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Search Results: 1 - 10 of 23989 matches for " Sanjay Kumar Yadav "
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Genetic Diversity of Pigeonpea (Cajanus cajan (L.) Millsp.) Cultivars and Its Wild Relatives Using Randomly Amplified Polymorphic DNA (RAPD) Markers  [PDF]
Kusum Yadav, Sanjay Kumar Yadav, Anurag Yadav, Veda Prakash Pandey, Upendra Nath Dwivedi
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.33038
Abstract: Genetic diversity among and between 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its wild relatives (C. albicans and C. lineatus) analysed using RAPD. Twenty two random primers with an average of 71.2% polymorphism produced 151 polymorphic bands. Cluster analysis based on these 151 RAPD markers revealed relatively low level (0.434 - 0.714) of genetic diversity among cultivars and high level of diversity between cultivars and wild relatives. C. albicans and C. lineatus showed only 0.231 similarity with each other and C. albicans showed relatively higher similarity with C. cajan cultivars than that showed by C. lineatus. In dendrogram the 16 cultivars grouped into two distinct clusters comprising of seven and nine genotypes each while the wild species form out groups. Bootstrap analysis of the dendrogram was performed and resulted in significant bootstrap values. Principal components analysis (PCA) also revealed the similar results that of unweighted pair group method with arithmetic mean (UPGMA). The first, second and third PCs contributed 55.9%, 5.9%, and 5.6% of the variation, respectively, with cumulative variation of the first three PCs was 67.4%.
Comparative Analysis of Genetic Diversity among Cultivated Pigeonpea (Cajanus cajan (L) Millsp.) and Its Wild Relatives (C. albicans and C. lineatus) Using Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) Fingerprinting  [PDF]
Kusum Yadav, Sanjay Kumar Yadav, Anurag Yadav, Veda Prakash Pandey, Upendra Nath Dwivedi
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.511181

Genetic relationships of 16 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) and its two wild relatives (C. albicans and C. lineatus) from different parts of the India were analysed using 22 random amplified polymorphic DNAs (RAPDs) primers and 10 inter simple sequence repeats (ISSRs) primers. Twenty two RAPD primers yielded 151 polymorphic markers (71.2%) with an average of 6.8 polymorphic band/primer. Cluster analysis based on these 151 RAPD markers revealed relatively low level (0.434 - 0.714) of genetic diversity among cultivars and high level of diversity between cultivars and wild relatives. Ten ISSR primers produced 100 bands across 16 cultivars and its wild relatives out of which 93 (93%) were polymorphic with an average of 9.3 polymorphic band/primer. Cluster analysis based on these 93 ISSR markers also revealed relatively higher level (0.328 - 0.827) of genetic diversity among cultivars as compared to RAPD markers. The polymorphic markers obtained by both RAPD and ISSR primers were pooled and the genetic diversity analysis based on these 244 markers was analysed. Jaccard’s similarity coefficient obtained by pooled data revealed very narrow range (0.477 - 0.720) among cultivated and high range between cultivated and wild species C. albicans (0.240 - 0.331) and C. lineatus (0.163 - 0.193). In the UPGMA based dendrogram the 16 cultivars were grouped into three distinct clusters. Cluster I contained two genotypes, cluster II had six and cluster III had eight genotypes. Principal components analysis (PCA) also resulted in similar pattern as that of UPGMA based analysis. The first three PCs contributed 56.26%, 5.71% and 4.97% of variation, respectively, with cumulative variation of the first three PCs was 66.96%. Both the markers and the combined data revealed similar pattern with narrow diversity among cultivars and higher diversity between cultivars and wild one, but

Experimental Investigation of Centrifugal Pump Working as Turbine for Small Hydropower Systems
Himanshu Nautiyal,Varun Varun,Anoop Kumar,Sanjay Yadav Sanjay Yadav
Energy Science and Technology , 2011,
Abstract: An experimental investigation of centrifugal pump has been carried out to study its characteristics in pump and turbine mode operation. By using the experimental results of tested pump and pumps of some previous researchers, new correlations have been developed by using its best efficiency and specific speed in pump mode. Values obtained from the derived correlations show good match with experimental results. These correlations would be very helpful for the performance prediction of pump working as turbine. Key words: PAT; Centrifugal pump; Specific speed; Best efficiency point
Indigenous knowledge of medicinal plants used by Saperas community of Khetawas, Jhajjar District, Haryana, India
Manju Panghal, Vedpriya Arya, Sanjay Yadav, Sunil Kumar, Jaya Yadav
Journal of Ethnobiology and Ethnomedicine , 2010, DOI: 10.1186/1746-4269-6-4
Abstract: Ethno botanical data and traditional uses of plants information was obtained by semi structured oral interviews from experienced rural folk, traditional herbal medicine practitioners of the 'Nath' community. A total of 42 selected inhabitants were interviewed, 41 were male and only one woman. The age of the healers was between 25 years and 75 years. The plant specimens were identified according to different references concerning the medicinal plants of Haryana and adjoining areas and further confirmation from Forest Research Institute, Dehradun.The present study revealed that the people of the snake charmer community used 57 medicinal plants species that belonged to 51 genera and 35 families for the treatment of various diseases. The study has brought to light that the main diseases treated by this community was snakebite in which 19 different types of medicinal plants belongs to 13 families were used. Significantly higher number of medicinal plants was claimed by men as compared to women. The highest numbers of medicinal plants for traditional uses utilized by this community were belonging to family Fabaceae.This community carries a vast knowledge of medicinal plants but as snake charming is banned in India as part of efforts to protect India's steadily depleting wildlife, this knowledge is also rapidly disappearing in this community. Such type of ethno botanical studies will help in systematic documentation of ethno botanical knowledge and availing to the scientific world plant therapies used as antivenin by the Saperas community.Utilization of plants for medicinal purposes in India has been documented long back in ancient literature because they are essential to human survival [1,2]. The consumption, management and valuation of wild plants are central aspects of the traditional knowledge in many human populations. Thus, plants gathering, the diffusion and conservation of knowledge within the community are traditional practices that have contribution to the subsis
Transgenic Tobacco Overexpressing Tea cDNA Encoding Dihydroflavonol 4-Reductase and Anthocyanidin Reductase Induces Early Flowering and Provides Biotic Stress Tolerance
Vinay Kumar, Gireesh Nadda, Sanjay Kumar, Sudesh Kumar Yadav
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0065535
Abstract: Flavan-3-ols contribute significantly to flavonoid content of tea (Camellia sinensis L.). Dihydroflavonol 4-reductase (DFR) and anthocyanidin reductase (ANR) are known to be key regulatory enzymes of flavan-3-ols biosynthesis. In this study, we have generated the transgenic tobacco overexpressing individually tea cDNA CsDFR and CsANR encoding for DFR and ANR to evaluate their influence on developmental and protective abilities of plant against biotic stress. The transgenic lines of CsDFR and CsANR produced early flowering and better seed yield. Both types of transgenic tobacco showed higher content of flavonoids than control. Flavan-3-ols such as catechin, epicatechin and epicatechingallate were found to be increased in transgenic lines. The free radical scavenging activity of CsDFR and CsANR transgenic lines was improved. Oxidative stress was observed to induce lesser cell death in transgenic lines compared to control tobacco plants. Transgenic tobacco overexpressing CsDFR and CsANR also showed resistance against infestation by a tobacco leaf cutworm Spodoptera litura. Results suggested that the overexpression of CsDFR and CsANR cDNA in tobacco has improved flavonoids content and antioxidant potential. These attributes in transgenic tobacco have ultimately improved their growth and development, and biotic stress tolerance.
Evaluation of In-Vitro Antioxidant Activity in Ficus religiosa (L.) Leaves
Kumar Hemant,Goswami Mradul,Yadav Sanjay,Rao Ch. V
International Journal of Research in Pharmacy and Science , 2011,
Abstract: Medicinal plants have been used in traditional medicine for the treatment of several diseases. In India, medicines based on herbal origin have been the basis of treatment and cure for various diseases and physiological abnormalities under practice such as Ayurveda, Siddha and Unani. Research in herbal medicine has recently been revolutionized with the identification of several botanical plants with established physiological effect and efficacy for clinical condition either alone or combination with pharmaceuticals. Ayurveda is a comprehensive natural health care system that organized in India more than 5000 year ago. Plant antioxidants are composed of a broad variety of different substances like ascorbic acid and tocopherols, polyphenolic compounds, or terpenoids. They perform several important functions in plants and humans. In this study, antioxidant activity of ethanolic extract of Ficus religiosa Linn. (EEFR) leaf was investigated for its free radical scavenging activity by adopting various in vitro models. The extract was investigated for its antioxidant activity by 1,1--diphenyl, 2-picryl hydrazyl (DPPH) radical scavenging activity, hydroxyl radical scavenging activity, reducing capacity, hydrogen peroxide activity, determination of total phenolic content using Folin-Ciocalteu's phenolic reagent. EEFR showed maximum scavenging of DDPH radical 91.20% at 250 μg/ml concentration and hydrogen peroxide and reducing power were also dose dependent. The IC50 values were found to be 71.10μg/ml and 22.5 μg/ml of EEFR and ascorbic acid respectively. The total phenolic content evaluated that 1 mg of extract contained 3.2μg Gallic acid equivalents of phenols respectively. The extract showed significant results when compared with standard groups.
Water Quality Index Assessment ofGroundwater in Todaraisingh Tehsil of Rajasthan State, India-A Greener Approach
Ashok Kumar Yadav,Parveen Khan,Sanjay K. Sharma
Journal of Chemistry , 2010, DOI: 10.1155/2010/419432
Microorganisms and Their Sensitivity Pattern in Septic Arthritis of North Indian Children: A Prospective Study from Tertiary Care Level Hospital
Sanjay Yadav,Mandeep Singh Dhillon,Sameer Aggrawal,Sujit Kumar Tripathy
ISRN Orthopedics , 2013, DOI: 10.1155/2013/583013
Abstract: Background. Septic arthritis is a true orthopaedic emergency. Important factors determining outcome are rapid diagnosis and timely intervention. Changing trends in microbiological spectrum and emerging drug resistance poses big challenge. Present study evaluates bacterial strains and their sensitivity pattern in septic arthritis of North Indian children. Methods. Fifty children with septic arthritis of any joint were evaluated. Joint was aspirated and 2?cc of aspirated fluid was sent for gram stain and culture. Blood cultures were also sent for bacteriological evaluation. Results. Fifty percent cases had definite radiological evidence of septic arthritis whereas ultrasound revealed fluid in 98% cases. Aspirated fluid showed isolates in 72% cases. The most common organism was Staphylococcus aureus (62%) followed by Streptococcus pneumoniae and Gr. B Streptococcus. Blood culture could grow the organism in 34% cases only. The bacterial strain showed significant resistance to common antibiotic cocktail in routine practice. Resistance to cloxacillin and ceftriaxone was 62% and 14% respectively. No organisms were resistant to vancomycin and linezolid. Conclusion. S. aureus is still the most common organism in septic arthritis. Though a significant resistance to common antibiotic cocktail is noticed, the strain is susceptible to higher antibiotics. We recommend using these antibiotics as an empirical therapy till culture and sensitivity report is available. 1. Introduction Septic arthritis is a true orthopaedic emergency. Delay in its diagnosis and treatment can lead to disastrous complications like destruction of articular cartilage, physeal damage, and dislocation of joints (Figure 1) [1–3]. Despite significant improvement in medicine with availability of better antibiotics, septic arthritis is still a major cause of morbidity. The cause is multifactorial as there is a shift in the microbiological spectra and epidemiology with emerging antibiotic resistance. This also has a distinct geographical variation [4–8]. Figure 1: Septic arthritis causing pathological dislocation. Native joint septic arthritis is usually secondary to hematogenous seeding of joint during transient or persistent bacteraemia [1, 2, 9]. Early treatment is essential before damage to the articular cartilage occurs. This mandates empirical antibiotic therapy without awaiting culture report [1, 2, 4]. Because of wide variation in microbiological spectrum and their sensitivity, a constant antibiotic regimen cannot be designed for all children. Accordingly, this prospective study was conducted
Phosphate solubilizing ability of two Arctic Aspergillus niger strains
Shiv Mohan Singh,,Lal Sahab Yadav,,Sanjay Kumar Singh,,Purnima Singh
Polar Research , 2011, DOI: 10.3402/polar.v30i0.7283
Abstract: Many filamentous fungi were isolated from the soils of Ny- lesund, Spitsbergen, Svalbard, and were screened in vitro for their phosphate solubilizing ability. Two strains of Aspergillus niger showed good tricalcium phosphate (TCP) solubilizing ability in Pikovskaya's medium. The TCP solubilization index was calculated at varying levels of pH and temperatures. The ability of Aspergillus niger strain-1 to solubilize and release inorganic-P was 285 μg ml–1, while Aspergillus niger strain-2 solubilized 262 μg ml–1 from 0.5% TCP after seven days. This is the first report of TCP solubilization by Arctic strains that may serve as very good phosphate solubilizers in the form of biofertilizer.
Synthesis of Oxovanadium(IV) Complexes with Tetraaza Coordinating Ligands
Sanjay Singh,Hardeo S. Yadav,Ashok Kumar Yadava,Devendra Pratap Rao
Journal of Chemistry , 2013, DOI: 10.1155/2013/947325
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