to analyze its impact on clinical management of patients with men1. methods: the clinical diagnosis of men1 was made in accordance with the consensus on multiple endocrine neoplasias. mutation analysis of the entire men1 tumor suppressor gene and genetic screening of at-risk family members were performed by direct sequencing. to analyze the implementation of genetic diagnosis, the studied patients were separated into 3 groups: men1 index cases (group i), clinically diagnosed men1 cases (group ii), and genetically diagnosed men1 cases (group iii). results: in total, 154 individuals were clinically and genetically studied. we identified 12 different men1 mutations. fifty-two men1 cases were identified: 13 in group i, 28 in group ii, and 11 in group iii. the mean age in group iii (27.0 years) was significantly lower than in groups i (39.5 years) and ii (42.4 years; p = 0.03 and p = 0.01, respectively). patients in groups i and ii mostly presented 2 or 3 men1-related tumors, while 81.8% of those in group iii presented 1 or no men1-related tumor. additionally, in group iii, 45.4% of cases were asymptomatic, and no metastasis or death was verified. surveillance for men1 mutations allowed the exclusion of 102 noncarriers, including a case of men1 phenocopy. conclusion: our data supports the benefits of clinical and genetic screening for multiple endocrine neoplasia type 1 in the management of this syndrome.
Classifica??o dos tumores hematopoéticos e linfoides de acordo com a OMS: padroniza??o da nomenclatura em língua portuguesa, 4a edi??o
Zerbini, Maria Cláudia Nogueira;Soares, Fernando Augusto;Morais, José Carlos;Vassallo, José;Velloso, Elvira Deolinda Rodrigues Pereira;Chaufaille, Maria de Lourdes L. F;Chiattone, Carlos Sergio;Aldred, Vera Lucia;Siqueira, Sheila Aparecida Coelho;Alves, Antonio Correia;Castelli, Jussara Bianchi;Oliveira, Claudia Regina Gomes Mendes Cardim de;Menezes, Yara de;Paes, Roberto Pinto;
Jornal Brasileiro de Patologia e Medicina Laboratorial , 2011, DOI: 10.1590/S1676-24442011000600011
Abstract: introduction: the world health organization (who) classification of hematopoietic and lymphoid tissue (4th edition, 2008) tumors constitutes an updated review of the 3rd edition published in 2001. the translation of the nomenclature used to describe the entities should be clear, precise and uniform so that clinicians, pathologists and researchers involved in the onco-hematopathological area may identify them accurately. objective: with this purpose, the authors present an updated proposal and a terminological standardization in portuguese based on who/2008
Conhecimento e Aplica o de Métodos de Ensino para os Jogos Esportivos Coletivos na Forma o Profissional em Educa o Física
Nilton Ferreira Coutinho,Sheila Aparecida Pereira dos Santos Silva
Movimento , 2009,
Abstract: Esa investigación intenta comprender la realidad pedagógica de los profesores que ense an disciplinas de deportes colectivos en las carreras de Educación Física. Esa investigación de naturaleza fenomenológica, tuvo como retos verificar el conocimiento sobre métodos de ense anza e identificar las razones para su utilización o no en los pregrados que preparan maestros de Educación Física para trabajar en escuelas. Fueron entrevistados 17 personas que imparten esas asignaturas en 9 universidades privadas, siendo 8 de la ciudad de S o Paulo y ciudades de la región del Gran S o Paulo y 1 de la región Baixada Santista. Como resultado podemos decir que hay una utilización predominante de la Metodología Tradicional Tecnicista porque es la metodología que hizo parte de su formación profesional, aunque sea perceptible la emergencia de la utilización de otros métodos: Situacional; Crítico-Superador; Serie de Juegos, Deportivos Modificados, Modelo del péndulo (Claude Bayer) y Crítico-Emancipatório, aún poco conocidos por los profesores de las instituciones investigadas.
Pluralidade cultural: Os esportes radicais na Educa o Física escolar
Igor Armbrust,Sheila Aparecida Pereira dos Santos Silva
Movimento , 2012,
Abstract: El plan de estudios de Educación Física ha sido renovado y se observa que su contenido tradicional, comienzan a compartir espacio en la escuela, devido a su actual divulgación de nuevos contenidos. Los Deportes Radicales se encuentran en esta situación, han atraído la atención de ni os que frecuentan la escuela y han mostrado su potencial educativo de convergencia con los objetivos de la Educación Física. Por el contrario, resistencias se identifican por parte de los educadores, que expresan su preocupación com base en el image de riesgo e imprevisibilidad relacionados a estos contenidos. Ante esta situación, con el apoyo en el pensamiento complejo basado en Edgar Morin, el presente ensayo tiene como objetivo presentar una reflexión sobre la inclusión de los deportes radicales en las propuestas pedagógicas.
Fatal outcome in bronchus-associated lymphoid tissue lymphoma
Mattedi, Romulo Loss;Bernardi, Fabiola del Carlo;Bacchi, Carlos Eduardo;Siqueira, Sheila Aparecida Coelho;Mauad, Thais;
Jornal Brasileiro de Pneumologia , 2007, DOI: 10.1590/S1806-37132007000400021
Abstract: primary pulmonary lymphoma is rare. the most common histological type is the bronchus-associated lymphoid tissue lymphoma. this type of lymphoma has an indolent course and excellent response to therapy. one-third of all cases are diagnosed incidentally. however, due to the rarity of this disease, little is known about its natural history in terms of dissemination and evolution. herein, we report the unusual case of a 61-year-old man who refused treatment after being diagnosed with bronchus-associated lymphoid tissue lymphoma and died 2 years later from massive lung infiltration without dissemination to other organs.
Disseminated neuroendocrine neoplasm with undetected primary tumor
Frederico Becker Ribeiro,Sheila Aparecida Coelho Siqueira,Marianne Castro Gon?alves,Anderson da Costa Lino Costa
Autopsy and Case Reports , 2012,
Abstract: Since the 19th century, neuroendocrine neoplasms (NEN) have been identified. Right up to the present day, the nomenclature is still under debate reflecting the heterogeneity of these tumors. Although some of them are slow growing, some can be fearfully aggressive and may develop in almost any organ of the abdomen, thorax, neck, skin, and gonads. The most commonly observed sites of NEN are the lung and the gastroenteropancreatic system (GEP), where more than 50 entities have been observed. In case of a NEN of unknown primary tumor, the histopathological diagnostic workup includes immunohistochemistry for chromogranin A and synaptophysin, followed by specific tissue markers. Clinical presentation is very diverse, depending on the primary site and functionality of the tumor. In the case of the GEP-NEN, the main symptoms are abdominal pain, diarrhea, weight loss, gastrointestinal bleeding, or bowel obstruction. The presence of neuropsychiatric symptoms is not insignificant in this group of tumors. The authors report a case of a 51-year-old man who sought medical attention because of a three-month history of a consumptive disease. The diagnostic workup disclosed a diffuse nodular infiltration of the lungs, hypokalemia, and hypercalcemia in a cachectic patient. The clinical investigation could not proceed because of an infectious intercurrence, which led to the fatal outcome. Autopsy findings showed a diffuse metastatic NEN. The primary tumoral site could not be demonstrated with the available immunohistochemical panel.
Rosai - Dorfman disease: a rare entity diagnosed at autopsy
Jo?o da Costa Veloso Neto,Sheila Aparecida Coelho Siqueira,Maria Claudia Nogueira Zerbini
Autopsy and Case Reports , 2013,
Abstract: Rosai-Dorfman disease (RDD) or Sinus histiocytosis with massive lymphadenopathy is a rare and benign histiocytic proliferative disorder first described by Juan Rosai and Ronald Dorfman in 1969, whose etiology remains unknown. Since then, many cases were reported in the literature. The disease primarily involves the lymph nodes, and is characterized by painless, bilateral cervical lymphadenopathy accompanied by fever, night sweats, malaise and weight loss, reason why many patients are clinically misdiagnosed as malignant lymphoma. In some cases, extranodal involvement may be present. Leukocytosis, elevated erythrocyte sedimentation rate, and hypergamaglobulinemia are often present. The authors report a case of a 52-year-old female patient admitted to the hospital with the diagnosis of pneumonia and progressed to multiple organs failure and death. During the hospitalization an attempt to diagnose a lymphoproliferative disease trough an axillary lymph node biopsy was disappointing. The autopsy was crucial for the diagnosis, illustrating a severe and unusual presentation of Rosai-Dorfman disease.
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