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Search Results: 1 - 10 of 325129 matches for " S;Daduang "
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Characterization of the allergen Sol gem 2 from the fire ant venom, Solenopsis geminata
Sukprasert, S;Uawonggul, N;Jamjanya, T;Thammasirirak, S;Daduang, J;Daduang, S;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2012, DOI: 10.1590/S1678-91992012000300010
Abstract: sol i 2 is a potent allergen in solenopsis invicta venom, and most humans exhibit reactivity to it. the sol gem 2 allergen found in the venom of the thai tropical fire ant solenopsis geminata was analysed in the present study. the protein was present in higher amounts than other proteins, as determined by sds-page, and presumably has allergenic properties similar to those of sol i 2. sol gem 2 molecular weight is 28 and 15 kda, respectively, under non-reducing and reducing conditions, indicating that its native form is a dimer. lc-ms/ms analysis confirmed its similarity to sol i 2. the mono/dimeric form of sol gem 2 was determined to be relevant by proteomic approach and immunoblotting. an anti-sol gem 2 antibody was produced in mice, with a titer greater than 1:800 according to the western blotting analysis. the sol gem 2-neutralising activity of this antibody was determined in crickets. the paralytic dose 50 (pd50) of crude s. geminata venom was elevated from 0.18 mg/g of body weight to more than 0.90 mg/g of body weight after preincubation with antibody at a ratio of 1:1. these results suggest that sol gem 2 plays an important role in mediating the effects of the piperidine derivatives in the venom.
Antibacterial activity from Siamese crocodile (Crocodylus siamensis) serum
S Preecharram, S Daduang, W Bunyatratchata, T Araki, S Thammasirirak
African Journal of Biotechnology , 2008,
Abstract: Antibacterial agents were purified from Siamese crocodile serum by anion exchange, gel filtration and reversed phase HPLC. Six antibacterial agents designed as Hp14, Hp15, Hp17, Hp31, Hp36 and Hp51 were purified and proved to carry activity against Salmonella typhi, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Vibrio chorelae. The mass analysis of MALDI-TOF for antibacterial agent of Hp14, Hp15 and Hp51 revealed that they are small molecule with a molecular mass less than 1 kDa. The scanning electron microscopy demonstrated that these agents targeted the bacterial membrane and they act like as antimicrobial peptides. The antibacterial agent in the serum may represent the first line of an immune system in a freshwater crocodile.
Genetic analysis of the merozoite surface protein-1 block 2 allelic types in Plasmodium falciparum clinical isolates from Lao PDR
Naly Khaminsou, Onanong Kritpetcharat, Jureerut Daduang, Lertchai Charerntanyarak, Panutas Kritpetcharat
Malaria Journal , 2011, DOI: 10.1186/1475-2875-10-371
Abstract: Plasmodium falciparum isolates were collected from 230 P. falciparum-infected blood samples from three regions of Lao PDR. K1, MAD20 and RO33 were detected by nested PCR; SSCP was used for polymorphism screening. The nested PCR products of each K1, MAD20 and RO33 allelic types that had different banding patterns by SSCP, were sequenced.The overall prevalence of K1, MAD20 and RO33 allelic types in P. falciparum isolates from Lao PDR were 66.95%, 46.52% and 31.30%, respectively, of samples under study. Single infections with K1, MAD20 and RO33 allelic types were 27.83%, 11.74% and 5.22%, respectively; the remainders were multiple clonal infections. Neither parasite density nor age was related to MOI. Sequence analysis revealed that there were 11 different types of K1, eight different types of MAD20, and 7 different types of RO33. Most of them were regional specific, except type 1 of each allelic type was common found in 3 regions under study.Genetic polymorphism with diverse allele types was identified in msp-1 block 2 among P. falciparum clinical isolates in Lao PDR. A rather high level of multiple clonal infections was also observed but the multiplicity of infection was rather low as not exceed 2.0. This basic data are useful for treatment and malaria control program in Lao PDR.Malaria remains one of the most important health-threatening parasitic diseases in tropical and subtropical areas, such as Lao PDR and other countries in Southeast Asia. In Lao PDR, many highly endemic areas exist, especially in the rural areas of Xekong, Attapeu, Savannakhet, Saravane, and Champasack provinces [1-3]. Plasmodium falciparum is responsible for most of the mortality, while Plasmodium vivax causes considerable morbidity [1,3,4]. Despite the enormous efforts that have been directed toward malaria control and prevention, multiple factors-including insecticide resistance in anopheline vectors, the lack of effective vaccines, and the emergence and rapid spread of drug-resistant strai
Antibacterial activity of plasma from crocodile (Crocodylus siamensis) against pathogenic bacteria
Kommanee Jintana,Preecharram Sutthidech,Daduang Sakda,Temsiripong Yosapong
Annals of Clinical Microbiology and Antimicrobials , 2012, DOI: 10.1186/1476-0711-11-22
Abstract: Background The Siamese crocodile (Crocodylus siamensis) is a critically endangered species of freshwater crocodiles. Crocodilians live with opportunistic bacterial infection but normally suffer no adverse effects. They are not totally immune to microbial infection, but their resistance thereto is remarkably effective. In this study, crude and purified plasma extracted from the Siamese crocodile were examined for antibacterial activity against clinically isolated, human pathogenic bacterial strains and the related reference strains. Methods Crude plasma was prepared from whole blood of the Siamese crocodile by differential sedimentation. The crude plasma was examined for antibacterial activity by the liquid growth inhibition assay. The scanning electron microscopy was performed to confirm the effect of crude crocodile plasma on the cells of Salmonella typhi ATCC 11778. Effect of crude crocodile plasma on cell viability was tested by MTT assay. In addition, the plasma was purified by anion exchange column chromatography with DEAE-Toyopearl 650 M and the purified plasma was tested for antibacterial activity. Results Crude plasma was prepared from whole blood of the Siamese crocodile and exhibited substantial antibacterial activities of more than 40% growth inhibition against the six reference strains of Staphylococcus aureus, Salmonella typhi, Escherichia coli, Vibrio cholerae, Pseudomonas aeruginosa, and Staphylococcus epidermidis, and the four clinical isolates of Staphylococcus epidermidis, Pseudomonas aeruginosa, Salmonella typhi, and Vibrio cholerae. Especially, more than 80% growth inhibition was found in the reference strains of Salmonella typhi, Vibrio cholerae, and Staphylococcus epidermidis and in the clinical isolates of Salmonella typhi and Vibrio cholerae. The effect of the crude plasma on bacterial cells of Salmonella typhi, a certain antibacterial material probably penetrates progressively into the cytoplasmic space, perturbing and damaging bacterial membranes. The effect of the crude plasma was not toxic by the yellow tetrazolium bromide (MTT) assay using a macrophage-like cell, RAW 264.7. The pooled four fractions, designated as fractions D1-D4, were obtained by column chromatography, and only fraction D1 showed growth inhibition in the reference strains and the clinical, human pathogenic isolates. Conclusions The crude and purified plasma from the Siamese crocodile significantly showed antibacterial activity against pathogenic bacteria and reference strains by damage cell membrane of target bacterial cells. From the MTT assay, th
Antimicrobial Action of the Cyclic Peptide Bactenecin on Burkholderia pseudomallei Correlates with Efficient Membrane Permeabilization
Kanjana Madhongsa equal contributor,Supaluk Pasan equal contributor,Onanong Phophetleb,Sawinee Nasompag,Sompong Thammasirirak,Sakda Daduang,Suwimol Taweechaisupapong,Andrei L. Lomize,Rina Patramanon
PLOS Neglected Tropical Diseases , 2013, DOI: 10.1371/journal.pntd.0002267
Abstract: Burkholderia pseudomallei is a category B agent that causes Melioidosis, an acute and chronic disease with septicemia. The current treatment regimen is a heavy dose of antibiotics such as ceftazidime (CAZ); however, the risk of a relapse is possible. Peptide antibiotics are an alternative to classical antibiotics as they exhibit rapid action and are less likely to result in the development of resistance. The aim of this study was to determine the bactericidal activity against B. pseudomallei and examine the membrane disrupting abilities of the potent antimicrobial peptides: bactenecin, RTA3, BMAP-18 and CA-MA. All peptides exhibited >97% bactericidal activity at 20 μM, with bactenecin having slightly higher activity. Long term time-kill assays revealed a complete inhibition of cell growth at 50 μM bactenecin and CA-MA. All peptides inhibited biofilm formation comparable to CAZ, but exhibited faster kinetics (within 1 h). Bactenecin exhibited stronger binding to LPS and induced perturbation of the inner membrane of live cells. Interaction of bactenecin with model membranes resulted in changes in membrane fluidity and permeability, leading to leakage of dye across the membrane at levels two-fold greater than that of other peptides. Modeling of peptide binding on the membrane showed stable and deep insertion of bactenecin into the membrane (up to 9 ?). We propose that bactenecin is able to form dimers or large β-sheet structures in a concentration dependent manner and subsequently rapidly permeabilize the membrane, leading to cytosolic leakage and cell death in a shorter period of time compared to CAZ. Bactenecin might be considered as a potent antimicrobial agent for use against B. pseudomallei.
Study of the Structural and Electrical Properties of Cr-Doped BiFeO3 Ceramic  [PDF]
S. S. Arafat, S. Ibrahim
Materials Sciences and Applications (MSA) , 2017, DOI: 10.4236/msa.2017.810051
Abstract: Multiferroic BiFe1-xCrxO3 (x = 0.2 and 0.4) ceramics were synthesized in a single phase. The effects of Cr3+ substitution on the crystal structure, dielectric permittivity and leakage current were investigated. Preliminary X-ray structural studies revealed that the samples had a rhombohedral perovskite crystal structure. The dielectric constant ε' significantly increased while the dielectric loss tanδ was substantially decreased with the increase in Cr3+ substitution. The temperature effect on the dielectric properties exhibited an anomaly corresponding to magneto-electric coupling in the samples and was shifted to lower temperatures with the increase in Cr3+ substitution. The leakage current density also reduced in magnitude with the increase in the Cr3+ substitution.
Degree Splitting of Root Square Mean Graphs  [PDF]
S. S. Sandhya, S. Somasundaram, S. Anusa
Applied Mathematics (AM) , 2015, DOI: 10.4236/am.2015.66086
Abstract: Let \"\" be an injective function. For a vertex labeling f, the induced edge labeling \"\" is defined by, \"\" or \"\"; then, the edge labels are distinct and are from \"\". Then f is called a root square mean labeling of G. In this paper, we prove root square mean labeling of some degree splitting graphs.
A Parameter Estimation Model of G-CSF: Mathematical Model of Cyclical Neutropenia  [PDF]
S. Balamuralitharan, S. Rajasekaran
American Journal of Computational Mathematics (AJCM) , 2012, DOI: 10.4236/ajcm.2012.21002
Abstract: We investigate the FFT (Fast Fourier Transform) model and G-CSF (granulocyte colony-stimulating factor) treatment of CN (Cyclical Neutropenia). We collect grey collies and normal dog’s data from CN and analyze the G-CSF treatment. The model develops the dynamics of circulating blood cells before and after the G-CSF treatment. This is quite natural and useful for the collection of laboratory data for investigation. The proposed interventions are practical. This reduces the quantity of G-CSF required for potential maintenance. This model gives us good result in treatment. The changes would be practical and reduce the risk side as well as the cost of treatment in G-CSF.
Synthesis, Thermal Behaviour, XRD, and Luminescent Properties of Lighter Lanthanidethiodipropionate Hydrates Containing Aminogunidine as Neutral Ligand  [PDF]
S. Packiaraj, S. Govindarajan
Open Journal of Inorganic Chemistry (OJIC) , 2014, DOI: 10.4236/ojic.2014.43006
Aminoguanidine lanthanide thiodipropionate hydrates of composition [Ln(Agun)2(tdp)3·nH2O], Agun = Aminoguanidine, tdp = thiodipropionic acid, where Ln = La, Pr, Nd and Sm if n = 2, have been prepared and characterized by physic-chemical techniques.
Fuzzy Based Intelligent Monitoring of Critical Lines in the Restructured Power Market  [PDF]
S. Rajasekaran, S. Sathiyamoorthy
Circuits and Systems (CS) , 2016, DOI: 10.4236/cs.2016.79191
Abstract: Restructured electric market environment allows the power wheeling transactions between the power producers and customers to meet the growing load demand. This will lead to the possible of congestion in the transmission lines. The possible contingencies of power components further worsen the scenario. This paper describes the methodology for the identification of critical transmission line by computing the real power and reactive power performance indices. It also demonstrates the importance of fuzzy logic technique used to rank the transmission lines according to the severity and demonstrated on IEEE-30 bus system.
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