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Search Results: 1 - 10 of 16184 matches for " Shao-Pei Chou "
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Role of chemokine CCL19 in immune cell mediated antitumor effect

GUAN Shao-pei
, LU Ai-guo

- , 2015, DOI: 11.3969/j.issn.1674-8115.2015.03.030
Abstract: 趋化因子是一类能趋化免疫细胞定向移动的小分子分泌蛋白。趋化因子CCL19和其受体CCR7在树突状细胞、T细胞和多种肿瘤细胞上表达。近年来,CCL19及CCR7在抗肿瘤治疗中的作用成为研究热点并取得显著进展,它们在趋化树突状细胞、CD4+和CD8+ T细胞浸润肿瘤,介导免疫细胞释放细胞因子,抑制肿瘤增殖、迁移和侵袭以及协助治疗肿瘤过程中发挥关键作用,对其深入研究有助于找到新的肿瘤治疗手段和基因疫苗。该文对CCL19在介导免疫细胞抗肿瘤中的作用作一综述。
: Chemokines are a family of small cytokines secreted by immune cells, which act as a chemoattractant to guide the migration of immune cells. It has been reported that CCL19 (chemokine C-C motif ligand 19) and its receptor CCR7 (a seven transmembrane protein) are expressed in dendritic cells, CD4+ and CD8+T cells and variety of tumor cells. Recently, more attention has been focused on the antitumor effect of CCL19 and its receptor CCR7, which has been well-documented in the literature and was vital to attracting dendritic cells, infiltration of tumors by CD4+ and CD8+T cells, mediating immune cells to release cytokines, inhibiting the proliferation, invasion, and migration of tumors, and assisting the treatment of tumors. Further studies on CCL19 and CCR7 are helpful for developing new treatments of tumors and gene vaccines. This paper reviews the role of CCL19 the immune cell mediated antitumor therapy
Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses
Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou, Ya-Wen Tsai, Te-Chin Chu, Chun-Chieh A Shih, Wen-Hsiung Li, Ming-Che Shih
Biotechnology for Biofuels , 2011, DOI: 10.1186/1754-6834-4-24
Abstract: We have developed an efficient platform that uses a combination of transcriptomic and proteomic approaches to N. patriciarum to accelerate gene identification, enzyme classification and application in rice straw degradation. By conducting complementary studies of transcriptome (Roche 454 GS and Illumina GA IIx) and secretome (ESI-Trap LC-MS/MS), we identified 219 putative GH contigs and classified them into 25 GH families. The secretome analysis identified four major enzymes involved in rice straw degradation: β-glucosidase, endo-1,4-β-xylanase, xylanase B and Cel48A exoglucanase. From the sequences of assembled contigs, we cloned 19 putative cellulase genes, including the GH1, GH3, GH5, GH6, GH9, GH18, GH43 and GH48 gene families, which were highly expressed in N. patriciarum cultures grown on different feedstocks.These GH genes were expressed in Pichia pastoris and/or Saccharomyces cerevisiae for functional characterization. At least five novel cellulases displayed cellulytic activity for glucose production. One β-glucosidase (W5-16143) and one exocellulase (W5-CAT26) showed strong activities and could potentially be developed into commercial enzymes.Cellulosic ethanol produced by microbial fermentation from feedstocks has been proposed to replace fossil fuels in transportation. A key step in cellulosic ethanol production is to break down cellulose into glucose and hemicellulose into xylose, which can subsequently be converted into ethanol by fermentative microbes. Therefore, finding efficient cellulases is important to bioethanol production, as well as for hydrolyzing feedstocks into sugars in general. Neocallimastix species is one of the major anaerobic fungi in the rumen of water buffalo capable of efficiently digesting cellulosic biomass [1-4]. Such anaerobic fungi are potential sources for highly active cellulolytic enzymes that are useful for cellulose hydrolysis [5-7]. Plant cell wall degrading enzymes from rumen fungi such as Neocallimastix patriciarum may
Ru/AC Catalyzed Ozonation of Recalcitrant Organic Compounds

WANG Jian-bing,HOU Shao-pei,ZHOU Yun-rui,ZHU Wan-peng,HE Xu-wen,

环境科学 , 2009,
Abstract: Ozonation and Ru/AC catalyzed ozonation of dimethyl phthalate(DMP),phenols and disinfection by-products precursors were studied.It shows that Ru/AC catalyst can obviously enhance the mineralization of organic compounds.In the degradation of DMP,TOC removal was 28.84% by ozonation alone while it was 66.13% by catalytic ozonation.In the oxidation of 23 kinds of phenols,TOC removals were 9.57%-56.08% by ozonation alone while they were 41.81%-82.32% by catalytic ozonation.Compared to ozonation alone,Ru/AC catal...
Forum: A Short-term Research Visit to the Georg Eckert Institute for International Textbook Research
Pei-I Chou
Journal of Textbook Research , 2011,
Surface α-Enolase Promotes Extracellular Matrix Degradation and Tumor Metastasis and Represents a New Therapeutic Target
Kuan-Chung Hsiao, Neng-Yao Shih, Hsun-Lang Fang, Tze-Sing Huang, Ching-Chuan Kuo, Pei-Yi Chu, Yi-Mei Hung, Shao-Wen Chou, Yi-Yuan Yang, Gee-Chen Chang, Ko-Jiunn Liu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0069354
Abstract: In previous research, we found α-enolase to be inversely correlated with progression-free and overall survival in lung cancer patients and detected α-enolase on the surface of lung cancer cells. Based on these findings, we hypothesized that surface α-enolase has a significant role in cancer metastasis and tested this hypothesis in the current study. We found that α-enolase was co-immunoprecipitated with urokinase-type plasminogen activator, urokinase-type plasminogen activator receptor, and plasminogen in lung cancer cells and interacted with these proteins in a cell-free dot blotting assay, which can be interrupted by α-enolase-specific antibody. α-Enolase in lung cancer cells co-localized with these proteins and was present at the site of pericellular degradation of extracellular matrix components. Treatment with antibody against α-enolase in vitro suppressed cell-associated plasminogen and matrix metalloproteinase activation, collagen and gelatin degradation, and cell invasion. Examination of the effect of treatment with shRNA plasmids revealed that down regulation of α-enolase decreases extracellular matrix degradation by and the invasion capacity of lung cancer cells. Adoptive transfer of α-enolase-specific antibody to mice resulted in accumulation of antibody in subcutaneous tumor and inhibited the formation of tumor metastasis in lung and bone. This study demonstrated that surface α-enolase promotes extracellular matrix degradation and invasion of cancer cells and that targeting surface α-enolase is a promising approach to suppress tumor metastasis.
Cellular responding kinetics based on a model of gene regulatory networks under radiotherapy  [PDF]
Jin-Peng Qi, Yong-Sheng Ding, Shi-Huang Shao, Xian-Hui Zeng, Kuo-Chen Chou
Health (Health) , 2010, DOI: 10.4236/health.2010.22021
Abstract: Radiotherapy can cause DNA damage into cells, triggering the cell cycle arrest and cell apop-tosis through complicated interactions among vital genes and their signal pathways. In order to in-depth study the complicated cellular res- ponses under such a circumstance, a novel mo- del for P53 stress response networks is pro- posed. It can be successfully used to simulate the dynamic processes of DNA damage trans-ferring, ATM and ARF activation, regulations of P53-MDM2 feedback loop, as well as the toxins degradation. Particularly, it has become feasible to predict the outcomes of cellular response in fighting against genome stresses. Consequently, the new model has provided a reasonable framework for analyzing the complicated regu-lations of P53 stress response networks, as well as investigating the mechanisms of the cellular self-defense under radiotherapy.
Electrochemical Detection of Zeranol and Zearalenone Metabolic Analogs in Meats and Grains by Screen-Plated Carbon-Plated Disposable Electrodes  [PDF]
Ming-Kun Hsieh, Huiru Chen, Jen-Lin Chang, Wei-Shao She, Chi-Chung Chou
Food and Nutrition Sciences (FNS) , 2013, DOI: 10.4236/fns.2013.47A005

Zeranol (Z) is an estrogenic growth-promoting agent synthesized from mycotoxin zearalenone (Zen). Inadvertent consumption of Z and its structural analogs from meat or grain products remain a food safety concern. An economic and rapid high performance liquid chromatography method with electrochemical detection using disposable screen-printed carbon electrode is developed for determination of Z, Zen and 3 major metabolic analogs α-zearalenol (α-Ze), β-zearalenol (β-Ze), and β-zearalanol (β-Za). The electrochemical method was validated for application in food matrices including beef, pork, feed and cereal after optimized liquid and/or solid-phase extraction procedures. All 5 Z analogs were separated in 10 minutes with the limits of detection ranging from 15 ng/ml for α-Ze and 25 ng/ml for Z and Zen; the limit of quantitation ranged from 40 50 ng/ml. The recoveries were all above 75% regardless of matrix types and extraction procedures. The intra and inter day variations were both less than 6% at the nominal concentration of 1 μg/ml and less than 13% at 100 ng/ml level. Chromatographically time-matched peaks of Z, α-Ze and β-Za were observed in moldy feed, cereal and rice with high productivity, indicating possible grain-specific Zs exposure for animals and human. Proper exercise of preservative procedures for grain and grain products to prevent it from mold production is imperative. The simplicity and reproducibility of this method affords quick and reliable quantitation of multiple types of Z analogs in food products and can offer semi-confirmative information comparable to UV detection and supplementary to ELISA screening.



物理学报 , 1957,
Abstract: This is a critical exposition and analysis of the modern developments in the theory of turbulent motion of an incompressible fluid. We begin with the review of the mixture length theories based upon Reynolds' equations of mean motion. Secondly, we analyze the principal contributions to the theory of homogeneous isotropic turbulence. Thirdly, we discuss the treatment of the general turbulent shear flow by means of Reynolds' equations of mean motion and the dynamical equations of velocity correlations which are derived from the equations of turbulent velocity fluctuation. We also point out at the same time that although this method yields theoretical results which are in better agreement with experiment than the results of the mixture length theories and furthermore the theory also leads to the theoretical distributions ot the mean squares of velocity fluctuation, on account of the presence of the higher order velocity correlations in the equations, it continuously leads to unclosed systems of differential equations and hence meets difficulties which are difficult to overcome. Therefore, based upon the above retrospect of the developments of the theory of turbulence and the recent work on the vorticity structure of the homogeneous isotropic turbulence in its final period of decay, we finally propose a new approach to the turbulence problem: The basic component motion of turbulence is vortex motion due to the action of viscosity of the fluid. The dynamical equations which govern the vortex motion of turbulence are Reynolds' equations of mean motion and the equations of velocity fluctuation derived from the Navier-Stokes equations by the averaging process. We also emphasize the importance of Reynolds' recognition that the turbulent motion of a fluid can be separated into the mean motion and fluctuation. The future theoretical investigation is to look for the vortex motions which are solutions of these two sets of equations. In order to make the solutions of the problem unique and comparable with experimental measurements, they should also satisfy statistical conditions on the distribution of vortices analogous to Kol-mogoroffs condition in his statistical theory of locally isotropic turbulence at high Reynolds number turbulent flows.
Risk Factors of Adolescent Obesity in Taiwan and Its Association with Physical activity, Blood Pressure and Waist Circumference
Yi-Chun Chou,Jen-Sheng Pei
Asian journal of Sports Medicine , 2010,
Abstract: Purpose:This study was conducted to investigate risk factors of obesity and its associated health problems in adolescence in Taiwan.Methods:We enrolled 559 adolescent volunteers with equal numbers of females and males in 2008. Participants were divided into two groups: obese (body mass index [BMI] ?95th percentile) and normal-weight adolescents (BMI: 15th ~ 85th percentile). Each of them completed a questionnaire about lifestyle and parents' stature.Results:Obese mother had high likelihood of having obese girls and boys (P=0.001). Obese girls spent more time in watching TV (P=0.03) and had a higher frequency of having sweetened soft drinks (P=0.016) than normal-weight group. Spending more time in watching TV by girls was associated with a higher frequency of having sweetened soft drinks (P=0.012). In boys, it was associated with higher frequency of eating snack (P=0.018) and larger waist circumference (P=0.011). Boys who had more frequent effective exercise had lower frequency of eating outside (P=0.01) and more services of vegetables (P=0.002). There was no relation between hypertension and adolescent physical activity. Regardless of gender, obese group had higher waist circumference and blood pressure than normal-weight group (p < 0.001).Conclusion:There was a strong association between obesity of mother and adolescent obesity. More physical activity in adolescents is associated with healthier dietary behaviors. Obese adolescents have higher waist circumference and blood pressure.
Comprehensive Evaluation on MIS Using Analytical Hierarchy Process

SHAO Pei-ji,

系统工程理论与实践 , 2000,
Abstract: Based on the system analysis and research,this paper establishes the three classification index systems for the comprehensive evaluation on management information systems,the system construction,system function and application evaluation.With the help of Analytical Hierarchy Process,an AHP structure model was built,and an application example is given.
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