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Search Results: 1 - 10 of 1085 matches for " Satoru Yanagisawa "
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25-hydroxycholesterol enhances cytokine release and toll-like receptor 3 response in airway epithelial cells
Koarai Akira,Yanagisawa Satoru,Sugiura Hisatoshi,Ichikawa Tomohiro
Respiratory Research , 2012, DOI: 10.1186/1465-9921-13-63
Abstract: Background 25-hydroxycholesterol (25-HC) is one of the oxysterols, which are oxidized derivatives of cholesterol, and has been reported to be involved in the pathogenesis of atherosclerosis and Alzheimer’s disease. In lung, the possible involvement of 25-HC in airway diseases has been revealed. In the present study, we examined whether 25-HC affects the release of cytokines and also modulates the responses of toll-like receptor 3 (TLR3) in airway epithelial cells. Methods The effect of 25-HC on the release of cytokines from primary human bronchial epithelial cells after stimulation with or without polyinosine-polycytidylic acid [poly(I:C)], a ligand for TLR3, and the signal transduction were examined. Results 25-HC significantly potentiated the release of interleukin-8 (IL-8) and IL-6 from the cells. This effect was more potent compared with that of other oxysterols, 22-HC and 27-HC. GW3965 and TO901317, synthetic agonists of liver X receptors that are receptors for oxysterols, did not augment the IL-8 release. 25-HC enhanced the nuclear factor-kappa B (NF-κB) DNA binding activity and translocation of phosphorylated c-Jun into the nucleus. The release of IL-8 was inhibited by the NF-κB inhibitor, caffeic acid phenethyl ester (CAPE), an inhibitor of nuclear factor kappa-B alpha (IκBα) inhibitor, BAY 11–7085, and an inhibitor of nuclear factor kappa-B kinase-2 (IKK-2) inhibitor, SC-514, but not by a c-Jun N-terminal kinase (JNK) inhibitory peptide, L-JNKi1. 25-HC significantly potentiated IL-8 release in poly(I:C)-treated cells and the augmentation was inhibited by CAPE, BAY 11–7085, and SC-514. Furthermore, 25-HC potentiated the translocation of interferon regulatory factor 3 into the nucleus and the release of interferon-beta (IFN-β) in poly(I:C)-treated cells. Conclusions These data demonstrated that 25-HC augments the release of IL-8 and IL-6 via NF-κB signalling pathway and enhances the release of IL-8 and IFN-β after stimulation of TLR3 in airway epithelial cells. 25-HC may be involved in the neutrophilic airway inflammation through the stimulant effect of IL-8 and IL-6 release and also potentiate the TLR3-mediated innate immunity in airway diseases.
The Influence of Free 3-Nitrotyrosine and Saliva on the Quantitative Analysis of Protein-Bound 3-Nitrotyrosine in Sputum
Kazuhito Ueshima,Yoshiaki Minakata,Hisatoshi Sugiura,Satoru Yanagisawa
Analytical Chemistry Insights , 2007,
Abstract: Background: We have recently developed a new technique for quantitatively measuring protein-bound 3-nitrotyrosine (3-NT), a footprint of nitrosative stress, utilizing high-performance liquid chromatography with an electrochemical detection (HPLC-ECD) system. Using this system, we showed that 3-NT formation was upregulated in the sputum of both COPD and asthmatic patients. However, in order to improve the accuracy of the measurement system. We have to resolve some problems which were the influence of free amino acid form of 3-NT and of salivary contamination. Objectives: We initially investigated the amount of the free amino acid form of 3-NT in induced sputum and compared with that of protein-bound 3-NT. Next, we evaluated the concentration of protein-bound 3-NT in saliva and compared with that in induced sputum by means of HPLC-ECD.Methods: Five male COPD patients were enrolled. Induced sputum and saliva were obtained from the patients. The free amino acid form of 3-NT in sputum and saliva was measured by HPLC-ECD, and the protein-bound 3-NT and tyrosine in sputum and saliva were enzymatically hydrolyzed by Streptomyces griseus Pronase and measured for the protein hydrolysate by HPLC-ECD.Results: The mean value of the amount of protein-bound 3-NT was 65.0 fmol (31.2 to 106.4 fmol). On the other hand, the amount of the free amino acid form of 3-NT was under the detection limit (<10 fmol). The levels of both 3-NT (sputum: 0.55 ± 0.15 pmol/ml, saliva: 0.02 ± 0.01 pmol/ml, p < 0.01) and tyrosine (sputum: 0.81 ± 0.43 μmol/ml, saliva: 0.07 ± 0.04 μmol/ml, p < 0.01) in saliva were significantly lower than in sputum. The percentage of 3-NT in saliva to that in sputum was about 3.1%, and that of tyrosine was about 9.0%.Conclusion: The free amino acid form of 3-NT does not affect the measurement of protein-bound 3-NT. Furthermore, the influence of salivary contamination on the measurement of protein-bound 3-NT in induced sputum by means of HPLC-ECDwas very small and could be negligible.
Possibility of sandwiched liver surgery with molecular targeting drugs, cetuximab and bevacizumab on colon cancer liver metastases: a case report
Yoichi Toyama, Takuro Ushigome, Kazuhiro Watanabe, Hiroaki Kitamura, Shinji Onda, Ryota Saito, Seiya Yoshida, Hidejiro Kawahara, Satoru Yanagisawa, Katsuhiko Yanaga
World Journal of Surgical Oncology , 2012, DOI: 10.1186/1477-7819-10-129
Abstract:
Case Studies of Energy Saving and CO2 Reduction by Cogeneration and Heat Pump Systems  [PDF]
Satoru Okamoto
Open Journal of Energy Efficiency (OJEE) , 2013, DOI: 10.4236/ojee.2013.22014
Abstract:

This paper describes two case studies: 1) a cogeneration system of a hospital and 2) a heat pump system installed in an aquarium that uses seawater for latent heat storage. The cogeneration system is an autonomous system that combines the generation of electrical, heating, and cooling energies in a hospital. Cogeneration systems can provide simultaneous heating and cooling. No technical obstacles were identified for implementing the cogeneration system. The average ratio between electric and thermal loads in the hospital was suitable for the cogeneration system operation. An analysis performed for a non-optimized cogeneration system predicted large potential for energy savings and CO2 reduction. The heat pump system using a low-temperature unutilized heat source is introduced on a heat source load responsive heat pump system, which combines a load variation responsive heat pump utilizing seawater with a latent heat-storage system (ice and water slurry), using nighttime electric power to level the electric power load. The experimental coefficient of performance (COP) of the proposed heat exchanger from the heat pump system, assisted by using seawater as latent heat storage for cooling, is discussed in detail.

Energy-Saving and Economical Evaluations of a Ceramic Gas Turbine Cogeneration Plant  [PDF]
Satoru Okamoto
Open Journal of Energy Efficiency (OJEE) , 2013, DOI: 10.4236/ojee.2013.22012
Abstract:

A ceramic gas turbine can save energy because of its high thermal efficiency at high turbine inlet temperatures. This paper deals with the thermodynamic and economic aspects of a ceramic gas turbine cogeneration system. Here cogeneration means the simultaneous production of electrical en-ergy and useful thermal energy from the same facility. The thermodynamic performance of a ceramic gas turbine cycle is assessed using a computer model. This model is used in parametric studies of performance under partial loads and at various inlet air temperatures. The computed performance is compared to the measured performance of a conventional gas turbine cycle. Then, an economic evaluation of a ceramic gas turbine cogeneration system is investigated. Energy savings provided by this system are estimated on the basis of the distributions of heat/power ratios. The computed economic evaluation is compared to the actual economic performance of a conventional system in which boilers produce the required thermal energy and electricity is purchased from a utility.

On the Application of the Laplace Transform in the Study of Phillips-Type Stabilization Policy  [PDF]
Satoru Kageyama
Theoretical Economics Letters (TEL) , 2015, DOI: 10.4236/tel.2015.56080
Abstract: This paper provides a reformulation of Phillips’s multiplier-accelerator model with stabilization policy in terms of the Laplace transform. Applying the Laplace transform, the differential equations of the economy are transformed into the algebraic ones on a complex variable. The transfer functions of economic variables are defined by these algebraic equations. With this representation, we show the effects of Phillips-type policy on equilibrium level and derive the necessary and sufficient condition for asymptotic stability.
Increase of nitrosative stress in patients with eosinophilic pneumonia
Kanako Furukawa, Hisatoshi Sugiura, Kazuto Matsunaga, Tomohiro Ichikawa, Akira Koarai, Tsunahiko Hirano, Satoru Yanagisawa, Yoshiaki Minakata, Keiichiro Akamatsu, Masae Kanda, Manabu Nishigai, Masakazu Ichinose
Respiratory Research , 2011, DOI: 10.1186/1465-9921-12-81
Abstract: Exhaled NO including fractional exhaled NO (FENO) and Calv was measured in ten healthy subjects, 13 patients with idiopathic pulmonary fibrosis (IPF), and 13 patients with EP. iNOS expression and 3-NT formation were assessed by immunocytochemistory in BALf cells. The exhaled NO, lung function, and systemic inflammatory markers of the EP patients were investigated after corticosteroid treatment for 4 weeks.The Calv levels in the EP group (14.4 ± 2.0 ppb) were significantly higher than those in the healthy subjects (5.1 ± 0.6 ppb, p < 0.01) and the IPF groups (6.3 ± 0.6 ppb, p < 0.01) as well as the FENO and the corrected Calv levels (all p < 0.01). More iNOS and 3-NT positive cells were observed in the EP group compared to the healthy subject and IPF patient. The Calv levels had significant positive correlations with both iNOS (r = 0.858, p < 0.05) and 3-NT positive cells (r = 0.924, p < 0.01). Corticosteroid treatment significantly reduced both the FENO (p < 0.05) and the Calv levels (p < 0.01). The magnitude of reduction in the Calv levels had a significant positive correlation with the peripheral blood eosinophil counts (r = 0.802, p < 0.05).These results suggested that excessive nitrosative stress occurred in EP and that Calv could be a marker of the disease activity.Eosinophilic pneumonia (EP) is an inflammatory lung disease characterized by the infiltration of eosinophils into the alveolar region and interstitium of the lung [1,2]. The accumulation of eosinophils into the lung in EP is reported to be induced by the excessive production of eosinophil chemotactic mediators including interleukin-5 (IL-5) [3,4], IL-18 [5], and granulocyte-macrophage colony-stimulating factor (GM-CSF) [4]. Eosinophils contain a number of preformed mediators and cytotoxic enzymes within cytoplasmic granules [6]. The most abundant preformed substances are major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN), and eosinophil peroxidase (EPO)
Oxidative stress augments toll-like receptor 8 mediated neutrophilic responses in healthy subjects
Satoru Yanagisawa, Akira Koarai, Hisatoshi Sugiura, Tomohiro Ichikawa, Masae Kanda, Rie Tanaka, Keiichiro Akamatsu, Tsunahiko Hirano, Kazuto Matsunaga, Yoshiaki Minakata, Masakazu Ichinose
Respiratory Research , 2009, DOI: 10.1186/1465-9921-10-50
Abstract: Human peripheral blood neutrophils were obtained from healthy non-smokers and stimulated with TLR 7/8 agonist imidazoquinoline resiquimod (R848) in the presence or absence of hydrogen peroxide (H2O2). Neutrophilic responses including cytokine release, superoxide production and chemotaxis were examined, and the signal transduction was also analyzed.Activation of TLR8, but not TLR7, augmented IL-8 release. The R848-augmented IL-8 release was significantly potentiated by pretreatment with H2O2 (p < 0.01), and N-acetyl-L-cysteine reversed this potentiation. The combination of H2O2 and R848 significantly potentiated NF-kB phosphorylation and IkBα degradation. The H2O2-potentiated IL-8 release was suppressed by MG-132, a proteosome inhibitor, and by dexamethasone. The expressions of TLR8, myeloid differentiation primary response gene 88 (MyD88), and tumor necrosis factor receptor-associated factor 6 (TRAF6) were not affected by H2O2.TLR8-mediated neutrophilic responses were markedly potentiated by oxidative stress, and the potentiation was mediated by enhanced NF-kB activation. These results suggest that oxidative stress might potentiate the neutrophilic inflammation during viral infection.Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) and superoxide anion are generated in inflamed tissues and are reported to contribute to the pathogenesis of inflammatory lung diseases including chronic obstructive pulmonary diseases (COPD) [1,2], bronchial asthma [3,4], cystic fibrosis [5,6], and idiopathic pulmonary fibrosis [7,8]. Large amounts of ROS derived from inflammatory cells cause pro-inflammatory cytokine production. In fact, H2O2 has been reported to augment cytokine production in previous studies [9,10]. Among inflammatory cells, neutrophils are a key player in the inflammatory lung diseases. It is well-known that excessive infiltration of neutrophils is observed in the airways during exacerbations induced by viral infections [11-14].Toll-like receptors (TLRs
The Influence of Free 3-Nitrotyrosine and Saliva on the Quantitative Analysis of Protein-Bound 3-Nitrotyrosine in Sputum
Kazuhito Ueshima, Yoshiaki Minakata, Hisatoshi Sugiura, Satoru Yanagisawa, Tomohiro Ichikawa, Keiichirou Akamatsu, Tsunahiko Hirano, Masanori Nakanishi, Kazuto Matsunaga, Toshiyuki Yamagata and Masakazu Ichinose
Analytical Chemistry Insights , 2012,
Abstract: : Background: We have recently developed a new technique for quantitatively measuring protein-bound 3-nitrotyrosine (3-NT), a footprint of nitrosative stress, utilizing high-performance liquid chromatography with an electrochemical detection (HPLC-ECD) system. Using this system, we showed that 3-NT formation was upregulated in the sputum of both COPD and asthmatic patients. However, in order to improve the accuracy of the measurement system. We have to resolve some problems which were the influence of free amino acid form of 3-NT and of salivary contamination. Objectives: We initially investigated the amount of the free amino acid form of 3-NT in induced sputum and compared with that of protein-bound 3-NT. Next, we evaluated the concentration of protein-bound 3-NT in saliva and compared with that in induced sputum by means of HPLC-ECD. Methods: Five male COPD patients were enrolled. Induced sputum and saliva were obtained from the patients. The free amino acid form of 3-NT in sputum and saliva was measured by HPLC-ECD, and the protein-bound 3-NT and tyrosine in sputum and saliva were enzymatically hydrolyzed by Streptomyces griseus Pronase and measured for the protein hydrolysate by HPLC-ECD. Results: The mean value of the amount of protein-bound 3-NT was 65.0 fmol (31.2 to 106.4 fmol). On the other hand, the amount of the free amino acid form of 3-NT was under the detection limit (<10 fmol). The levels of both 3-NT (sputum: 0.55 ± 0.15 pmol/ml, saliva: 0.02 ± 0.01 pmol/ml, p < 0.01) and tyrosine (sputum: 0.81 ± 0.43 μmol/ml, saliva: 0.07 ± 0.04 μmol/ml, p < 0.01) in saliva were significantly lower than in sputum. The percentage of 3-NT in saliva to that in sputum was about 3.1%, and that of tyrosine was about 9.0%. Conclusion: The free amino acid form of 3-NT does not affect the measurement of protein-bound 3-NT. Furthermore, the influence of salivary contamination on the measurement of protein-bound 3-NT in induced sputum by means of HPLC-ECD was very small and could be negligible.
The Decline of Village Common Lands and Changes in Village Society: South India, c. 1850-2000
Yanagisawa Haruka
Conservation & Society , 2008,
Abstract: The widely accepted view that emphasises the negative impact of the decline in common property re-sources on the village poor generally presumes that village common lands would have been used by all villagers inclusive of the poor without serious differences in the right to access them. Mainly based on historical documents for Tamil Nadu from the nineteenth century, this paper argues that influential vil-lagers controlled ′waste lands′ (village common land) and that this elite-dominant system of controlling natural resources declined with the gradual emancipation of the subordinate section of villagers. The ac-quisition of small bits of cultivated land and the encroachment on waste lands by the landless not only mirrored their empowerment and strengthened their bargaining position but also implies, under some cir-cumstances, the creation of possible pre-conditions for an egalitarian type of resource-controlling system. This paper also suggests that, as witnessed in Tamil Nadu in the last two decades, the growth of non-agricultural job opportunities could possibly weaken the pressure on lands and also induce farmers to change cropping patterns of their farms, sometimes leading to an expansion of farm forestry. The acquisi-tion of landholding by the landless and their emancipation could also possibly contribute in this direction.
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