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Search Results: 1 - 10 of 796760 matches for " S. L. M. Pay?o "
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BLMH and APOE genes in Alzheimer Disease: A possible relation  [PDF]
J. P. B. Ximenez, L. T. Rasmussen, W. A. Orcini, R. W. Labio, G. V. Arruda, P. H. F. Bertolucci, M. A. Smith, S. L. M. Payo
Advances in Alzheimer's Disease (AAD) , 2013, DOI: 10.4236/aad.2013.23015
Abstract: Alzheimer disease (AD) is a progressive and irreversible neurodegenerative disorder that is characterized by cognitive decline, memory loss and confusion. The E4 allele of the apolipoprotein E gene (APOE) is associated with AD and it is the main genetic risk factor for disease. Although the exact physiological function is unknown, bleomycin hydrolase (BLMH) may also be associated with AD development, although previous immunohistochemical findings havebeen inconsistent. Therefore, the purpose ofthis study was to evaluate the genotypic and allele frequencies of theAPOEgene andBLMH1450 G> A polymorphism and assessBLMHexpression using PCR-RFLP and RT-qPCR analyses ofblood samples from patients with Alzheimer disease (AD), healthy elderly adults (EC) andhealthyyoung subjects(YC). BLMHexpression wassignificantly different among groups (p= 0.015) and there was substantial reduction with age and with AD. TheAPOEandBLMHgenotype frequency did not diverge from the Hardy-Weinberg equilibrium. There was a higher frequency of genotype 3/3 inall subjects (61.1%) and the AD group demonstrated a higher frequency of allele 4; however, differences ingenotype and allele distributions were statistically different among groups.

TP53 codon 72 polymorphism as a risk factor for cardiovascular disease in a Brazilian population
Smith, M.A.C.;Silva, M.D.A.;Cendoroglo, M.S.;Ramos, L.R.;Araujo, L.M.Q.;Labio, R.W.;Burbano, R.R.;Chen, E.S.;Payo, S.L.M.;
Brazilian Journal of Medical and Biological Research , 2007, DOI: 10.1590/S0100-879X2006005000174
Abstract: tp53, a tumor suppressor gene, has a critical role in cell cycle, apoptosis and cell senescence and participates in many crucial physiological and pathological processes. identification of tp53 polymorphism in older people and age-related diseases may provide an understanding of its physiology and pathophysiological role as well as risk factors for complex diseases. tp53 codon 72 (tp53:72) polymorphism was investigated in 383 individuals aged 66 to 97 years in a cohort from a brazilian elderly longitudinal study. we investigated allele frequency, genotype distribution and allele association with morbidities such as cardiovascular disease, type ii diabetes, obesity, neoplasia, low cognitive level (dementia), and depression. we also determined the association of this polymorphism with serum lipid fractions and urea, creatinine, albumin, fasting glucose, and glycated hemoglobin levels. dna was isolated from blood cells, amplified by pcr using sense 5'-ttgccgtcccaagcaatggatga-3' and antisense 5'-tctgggaagggacagaagatgac-3' primers and digested with the bstui enzyme. this polymorphism is within exon 4 at nucleotide residue 347. descriptive statistics, logistic regression analysis and student t-test using the multiple comparison test were used. allele frequencies, r (arg) = 0.69 and p (pro) = 0.31, were similar to other populations. genotype distributions were within hardy-weinberg equilibrium. this polymorphism did not show significant association with any age-related disease or serum variables. however, r allele carriers showed lower hdl levels and a higher frequency of cardiovascular disease than p allele subjects. these findings may help to elucidate the physiopathological role of tp53:72 polymorphism in brazilian elderly people.
Frequency of Werner helicase 1367 polymorphism and age-related morbidity in an elderly Brazilian population
Smith, M.A.C.;Silva, M.D.A.;Araujo, L.Q.;Ramos, L.R.;Labio, R.W.;Burbano, R.R.;Peres, C.A.;Andreoli, S.B.;Payo, S.L.M.;Cendoroglo, M.S.;
Brazilian Journal of Medical and Biological Research , 2005, DOI: 10.1590/S0100-879X2005000700008
Abstract: werner syndrome (ws) is a premature aging disease caused by a mutation in the wrn gene. the gene was identified in 1996 and its product acts as a dna helicase and exonuclease. some specific wrn polymorphic variants were associated with increased risk for cardiovascular diseases. the identification of genetic polymorphisms as risk factors for complex diseases affecting older people can improve their prevention, diagnosis and prognosis. we investigated wrn codon 1367 polymorphism in 383 residents in a district of the city of s?o paulo, who were enrolled in an elderly brazilian longitudinal study. their mean age was 79.70 ± 5.32 years, ranging from 67 to 97. this population was composed of 262 females (68.4%) and 121 males (31.6%) of european (89.2%), japanese (3.3%), middle eastern (1.81%), and mixed and/or other origins (5.7%). there are no studies concerning this polymorphism in brazilian population. these subjects were evaluated clinically every two years. the major health problems and morbidities affecting this cohort were cardiovascular diseases (21.7%), hypertension (83.7%), diabetes (63.3%), obesity (41.23%), dementia (8.0%), depression (20.0%), and neoplasia (10.8%). their prevalence is similar to some urban elderly brazilian samples. dna was isolated from blood cells, amplified by pcr and digested with pmaci. allele frequencies were 0.788 for the cysteine and 0.211 for the arginine. genotype distributions were within that expected for the hardy-weinberg equilibrium. female gender was associated with hypertension and obesity. logistic regression analysis did not detect significant association between the polymorphism and morbidity. these findings confirm those from europeans and differ from japanese population.
Controle genético das células-tronco humanas cultivadas
Payo, Spencer L. M.;Segato, Rosimeire;Santos, Ricardo R.;
Revista Brasileira de Hematologia e Hemoterapia , 2009, DOI: 10.1590/S1516-84842009005000021
Abstract: stem cells have a high capacity of self-regeneration, as well as a potential to differentiate into several cell types. these cells can be classified as embryonic or adult. in spite of having inherent properties of stem cells, mesenchymal cells show a certain degree of difficulty to establish cultures. this might induce a loss of the expression of the telomerase enzyme which is considered to be a biological clock or an indicator of the senescence of the cells. the most current and intriguing question for researchers is whether the presumed division potential of cultivated stem cells, over a period of time could result in an accumulation of genetic alterations and consequently, in a neoplastic process. for this reason, cytogenetic techniques are very important to guarantee the control and safety of cultivated stem cells to be used in human therapy. structural chromosomal alterations, such as for example, deletions, translocations and inversions represent an important mechanism by which cells might gradually transform in a neoplastic process. thus, these chromosomal alterations could result in an abnormal expression of the genes and lead to cancer.
Prevalence of Helicobacter pylori cagA and sabA Genotypes in Patients with Gastric Disease  [PDF]
Jéssica Nunes Pereira, Wilson A. Orcini, Rita L. Peruquetti, Marilia A. C. Smith, Spencer L. M. Payo, Lucas T. Rasmussen
Advances in Microbiology (AiM) , 2019, DOI: 10.4236/aim.2019.93017
Abstract: Gastric cancer is one of the most common types of cancer worldwide. Helicobacter pylori is considered one of the most important causes of this condition specially because of its virulence markers as sabA and cagA. Therefore, we aim to investigate the relation between these markers and the gastric diseases in 400 patients who underwent upper digestive endoscopy. To detect the bacteria and its genes by Polymerase Chain Reaction (PCR), the presence of H. pylori was significant when comparing the groups control vs. cancer (p value < 0.0001) OR [95% CI] 12.73 (5.45 - 29.69) and the groups control vs. chronic gastritis (p value < 0.0001) OR [95% CI] 12.99 (7.44 - 22.66). cagA was statistically significant considering its presence when comparing the chronic gastritis vs. cancer groups (p value = 0.0434) OR [95% CI] 2.44 (1.021 - 5.845). Associating both sabA and cagA, we found a statistically significant result (p value < 0.0001) OR [95% CI] 13.68 (3.95 - 47.33) considering the gastritis vs. cancer groups. Helicobacter pylori is directly associated to gastric diseases such as gastritis and cancer and its virulence markers: sabA and cagA increase the injury process to the gastric epithelium making the host more susceptible to cancer.
Agentes causantes de infecciones del torrente circulatorio en ni?os con cáncer, en cinco hospitales de Santiago (1994-1998)
Payá G,Ernesto; Alvarez P,Ana María; Avilés L,Carmen; Cofré G,José; Enríquez O,Nancy; Salgado M,Carmen; Santolaya de P,María Elena; Silva G,Pamela; Tordecilla C,Juan; Varas P,Mónica; Villarroel C,Milena; Zubieta A,Marcela;
Revista médica de Chile , 2001, DOI: 10.4067/S0034-98872001001100009
Abstract: background: pediatric patients in treatment for cancer can have fatal bacterial infections. thus, in the presence of fever or other signs infection, antimicrobials have to be prescribed empirically. aim: to know the causative agents of bacteremia in children with cancer, their changes with time and between different hospitals and their patterns of susceptibility. material and methods: we reviewed the blood cultores of children with cancer in five hospitals of santiago, from 1994 at 1998. results: during the study period, 707 agents were isolated. the most frequently isolated species or genus were coagulase negative staphylococcus (43%), staphylococcus aureus (16%), escherichia coli (9%), klebsiella spp. (8%), pseudomonas spp. (5%) and candida spp. (4%). coagulase negative staphylococcus was 55% resistant to meticilin and s. aureus was 44% resistant. enterobacteriae had 15% resistance to gentamicin and amikacin, 2% to imipenem, 26% to ceftriaxone, 21% to cefotaxim and 20% to ceftazidim. among non fermenting agents resistance was 6% for imipenem, 9% for amikacin 10% for ciprofloxacin, 19% for ceftazidim and 22% for cefoperazone. the resistance of streptococcus spp. (non pneumoniae) to penicillin reached 50% and that of enterococcus spp. was of 33%. conclusions: treatment for pediatric patients with cancer must be modified and new guidelines including more active medications for patients at risk for bacteremia, should be devised (rev méd chile 2001; 129: 1297-1304)
Valoración del pronóstico a corto y largo plazo de pacientes con cirrosis y hemorragia digestiva por hipertensión portal Assessing the short- and long-term prognosis of patients with cirrhosis and acute variceal bleeding
L. Sempere,J. M. Palazón,J. Sánchez-Payá,S. Pascual
Revista Espa?ola de Enfermedades Digestivas , 2009,
Abstract: Objetivo: evaluar la eficacia de los diferentes indicadores pronósticos de supervivencia a corto y largo plazo en pacientes con cirrosis y hemorragia digestiva alta por hipertensión portal (HTP). Material y métodos: calculamos los indicadores pronósticos en una cohorte de 201 pacientes con cirrosis y hemorragia digestiva por HTP ingresados en el Hospital General Universitario de Alicante. Las variables a estudio fueron: edad, sexo, etiología de la cirrosis, hallazgos endoscópicos, episodios previos de hemorragia digestiva por HTP. Infección por el virus de la inmunodeficiencia humana (VIH), hepatocarcinoma (HCC), infección bacteriana durante el episodio de hemorragia digestiva y clasificación de Child-Turcotte-Pugh (CTP) y el modelo para enfermedades terminales del hígado (MELD score) calculados dentro de las primeras 24 horas del inicio de la hemorragia. Los pacientes fueron seguidos al menos 6 meses hasta su muerte, trasplante hepático o final del seguimiento. Resultados: la mediana de seguimiento fue de 66,85 semanas (rango 0-432,4). La mortalidad a las 6 semanas, 3 meses, 12 meses y 36 meses fue de 22,9, 24,9, 34,3 39,8%, respectivamente. La edad ≥ 65 a os, la presencia de HCC, una clasificación de CTP ≥ 10 y un MELD score ≥ 18 fueron las variables asociadas a la mortalidad en el estudio multivariante. La precisión del MELD score como predictor de mortalidad a las 6 semanas, 3 meses, 12 meses y 36 meses fue superior a la de la clasificación de CTP (valor c-estadístico: 6 semanas MELD 0,804, CTP 0,762; 3 meses MELD 0,794, CTP 0,760; 12 meses MELD 0,766, CTP 0,741; 36 meses MELD 0,737, CTP 0,717). Conclusión: el MELD score y la clasificación de CTP, junto con la edad y la presencia de HCC, son marcadores útiles en la valoración pronóstica de supervivencia a corto y largo plazo de los paciente con cirrosis y hemorragia digestiva por HTP. Objective: to evaluate the efficacy of various indicators in predicting short- and long-term survival in patients with cirrhosis and acute variceal bleeding. Material and methods: prognostic indicators were calculated for a cohort of 201 cirrhotic patients with acute variceal bleeding hospitalized in our center, a third-level teaching hospital. The studied variables were: age, sex, etiology of cirrhosis, endoscopic findings, previous variceal bleeding episodes, human immunodeficiency virus (HIV) infection, hepatocellular carcinoma (HCC), infection during episode, and Child-Turcotte-Pugh (CTP) and Model for End-stage Liver Disease (MELD) scores within 24 hours of bleeding onset. Patients were followed up for at least 6
A comparison of two different dosages of somatostatin combined with sclerotherapy for the treatment of acute esophageal variceal bleeding: a prospective randomized trial Comparación de dos dosis diferencias de somatostatina para el tratamiento de sangrado agudo de varices esofágicas: ensayo clínico randomizado
J. M. Palazón,J. Such,J. Sánchez-Payá,L. Company
Revista Espa?ola de Enfermedades Digestivas , 2006,
Abstract: Background: the association of somatostatin (SMT) with endoscopic therapy in patients with cirrhosis and variceal bleeding significantly improves the control of the bleeding episode, and hemodynamic data have shown that a dosage of 500 μg/h allows a more marked reduction of portal pressure versus the usual dosage of 250 μg/h. Aim: to assess if the 500 μg/h dosage is associated with an improved outcome. Methods: sixty-two patients with variceal bleeding were included in the study. Patients were randomized to receive the usual dosage of SMT (group I: 250 μg/h), or a double dosage (group II: 500 μg/h), together with emergency endoscopic sclerotherapy. Results: the control of the bleeding episode was similar in both groups of patients. Early rebleeding was less frequent in patients receiving double vs. single dosage of SMT (p = 0.06). When considering patients with advanced liver disease (Child-Pugh B or C) early rebleeding was significantly less frequent in patients receiving the 500 μg/h dose of SMT (39 vs. 13%, p = 0.03). Conclusions: the perfusion of higher doses of SMT (500 μg/h) in association with emergency sclerotherapy in patients with cirrhosis and esophageal hemorrhage significantly decreases the rate of early rebleeding in patients with more advanced stages of liver disease.
Aerosols in the CALIOPE air quality modelling system: evaluation and analysis of PM levels, optical depths and chemical composition over Europe
S. Basart, M. T. Pay, O. Jorba, C. Pérez, P. Jiménez-Guerrero, M. Schulz,J. M. Baldasano
Atmospheric Chemistry and Physics (ACP) & Discussions (ACPD) , 2012,
Abstract: The CALIOPE air quality modelling system is developed and applied to Europe with high spatial resolution (12 km × 12 km). The modelled daily-to-seasonal aerosol variability over Europe in 2004 is evaluated and analysed. Aerosols are estimated from two models, CMAQv4.5 (AERO4) and BSC-DREAM8b. CMAQv4.5 calculates biogenic, anthropogenic and sea salt aerosol and BSC-DREAM8b provides the natural mineral dust contribution from North African deserts. For the evaluation, we use daily PM10, PM2.5 and aerosol components data from 55 stations of the EMEP/CREATE network and total, coarse and fine aerosol optical depth (AOD) data from 35 stations of the AERONET sun photometer network. Annual correlations between modelled and observed values for PM10 and PM2.5 are 0.55 and 0.47, respectively. Correlations for total, coarse and fine AOD are 0.51, 0.63, and 0.53, respectively. The higher correlations of the PM10 and the coarse mode AOD are largely due to the accurate representation of the African dust influence in the forecasting system. Overall PM and AOD levels are underestimated. The evaluation of the aerosol components highlights underestimations in the fine fraction of carbonaceous matter (EC and OC) and secondary inorganic aerosols (SIA; i.e. nitrate, sulphate and ammonium). The scores of the bulk parameters are significantly improved after applying a simple model bias correction based on the observed aerosol composition. The simulated PM10 and AOD present maximum values over the industrialized and populated Po Valley and Benelux regions. SIA are dominant in the fine fraction representing up to 80% of the aerosol budget in latitudes north of 40° N. In southern Europe, high PM10 and AOD are linked to the desert dust transport from the Sahara which contributes up to 40% of the aerosol budget. Maximum seasonal ground-level concentrations (PM10 > 30 μg m 3) are found between spring and early autumn. We estimate that desert dust causes daily exceedances of the PM10 European air quality limit value (50 μg m 3) in large areas south of 45° N with more than 75 exceedances per year in the southernmost regions.
Allelopathic Effects of Some Selected Tree Species on the Germination and Growth of Cowpea (Vigna unguiculata L. Walp.)  [PDF]
M. O. Aleem, L. O. Alamu, O. S. Olabode
Open Journal of Forestry (OJF) , 2014, DOI: 10.4236/ojf.2014.44037
The allelopathic effect of three tree species (Azardiracta indica, Vitellaria paradoxa, and Parkia bi-globosa) on germination and growth of cowpea was investigated in the Southern Guinea Savannah agro ecological zone of Nigeria. The experiment was laid out in Randomized Complete Block Design (RCBD) with three (3) replicates. Data were collected on germination, plant height, stem diameter, number of leaves, number of branches, root length, the above grand biomass and the below grand biomass and were subjected to statistical analysis using Analysis of Variance (ANOVA) while the significant mean was separated using Duncan’s Multiple Range Test (DMRT) at 5% possibility level. Results showed that the tree species brought about considerable inhibition in the germination of cowpea seeds and in its growth parameters. The statistical germination value of the cowpea seeds under the tree species had decreased value thus indicating that growth inhibitions were seriously felt. It was apparent that Parkia biglobosa (53.33) and Vitellaria paradoxa (60.00) had more inhibitory effect on cowpea seeds germinability than that of Azardiracta indica. (63.33) while all the treatments are lower than that of control (100). The tree species had similar inhibition capability in the cowpea plant height, stem circumference, number of leaves, above grand biomass and below grand biomass. However, in all treatment, statistic showed that there is no significant difference (p < 0.05) among the means.
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