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Search Results: 1 - 10 of 8426 matches for " Seung-Hak Cho2 "
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Identification and extensive analysis of inverted-duplicated HBV integration in a human hepatocellular carcinoma cell line
Jeong Bok1,#, Kwang Joong Kim1,#, Mi-Hyun Park1, Seung-Hak Cho2, Hye-Ja Lee1, Eun-Ju Lee1, Chan Park1 & Jong-Young Lee1,*
BMB Reports , 2012,
Abstract: Hepatitis B virus (HBV) DNA is often integrated intohepatocellular carcinoma (HCC). Although the relationshipbetween HBV integration and HCC development has beenwidely studied, the role of HBV integration in HCCdevelopment is still not completely understood. In the presentstudy, we constructed a pooled BAC library of 9 establishedcell lines derived from HCC patients with HBV infections. Byamplifying viral genes and superpooling of BAC clones, weidentified 2 clones harboring integrated HBV DNA. Screeningof host-virus junctions by repeated sequencing revealed anHBV DNA integration site on chromosome 11q13 in theSNU-886 cell line. The structure and rearrangement ofintegrated HBV DNA were extensively analyzed. An invertedduplicated structure, with fusion of at least 2 HBV DNAmolecules in opposite orientations, was identified in theregion. The gene expression of cancer-related genes increasednear the viral integration site in HCC cell line SNU-886.
Molecular Characterization of Enterotoxigenic Escherichia coli Strains Isolated from Diarrheal Patients in Korea during 2003–2011
Kyung-Hwan Oh, Dong Wook Kim, Su-Mi Jung, Seung-Hak Cho
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096896
Abstract: Enterotoxigenic Escherichia coli (ETEC) is one of the major causes of infectious diarrhea in developing countries. In order to characterize the molecular features of human ETEC isolates from Korea, we investigated the profiles of enterotoxin and colonization factor (CF) genes by polymerase chain reaction (PCR) and performed multilocus sequence typing (MLST) with a total of 291 ETEC strains. The specimens comprised 258 domestic strains isolated from patients who had diarrhea and were from widely separated geographic regions in Korea and 33 inflow strains isolated from travelers visiting other Asian countries. Heat-stable toxin (STh)-possessing ETEC strains were more frequent than heat-labile toxin (LT)-possessing ETEC strains in the domestic isolates, while the detection rates of both enterotoxin genes were similar in the inflow isolates. The profile of CF genes of domestic isolates was similar to that of inflow isolates and the major CF types of the strains were CS3-CS21-CS1/PCF071 and CS2-CS3-CS21. Most of these 2 CF types were detected in ETEC strains that possess both lt and sth genes. The major MLSTST types of domestic isolates were ST171 and ST955. Moreover, the 2 major CF types were usually found concomitantly with the 2 major MLST STs, ST171 and ST955. In conclusion, our genotyping results may provide useful information for guiding the development of geographically specific vaccines against human ETEC isolates.
Antibiotic Resistance of Bacteria Isolated from the Internal Organs of Edible Snow Crabs
Misoon Kim, Tae-Hyung Kwon, Su-Mi Jung, Seung-Hak Cho, Seon Yeong Jin, Nyun-Ho Park, Choong-Gon Kim, Jong-Shik Kim
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070887
Abstract: Antibiotic resistance and microbiota within edible snow crabs are important for the Chionoecetes (snow crab) fishing industry. We investigated these parameters using culture methods and antibiotic susceptibility tests with six internal organs from three species of Chionoecetes. Each sample revealed many unexpected microbial species within Chionoecetes internal organs. On the basis of 16S rRNA sequence analysis of 381 isolates, the most abundant genera identified in Chionoecetes opilio were Acinetobacter spp. (24%), Bacillus spp. (4%), Pseudomonas spp. (34%), Stenotrophomonas spp. (28%), and Agreia spp. (11%). In Chionoecetes sp. crabs, Acinetobacter spp. (23%), Bacillus spp. (12%), and Psychrobacter spp. (20%) were most prevalent, while Agreia spp. (11%), Bacillus spp. (31%), Microbacterium spp. (10%), Rhodococcus spp. (12%), and Agrococcus spp. (6%) were most abundant in C. japonicus. Our antibiotic resistance test found resistance to all nine antibiotics tested in 19, 14, and two of the isolates from C. opilio, Chionoecetes sp., and, C. japonicus respectively. Our results are the first to show that microbes with antibiotic resistance are widely distributed throughout the internal organs of natural snow crabs.
Synergistic inhibition of mesothelioma cell growth by the combination of clofarabine and resveratrol involves Nrf2 downregulation
Yoon-Jin Lee1,2, Jae-Hyuk Im3, David M. Lee4, Ji-Sung Park3, Seong Youn Won2, Moon-Kyun Cho2, Hae-Seon Nam2, Yong-Jin Lee1 & Sang-Han Lee1,3,*
BMB Reports , 2012,
Abstract: We previously reported that MSTO-211H cells have a highercapacity to regulate Nrf2 activation in response to changes inthe cellular redox environment. To further characterize itsbiological significance, the response of Nrf2, a transcriptionfactor that regulates ARE-containing genes, on the synergisticcytotoxic effect of clofarabine and resveratrol was investigatedin mesothelioma cells. The combination treatment showed amarked growth-inhibitory effect, which was accompanied bysuppression of Nrf2 activation and decreased expression ofheme oxygenase-1 (HO-1). While transient overexpression ofNrf2 conferred protection against the cytotoxicity caused bytheir combination, knockdown of Nrf2 expression using siRNAenhanced their cytotoxic effect. Pretreatment with Ly294002, aPI3K inhibitor, augmented the decrease in HO-1 level by theircombination, whereas no obvious changes were observed inNrf2 levels. Altogether, these results suggest that the synergisticcytotoxic effect of clofarabine and resveratrol was mediated, atleast in part, through suppression of Nrf2 signaling.
The Difference in Prognostic Factors between Early Recurrence and Late Recurrence in Estrogen Receptor-Positive Breast Cancer: Nodal Stage Differently Impacts Early and Late Recurrence
Sung Gwe Ahn, Hak Min Lee, Sang-Hoon Cho, Suk Jin Bae, Seung Ah Lee, Seung Hyun Hwang, Joon Jeong, Hy-De Lee
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0063510
Abstract: Background Probability of recurrence in patients with estrogen receptor (ER)-positive breast cancer remains constant for long periods. We compared tumor burden impact on late versus early recurrence in our cohort with long-term follow-up. Methods Five hundred and ninety five patients diagnosed with ER-positive breast cancer between 1989 and 2001 were classified into three groups: early recurrence within 5 years, late recurrence after 5 years, and no recurrence. We identified prognostic factors among the groups using logistic regression analysis. Results At median follow-up of 11.7 years, among 595 ER-positive women, 98 (16.4%) had early recurrence and 58 (9.7%) had late recurrence. On multivariate analysis, higher nodal stage (N0 vs. N2, odds ratio [OR] 3.189; N0 vs. N3, OR 9.948), higher histologic grade (grade 1 vs. grade 2, OR 3.896; grade 1 vs. grade 3, OR 5.945), age >35 years (OR 0.295), and receiving endocrine therapy (OR 0.293) affected early recurrence. Compared to no recurrence, receiving endocrine therapy (OR 0.285) was solely related to decreased risk of late recurrence. Increased risk of early recurrence was noted with the higher nodal stage when early and no recurrences were compared. This phenomenon was not found in late recurrence. In the last comparison between the early and late recurrence, higher nodal stage (N0 vs. N3, OR 16.779) and higher histologic grade (grade 1 vs. grade 3, OR 18.111) repeatedly weighted for early recurrence. Conclusions Nodal burden had an attenuated influence on late recurrence, which suggests that, unlike early recurrence, tumor biology might have a more important role than tumor load for late recurrence in ER-positive disease.
Is Osteosarcoma between the Two Peak Ages Different?  [PDF]
Taehun Kim, Hak Sun Chung, Wan-Hyeong Cho, Dae-Geun Jeon, So Hak Chung
Journal of Cancer Therapy (JCT) , 2018, DOI: 10.4236/jct.2018.91008
Abstract: Background: Unlike two known peak age groups (10 - 14 and older than 65 years of age), there was relatively scant attention in osteosarcoma patients between them, and previous reports had analyzed other than Asians. Therefore we conducted this study with Korean patients and focused on 20 - 40 years of age, then investigated age-specific or race-specific meaningful outcomes. Materials and Methods: 379 patients who were newly diagnosed as osteosarcoma in two Korean major cancer hospitals from September 1986 to July 2015 with more than 2-year follow-up were retrospectively studied. There were 290 patients with 20 - 40 years of age, 60 patients aged 19 or younger, and 29 patients aged 41 or older. Age at diagnosis, gender, location of primary lesion, metastasis at diagnosis, histologic subtypes, histologic response were analyzed as prognostic factors by overall survival (OS) and event-free survival (EFS) rates. Results: Primary pelvic lesion and poor histologic response were significantly unfavorable prognostic factors, but not age-specific. However, proportion of metastasis at diagnosis was significantly highest in 20 - 40 years of age group. For the age at diagnosis, older patients presented poorer 5-year OS and EFS rates, but for 10-year, 20
Inhibition of glutamate dehydrogenase and insulin secretion by KHG26377 does not involve ADP-ribosylation by SIRT4 or deacetylation by SIRT3
Eun-A Kim1,#, Seung-Ju Yang2,#, Soo Young Choi3, Woo Je Lee4,* & Sung-Woo Cho1,*
BMB Reports , 2012,
Abstract: We investigated the mechanisms involved in KHG26377 regulationof glutamate dehydrogenase (GDH) activity, focusing onthe roles of SIRT4 and SIRT3. Intraperitoneal injection of micewith KHG26377 reduced GDH activity with concomitant repressionof glucose-induced insulin secretion. Consistent withtheir known functions, SIRT4 ribosylated GDH and reduced itsactivity, and SIRT3 deacetylated GDH, increasing its activity.However, KHG26377 did not affect SIRT4-mediated ADP-ribosylation/inhibition or SIRT3-mediated deacetylation/activationof GDH. KHG26377 had no effect on SIRT4 protein levels,and did not alter total GDH, acetylated GDH, or SIRT3 proteinlevels in pancreatic mitochondrial lysates. These results suggestthat the mechanism by which KHG26377 inhibits GDHactivity and insulin secretion does not involve ADP-ribosylationof GDH by SIRT4 or deacetylation of GDH by SIRT3.
Retiform Sertoli-Leydig Cell Tumor of the Ovary  [PDF]
Gyu-Bong Yu, Seung-Kyu Choi, Yun-Dan Kang, Choong-Hak Park
International Journal of Clinical Medicine (IJCM) , 2016, DOI: 10.4236/ijcm.2016.79065
Abstract: Sertoli-Leydig cell tumor of the ovary is a kind of sex cord-stromal tumor, which occurs between teens and twenties with symptoms including abdominal pain and swelling. The incidence rate is infinitely rare comprising less than 0.5% of all ovarian tumor. The average age of “retiform Sertoli-Leydig cell tumor” is 17 years as compared to 25 years for Sertoli-Leydig cell tumors as a group. We have experienced this rare case of retiform Sertoli-Leydig cell tumor in a 25-year-old foreign patient with the complaint of palpable mass on the right lower quadrant and an irregular menstrual period. The patient underwent right salpingo-oophorectomy and tumor stage was FIGO stage 1A. We report with a brief review of literature.
Endogenous Endophthalmitis Associated with Facial Cellulitis After a Tongue Bite  [PDF]
Min Ho Kang, Mincheol Seong, Ju Hak Lee, Hee Yoon Cho
Open Journal of Ophthalmology (OJOph) , 2012, DOI: 10.4236/ojoph.2012.23018
Abstract: Endogenous endophthalmitis is an intraocular infection that results from hematogenous spread of organisms from a distant source of infection. Facial cellulitis is rarely reported as a focus of infection. We report a case of 51-year-old unconscious woman presenting with fever, facial swelling, and decreased visual acuity secondary to facial cellulitis, endogenous endophthalmitis and end-stage renal disease(ESRD). Generally systemic antibiotics in endophthalmitis have low efficacy because of the blood-ocular barrier. The management of endophthalmitis begins with intravitreal antibiotic injections and if the response is not favorable to do a vitrectomy. Twelve hours after the intravitreal antibiotic injection, vitrectomy was considered because of worsening of the vitreal cloudiness. However, the patient's general medical condition precluded vitrectomy. We experienced successful treatment with intravitreal antibiotic injection and continuous intravenous antibiotic administration because of the breakdown of the blood-ocular barrier due to ocular inflammation, especially in the setting of systemic vascular and fluid homeostatic changes.
Hwanggeumchal sorghum Induces Cell Cycle Arrest, and Suppresses Tumor Growth and Metastasis through Jak2/STAT Pathways in Breast Cancer Xenografts
Jin Hee Park, Pramod Darvin, Eun Joung Lim, Youn Hee Joung, Dae Young Hong, Eui U. Park, Seung Hwa Park, Soo Keun Choi, Eon-Soo Moon, Byung Wook Cho, Kyung Do Park, Hak Kyo Lee, Myong-Jo Kim, Dong-Sik Park, Ill-Min Chung, Young Mok Yang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0040531
Abstract: Background Cancer is one of the highly virulent diseases known to humankind with a high mortality rate. Breast cancer is the most common cancer in women worldwide. Sorghum is a principal cereal food in many parts of the world, and is critical in folk medicine of Asia and Africa. In the present study, we analyzed the effects of HSE in metastatic breast cancer. Methodology/Principal Findings Preliminary studies conducted on MDA-MB 231 and MCF-7 xenograft models showed tumor growth suppression by HSE. Western blotting studies conducted both in vivo and in vitro to check the effect of HSE in Jak/STAT pathways. Anti-metastatic effects of HSE were confirmed using both MDA-MB 231 and MCF-7 metastatic animal models. These studies showed that HSE can modulate Jak/STAT pathways, and it hindered the STAT5b/IGF-1R and STAT3/VEGF pathways not only by down-regulating the expression of these signal molecules and but also by preventing their phosphorylation. The expression of angiogenic factors like VEGF, VEGF-R2 and cell cycle regulators like cyclin D, cyclin E, and pRb were found down-regulated by HSE. In addition, it also targets Brk, p53, and HIF-1α for anti-cancer effects. HSE induced G1 phase arrest and migration inhibition in MDA-MB 231 cells. The metastasis of breast cancer to the lungs also found blocked by HSE in the metastatic animal model. Conclusions/Significance Usage of HS as a dietary supplement is an inexpensive natural cancer therapy, without any side effects. We strongly recommend the use of HS as an edible therapeutic agent as it possesses tumor suppression, migration inhibition, and anti-metastatic effects on breast cancer.
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