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Search Results: 1 - 10 of 1962 matches for " Sabine Fevery "
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The Dark Side of EGFP: Defective Polyubiquitination
Mathijs Baens, Heidi Noels, Vicky Broeckx, Sofie Hagens, Sabine Fevery, An D. Billiau, Hugo Vankelecom, Peter Marynen
PLOS ONE , 2006, DOI: 10.1371/journal.pone.0000054
Abstract: Enhanced Green Fluorescent Protein (EGFP) is the most commonly used live cell reporter despite a number of conflicting reports that it can affect cell physiology. Thus far, the precise mechanism of GFP-associated defects remained unclear. Here we demonstrate that EGFP and EGFP fusion proteins inhibit polyubiquitination, a posttranslational modification that controls a wide variety of cellular processes, like activation of kinase signalling or protein degradation by the proteasome. As a consequence, the NF-κB and JNK signalling pathways are less responsive to activation, and the stability of the p53 tumour suppressor is enhanced in cell lines and in vivo. In view of the emerging role of polyubiquitination in the regulation of numerous cellular processes, the use of EGFP as a live cell reporter should be carefully considered.
Identifying Where the Values Come from IT-Innovations in Health and Social Care  [PDF]
Vivian Vimarlund, Sabine Koch
Intelligent Information Management (IIM) , 2012, DOI: 10.4236/iim.2012.425042
Abstract: Studies aimed to capture the effects of IT-innovations in health and social care have shown that there is a gap between expected and factual outcomes. Many decision makers feel the need to articulate an ideal end-state for their organiza-tions. Striking the balance between novelty and believability of such an ideal end-state is often tricky and they become neither satisfied with the ideal not the visioning. In this study, we explore the contribution of IT-innovations to health and social care. The results showed that coherence between context and IT-innovation is important to capture effects and outcomes. Being coherent rather than visionary contributes to identify where you are, as an organization, and to capture effects and outcomes that “make sense” in the context in question. The paper makes an exposition from the model building, algorithm design to performance analysis and contributes to the academic prosperity in Intelligent In-formation Management The knowledge generated is expected to provide input when identifying goals that IT-investments are supposed to achieve.
Ego Depletion After Social Interference  [PDF]
Alex Bertrams, Sabine Pahl
Psychology (PSYCH) , 2014, DOI: 10.4236/psych.2014.51001

The present study examines whether social interference (i.e., interference with one’s goal attainment by the bodily presence of others) depletes the limited resource of self-control strength. In an experimental laboratory study (N = 34), half the participants experienced social interference whereas the other half did not experience social interference by two confederates during a dexterity task. Afterwards, we measured participants’ momentary self-control strength applying a Stroop colour-naming task. In line with our prediction, participants’ performance in the Stroop task indicated that social interference reduced self-control strength. We discuss implications for crowding research and crowding in natural settings.

Culture of Mouse Embryonic Stem Cells with Serum but without Exogenous Growth Factors Is Sufficient to Generate Functional Hepatocyte-Like Cells
Karen Pauwelyn, Philip Roelandt, Tineke Notelaers, Pau Sancho-Bru, Johan Fevery, Catherine M. Verfaillie
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0023096
Abstract: Mouse embryonic stem cells (mESC) have been used to study lineage specification in vitro, including towards a hepatocyte-like fate, and such investigations guided lineage differentiation protocols for human (h)ESC. We recently described a four-step protocol to induce hepatocyte-like cells from hESC which also induced hepatocyte-like cell differentiation of mouse induced pluripotent stem cells. As ESC also spontaneously generate hepatocyte-like cells, we here tested whether the growth factors and serum used in this protocol are required to commit mESC and hESC to hepatocyte-like cells. Culture of mESC from two different mouse strains in the absence of serum and growth factors did not induce primitive streak/definitive endoderm genes but induced default differentiation to neuroectoderm on day 6. Although Activin-A and Wnt3 induced primitive streak/definitive endoderm transcripts most robustly in mESC, simple addition of serum also induced these transcripts. Expression of hepatoblast genes occurred earlier when growth factors were used for mESC differentiation. However, further maturation towards functional hepatocyte-like cells was similar in mESC progeny from cultures with serum, irrespective of the addition of growth factors, and irrespective of the mouse strain. This is in contrast to hESC, where growth factors are required for specification towards functional hepatocyte-like cells. Culture of mESC with serum but without growth factors did not induce preferential differentiation towards primitive endoderm or neuroectoderm. Thus, although induction of primitive streak/definitive endoderm specific genes and proteins is more robust when mESC are exposed to a combination of serum and exogenous growth factors, ultimate generation of hepatocyte-like cells from mESC occurs equally well in the presence or absence of exogenous growth factors. The latter is in contrast to what we observed for hESC. These results suggest that differences exist between lineage specific differentiation potential of mESC and hESC, requiring optimization of different protocols for ESC from either species.
Low NO bioavailability in CCl4 cirrhotic rat livers might result from low NO synthesis combined with decreased superoxide dismutase activity allowing superoxide-mediated NO breakdown: A comparison of two portal hypertensive rat models with healthy controls
Marc Van de Casteele, Jos F van Pelt, Frederik Nevens, Johan Fevery, Jürg Reichen
Comparative Hepatology , 2003, DOI: 10.1186/1476-5926-2-2
Abstract: Endothelial nitric oxide synthase was the solely detected isoform by Western blotting in all livers. In cirrhotic livers, the amount of endothelial nitric oxide synthase protein was lower than in healthy controls, although an overlap existed. Levels of caveolin-1 messenger RNA were within the normal range but endothelin-1 messenger RNA levels were significantly higher in cirrhotic livers (p < 0.05). A markedly lower superoxide dismutase activity was observed in cirrhotic livers as compared to healthy controls (p < 0.05).In contrast to prehepatic portal hypertension, cirrhotic livers had decreased endothelial nitric oxide synthase protein and enhanced endothelin-1 messenger RNA amount. We hypothesise that a vasodilator/vasoconstrictor imbalance may be further aggravated by the reduced activity of superoxide dismutase. Decreased activity allows enhanced superoxide action, which may lead to breakdown of nitric oxide in liver sinusoids.The balance of vasoactive substances in cirrhotic livers is in favour of vasoconstrictors [1-3]. This contrasts with splanchnic and systemic vasodilatation characteristically seen in this condition [1,2]. Nitric oxide (NO), prostacyclin and carbon monoxide are known intrahepatic vasodilating substances, whereas endothelin-1, superoxide (O2-), angiotensin-II, epinephrine and others act as vasoconstricting agents [1-6]. NO is produced by 3 different nitric oxide synthase (NOS) isoforms: neuronal NOS (nNOS), inducible NOS (iNOS) and endothelial NOS (eNOS) [1]. The latter is in a normal liver clearly present in endothelial cells of portal venules, portal arterioles and central venules, as well as in sinusoidal endothelial cells [7,8]. Other liver cell types such as hepatic stellate [9,10], Kupffer cells [9] or hepatocytes [7,9] do not express eNOS. A diminished hepatic activity of eNOS by about 30–50 % was documented in carbon tetrachloride (CCl4) induced cirrhosis [7-9,11], in biliary fibrosis of the rat [9,12] and in advanced human cirrhosi
Effect of Deep Brain Stimulation on Speech Performance in Parkinson's Disease
Sabine Skodda
Parkinson's Disease , 2012, DOI: 10.1155/2012/850596
Abstract: Deep brain stimulation (DBS) has been reported to be successful in relieving the core motor symptoms of Parkinson's disease (PD) and motor fluctuations in the more advanced stages of the disease. However, data on the effects of DBS on speech performance are inconsistent. While there are some series of patients documenting that speech function was relatively unaffected by DBS of the nucleus subthalamicus (STN), other investigators reported on improvements of distinct parameters of oral control and voice. Though, these ameliorations of single speech modalities were not always accompanied by an improvement of overall speech intelligibility. On the other hand, there are also indications for an induction of dysarthria as an adverse effect of STN-DBS occurring at least in some patients with PD. Since a deterioration of speech function has more often been observed under high stimulation amplitudes, this phenomenon has been ascribed to a spread of current-to-adjacent pathways which might also be the reason for the sporadic observation of an onset of dysarthria under DBS of other basal ganglia targets (e.g., globus pallidus internus/GPi or thalamus/Vim). The aim of this paper is to review and evaluate reports in the literature on the effects of DBS on speech function in PD. 1. Introduction 1.1. Dysarthria in Parkinson’s Disease (PD) Nearly 90% of individuals with Parkinson’s disease (PD) develop voice and speech disorders (dysarthria) in the course of their disease [1]. Affected patients may complain about a quiet or weak voice and about difficulties to get speech started. Further, they often report that they are asked to repeat their words because listeners have difficulties to understand although patients themselves may self-estimate their speech as loud and sufficiently articulated [2]. Dysarthria can emerge at any stage of the disease and worsen in the later stages [3] causing a progressive loss of communication and leading to social isolation. Parkinsonian dysarthria has traditionally been interpreted as manifestation of rigor and hypokinesia on the speech effector organs [4] inducing to a multidimensional motor speech impairment including alterations of speech respiration, phonation, articulation, and prosody. Thus, based upon global clinical impression, hypokinetic dysarthria is characterized by a breathy and harsh voice, monotony of pitch and loudness, reduced stress, variable speech rate with short rushes of speech, and imprecise articulation resulting in a reduction of overall speech intelligibility [5–8]. From the therapeutic point of view, the effect
Global Diversity of Aloricate Oligotrichea (Protista, Ciliophora, Spirotricha) in Marine and Brackish Sea Water
Sabine Agatha
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0022466
Abstract: Oligotrichids and choreotrichids are ciliate taxa contributing to the multi-step microbial food web and episodically dominating the marine microzooplankton. The global diversity and distribution of aloricate Oligotrichea are unknown. Here, the geographic ranges of the 141 accepted species and their synonyms in marine and brackish sea water are analyzed, using hundreds of taxonomical and ecological studies; the quality of the records is simultaneously evaluated. The aloricate Oligotrichea match the moderate endemicity model, i.e., the majority (94) of morphospecies has a wide, occasionally cosmopolitan distribution, while 47 morphospecies show biogeographic patterns: they are restricted to single geographic regions and probably include 12 endemic morphospecies. These endemics are found in the Antarctic, North Pacific, and Black Sea, whereas the “flagship” species Strombidinopsis cercionis is confined to the Caribbean Sea. Concerning genera, again several geographic patterns are recognizable. The species richness is distinctly lower in the southern hemisphere than in the northern, ranging from nine morphospecies in the South Pacific to 95 in the North Atlantic; however, this pattern is probably caused by undersampling. Since the loss of species might affect higher trophical levels substantially, the aloricate Oligotrichea should not any longer be ignored in conservation issues. The ecophysiological diversity is considerably larger than the morphological, and even tops the richness of SSrRNA and ITS haplotypes, indicating that probably more than 83–89% of the diversity in aloricate Oligotrichea are unknown. The huge challenge to discover all these species can only be managed by combining the expertises of morphological taxonomists, molecular biologists, ecologists, and physiologists.
"No remoinho da tendência-espiral": quest?es de estética, literatura e ciências naturais na obra de Goethe
Mainberger, Sabine;
Estudos Avan?ados , 2010, DOI: 10.1590/S0103-40142010000200013
Abstract: in his final years goethe was obsessed by the so-called "spiral tendency". the problem, however, was far from new to him as the versions and variations of curved lines and spirals in goethe's work clearly show. these forms can actually be found at the crossroads of poetry, visual aesthetics (namely of ornaments), and scientific studies. a crucial point of reference for aesthetics in the later 18th century was william hogarth's famous concept and model of the "line of beauty" (1753), which also left its traces in goethe's writings, even in his late period. this study examines his elegy "amyntas" (1799), the essay "fossile bull" (1822), and texts on the metamorphoses of plants and the spiral tendency in vegetation. spiral forms seem to be so fascinating for goethe because, with their manifold functions and meanings, they allow us to cross the borders between different genres and disciplines and to connect different kinds of thinking. this transgressive intellectual activity, which we could call 'transdisciplinary', remains a model for important thinkers of the 20th century, such as paul valéry, walter benjamin or aby warburg.
Adelbert von Chamisso e o narcisismo primário
Parmentier, Sabine;
ágora: Estudos em Teoria Psicanalítica , 2005, DOI: 10.1590/S1516-14982005000200004
Abstract: chamisso was a german writer and poet born in an aristocratic french family emigrated to germany in 1792. this paper, inquiring into chamisso's tale "l'étrange histoire de peter schlemihl", his first issued writing, points to its autobiographical features that anticipate the life of its author even in its precise details. this paper shows how artistic sublimation allowed the writer to succeed in finding for himself a path towards scientific work and literary creation, and emphasizes that this kind of "intellectual acceptation" of the repressed could be seen as equivalent to the result of a psychoanalysis treatment.
,,etwas über Gleise" oder Versuchsanordnung ?ffentlicher Platz: Zu Lichtenbergs Sudelbuchaufzeichnung J 528
Mainberger, Sabine;
Pandaemonium Germanicum , 2010, DOI: 10.1590/S1982-88372010000100002
Abstract: the essay analyses the aphorism j 528 in lichtenberg's sudelbüchern as a model study of elementary questions and interests of the enlightenment, and as an example of the author's specific way of thinking and writing focusing on three aspects: 1) epistemological: lichtenberg's text deals with the relationship between reason and anthropological as well as social factors, offering a kind of genealogy of rational behavior; 2) methodological and poetological: the text is a paradigm of lichtenberg's transferring scientific method to non-scientific subjects; 3) reception: lichtenberg's poetics find their complement in a specific way of reading: the reader has to be as active as the writer and is invited to do similar scientific experiments as does the observer of the public place he reads about. in other notes in the sudelbücher, lichtenberg attends to problems of physiology and psychology of perception as, in the 20th century, they will be a main issue in the theory of gestalt. as lichtenberg's way of thinking and writing is a criticism of self imposed immaturity as well as of dogmatic rationalism, enlightenment itself is enlightened.
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