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Search Results: 1 - 10 of 5116 matches for " SP;Paiva-Cavalcanti "
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Application of the mammalian glyceraldehyde-3-phosphate dehydrogenase gene for sample quality control in multiplex PCR for diagnosis of leishmaniasis
Gon?alves, SC;Régis-da-Silva, CG;Brito, MEFC;Brand?o-Filho, SP;Paiva-Cavalcanti, M;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2012, DOI: 10.1590/S1678-91992012000200009
Abstract: leishmaniasis is a neglected disease endemic in five continents. it is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. molecular methods to detect leishmania are considered alternatives to overcome the limitations presented by conventional methods. the aim of this study was to develop multiplex pcr systems able to detect small amounts of target dna of leishmania infantum and leishmania braziliensis, and the gene coding for glyceraldehyde-3-phosphate dehydrogenase (g3pd) in mammals, enabling quality evaluation of the sample simultaneously with detection of the specific target. the systems created for g3pd recognition were combined with detection systems for l. infantum and l. braziliensis to compose multiplex pcr systems for visceral (mvl) and cutaneous (macl) leishmaniasis diagnosis. the multiplex pcr systems developed were assessed in blood samples from five different species of mammal reservoirs involved in the disease cycle in brazil, and 96 and 52 human samples from patients with suspected visceral leishmaniasis (vl) and cutaneous leishmaniasis (acl), respectively. three g3pd detection systems were created (g3pd1, g3pd2 and g3pd3) with different product sizes, g3pd2 was chosen for the formation of multiplex pcr systems. the two multiplex pcr systems (mvl and macl) were reproducible in all species evaluated. results of test samples (sensitivity, specificity and efficiency) suggest its use in routine diagnosis, research activities in medicine and veterinary medicine. additionally, the systems designed to detect the g3pd gene are capable of combining with other targets used for molecular diagnosis of infectious diseases. concerning leishmaniasis, the multiplex pcr systems can be used in epidemiological studies for the detection of new and classic reservoirs, which may contribute to the reliability of results and development of actions to control the disease.
Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
Paiva-Cavalcanti, M;Regis-da-Silva, CG;Gomes, YM;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2010, DOI: 10.1590/S1678-91992010000400004
Abstract: in recent years, the polymerase chain reaction (pcr) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time pcr (qpcr). data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. the benefits of qpcr in relation to conventional pcr include speed, reproducibility and quantitative ability. in addition to operational advantages, qpcr is more sensitive and reproducible and may replace conventional pcr in diagnostic routines. regarding visceral leishmaniasis, the possibility of deployment of real-time pcr in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients. moreover, the use of a technique that possesses elevated diagnostic sensitivity, and can monitor therapy and prevent relapses promotes broader prospects for the disease control.
Clinical and hematological findings in Leishmania braziliensis-infected dogs from Pernambuco, Brazil Achados clínicos e hematológicos em c es infectados por Leishmania braziliensis de Pernambuco, Brasil
Luciana Aguiar Figueredo,Milena de Paiva-Cavalcanti,Ericka Lima Almeida,Sinval Pinto Brand?o-Filho
Revista Brasileira de Parasitologia Veterinária , 2012,
Abstract: Canine cutaneous leishmaniasis by Leishmania braziliensis is a neglected, but widespread disease of dogs in South America. This paper describes clinical and hematological alterations in 17 L. braziliensis-infected dogs from Brazil. The most common hematological findings were thrombocytopenia (82.4%), anemia (70.6%), low packed cell volume (52.9%) and eosinophilia (41.2%). Twelve (70.6%) dogs displayed at least one evident physical alteration; 11 dogs (64.7%) presented skin lesions, four (23.5%) had weight loss and two (11.8%) onychogryphosis. L. braziliensis-infected dogs present clinical and hematological signs often observed in dogs infected by other pathogens. This indicates that veterinarians and public health workers should not consider the presence of non-specific clinical signs as diagnostic criteria for visceral leishmaniasis in dogs living endemic areas to avoid misdiagnosis and subsequent elimination of dogs infected by L. braziliensis. A leishmaniose cutanea canina causada por Leishmania braziliensis é uma doen a negligenciada, mas disseminada entre c es na America do Sul. Este artigo descreve altera es clínicas e hematológicas em 17 c es infectados por L. braziliensis do Brasil. As altera es hematológicas mais comuns foram trombocitopenia (82,4%), anemia (70,6%), baixo valor de hematócrito (52,9%) e eosinofilia (41,2%). Doze (70,6%) c es apresentaram pelo menos uma altera o física; 11 (64,7%) apresentaram les es cutaneas, quatro (23,5%) perda de peso e dois (11,8%) onicogrifose. C es infectados por L. braziliensis apresentaram altera es clínicas e hematológicas inespecíficas que s o comumente observadas em c es infectados por outros patógenos. Isso indica que veterinários e profissionais de saúde pública n o deveriam considerar a presen a de tais sinais clínicos como critério de diagnóstico para leishmaniose visceral em c es, em áreas endêmicas, no intuito de evitar um diagnóstico equivocado e a subsequente elimina o de c es infectados por L. braziliensis.
O público e o privado na saúde
Cavalcanti Cecília Paiva Neto
Ciência & Saúde Coletiva , 2006,
Isolation of filamentous fungi from public telephones of the Metropolitan region of the city of Recife, PE, Brazil
Coutinho, Flavia Paiva;Cavalcanti, Marilene da Silva;Cordeiro Neto, Francisco;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000200027
Abstract: fungi can inhabit our organism without causing any harm, but they show themselves when the immunological system is compromised. in this study, a survey of the occurrence of filamentous fungi was carried out in public telephones of the metropolitan region of the city of recife, pe, brazil. this showed the public phones as a possible mean of transmission of fungal diseases among humans. samples from the environment, audios, speakers and keyboards were taken at the airport, mall, subway and bus station in the months of october/2003 (dry season) and june/2004 (rainy season), totaling 120 samples. the procedure of identification of species was carried out through conventional taxonomy. thirty-four genera were isolated, totaling 73 species, the majority belonging to the anamorphic fungi (91.78%), followed by the ascomycota (6.85%) and the zygomycota (1.37%). there was no significant difference in the proportion of species between the dry and rainy seasons, with 46 and 53 species identified, respectively. due to the lack of maintenance, or inadequate cleaning of public telephones, the fungi present in these appliances may cause mycosis in the users as these telephones are used by people from different social classes and variable habits, both healthy and immunocompromised.
Filamentous fungi isolated from the rhizosphere of melon plants (Cucumis melo L. cv. Gold Mine) cultivated in soil with organic amendments
Coutinho, Flavia Paiva;Cavalcanti, Maria Auxiliadora de Queiroz;Yano-Melo, Adriana Mayumi;
Acta Botanica Brasilica , 2010, DOI: 10.1590/S0102-33062010000100032
Abstract: rhizosphere soil samples were collected in a semiarid area, in the region of the s?o francisco river valley, petrolina, pernambuco state, brazil, to study the diversity of filamentous fungi in a soil cultivated with melon (cucumis melo l. cv. gold mine) and receiving different organic amendments: treatment 1 (control, without organic compost); t2 (77% coconut fiber, 20% goat manure and 3% k2so4); t3 (10% ricinus communis leaves and stems, 50% pennisetum purpureum leaves and 40% goat manure); t4 (77% coconut fiber, 20% goat manure and 3% termophosphate); t5 (47% pennisetum purpureum leaves, 50% goat manure and 3% k2so4); and t6 (57% pennisetum purpureum leaves, 40% goat manure and 3% termophosphate). fungal isolation was carried out by the serial dilution technique to 1:1000. the sorensen index of similarity, frequency and distribution of the fungi were evaluated. seventy-eight species of filamentous fungi were isolated and identified, plus several basidiomycota (04) and mycelia sterilia (02). the predominant genera were aspergillus and penicillium, with 15 and 13 species, respectively. a greater number of species was found in the sowing period (49), and in relation to the organic fertilization, treatment 6 provided the greatest species diversity (43 species). most of the species are saprobes and only a few are considered to be potential pathogens on melon plants, such as fusarium oxysporum, f. solani and myrothecium roridum.
Phosphate-solubilizing fungi isolated from a semiarid area cultivated with melon (Cucumis melo L. cv. gold mine)
Coutinho, Flavia Paiva;Cavalcanti, Maria Auxiliadora de Queiroz;Yano-Melo, Adriana Mayumi;
Acta Botanica Brasilica , 2011, DOI: 10.1590/S0102-33062011000400020
Abstract: considering that little is known about the occurrence of phosphate-solubilizing fungi from areas cultivated with melon, the phosphate solubilization ability of filamentous fungi isolated in these areas was evaluated. three hundred and eighteen filamentous fungal isolates belonging to 23 genera were evaluated, besides aphyllophorales and mycelia sterilia. from those, 52 were able to solubilize p: aphyllophorales (2), aspergillus (34), penicillium (10) and rhizopus (6). these results will contribute to subsidizing further research regarding the capacity of these fungi to solubilize other sources of phosphate applied to the melon crop, as well as indicate the need for a screening program to select those with higher capacity and potential for solubilization.
Ensino do processo de enfermagem: planejamento e inser??o em matrizes curriculares
Leadebal, Oriana Deyze Correia Paiva;Fontes, Wilma Dias de;Silva, César Cavalcanti da;
Revista da Escola de Enfermagem da USP , 2010, DOI: 10.1590/S0080-62342010000100027
Abstract: the objective of this study was to analyze the approach used to teach the nursing process based on the class plans of courses that evidence the concept and method bases of the nursing process in superior education institutions in a state capital of northeast brazil. results showed that the courses are mostly theoretical, with amendments and concept bases emphasizing the theoretical-philosophical support of the nursing process, as well as on the study of the phases comprising it. furthermore, it was shown there was an expression and prevalence of cognitive and psychomotor objectives and the use of traditional methodology.
Determination of Total Soluble Solids Content (Brix) and pH in Milk Drinks and Industrialized Fruit Juices
Alessandro Leite CAVALCANTI,Klélia Forte de OLIVEIRA,Paula Silva PAIVA,Mariangela Vitoriano RABELO Dias
Pesquisa Brasileira em Odontopediatria e Clínica Integrada , 2006,
Abstract: Objective: To analyze the Total Soluble Solids Content (TSSC) - Brix refratometry and pH values of milk drinks (yogurts and chocolate drink) and fruit juices ready to drink (FJRD). Method: Twenty milk drinks and ten fruit juices were evaluated by random experiment, with 3 repetitions for each sample. The analyis of TSSC were made for Brix refratometry and pH were determined. Results: The TSS content of drinks ranged from 13.26 to 26.30 for milk drinks and 10.23 to 13.53 to ready to drink juice. The maximum and lower values to pH were, respectively, 3.58 and 7.01 for milk drinks and 3.07 and 3.72 to drink juice. Conclusion: The high the concentration of TSSC verified in the milk and juice drinks, associated to a low pH can contribute to the development of decay lesions in case they be consumed in excess by the children.
The technology organization of production in health services: recognizing boundaries and embracing perspective
Diego Bonfada,José Rodolfo Lopes de Paiva Cavalcanti,Dayane Pessoa de Araujo,Jacileide Guimar?es
Revista de Enfermagem UFPE On Line , 2010,
Abstract: Objective: to reflect on the current dynamics of health services focusing on the importance of soft technologies such proposal to break with the curative hegemonic medical model. Methods: it was performed a literature review of author considered a basic reference for the theme. From this, was developed a theoretical discussion of the texts consulted with a view to support the discussion of soft technologies and production of health care. Results: we perceived that health services point to a fragmented practice, curative and appreciate the highly complex procedures, expertise and hard technologies and soft-hard to guarantee the quality of care. Conclusion: it is essential that the technological organization of work in health rescue the prioritization of the use of soft technologies to that can move forward in the consolidation of the quality of a health system. In this sense, universities and health institutions must act mutually pointing to the reality transformation. Descriptors:
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