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Search Results: 1 - 10 of 325255 matches for " Sílvia Cufí "
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Different fatty acid metabolism effects of (?)-Epigallocatechin-3-Gallate and C75 in Adenocarcinoma lung cancer
Joana Relat, Adriana Blancafort, Glòria Oliveras, Sílvia Cufí, Diego Haro, Pedro F Marrero, Teresa Puig
BMC Cancer , 2012, DOI: 10.1186/1471-2407-12-280
Abstract: We evaluated in vitro the effects of C75 and EGCG on fatty acid metabolism (FASN and CPT enzymes), cellular proliferation, apoptosis and cell signaling (EGFR, ERK1/2, AKT and mTOR) in human A549 lung carcinoma cells. In vivo, we evaluated their anti-tumour activity and their effect on body weight in a mice model of human adenocarcinoma xenograft.C75 and EGCG had comparable effects in blocking FASN activity (96,9% and 89,3% of inhibition, respectively). In contrast, EGCG had either no significant effect in CPT activity, the rate-limiting enzyme of fatty acid β-oxidation, while C75 stimulated CPT up to 130%. Treating lung cancer cells with EGCG or C75 induced apoptosis and affected EGFR-signaling. While EGCG abolished p-EGFR, p-AKT, p-ERK1/2 and p-mTOR, C75 was less active in decreasing the levels of EGFR and p-AKT. In vivo, EGCG and C75 blocked the growth of lung cancer xenografts but C75 treatment, not EGCG, caused a marked animal weight loss.In lung cancer, inhibition of FASN using EGCG can be achieved without parallel stimulation of fatty acid oxidation and this effect is related mainly to EGFR signaling pathway. EGCG reduce the growth of adenocarcinoma human lung cancer xenografts without inducing body weight loss. Taken together, EGCG may be a candidate for future pre-clinical development.
Circulating fatty acid synthase: an exploratory biomarker to predict efficacy of the dual HER1/HER2 tyrosine kinase inhibitor lapatinib
Cristina Oliveras-Ferraros, Sílvia Cufí, Tamara Sauri-Nadal, Sonia Barco, Bego?a Martin-Castillo, Alejandro Vazquez-Martin, Javier A Menendez
Breast Cancer Research , 2011, DOI: 10.1186/bcr2799
Abstract: AMP-activated protein kinase (AMPK)-activating drugs, by mimicking an elevated AMP/ATP ratio in BC cells, drastically augment the release of extracellular FASN in HER2-positive BC cells [2]. Lapatinib-induced deprivation of tumor cell energy activates AMPK to trigger an entire cascade of metabolic events, including suppression of FASN expression and activity [1,3]. We hypothesized that a differential ability to initiate AMPK-sensed metabolic stress responses may provide information about the efficacy of HER-targeting drugs via changes in the extracellular FASN status. Enzyme-linked immunosorbent assay (ELISA)-based quantitative analyses revealed that lapatinib treatment drastically enhanced extracellular FASN concentration (by at least 8.0-fold) (Figure 1a). Immunoblotting assessment of AMPK phosphorylation at Thr172 confirmed that lapatinib treatment induced a strong activation of AMPK (Figure 1b). A weak but detectable upregulation of PP-AMPKThr172 was observed upon treatment with gefitinib. Trastuzumab, cetuximab, and erlotinib - all of which are unable to promote FASN release - failed to activate AMPK. AMPK knockdown using short interfering RNA (siRNA) transfection [2] fully prevented lapatinib-induced FASN release (Figure 1). Immunoblotting and cell imaging analyses confirmed that FASN was depleted from the cytosol of lapatinib-treated HER2 overexpressors and accumulated in their extracellular milieu (Figure 1).Treatment with lapatinib dramatically increased FASN release (by approximately 17 times) in lapatinib-responsive SKBR3 TzbR cells (Figure 1c), which were selected for long-term outgrowth in trastuzumab-containing culture medium. Extracellular FASN remained unaltered in response to trastuzumab or lapatinib in JIMT-1 BC cells, which exhibit de novo cross-refractoriness to multiple HER1/2-targeted therapies (Figure 1c). Lapatinib treatment significantly activated AMPK and promoted an enormous accumulation of extracellular FASN in lapatinib-hypersensitive MC
A novel inhibitor of fatty acid synthase shows activity against HER2+ breast cancer xenografts and is active in anti-HER2 drug-resistant cell lines
Teresa Puig, Helena Aguilar, Sílvia Cufí, Glòria Oliveras, Carlos Turrado, Sílvia Ortega-Gutiérrez, Bellinda Benhamú, María López-Rodríguez, Ander Urruticoechea, Ramon Colomer
Breast Cancer Research , 2011, DOI: 10.1186/bcr3077
Abstract: In a xenograft model of breast carcinoma cells that are FASN+ and HER2+, we have characterised the anticancer activity and the toxicity profile of G28UCM, the lead compound of a novel family of synthetic FASN inhibitors. In vitro, we analysed the cellular and molecular interactions of combining G28UCM with anti-HER drugs. Finally, we tested the cytotoxic ability of G28UCM on breast cancer cells resistant to trastuzumab or lapatinib, that we developed in our laboratory.In vivo, G28UCM reduced the size of 5 out of 14 established xenografts. In the responding tumours, we observed inhibition of FASN activity, cleavage of poly-ADPribose polymerase (PARP) and a decrease of p-HER2, p- protein kinase B (AKT) and p-ERK1/2, which were not observed in the nonresponding tumours. In the G28UCM-treated animals, no significant toxicities occurred, and weight loss was not observed. In vitro, G28UCM showed marked synergistic interactions with trastuzumab, lapatinib, erlotinib or gefitinib (but not with cetuximab), which correlated with increases in apoptosis and with decreases in the activation of HER2, extracellular signal-regulated kinase (ERK)1/2 and AKT. In trastuzumab-resistant and in lapatinib-resistant breast cancer cells, in which trastuzumab and lapatinib were not effective, G28UCM retained the anticancer activity observed in the parental cells.G28UCM inhibits fatty acid synthase (FASN) activity and the growth of breast carcinoma xenografts in vivo, and is active in cells with acquired resistance to anti-HER2 drugs, which make it a candidate for further pre-clinical development.Fatty acid synthase (FASN) is a multifunctional enzyme that is essential for the endogenous synthesis of long-chain fatty acids from its precursors acetyl-CoA and malonil-CoA [1]. Blocking FASN activity causes cytotoxicity in human cancer cells overexpressing FASN [2-13]. The proposed oncogenic properties of FASN seem to be the result of an increased activation of HER2 and its downstream related phos
Evolutes and isoperimetric deficit in two-dimensional spaces of constant curvature
Julià Cufí,Agustí Reventós
Mathematics , 2014,
Abstract: We relate the total curvature and the isoperimetric deficit of a curve $\gamma$ in a two-dimensional space of constant curvature with the area enclosed by the evolute of $\gamma$. We provide also a Gauss-Bonnet theorem for a special class of evolutes.
Literacy, Ethnicity and Style  [PDF]
Maria Sílvia Cintra
Advances in Anthropology (AA) , 2013, DOI: 10.4236/aa.2013.34A002

I present the result of a two-year research project developed at the “Instituto de Estudos de Linguagem” (IEL/Unicamp, Brazil) and also of action research I have been organizing since 2006 when I started to work as a professor at the “Universidade Federal de Sao Carlos”, in Brazil. Relying on recent developments in New Literacy Studies, I explore the concept of the continuum illiterate-literate and argue that it implies elements of transformation, as well as conservation. I also argue that three intersecting continua must be considered together: the continua oral-written, rural-urban and restricted-full literacy, always taking into consideration ethnicity as a variable (Cohen, 1974). Drawing on ethnographic fieldwork in Brazil, I show how elements of restricted literacy (Goody, 1968) are presented on the threshold of the twenty first century; how they entertain relation with the rural to urban migration and with a marked contrast between different ethnicities; and in what sense this fact may be visible in the everyday use of language and in the style inherent in it.

Nheengatu Language and Its Role in the Tactics of Construction of Indigenous Identity  [PDF]
Maria Sílvia Cintra
Advances in Anthropology (AA) , 2018, DOI: 10.4236/aa.2018.84012
Abstract: Nheengatu is one of the co-official languages of São Gabriel da Cachoeira in the Amazon region (AM/Brazil). About 8000 people in the Upper Rio Negro region speak it, and there exists a contemporary movement for its revitalization in the state of Pará (PA/Brazil). Based upon field research in both these regions, this paper bears reflections on the inter-relations existing between language and identity including references in the areas of Applied Linguistics, Culture Semiotics and Anthropology. I propose a discussion on Nheengatu language, which is often considered in a dysphoric way, i.e., as if it had been imposed on the natives, resulting only from the colonizers’ strategies. I propose we can instead envisage it from the perspective concerning the tactics constructed by the aboriginals in order to preserve and reconquer their identity, i.e., from the point of view of a Poetics of Relation within a complex, contradictory and hybrid linguistic approach.
Bases conceptuales del enfoque histórico-cultural para la comprensión del lenguaje
Orrú,Sílvia Ester;
Estudios pedagógicos (Valdivia) , 2012, DOI: 10.4067/S0718-07052012000200021
Abstract: the following article, as a result of our phd thesis, intends to present the conceptual basis of vigotsky's cultural-historical approach for the comprehension of language, proper to the human species. to understand the individual as a social and cultural being in a developmental history, that goes from interpersonal to intrapersonal, and language being the mediator of all his/her relations. likewise, to highlight the contributions of this approach for the education of people with special needs. to stress that this person is a social individual that develops through social, cultural and historical relations by the mediation of another individual and the existing signs in this mediation, the language being the main sign. we can conclude that, from the cultural-historical approach, a leap is expected in the development of the individual with special needs in the personal relations context, in the teacher's enabling and engaging attitudes and the mediating action of signs.
Histeria e psiquiatria no Brasil da Primeira República
Nunes, Sílvia Alexim;
História, Ciências, Saúde-Manguinhos , 2010, DOI: 10.1590/S0104-59702010000600006
Abstract: the article problematizes brazilian psychiatric thought on hysteria in the early decades of the twentieth century, a time when a biopolicy of population management was lending impetus to the growth of social medicine. this notion is situated within the context of the nineteenth-century debate on hysteria that took place in major european centers and served as the main reference for brazil's fledgling field of psychiatry. the debate found expression in brazilian psychiatric discourse as part of the era's project for medical reform and prevention in brazil. the work of brazilian neurologist ant?nio austregésilo (1876-1960) within this project is also addressed, since he played a fundamental role in dismantling hysteria as a diagnostic category in psychiatry, a process that continued through the entire old republic.
Corpos e ciclos da vida sanumá-yanomami
Guimar?es, Sílvia;
Horizontes Antropológicos , 2010, DOI: 10.1590/S0104-71832010000200012
Abstract: this ethnography focuses on the sanumá, a yanomami subgroup located on the upper auaris river, in the northernmost state of roraima in brazil. it explores central aspects of their vast and complex cosmology by discussing the concept of body and life cycles where special states related with the construction of the person must be observed.
Cuidado formal e informal en Catalu?a: Un análisis de la percepción de las mujeres mayores en relación a sus derechos y obligaciones en materia de cuidados y atención
Etnográfica , 2006,
Abstract: este artículo presenta los resultados de una investigación realizada en catalu?a sobre el cuidado y el sistema de protección social a la dependencia en relación a la gente mayor. en particular versa sobre las expectativas que tienen las personas mayores - mujeres sobre todo - respecto a su propio cuidado, y en qué medida se observan cambios respecto a las generaciones anteriores. contrariamente a lo que podría esperarse, un número significativo de mujeres dirigen sus demandas de atención, no tanto a la familia, cuanto a las instancias públicas. así, encontramos un debilitamiento de la demanda en términos de responsabilidades familiares y un aumento de la demanda de servicios de atención domiciliaria, es decir, un aumento de las demandas en términos de derechos de ciudadanía.
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