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Search Results: 1 - 10 of 21306 matches for " Seong Hwan Kim "
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CK2 Inhibitor CX-4945 Blocks TGF-β1-Induced Epithelial-to-Mesenchymal Transition in A549 Human Lung Adenocarcinoma Cells
Jiyeon Kim, Seong Hwan Kim
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074342
Abstract: Background The epithelial-to-mesenchymal transition (EMT) is a major phenotype of cancer metastasis and invasion. As a druggable cancer target, the inhibition of protein kinase CK2 (formally named to casein kinase 2) has been suggested as a promising therapeutic strategy to treat EMT-controlled cancer metastasis. This study aimed to evaluate the effect of the CK2 inhibitor CX-4945 on the processes of cancer migration and invasion during the EMT in A549 human lung adenocarcinoma cells. Materials and Methods The effect of CX-4945 on TGF-β1-induced EMT was evaluated in A549 cells treated with TGF-β1 (5 ng/ml) and CX-4945. The effect of CX-4945 on TGF-β1-induced cadherin switch and activation of key signaling molecules involved in Smad, non-Smad, Wnt and focal adhesion signaling pathways were investigated by Western blot analysis, immunocytochemistry and reporter assay. Additionally, the effect of CX-4945 on TGF-β1-induced migration and invasion was investigated by wound healing assay, Boyden chamber assay, gelatin zymography, and the quantitative real-time PCR. Results CX-4945 inhibits the TGF-β1-induced cadherin switch and the activation of key signaling molecules involved in Smad (Smad2/3, Twist and Snail), non-Smad (Akt and Erk), Wnt (β-catenin) and focal adhesion signaling pathways (FAK, Src and paxillin) that cooperatively regulate the overall process of EMT. As a result, CX-4945 inhibits the migration and invasion of A549 cells accompanied with the downregulation of MMP-2 and 9. Conclusions Clinical evaluation of CX-4945 in humans as a single agent in solid tumors and multiple myeloma has established its promising pharmacokinetic, pharmacodynamic, and safety profiles. Beyond regression of tumor mass, CX-4945 may be advanced as a new therapy for cancer metastasis and EMT-related disorders.
Effect of Liquid Pig Manure and Chemical Fertilizers on Shoot Growth and Nitrogen Status of Young “Fuyu” Persimmon Trees  [PDF]
Seong-Tae Choi, Gwang-Hwan Ahn, Seong-Cheol Kim, Eun-Seok Kim
Journal of Agricultural Chemistry and Environment (JACEN) , 2017, DOI: 10.4236/jacen.2017.63009
Abstract: Liquid pig manure (LPM), digested from pig slurry, has been used as a nutrient source substituting chemical fertilizer (CF) for some crops. This experiment was conducted to evaluate the effect of different levels of CF and LPM in early July on nitrogen (N) uptake of pot-grown young Fuyu persimmon (Diospyros kaki). The total N and potassium (K) from CF and LPM applied to a 3 L pot were 1.2 g N and 1.15 g K for the low and 2.4 g N and 2.3 g K for the high level. From 2 weeks after the applications, secondary shoots started to grow for the CF but none for the LPM. Two nutrient sources did not significantly affect the amount of N increase in different tree parts from July 1 to August 6. At the high level, tree total N increased by 80% from 551 mg for the CF and by 31% from 583 mg for the LPM. The nutrient sources did not affect soil pH. The soil that received LPM contained more organic matter (P = 0.048), available phosphorus (P) (P = 0.002), and exchangeable K+ (P = 0.001) and Mg2+ (P = 0.009) than the soil that received CF on August 6. These results indicated that N in LPM becomes available later but its effect is more durable than CF.
Nutrient Release during Residue Decomposition of Weeds Mown at Different Times in a Persimmon Orchard  [PDF]
Seong-Tae Choi, Seong-Cheol Kim, Gwang-Hwan Ahn, Doo-Sang Park, Eun-Seok Kim
Journal of Agricultural Chemistry and Environment (JACEN) , 2017, DOI: 10.4236/jacen.2017.64010
Abstract: Decomposition and nutrient release of the residue subsequent to mowing weeds remain poorly understood in persimmon orchards of South Korea. The litterbags including various weed residues were deposited on the soil surface under the tree canopy to simulate the fate of weeds mowed on 13 May, 13 July, and 13 September 2011 and 2012. Rate of decomposition and nutrient release of the residues depended on different mowing times. Residual dry mass (DM) of the 13 May weeds decreased by 17% - 21% of initial DM during 1 month and by 63% -71% until 2 months after litterbag deposition, and they?released 51% - 67% of nitrogen (N), 54% - 55% of phosphorus (P), and 92% - 94% of potassium (K) of respective initial amount until the first 2 months. The 13?July weeds rapidly decomposed during the first month, accounting for 51% - 64% of DM and released 49% - 67% of N, 27% - 54% of P, and 76% - 77% of K. When mowed on 13 September, the weed residue decomposed slower and?longer than the 13 May and 13 July weeds,
Fisetin Inhibits Hyperglycemia-Induced Proinflammatory Cytokine Production by Epigenetic Mechanisms
Hye Joo Kim,Seong Hwan Kim,Jung-Mi Yun
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/639469
Abstract:
Fisetin Inhibits Hyperglycemia-Induced Proinflammatory Cytokine Production by Epigenetic Mechanisms
Hye Joo Kim,Seong Hwan Kim,Jung-Mi Yun
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/639469
Abstract: Diabetes is characterized by a proinflammatory state, and several inflammatory processes have been associated with both type 1 and type 2 diabetes and the resulting complications. High glucose levels induce the release of proinflammatory cytokines. Fisetin, a flavonoid dietary ingredient found in the smoke tree (Cotinus coggygria), and is also widely distributed in fruits and vegetables. Fisetin is known to exert anti-inflammatory effects via inhibition of the NF- B signaling pathway. In this study, we analyzed the effects of fisetin on proinflammatory cytokine secretion and epigenetic regulation, in human monocytes cultured under hyperglycemic conditions. Human monocytic (THP-1) cells were cultured under control (14.5?mmol/L mannitol), normoglycemic (NG, 5.5?mmol/L glucose), or hyperglycemic (HG, 20?mmol/L glucose) conditions, in the absence or presence of fisetin. Fisetin was added (3–10? M) for 48?h. While the HG condition significantly induced histone acetylation, NF- B activation, and proinflammatory cytokine (IL-6 and TNF- ) release from THP-1 cells, fisetin suppressed NF- B activity and cytokine release. Fisetin treatment also significantly reduced CBP/p300 gene expression, as well as the levels of acetylation and HAT activity of the CBP/p300 protein, which is a known NF- B coactivator. These results suggest that fisetin inhibits HG-induced cytokine production in monocytes, through epigenetic changes involving NF- B. We therefore propose that fisetin supplementation be considered for diabetes prevention. 1. Introduction Diabetes and its complications are known for their chronic inflammatory properties. Inflammation is a defense reaction of host tissue against diverse insults. Although normally a beneficial process, when it occurs over prolonged periods, inflammation can be deleterious to cells [1]. Hyperglycemia has been implicated in diabetes-induced inflammatory disease and several diabetes-related complications [2, 3]. It has been reported to induce oxidative stress [4, 5]. In addition, high levels of glucose can activate the proinflammatory transcription factor nuclear κB (NF-κB), resulting in increased inflammatory chemokine and cytokine release [6, 7]. We and other researchers have recently shown that diabetic conditions activate inflammatory gene expression and monocyte activation by inducing epigenetic changes and chromatin remodeling [8, 9]. However, the exact molecular mechanisms induced by hyperglycemia are not fully resolved. NF-κB is required for the expression of many inflammatory genes. Several of these genes have been associated
Cognitive Profiles and Subtypes of Patients with Mild Cognitive Impairment: Data from a Clinical Follow-Up Study  [PDF]
Kyung Won Park, Eun-Joo Kim, Hwan Joo, Sung-Man Jeon, Seong-Ho Choi, Jay C. Kwon, Byoung Gwon Kim, Jae Woo Kim
International Journal of Clinical Medicine (IJCM) , 2012, DOI: 10.4236/ijcm.2012.35068
Abstract: Background: Mild cognitive impairment (MCI) is a heterogeneous condition with a variety of clinical outcomes, the presence of which correlates with risk of Alzheimer’s disease as well as pre-clinical stages of other dementia subtypes. The aims of this study were to assess the specific patterns of cognitive profiles and to identify changes from baseline to 24 weeks in patients with MCI using detailed neuropsychological testing. Methods: We consecutively recruited 120 MCI patients at baseline according to the Petersen’s clinical diagnostic criteria, who were admitted to the Dementia and Memory Clinics. We analyzed patients who fulfilled both inclusion and exclusion criteria for MCI and classified them into four subtypes according to deficits in major cognitive domains; amnestic MCI single domain (aMCI-s), amnestic multiple domain MCI (aMCI-m), non-amnestic single domain MCI (naMCI-s) and non-amnestic multiple domain MCI (naMCI-m). Four groups of MCI were evaluated by a detailed neuropsychological battery test. Results: 83 patients with MCI at the 24-week follow-up were classified into four subtypes. The most frequent subtype was amnestic multi-domain MCI, with the frequency of MCI subtypes as follows: aMCI-s (n = 21, 25.3%), aMCI-m (n = 53, 63.9%), naMCI-s (n = 5, 6.0%) and naMCI-m (n = 4, 4.8%). In the major cognitive items of the SNSB-D, there were significant changes between the initial and follow-up tests in the domains of language, memory and the fron-tal/executive function (p < 0.05), except for attention, in all MCI patient subtypes. At 24-weeks follow-up, the conversion rate to Alzheimer’s disease was 2.4% (n = 2) from a subtype of amnestic multi-domain MCI. Conclusions: Our study revealed the most frequent subtype of MCI to be multiple domain amnestic MCI, with this subtype having a higher tendency of conversion to Alzheimer’s disease.
Variable-Length Feedback Codes under a Strict Delay Constraint
Seong Hwan Kim,Dan Keun Sung,Tho Le-Ngoc
Mathematics , 2015, DOI: 10.1109/LCOMM.2015.2398866
Abstract: We study variable-length feedback (VLF) codes under a strict delay constraint to maximize their average transmission rate (ATR) in a discrete memoryless channel (DMC) while considering periodic decoding attempts. We first derive a lower bound on the maximum achievable ATR, and confirm that the VLF code can outperform non-feedback codes with a larger delay constraint. We show that for a given decoding period, as the strict delay constraint, L, increases, the gap between the ATR of the VLF code and the DMC capacity scales at most on the order of O(L^{-1}) instead of O(L^{-1/2}) for non-feedback codes as shown in Polyanskiy et al. ["Channel coding rate in the finite blocklengh regime," IEEE Trans. Inf. Theory, vol. 56, no. 5, pp. 2307-2359, May 2010.]. We also develop an approximation indicating that, for a given L, the achievable ATR increases as the decoding period decreases.
Real-time PCR Assay Based Glyceraldehydes 3-Phosphate Gene for Identification of Brucella sp.
Seong Guk Kim,Yeong Hwan Kim,Myeong Ju Chae,Jong Wan Kim,Young Ju Lee
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2010.2315.2320
Abstract: Brucellosis is one of the most important zoonoses which affects both animals and humans and leads to serious economic and public health problems. The aim of this study was to design, optimize and evaluate real-time PCR assay for Brucella sp. detection by targeting gap gene and to compare to those of conventional PCR assays. A low variation in CT values was observed for the gap gene target when the same quantity of DNA for 5 Brucella reference strains was used as template in the assays (CT: 21-23 with 500 pg of Brucella DNA). No amplification products were observed in real-time PCR whatever the target with any of the 50 non-Brucella organisms tested. In the analytical sensitivity of real-time PCR assay based gap gene of B. abortus biovar 1 RB51, DNA concentration of 5 fg was successfully amplified and the sensitivity of the gap-based TaqMan real-time PCR assay was identical and 10-100 times higher than the sensitivity of the three conventional PCR. In the clinical trial, 9 (16.3%) and 11 (21.2%) among 52 blood samples from cows confirmed with B. abortus infection by Rose Bengal Spot agglutination test were positive in culture of B. abortus and gap real-time PCR, respectively. In conclusion, the use of the gap-based TaqMan real-time PCR assay appears promising due to it high sensitivity for the simple, faster and specific detection of the Brucella sp.
Optical properties of Er-doped GaN
H. Casta?eda López,Seong-Il Kim,Young-Hwan Kim,Yong Tae Kim
Revista mexicana de física , 2007,
Abstract: Las propiedades ′opticas de epicapas de GaN dopadas con Er (erbium) se han investigado usando fotoluminiscencia (FL). Varias dosis de iones de Er se implantaron en epicapas de GaN por implantaci′on de iones. Se observaron l′ neas claramente visibles de emisi′on verde debidas a la transici′on de la capa interna 4f para Er3+ del espectro de FL de GaN implantado con Er. El espectro de emisi′on consiste en dos l′ neas verdes delgadas a 537 y 558 nm. Las l′ neas de emisi′on verdes se identifican como transiciones del Er3+ de los niveles 5H11/12 y 4S3/2 al estado base 4I15/2; los picos m′as fuertes en la muestra de 5£1014 cm 2, junto con la intensidad relativamente m′as alta de la luminiscencia Er3+ en la muestra dopada inferior. Esto implica que a′un permanece alg′un da no en la muestra de 1£1015 cm 2. Las posiciones pico de las l′ neas de emisi′on debidas a las transiciones internas 4f para Er3+ no cambian con la temperatura creciente. Esto indica que la emisi′on relacionada con Er3+ depende muy poco de la temperatura del ambiente.
Discovery and Development of Anti-HBV Agents and Their Resistance
Kyun-Hwan Kim,Nam Doo Kim,Baik-Lin Seong
Molecules , 2010, DOI: 10.3390/molecules15095878
Abstract: Hepatitis B virus (HBV) infection is a prime cause of liver diseases such as hepatitis, cirrhosis and hepatocellular carcinoma. The current drugs clinically available are nucleot(s)ide analogues that inhibit viral reverse transcriptase activity. Most drugs of this class are reported to have viral resistance with breakthrough. Recent advances in methods for in silico virtual screening of chemical libraries, together with a better understanding of the resistance mechanisms of existing drugs have expedited the discovery and development of novel anti-viral drugs. This review summarizes the current status of knowledge about and viral resistance of HBV drugs, approaches for the development of novel drugs as well as new viral and host targets for future drugs.
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