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Search Results: 1 - 6 of 6 matches for " Sarinya Sirisate "
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Homocysteine, Vitamin B12 and Folic Acid in Children with Acute Glomerulonephritis  [PDF]
Duangkamol Viroonudomphol, Saowanee Kanjanachumpol, Sarinya Sirisate
World Journal of Engineering and Technology (WJET) , 2018, DOI: 10.4236/wjet.2018.63042
Abstract: Homocysteine (Hcy) is an intermediate product of methionine formed by its demethylation. Hcy can be metabolized via remethylation to methionine or transsulfuration to cysteine which is dependent on several enzymes and cofactors. It is deleterious to blood vessel including glomeruli. Kidney is a major organ that metabolizes Hcy. More than 80% of patients with chronic renal disease develop hyperhomocysteinemia (hHcy). Accessible data of plasma Hcy in nephritic syndrome (NS) patients are controversial with increased, decreased and unchanged values reported. In renal patients, plasma Hcy concentration can be reduced by administration of folic acid. Absolute or relative deficiencies of folate, vitamin B6, or vitamin B12 may also play a role. Therefore, plasma Hcy, folic acid, vitamin B6, and vitamin B12 in children with acute glomerulonephritis (AGN) were accessed in this study. Hcy, folic acid vitamin B12, B6 and renal function such as blood urea nitrogen (BUN), creatinine (Cr) were analyzed 12 pediatric patients with AGN and 15 age and sex matched healthy children served as controls. The results revealed that a?significant increase in plasma Hcy in children with acute AGN when compared with controls. For simple regression analysis, Hcy was positively correlated with BUN, Cr, ferritin and uric acid but negatively correlated with serum glutathione. This research indicated hHcy suggests enhanced risks for inflammation and endothelial injury,?which lead to kidney disease. Folic acid has also been shown to improve endothelial function, suggesting an alternative explanation for the effect of folic acid on endothelial function. Careful considerations of not only dietary measures are necessary but also folate and vitamin B supplementation for reducing hHcy in AGN need to be investigated.
Inhibitory Activities of Cyanidin and Its Glycosides and Synergistic Effect with Acarbose against Intestinal α-Glucosidase and Pancreatic α-Amylase
Sarinya Akkarachiyasit,Piyawan Charoenlertkul,Sirintorn Yibchok-anun,Sirichai Adisakwattana
International Journal of Molecular Sciences , 2010, DOI: 10.3390/ijms11093387
Abstract: Cyanidin and its glycosides are naturally dietary pigments which have been indicated as promising candidates to have potential benefits to humans, especially in the prevention and treatment of diabetes mellitus. We investigated the structure activity relationships of cyanidin and its glycosides to inhibit intestinal a-glucosidases and pancreatic a-amylase in vitro. The results found that cyanidin and its glycosides are more specific inhibitors of intestinal sucrase than intestinal maltase. Cyanidin-3-galactoside and cyanidin-3-glucoside were the most potent inhibitors against intestinal sucrase and pancreatic a-amylase with IC 50 values of 0.50?±?0.05 and 0.30?± 0.01 mM, respectively. Our findings indicate that the structural difference between glucose and galactose at the 3- O-position of cyanidin was an important factor for modulating the inhibition of intestinal sucrase and pancreatic a-amylase. The combination of cyandin-3-glucoside, cyanidin-3-galactoside or cyanidin-3,5-diglucosides with a low concentration of acarbose showed synergistic inhibition on intestinal maltase and sucrase. The synergistic inhibition was also found for a combination of cyanidin or cyanidin-3-glucoside with a low concentration of acarbose. The findings could provide a new insight into a use for the naturally occurring intestinal a-glucosidase and pancreatic a-amylase inhibitors for the prevention and treatment of diabetes and its complications.
In Vitro Inhibitory Effects of Cyandin-3-rutinoside on Pancreatic α-Amylase and Its Combined Effect with Acarbose
Sarinya Akkarachiyasit,Sirintorn Yibchok-Anun,Sumrit Wacharasindhu,Sirichai Adisakwattana
Molecules , 2011, DOI: 10.3390/molecules16032075
Abstract: The inhibitory activity on pancreatic α-amylase by cyanidin-3-rutinoside was examined in vitro. The IC50 value of cyanidin-3-rutinoside against pancreatic α-amylase was 24.4 ± 0.1 μM. The kinetic analysis revealed that pancreatic α-amylase was inhibited by cyanidin-3-rutinoside in a non-competitive manner. The additive inhibition of a combination of cyanidin-3-rutinoside with acarbose against pancreatic α-amylase was also found. These results provide the first evidence for the effect of cyanidin-3-rutinoside in a retarded absorption of carbohydrates by inhibition of pancreatic α-amylase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.
The Participation of Community Activities in Child Caring in the Community Child-Care Centres in Northeast Thailand
Yodkhwan Gedthongma,Chaloem Varavithya,Sarinya Srisarakham,Manop Kanato
Pakistan Journal of Social Sciences , 2012, DOI: 10.3923/pjssci.2010.112.116
Abstract: The objectives of the study are to develop and compare the participation of the community activities in the community child-care centres between the municipality and the local administration organization. This research is an action research, which studied during the months of February, 1998-March, 1999. The informants are composed of parents and the related persons. To collect and analyse the data by qualitative and quantitative research methods. The finding of the study were that the persons who have active role in child caring in the child-care centres included: children s family; child-care centres; the related organizations and volunteers who develop child-care centre. Moreover, there were some groups that engage in sub-activities. In the area of the municipality, the sub-activities were the child development supporting activities, food and nutrition activities, healthy young child activities, beautiful house activities and warm child-care centre. In the area of the local administrative organization there were the following activities: beautiful golden mountain activities teen-age development activities and good nutritional activities. When comparing the activities of both areas, they were both similar. However, there were some differences in the quantity, frequency, duration of time and the participation in activities styles. It was found that in the municipality area of child-care, there were more participants joining the activities. More participants attended the activities more often and there was more time spent on proceeding the activities. The participation was more formal. While the child-care of the local administrative office was a simple, in the folk way approach. These activities made everyone accept the child-care centres. It was efficiently found that there were follow-up development: the parents believes and child development.
Dicoumarol enhances gemcitabine-induced cytotoxicity in high NQO1-expressing cholangiocarcinoma cells
Benjaporn Buranrat, Auemduan Prawan, Upa Kukongviriyapan, Sarinya Kongpetch, Veerapol Kukongviriyapan
World Journal of Gastroenterology , 2010,
Abstract: AIM: To investigate whether dicoumarol, a potent inhibitor of NAD(P)H quinone oxidoreductase-1 (NQO1), potentiates gemcitabine to induce cytotoxicity in cholangiocarcinoma cells (CCA) and the role of reactive oxygen generation in sensitizing the cells.METHODS: Four human cell lines with different NQO1 activity were used; the human CCA cell lines, KKU-100, KKU-OCA17, KKU-M214, and Chang liver cells. NQO1 activity and mRNA expression were determined. The cells were pretreated with dicoumarol at relevant concentrations before treatment with gemcitabine. Cytotoxicity was determined by staining with fluorescent dyes. Oxidant formation was examined by assay of cellular glutathione levels and reactive oxygen species production by using dihydrofluorescein diacetate. Measurement of mitochondrial transmembrane potential was performed by using JC-1 fluorescent probe. Western blotting analysis was performed to determine levels of survival related proteins.RESULTS: Dicoumarol markedly enhanced the cytotoxicity of gemcitabine in KKU-100 and KKU-OCA17, the high NQO1 activity and mRNA expressing cells, but not in the other cells with low NQO1 activity. Dicoumarol induced a marked decrease in cellular redox of glutathione in KKU-100 cells, in contrast to KKU-M214 cells. Dicoumarol at concentrations that inhibited NQO1 activity did not alter mitochondrial transmembrane potential and production of reactive oxygen species. Gemcitabine alone induced activation of NF-κB and Bcl-XL protein expression. However, gemcitabine and dicoumarol combination induced increased p53 and decreased Bcl-XL levels in KKU-100, but not in KKU-M214 cells.CONCLUSION: NQO1 may be important in sensitizing cells to anticancer drugs and inhibition of NQO1 may be a strategy for the treatment of CCA.
Crucial Role of Heme Oxygenase-1 on the Sensitivity of Cholangiocarcinoma Cells to Chemotherapeutic Agents
Sarinya Kongpetch, Veerapol Kukongviriyapan, Auemduan Prawan, Laddawan Senggunprai, Upa Kukongviriyapan, Benjaporn Buranrat
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034994
Abstract: Cancer cells acquire drug resistance via various mechanisms including enhanced cellular cytoprotective and antioxidant activities. Heme oxygenase-1 (HO-1) is a key enzyme exerting potent cytoprotection, cell proliferation and drug resistance. We aimed to investigate roles of HO-1 in human cholangiocarcinoma (CCA) cells for cytoprotection against chemotherapeutic agents. KKU-100 and KKU-M214 CCA cell lines with high and low HO-1 expression levels, respectively, were used to evaluate the sensitivity to chemotherapeutic agents, gemcitabine (Gem) and doxorubicin. Inhibition of HO-1 by zinc protoporphyrin IX (ZnPP) sensitized both cell types to the cytotoxicity of chemotherapeutic agents. HO-1 gene silencing by siRNA validated the cytoprotective effect of HO-1 on CCA cells against Gem. Induction of HO-1 protein expression by stannous chloride enhanced the cytoprotection and suppression of apoptosis caused by anticancer agents. The sensitizing effect of ZnPP was associated with increased ROS formation and loss of mitochondrial transmembrane potential, while Gem alone did not show any effects. A ROS scavenger, Tempol, abolished the sensitizing effect of ZnPP on Gem. Combination of ZnPP and Gem enhanced the release of cytochrome c and increased p21 levels. The results show that HO-1 played a critical role in cytoprotection in CCA cells against chemotherapeutic agents. Targeted inhibition of HO-1 may be a strategy to overcome drug resistance in chemotherapy of bile duct cancer.
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