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Near Infrared Fluorescence Enhancement by Local Surface Plasmon Resonance from Arrayed Gold Nanoblocks  [PDF]
Fuyuki Ito, Ryuichi Ohta, Yukie Yokota, Kosei Ueno, Hiroaki Misawa, Toshihiko Nagamura
Optics and Photonics Journal (OPJ) , 2013, DOI: 10.4236/opj.2013.31005

The near infrared (NIR) fluorescence enhancement by local surface plasmon resonanoce from arrayed gold (Au) nanoblocks was investigated by NIR fluorescent dyes, IR780, immobilized in hydrophobic DNA thin film on glass substrates, to clarify the gap mode effect on the fluorescence enhancement. In the substrate with Dimer type Au nanoblock arrangement, average total fluorescence intensity was larger by 10.0, 2.4, and 12.4 times for non-polarized, P- and S- polarization as compared with that on a glass substrate alone, respectively. These findings suggested that enhancement of excitation light intensity at nanogap in the Dimer type Au nanoblock arrangement affected the fluorescence intensity. Average total fluorescence intensity, on the other hand, was smaller by 0.63 times as compared with that on a glass substrate alone in the checkerboard type Au array. It is suggested that the fluorescence quenching was caused by the energy transfer from the excited state of IR780 to Au nanoblocks or by the increased deactivation of excited dye molecules induced by resonance with Au nanoblocks at the checkerboard arrangement. We have firstly achieved the NIR fluorescence enhancement by LSPR due to the gap mode.

Voluntary Thigh Muscle Strength with Resection Stump-Dependent Blood Flow and Vasodilation in an Amputated Lower Leg with Total Surface Bearing Prosthesis during Dynamic Knee Extensor: A Case Trial  [PDF]
Takuya Osada, Masahiro Ishiyama, Ryuichi Ueno
Open Journal of Therapy and Rehabilitation (OJTR) , 2019, DOI: 10.4236/ojtr.2019.74011
Abstract: Background: The magnitude of the hyperemic response due to repeated thigh stump exercise on incremental contraction intensity might be useful information in localized exercise tolerance for devising cardiovascular physical therapy for amputees. The effect of exercise on amputated leg blood flow (LBF) may potentially be altered due to voluntary muscle contractions after loss of the lower leg compared with the healthy leg. Case Presentation: A 57-year-old male patient with Burger disease attempted 3 min unilateral repeat/dynamic knee extensor exercise at a target muscle contraction frequency (1 s thigh muscle contraction and 1 s relaxation, 90 repetitions) with each leg at six different contraction intensities (rubber resistance belt). Simultaneous measurement of blood velocity/flow (Doppler ultrasound) in the femoral artery, blood pressure, leg vascular conductance (LVC), and peak muscle strength (PMS) were performed during the 3 min exercise period. The maximum voluntary contraction by one-legged isometric knee muscle contraction was 14.7 kg in non-AL and 7.9 kg in the AL with prosthesis. The relative PMS was defined as “PMS/maximum voluntary contraction × 100 (%)”. Pre-exercise LBF was lower in the AL (200 ± 25 ml/min) than the non-AL (275 ± 74 ml/min). Both the non-AL and AL showed good positive linear relationships between absolute-/relative-PMS and LBF or LVC during 30 s at steady-state before the end of the exercise period. Furthermore, there was also similarity seen in the increase rate in LBF and/or LVC for the incremental relative PMS compared with the absolute PMS.
The Relationship between Athletic Abilities of Japanese High School Soccer Players and Their Psychological Skills in Both Athletic Club and School Life Settings  [PDF]
Kohei Ueno
Advances in Physical Education (APE) , 2014, DOI: 10.4236/ape.2014.42008

To examine the relationship between students’ athletic ability and their psychological skills in the athletic club and the school life setting, 99 players from high school soccer teams were administered with the Psychological Skills Inventory for Athletic Clubs and the Psychological Skills Inventory for School Life. Five coaches of the soccer teams evaluated the players’ athletic ability, using the Technique, Intelligence and Insight, Personality, and Speed (TIPS) evaluation framework. Structural equation modeling was conducted to test a series of structural models. Psychological skills in the school life setting have a direct effect on athletic ability. In addition, psychological skills in an athletic club setting have an indirect effect on athletic ability through psychological skills in the school life setting. In conclusion, the coach’s belief that players who have life skills and can control their daily life must have a high athletic ability was not wrong.

The Effect of Participation in a Game of Tag, an Activity Promoting Helping Behavior, on Children’s Helping Behavior-Related Self-Efficacy  [PDF]
Kohei Ueno
Advances in Physical Education (APE) , 2018, DOI: 10.4236/ape.2018.82021
Abstract: With a breakdown in classroom discipline, bullying and social withdrawal have become social problems in recent years; there is a heightened expectation toward physical education for developing proper social attitudes and cultivating morality. In this study, children were instructed to play “Nakama-oni (helping tag)”, a tag game variant promoting helping behavior through physical education. Thereafter, the frequency of helping behavior in Nakama-oni was checked, and the effect of participation in physical education through Nakama-oni on children’s helping behavior-related self-efficacy was discussed. Sixty elementary school children in the fifth grade played both Nakama-oni and normal tag. The results suggested that: 1) children experienced helping behavior more often in Nakama-oni, compared to normal tag; and 2) participation in Nakama-oni enhanced children’s helping behavior-related self-efficacy. In conclusion, results suggest that incorporating Nakama-oni that promotes helping behavior in physical education enhances children’s helping behavior-related self-efficacy.
Suppression of Methane Gas Emissions and Analysis of the Electrode Microbial Community in a Sediment-Based Bio-Electrochemical System  [PDF]
Yoshiyuki Ueno, Yoji Kitajima
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.45032

The effects of bioelectrochemical systems (BESs) for the suppression of methane gas emissions from sediment were examined using a laboratory-scale reactor system. Methane gas emissions from acetate were suppressed by approximately 36% from control based on the installation of a BES in which carbon-graphite electrodes were buried in sediment and arbitrarily set at certain oxidative potentials (+300 mV vs Ag/AgCl) using a potentiostat. Meanwhile, methane gas emissions increased in the BES reactor where the electrode potential was set at -200 mV. Results obtained from pyrotag sequencing analysis of the microbial community on the surface of the buried electrodes targeting 16S rRNA genes demonstrated that the genusGeobacterhad drastically propagated in a sample from the reactor where the electrodes were buried. Quantitative analysis of 16S rRNA genes of archaea also revealed that the archaeal population had decreased to approximately 1/6 of its original level on the electrode of the BES set at +300 mV. This implied that the oxidation-reduction potential (ORP) in the sediment was raised to the inhibition level for methanogenesis in the vicinity of the buried electrode. Analysis of electron flux in the experiment revealed that electrons intrinsically used for methanogenesis were recovered via current generation in the sediment where a potential of +300 mV was set for the electrode, although most electrons donated from acetate were captured by oxygen respiration and other electron-accepting reactions. These results imply that BES technology is suitable for use as a tool for controlling re-dox-dependent reactions in natural environments, and that it also brought about changes in the microbial population structure and methanogenic activity in sediment.

Methods for the Detection of D-Amino-Acid Oxidase
Konno Ryuichi
Biological Procedures Online , 1998, DOI: 10.1251/bpo7
Abstract: Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.
Elaboration of the Coale-McNeil Nuptiality Model as The Generalized Log Gamma Distribution
Kaneko Ryuichi
Demographic Research , 2003,
Abstract: The Coale-McNeil nuptiality model is a particular case of the generalized log gamma distribution model. In this paper, we demonstrate that recognition of this connection allows an expansion of the possible applications of the Coale-McNeil model. As examples, we propose a procedure to develop country specific standard schedules, and illustrate the utility in regression analysis (directly and via the competing risk framework). In addition, we employ this identification to enhance the ability of the models with empirical adjustment to trace the trajectory of the lifetime schedule for cohorts which have not completed the process. We illustrate an application to Japanese female cohorts. We also propose an application to fertility projection by modeling the fertility schedule by birth order.
2D Quantum Gravity in the Proper-Time Gauge
Ryuichi Nakayama
Physics , 1993, DOI: 10.1016/0370-2693(94)90023-X
Abstract: A two-loop (cylinder) amplitude of the 2d pure gravity theory is obtained in the proper-time gauge ($g_{00}=1$, $g_{01}=g_{10}=0$) in the continuum formulation. The constraint $T_{01}=0$ is solved and used to reduce the problem of field theory to that of quantum mechanics. This reduction can also be proved by using a conformal Ward identity. The amplitude depends on the lengths $l_1, l_2$ of the boundaries, the proper time $T$ and a non-negative integer $m$ associated with winding modes around the boundaries.
Third-Order Density Perturbation and One-Loop Power Spectrum in Dark-Energy-Dominated Universe
Ryuichi Takahashi
Physics , 2008, DOI: 10.1143/PTP.120.549
Abstract: We investigate the third-order density perturbation and the one-loop correction to the linear power spectrum in the dark-energy cosmological model. Our main interest is to understand the dark-energy effect on baryon acoustic oscillations in a quasi-nonlinear regime ($k \approx 0.1h$/Mpc). Analytical solutions and simple fitting formulae are presented for the dark-energy model with the general time-varying equation of state $w(a)$. It turns out that the power spectrum coincides with the approximate result based on the EdS (Einstein de-Sitter) model within 1% for $k<0.4h/$Mpc at $z=0$ in the WMAP (Wilkinson Microwave Anisotropy Probe) 5yr best-fitting cosmological model, which suggests that the cosmological dependence is very small.
Quantum Spin Pump in S=1/2 antiferromagnetic chains -Holonomy of phase operators in sine-Gordon theory-
Ryuichi Shindou
Physics , 2003, DOI: 10.1143/JPSJ.74.1214
Abstract: In this paper, we propose the quantum spin pumping in quantum spin systems where an applied electric field ($E$) and magnetic field ($H$) cause a finite spin gap to its critical ground state. When these systems are subject to alternating electromangetic fields; $(E,H)=(\sin\frac{2\pi t}{T},\cos\frac{2\pi t}{T})$ and travel along the {\it{loop}} $\Gamma_{\rm{loop}}$ which encloses their critical ground state in this $E$-$H$ phase diagram, the locking potential in the sine-Gordon model slides and changes its minimum. As a result, the phase operator acquires $2\pi$ holonomy during one cycle along $\Gamma_{\rm{loop}}$, which means that the quantized spin current has been transported through the bulk systems during this adiabatic process. The relevance to real systems such as Cu-benzoate and ${\rm{Yb}}_4{\rm{As}}_3$ is also discussed.
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