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Search Results: 1 - 10 of 410761 matches for " Samier M. El-Awady "
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Tei Index versus Pro BNP as Predictors of Weaning Failure in Mechanically Ventilated Chronic Obstructive Pulmonary Disease Patients  [PDF]
Hesham M. El-Ashmawy, Tamer A. Helmy, Samier M. El-Awady, Doaa Hashad, Ahmed M. Nabil
Open Journal of Respiratory Diseases (OJRD) , 2015, DOI: 10.4236/ojrd.2015.53007
Abstract: Objective: This prospective cohort study was designed to assess the rule of myocardial performance (Tei) index and NT-Pro BNP serum levels in prediction of weaning failure in mechanically ventilated COPD patients. Methods: Forty patients with respiratory failure due to acute exacerbation of COPD were enrolled to this study. All patients underwent a comprehensive echocardiographic examination including tissue doppler imaging within first 12 hours of admission. NT-pro BNP serum levels were measured on admission. Patients were followed up for outcome of weaning failure. Results: Right ventricular Tei index was higher in patients with failed weaning in comparison to successfully weaned patients (mean 0.66 versus 0.36, P < 0.001), also left ventricular Tei index was higher in patients who failed to be weaned (mean 0.45 versus 0.36, P = 0.007). There was no difference in serum NT-Pro BNP between successfully weaned patients and patients with weaning failure. Right ventricular Tei index showed higher accuracy in predicting weaning failure than the left ventricular Tei index (AUC 0.932 versus 0.754), while serum NT-Pro BNP showed no discrimination in predicting weaning failure (AUC 0.556). Conclusion: Tei index was superior to NT-ProBNP in prediction of weaning failure in COPD patients.
Combination of Paracetamol and the Glutathione Depleting Agent Buthionine Sulfoximine Show Differential Effect on Liver Cancer Cells and Normal Hepatocytes  [PDF]
Marwa E. Sayour, Rania M. Abd El Salam, Mohamed F. Elyamany, Abeer M. El Sayed, Raafat A. El-Awady
Pharmacology & Pharmacy (PP) , 2016, DOI: 10.4236/pp.2016.711051
Abstract: Background: Paracetamol exerts toxic effects on liver cells through its metabolism into N-acetyl-p-benzoquinone imine (NAPQI), which is detoxified by conjugation with cellular glutathione (GSH). Once GSH is depleted, NAPQI stimulates a range of oxidative reactions that result in cell necrosis. The aim of the present investigation is to find a new strategy that would selectively protect normal hepatic tissues and sensitize liver cancer cells to the toxic effects of paracetamol or its metabolite. This may lead to the development of a targeted therapy for liver cancer. Methods: The anti-proliferative effects of paracetamol and buthionine sulfoximine BSO (a glutathione depleting agent) alone and in combination on the liver cancer cells HepG2 and normal rat hepatocytes were investigated by sulphorhodamine-B assay. Effects on cell cycle regulation and induction of apoptosis were tested by flow cytometry. The level of prostaglandin expression was measured by ELISA. Results: The present study showed that both agents alone or in combination have anti-proliferative effects on both cell types. Surprisingly, BSO showed a cytoprotective effects on normal hepatocytes treated with high concentrations (1.75 and 2 mM) of paracetamol. This was confirmed by cell cycle analysis that recorded decreased fraction of sub-G1 cells indicating reduction of apoptosis in normal hepatocytes. Analysis of prostaglandin E2 revealed differential effects of paracetamol on normal and liver cancer cells. A significant increase in PGE2 level over the control was observed in normal hepatocytes whereas a significant decrease was seen in HepG2 cells after treatment with paracetamol. Conclusion: These results indicate that combination of paracetamol/BSO has differential effects on liver cancer cells and normal hepatocytes, which opens the avenue for a new effective and selective combination for management of liver cancer.
IRIS RECOGNITION BASED ON LBP AND COMBINED LVQ CLASSIFIER
M. Z. Rashad,M. Y. Shams,O. Nomir,R. M. El-Awady
International Journal of Computer Science & Information Technology , 2011,
Abstract: Iris recognition is considered as one of the best biometric methods used for human identification andverification, this is because of its unique features that differ from one person to another, and itsimportance in the security field. This paper proposes an algorithm for iris recognition and classificationusing a system based on Local Binary Pattern and histogram properties as a statistical approaches forfeature extraction , and Combined Learning Vector Quantization Classifier as Neural Network approachfor classification, in order to build a hybrid model depends on both features. The localization andsegmentation techniques are presented using both Canny edge detection and Hough Circular Transformin order to isolate an iris from the whole eye image and for noise detection .Feature vectors results fromLBP is applied to a Combined LVQ classifier with different classes to determine the minimum acceptableperformance, and the result is based on majority voting among several LVQ classifier. Different irisdatasets CASIA, MMU1, MMU2, and LEI with different extensions and size are presented. Since LBP isworking on a grayscale level so colored iris images should be transformed into a grayscale level. Theproposed system gives a high recognition rate 99.87 % on different iris datasets compared with othermethods.
Iris Recognition Based on LBP and Combined LVQ Classifier
M. Y. Shams,M. Z. Rashad,O. Nomir,R. M. El-Awady
Computer Science , 2011, DOI: 10.5121/ijcsit.2011.3506
Abstract: Iris recognition is considered as one of the best biometric methods used for human identification and verification, this is because of its unique features that differ from one person to another, and its importance in the security field. This paper proposes an algorithm for iris recognition and classification using a system based on Local Binary Pattern and histogram properties as a statistical approaches for feature extraction, and Combined Learning Vector Quantization Classifier as Neural Network approach for classification, in order to build a hybrid model depends on both features. The localization and segmentation techniques are presented using both Canny edge detection and Hough Circular Transform in order to isolate an iris from the whole eye image and for noise detection .Feature vectors results from LBP is applied to a Combined LVQ classifier with different classes to determine the minimum acceptable performance, and the result is based on majority voting among several LVQ classifier. Different iris datasets CASIA, MMU1, MMU2, and LEI with different extensions and size are presented. Since LBP is working on a grayscale level so colored iris images should be transformed into a grayscale level. The proposed system gives a high recognition rate 99.87 % on different iris datasets compared with other methods.
Selection Indexes for Genetic Improvement of Preweaning Growth Traits in Friesin Calves in Egypt
E.Z.M. Oudah,H.G. El-Awady
Pakistan Journal of Biological Sciences , 2006,
Abstract: Records of Birth Weight (BWT) and Weaning Weight (WWT) of 1184 Friesian cattle calves (591 male and 593 female), progeny of 49 sires during the period from 1990 to 2000 were analyzed. The objective of this study was to construct selection indexes for selecting Friesian calves on the basis of pre-weaning growth traits included birth weight, weaning weight and Average Daily Gain (ADG) from birth to weaning. The data were analyzed using Least Square Maximum Likelihood Computer Program. Heritability estimates (±SE) were 0.24±0.08, 0.28±0.08 and 0.28±0.08, for BWT, WWT and ADG, respectively. The genetic and phenotypic correlations between WWT and ADG were high and positive (0.907 and 0.892, respectively), while the corresponding correlations between BWT and ADG were low and negative (-0.285 and -0.278, respectively). Four selection indexes were constructed. Index 1 = -1.029 BWT+1.459 WWT -1.207 ADG, index 2 = 0.2201 BWT+0.2077 WWT, index 3 = 0.2426 BWT+0.0991 ADG and the index 4 = 0.1525 WWT+0.0960 ADG. The correlation between the index and the aggregate genotype varied between 0.50 and 0.52. Index 1 which incorporate all traits was the best (RIH = 0.52) and through this index could be improve all traits together. The present results indicated that genetic improvement for pre-weaning growth traits of Friesian calves could be achieved through multiple trait selection indexes.
Effect of Milk Yield on Economic Profitability of Holstein Friesian Cows under Intensive Production System in Egypt
H. G. El-Awady
Pakistan Veterinary Journal , 2013,
Abstract: Data of productive and reproductive traits of 1961 of lactation records were obtained from 763 Dutch Friesian cows grew up at Wadi El-Sharkia farm, El-Salheia, Egypt. These data were analyzed to empirical comparison between the profitability of three herds under intensive production system in Egypt. The second herd (H2) produced average milk yield of 8619 kg/lactation, followed by first herd (H1) (5138 kg/lactation) which was lower than the H2 by at least one standard deviation (SD = 2935 kg). The third herd (H3) produced high milk yield equal to the average of H2 plus at least one standard deviation. Traits studied were milk yield per day (M/D, kg), total milk yield (TMY, kg), lactation period (LP, day), dry period (DP, day), annual milk yield (AMY, kg), days open (DO, day), number of services per conception (NSPC), calving interval (CI, day), number of lactation completed (NLC) and age at first calving (AFC). Least squares analysis of variance showed highly significant (P<0.01) effects of all factors on all traits studied except the effect of month and year of calving on NSPC that was non-significant. For comparing between the three herds, the deterministic model was used to estimate the annual gross margin and benefit/cost ratio as economic parameters. Prices of inputs and outputs were based on market and farm gate prices during the period from 1998 to 2007. DO, CI and NSPC were increased in H3 vs. H2 and H1, indicating poorer reproductive efficiency of high yielding herd. Moreover, H3 gave 1.03 and 1.5 parity less for each cow than that of H2 and H1, respectively. Economic evaluations indicated that the annual variable cost were (LE) (Egyptian pound = 0.17 USD and = 0.13 EUR) 5136, 6910 and 7845 of H1, H2 and H3, respectively. However, the annual gross margin of H3 was higher than that of H1 and H2 by 79 and 24%, respectively, and the benefit/cost ratio of H3 was 1.90 relative to 1.63 and 1.68 for H1 and H2, respectively. The profit per cow during the lifetime production of H3 was 72 and 19.04% more than of H1 and H2, respectively. It is concluded that under intensive production system, extension of calving interval for high yielding herds seem more profitable than the herds that have shorter calving interval and lactation period.
Genetic distance and heterogenecity between quasispecies is a critical predictor to IFN response in Egyptian patients with HCV genotype-4
Abdel Zekri, Hanaa El-Din, Abeer A Bahnassy, Mohsen M Khaled, Ashraf Omar, Inas Fouad, Mahmoud El-Hefnewi, Fouad Thakeb, Mostafa El-Awady
Virology Journal , 2007, DOI: 10.1186/1743-422x-4-16
Abstract: HCV genotype 4 quasispecies diversity within the 5' untranslated region (5'UTR) was studied in a series of 22 native Egyptian patients with chronic hepatitis C virus with no previous treatment who satisfied all NIH criteria for combined treatment of pegylated IFN and ribavirine and was correlated with the outcome of treatment. The study also included 7 control patients with no antiviral treatment. HCV sequencing was done using the TRUGENE HCV 5-NC genotyping kit.At the 48th week of treatment, 15 patients (68%) showed virological response. Whereas HCV-RNA was still detected in 7 patients (32%) in this period; of those, 6 experienced a partial virological response followed by viral breakthrough during treatment. Only one patient did not show any virological or chemical response. The four females included in this study were all responders. There was a significant correlation between the response rate and lower fibrosis (p = 0.026) as well as the total number of mutation spots (including all the insertions, deletions, transitions and transversions) (p = 0.007, p = 0.035).Patients who responded to interferon treatment had statistically significant less number in both transitions (p = 0.007) and the genetic distances between the quasispecies (p = 0.035). So, viral genetic complexity and variability may play a role in the response to IFN treatment. The consensus alignment of all three groups revealed no characteristic pattern among the three groups. However, the G to A transitions at 160 was observed among non responders who need further study to confirm this observation.Infection with hepatitis C virus (HCV) is a leading cause of chronic liver disease worldwide [1]. Despite recent success after the introduction of combination therapy with IFN-α and Ribavirin, about 60% of patients with HCV genotype 4 fail to respond [2,3]. Resistance to antiviral therapy remains a serious problem in the management of chronic hepatitis C.The basis of treatment of chronic hepatitis C is int
Soluble egg antigen of Schistosoma Haematobium induces HCV replication in PBMC from patients with chronic HCV infection
Mostafa K El-Awady, Samar S Youssef, Moataza H Omran, Ashraf A Tabll, Wael T El Garf, Ahmed M Salem
BMC Infectious Diseases , 2006, DOI: 10.1186/1471-2334-6-91
Abstract: PBMC from 26 patients with chronic HCV infection were cultured for 72 hours in presence and absence of 50 μg SEA/ml medium. Intracellular HCV RNA quantification of plus and minus strands was assessed before and after stimulation. PBMC from five healthy subjects were cultured for 7 days, flow cytometric analysis of DNA content was used to assess the mitogenic effect of SEA on PBMC proliferation compared to phytoheamaglutinine (PHA).Quantification of the intracellular viral load showed increased copy number/cell of both or either viral strands after induction with SEA in 18 of 26 patients (69.2%) thus indicating stimulation of viral replication. Flow cytometric analysis showed that mean ± S.D. of percent values of cell proliferation was induced from 3.2 ± 1.5% in un-stimulated cells to 16.7 ± 2.5 % and 16.84 ± 1.7 % in cells stimulated with PHA and SEA respectively.the present study supports earlier reports on SEA proliferative activity on PBMC and provides a strong evidence that the higher morbidity observed in patients co-infected with schistosomiasis and HCV is related, at least in part, to direct stimulation of viral replication by SEA.Hepatitis C Virus (HCV) is the major agent in non A non B hepatitis with serious complications ranging from chronic inflammatory disease to hepatic cirrhosis and end stage liver failure or hepatocellular carcinoma (HCC). It is estimated that 170 millions world wide are infected with HCV. Egypt has unusually high prevalence of hepatitis C resulting in high morbidity and mortality from liver disease. Approximately 20% of blood donors are seropositive for HCV antibodies [1-5]. Schistosomiasis is another hepatotropic infection with a major burden on Egyptian patient population particularly in rural societies[1,6,7]. Co-infections with Schistosoma mansoni were repeatedly shown to augment pathogenesis induced by HBV and HCV hepatitis [2,8,9]. Subjects co-infected with HCV accelerate advancement of liver disease to the chronicity of HCV in
Hepatitis C virus infection and gene expression of hepatocellular carcinoma in Egyptian patients
OSM Ali, MTM Mansour, HT El-Bassyouni, SMA El-Maksoud, RR El-Awady
Egyptian Journal of Medical Human Genetics , 2009,
Abstract: Introduction: Gene expression profiling of hepatocellular carcinoma (HCC) is promising for refining the diagnosis and prognosis as well as identifying potential therapeutic targets. Aim of the Study: Our study aimed to study the gene expression in 40 HCC patients infected with hepatitis C virus (HCV) using RT-PCR technique on surgical liver sample. Gene expression changes in HCV-positive group were compared with gene expression in HCV-negative group. Four genes were included in this study, AFP gene, CD10 gene, HGF gene and GRB2 gene. The expression of the four genes were slightly higher in HCV positive group than in HCV negative group, however, the difference between the two groups was non-significant. HGF gene was expressed in only 20% of HCC patients and GRB2 gene was expressed in 95% of HCC patients. AFP gene and CD10 gene were expressed in all patients. Conclusion: AFP gene, CD10 gene and GRB2 gene play an important role as diagnostic markers of HCC. Key Words: Hepatocellular carcinoma, hepatitis C virus, AFP gene, CD10 gene, HGF gene and GRB2 gene.
Bacterial translocation in an experimental intestinal obstruction model: C-reactive protein reliability?
El-Awady, Saleh Ibrahim;El-Nagar, Mohammed;El-Dakar, Medhat;Ragab, Mohammed;Elnady, Ghada;
Acta Cirurgica Brasileira , 2009, DOI: 10.1590/S0102-86502009000200005
Abstract: background: bacterial translocation occurs in preseptic conditions such as intestinal obstruction through unclear mechanism. the c-reactive protein is an acute phase reactant and a marker of ischemia. methods: 45 albino male rats were divided into 3 groups each 15 rats. gi control, gii simple intestinal-obstruction and giii strangulated obstruction. outcome measures were: (1) bacteriologic count and typing for intestinal contents, intestinal wall, liver, mesenteric lymph nodes and blood (cardiac and portal) (2) histopathologic: mucosal injury score, inflammatory cell infiltrate in the wall, mln, liver, (3) biochemical: serum crp, il-10, mucosal stress pattern (glutathione peroxidase-malonyldialdhyde tissue levels). results: (1) intestinal obstruction associates with bt precursors (bact-overgrowth, mucosal-acidosis, immuno-incomptence), (2) bacterial translocation (frequency and density) was found higher in strangulated i.o, that was mainly enteric (aerobic and anaerobic) and mostly e.coli, (3) the pathogen commonality supports the gut origin hypothesis but the systemic inflammatory response goes with the cytokine generating one. (4) the crp median values for gi, ii, iii were 0.5, 6.9, 8.5 mg/l, for bt +ve 8 mg/l and 0.75 mg/l for bt -ve rats. conclusion: bacterial translocation occurs bi-directional (systemic-portal) in intestinal obstruction and the resultant inflammatory response pathogenesis is mostly 3 hit model. the crp is a non selective marker of suspected i.o cases. however, it is a reliable marker of bt, bt density and vascular compromise during i.o.
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