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Search Results: 1 - 10 of 220 matches for " SD Puthucheary "
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Detection of virulence genes in Malaysian Shigella species by multiplex PCR assay
Kwai Thong, Susan Hoe, SD Puthucheary, Rohani Md Yasin
BMC Infectious Diseases , 2005, DOI: 10.1186/1471-2334-5-8
Abstract: A mPCR assay was designed for the simultaneous detection of chromosomal- and plasmid-encoded virulence genes (set1A, set1B, ial and ipaH) in Shigella spp. One hundred and ten Malaysian strains (1997–2000) isolated from patients from various government hospitals were used. Reproducibility and sensitivity of the assay were also evaluated. Applicability of the mPCR in clinical settings was tested with spiked faeces following preincubation in brain heart infusion (BHI) broth.The ipaH sequence was present in all the strains, while each of the set1A, set1B and ial gene was present in 40% of the strains tested. Reproducibility of the mPCR assay was 100% and none of the non-Shigella pathogens tested in this study were amplified. The mPCR could detect 100 colony-forming units (cfu) of shigellae per reaction mixture in spiked faeces following preincubation.The mPCR system is reproducible, sensitive and is able to identify pathogenic strains of shigellae irrespective of the locality of the virulence genes. It can be easily performed with a high throughput to give a presumptive identification of the causal pathogen.Members of the genus Shigella, namely S. flexneri, S. dysenteriae, S. sonnei and S. boydii have caused and continue to be responsible for mortality and/or morbidity in high risk populations such as children under five years of age, senior citizens, toddlers in day-care centres, patients in custodial institutions, homosexual men and, war- and famine-engulfed people. Yearly episodes of shigellosis globally have been estimated to be 164.7 million and of these, 163.2 million were in developing countries and the remaining in industrialized nations. The mortality rate was approximately 0.7% [1]. A recent study by Lee & Puthucheary [2] on bacterial enteropathogens in childhood diarrhoea in a Malaysian urban hospital showed that Shigella spp. was the third most common bacteria isolated. S. flexneri and S. dysenteriae type 1 infections are usually characterized by frequent pa
Intensive care unit acquired muscle weakness: when should we consider rehabilitation?
Zudin Puthucheary, Nicholas Hart
Critical Care , 2009, DOI: 10.1186/cc7937
Abstract: In this month's issue of Critical Care, Truong and coworkers [1] review the data on skeletal muscle dysfunction after acute critical illness. Increasingly recognized, skeletal muscle weakness can be commonplace in the intensive care unit (ICU) setting, with a single centre study demonstrating that 25% of patients have muscle weakness [2]. In another study of 116 patients [3], reduction in limb strength was associated with respiratory muscle weakness and delayed weaning from mechanical ventilation. These and other data have directed the focus of health care in the UK onto rehabilitation after critical illness, and guidelines by the National Institute of Clinical Excellence (NICE) were recently published [4].Identification and stratification of patients with ICU acquired weakness (AW), who could benefit from rehabilitation, is of fundamental importance. Nonvolitional assessments of muscle strength, using such techniques as magnetic stimulation of peripheral nerves, have provided detailed physiological data that demonstrate significant reductions in muscle strength [5-8]. However, availability of these objective tools for assessment is limited outside the research environment, and consequently they are of limited clinical utility. Other measurements have been proposed, such as hand grip strength, which are easier to perform, but such volitional tests in critically ill patients are difficult to interpret, especially if a borderline low normal result is obtained, because this could indicate weakness, poor motivation or inability to complete the task. Ali and colleagues [9] showed that that handgrip strength can be a predictor of mortality, although this could also be a reflection of critical illness severity. A novel technique to consider that is relatively simple and portable is the use of ultrasound to measure quadriceps cross-sectional area as a nonvolitional surrogate marker of quadriceps strength [10]. Although this has the potential to be a clinical useful tool, th
Book Review: Journeying with the Old Testament.
SD Snyman
Acta Theologica , 2012,
Abstract: Gatti, N & Ossum-Batsa, G 2011. Journeying with the Old Testament. Bern: Peter Lang. (Das Alte Testament im Dialog vol 5). 189pp. Paperback. ISBN 978-3-0343-1006-2
Wanneer 'n teks tekste aanhaal. Mal 1:6-14 as voorbeeld
SD Snyman
Acta Theologica , 2008,
Abstract: The problem posed in this article is a fairly straightforward one: Do Pentateuch traditions occur in Malachi 1:6-14 and if so, how are they used? The problem is approached by searching for quotations, shared terminology, inversions and common themes that occur in both the Pentateuch and Malachi. This investigation reveals that there are mainly three ways in which Pentateuch traditions function in this pericope: The vocabulary or phrases in the text allude to recognisable Pentateuch traditions; interpretations are applied to a new situation and utilised in creative ways (new applications were made applicable to the period in which the prophet lived at the time), and the combination with other known traditions from the Old Testament/Hebrew Bible. (Article text in Afrikaans)
Rethinking the demarcation of Malachi 2:17-3:5
SD Snyman
Acta Theologica , 2011,
Abstract: emarcating a unit remains important for the interpretation and understanding of the particular unit in a (Biblical) book. In the case of the book of Malachi, the majority of scholars adhere to the traditional demarcation of the fourth unit in the book of Malachi (2:17-3:5). Closer investigation reveals that the matter is not as settled as one might think. Other possibilities are also advocated by scholars. Some regard Mal. 2:17-3:12 as a unit. Another possibility is to regard Mal. 2:17 as a unit on its own followed by either Mal. 3:1-5 or 3:1-12 as a separate unit. A third possibility is to view Mal. 2:17-3:6 as a unit. This paper argues for yet another possibility stretching from Mal. 2:17-3:7a. Arguments for this delimitation are given and the impact this new possibility has on the exegesis and eventual meaning of both Mal. 2:17-3:7a and the following unit (Mal. 3:7b-12) is explored.
Communicating hope with one breath
SD Edwards
HTS Theological Studies/Teologiese Studies , 2011,
Abstract: The central thesis of this article was that the phenomenon of hope involves states and stages of consciousness development, which can be enhanced through breath control, meditation, prayer and related practices that have formed the essence of various spiritual healing traditions for millennia. In particular, it was argued that breath control can provide a vital foundation for consciousness transformation and the development of hope. Whilst breath control alone may lead to a state of pure, transcendent and/or cosmic consciousness, the practical theological implications are that its effect of enhancing states and stages of consciousness may be anchored and amplified. This process can take place through further contemplative and intercessory meditation, prayer and related behaviour and will differ between people, groups, contexts, religious and/or spiritual traditions. A particular method of breath control called One Breath, which is associated with pure consciousness and the experience of hope was described. Such an experience typically leads to further spiritual practice, healing and transformation. It was concluded that such ongoing spiritual practice is crucial for improving consciousness development, healing and hope for individuals, societies, planet Earth and the cosmos.
Breath psychotherapy
SD Edwards
Inkanyiso: Journal of Humanities and Social Sciences , 2011,
Abstract: Breath psychotherapy is an approach that makes direct use of the breath in healing. There are many forms of breathbased healing: basic breathing and relaxation methods, with or without the practice of psychological skills such as imagery, centring and concentration; expressive physical and emotional techniques; advanced meditation, prayer and other spiritual exercises. Such an approach has been extolled for millennia in the form of various spiritual, wisdom and healing traditions, including ancestor reverence, Judaism, Hinduism, Buddhism, Taoism, Christianity, and Islam which have holistically equated breath, consciousness, energy and spirit, as well as viewing breath as the vital link between body and mind. This approach distils into a perennial psychology, which recognizes a pre-reflective unity underlying and interlinking the various traditions and forms of breath-based healing and therapy. Accordingly, breath psychotherapy is based on an understanding and healing of the total psyche in the original, holistic meaning of this term. The present article is an appreciative inquiry into more recent forms of breath psychotherapy as promoted and used by modern authors and practitioners. Key Words: Breath-based, psychotherapy, breath therapy, consciousness, spirituality, healing.
Ketogenic diets and physical performance
Phinney SD
Nutrition & Metabolism , 2004,
Abstract: Impaired physical performance is a common but not obligate result of a low carbohydrate diet. Lessons from traditional Inuit culture indicate that time for adaptation, optimized sodium and potassium nutriture, and constraint of protein to 15-25 % of daily energy expenditure allow unimpaired endurance performance despite nutritional ketosis.
ANTIBACTERIAL POTENTIAL OF LEAF EXTRACTS FROM KIRGANELIA RETICULATA BAILL
Shruthi SD
International Journal of Pharmaceutical Research and Development , 2010,
Abstract: The in vitro antibacterial activity of crude methanolic, chloroform and hexane extracts of the leaves of Kirganelia reticulata (Euphorbiaceae) were investigated. Susceptibility of some Gram-negative organisms (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi) and Gram-positive organism (Staphylococcus aureus) were tested. Agar well diffusion and broth dilution methods were used to determine the minimum antibacterial activity against all the tested microorganisms. The extracts exhibited antibacterial activities with zones of inhibition ranging from 9.07 - 30.10 mm, 8.17 - 24.57 mm and 5.60 - 14.67 mm for methanol, chloroform and hexane extracts respectively. Screening of crude extracts showed notable minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at concentrations of 100 to 6.25 mgml-1. The organisms were more sensitive to the methanolic extract of the leaves, where as extracts from other solvents like chloroform and hexane showed moderate to weak activity respectively. Similar results have been showed in MIC and MBC.
Effects of GnRH and PGF2a Injection on Progesterone Profile of Postpartum Dairy Cow
SD Rasad
Journal of Animal Production , 2008,
Abstract: A research of progesterone profile on postpartum dairy cows after GnRH and PGF2a treatment had been conducted to determine the skim milk progesterone profiles, which enhance ovarian activity in postpartum dairy cows. It is expected that by this treatment, the interval to first estrus could be reduced, and frequency of estrus and percentage of estrus expression could be increased. Twenty cows were randomly assigned to four treatment groups including Control group, which contain respectively 5 cows each group. Cow (n=5) in group I received 200 mg GnRH i.m. on days 10-14 p.p.; in group II received 15 mg PGF2a on days 20-24 i.m. p.p.; and group III received combination of both. The control group did not receive hormonal treatments. The research used multivariate, completely randomized design, univariate F-test and Duncan’s Multiple Range Test. Results showed that the postpartum interval to first estrus was shorter in treated groups (I, 26 d; II, 22 d, and III, 24 d) as compared to control group (54 d, P< 0.05). The percentage of estrus increased (60%) in group II and III, but no significant differences were found between group I and the control group (40%). Our result suggested that the combination treatment of GnRH and PGF2a (GnRH + PGF2α) enhanced activity in the postpartum cows, resulting in improved reproductive performance. (Animal Production 10(1): 16-21 (2008) Key Words: GnRH, PGF2a , progesterone profile, postpartum dairy cow
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