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Search Results: 1 - 10 of 105266 matches for " Rui-Wu Yang "
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Effect of acupuncture serum on intracellular Ca2+ concentration in cultured neurons
LI Rui-Wu
Zhong Xi Yi Jie He Xue Bao , 2004,
Abstract: Objective: To study the humoral factor of acupuncture in improving functional activities of the organism. Methods: Neurons of the cerebral cortex from the new born rats were taken to be cultured in a strictly controlled medium. Seven to ten days later, fluorescein-molecular probe Fluo-3AM was used for staining intracellular Ca2+. Ca2+concentration and its changes after application of acupuncture serum were measured by using a laser confocal microscope. Normal serum and acupuncture serum were derived from the normal control rats and the rats undergoing electro-acupuncture of "Baihui (GV 20)", "Zusanli (ST 36)", "Quchi (LI 11)" and "Sanyinjiao (SP 6)" for 2 weeks. Results: After application of normal serum, the intracellular Ca2+concentration increased strikingly immediately at the beginning, and gradually got stable afterwards. When acupuncture serum was added to the culture fluid, Ca2+concentration lowered significantly. Conclusion: These results suggest that bioactive substances in serum collected from rats undergoing acupuncture can affect the intracellular Ca2+ concentration of the cerebral neurons, and the humoral factor may participate in the effect of acupuncture in regulating functional activities of the organism.
Genetic diversity and phylogeny in Hystrix (Poaceae, Triticeae) and related genera inferred from Giemsa C-banded karyotypes
Zhang, Hai-Qin;Yang, Rui-Wu;Zhang, Li;Ding, Chun-Bang;Zeng, Jian;Zhou, Yong-Hong;
Genetics and Molecular Biology , 2009, DOI: 10.1590/S1415-47572009005000053
Abstract: the phylogenetic relationships of 15 taxa from hystrix and the related genera leymus (nsxm), elymus (sth), pseudoroegneria (st), hordeum (h), psathyrostachys (ns), and thinopyrum (e) were examined by using the giemsa c-banded karyotype. the hy. patula c-banding pattern was similar to those of elymus species, whereas c-banding patterns of the other hystrix species were similar to those of leymus species. the results suggest high genetic diversity within hystrix, and support treating hy. patula as e. hystrix l., and transferring hy. coreana, hy. duthiei ssp. duthiei and hy. duthiei ssp. longearistata to the genus leymus. on comparing c-banding patterns of elymus species with their diploid ancestors (pseudoroegneria and hordeum), there are indications that certain chromosomal re-arrangements had previously occurred in the st and h genomes. furthermore, a comparison of the c-banding patterns of the hystrix and leymus species with the potential diploid progenitors (psathyrostachys and thinopyrum) suggests that hy. coreana and some leymus species are closely related to the ns genome of psathyrostachys, whereas hy. duthiei ssp. duthiei, hy. duthiei ssp. longearistata and some of the leymus species have a close relationship with the e genome. the results suggest a multiple origin of the polyploid genera hystrix and leymus.
FISH analysis of 45S rDNA and 5S rDNA genes in Triticum polonicum L. and T. turgidum L. cv. Ailanmai
波兰小麦和矮兰麦45S rDNA和5S rDNA基因位点FISH分析

LIAO Jin-Qiu,YANG Rui-Wu,ZHOU Yong-Hong,Tsujimoto Hisashi,

遗传 , 2007,
Abstract: Using the method of double color fluorescence in situ hybridization (FISH), we had analyzed Triticum polonicum L. and T. turgidum L.cv. Ailanmai with the probes of 45S rDNA and 5S rDNA. The results indicated that there were highly consistent in T. polonicum L. High and T. turgidum L. cv. Ailanmai, both having four 45S rDNA loci and six 5S rDNA loci. In T. polonicum L. Dwarf, there were also four 45S rDNA loci, the same as that in T. polonicum L. High and T. turgidum L. cv. Ailanmai, but there were eight 5S rDNA loci. At the same time, we discussed the reason of interspecific and intraspecific variation of the two types of rDNA in locus number and location between T. polonicum L. and T. turgidum L. cv. Ailanmai.
Variation of the chromosome number and cytomixis of Houttuynia cordata from China

WU Wei,ZHENG You-Liang,YANG Rui-Wu,CHEN Li,WEI Yu-Ming,

植物分类学报 , 2003,
Abstract: To have a better understanding of the genetic diversity of Houttuynia cordata at the cytological level, the chromosome number and meiosis of pollen mother cells of 90 accessions from China were observed. The species exhibits highly complex variation in chromosome number, with the number varying from 36 to 126, of which 36, 54, 72 and 126 are reported for the first time and the number 81 is the most frequent one. The basic number of this species, the only member of the monotypic genus Houttuynia, might be considered to be 9. Cytomixis was observed for the first time in the species during the diad, tetrad and microspore periods. The chromatin was found to transfer through plasmodesma, conjugation opening and conjugation tube.
Variation of the chromosome number and cytomixis of Houttuynia cordata from China

WU Wei,ZHENG You-L iang,YANG Rui-Wu,CHEN Li,WEI Yu-Ming,

中国科学院研究生院学报 , 2003,
Abstract: To have a better understanding of the genetic diversity of Houttuynia cordata at the cytological level, the chromosome number and meiosis of pollen mother cells of 90 accessions from China were observed. The species exhibits highly complex variation in ch
Evolutionary Dynamics of the Pgk1 Gene in the Polyploid Genus Kengyilia (Triticeae: Poaceae) and Its Diploid Relatives
Xing Fan, Li-Na Sha, Jian Zeng, Hou-Yang Kang, Hai-Qin Zhang, Xiao-Li Wang, Li Zhang, Rui-Wu Yang, Chun-Bang Ding, You-Liang Zheng, Yong-Hong Zhou
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0031122
Abstract: The level and pattern of nucleotide variation in duplicate gene provide important information on the evolutionary history of polyploids and divergent process between homoeologous loci within lineages. Kengyilia is a group of allohexaploid species with the StYP genomic constitutions in the wheat tribe. To investigate the evolutionary dynamics of the Pgk1 gene in Kengyilia and its diploid relatives, three copies of Pgk1 homoeologues were isolated from all sampled hexaploid Kengyilia species and analyzed with the Pgk1 sequences from 47 diploid taxa representing 18 basic genomes in Triticeae. Sequence diversity patterns and genealogical analysis suggested that (1) Kengyilia species from the Central Asia and the Qinghai-Tibetan plateau have independent origins with geographically differentiated P genome donors and diverged levels of nucleotide diversity at Pgk1 locus; (2) a relatively long-time sweep event has allowed the Pgk1 gene within Agropyron to adapt to cold climate triggered by the recent uplifts of the Qinghai-Tibetan Plateau; (3) sweep event and population expansion might result in the difference in the dN/dS value of the Pgk1 gene in allopatric Agropyron populations, and this difference may be genetically transmitted to Kengyilia lineages via independent polyploidization events; (4) an 83 bp MITE element insertion has shaped the Pgk1 loci in the P genome lineage with different geographical regions; (5) the St and P genomes in Kengyilia were donated by Pseudoroegneria and Agropyron, respectively, and the Y genome is closely related to the Xp genome of Peridictyon sanctum. The interplay of evolutionary forces involving diverged natural selection, population expansion, and transposable events in geographically differentiated P genome donors could attribute to geographical differentiation of Kengyilia species via independent origins.
Phylogeny and evolutionary history of Leymus (Triticeae; Poaceae) based on a single-copy nuclear gene encoding plastid acetyl-CoA carboxylase
Xing Fan, Li-Na Sha, Rui-Wu Yang, Hai-Qin Zhang, Hou-Yang Kang, Cun-Bang Ding, Li Zhang, You-Liang Zheng, Yong-Hong Zhou
BMC Evolutionary Biology , 2009, DOI: 10.1186/1471-2148-9-247
Abstract: Two homoeologues of the Acc1 gene were isolated from nearly all the sampled Leymus species using allele-specific primer and were analyzed with those from 35 diploid taxa representing 18 basic genomes in Triticeae. Sequence diversity patterns and genealogical analysis suggested that (1) Leymus is closely related to Psathyrostachys, Agropyron, and Eremopyrum; (2) Psathyrostachys juncea is an ancestral Ns-genome donor of Leymus species; (3) the Xm genome in Leymus may be originated from an ancestral lineage of Agropyron and Eremopyrum triticeum; (4) the Acc1 sequences of Leymus species from the Qinghai-Tibetan plateau are evolutionarily distinct; (5) North America Leymus species might originate from colonization via the Bering land bridge; (6) Leymus originated about 11-12MYA in Eurasia, and adaptive radiation might have occurred in Leymus during the period of 3.7-4.3 MYA and 1.7-2.1 MYA.Leymus species have allopolyploid origin. It is hypothesized that the adaptive radiation of Leymus species might have been triggered by the recent upliftings of the Qinghai-Tibetan plateau and subsequent climatic oscillations. Adaptive radiation may have promoted the rapid speciation, as well as the fixation of unique morphological characters in Leymus. Our results shed new light on our understanding of the origin of Xm genome, the polyploidization events and evolutionary history of Leymus that could account for the rich diversity and ecological adaptation of Leymus species.Leymus Hochst., a polyploid perennial genus in the wheat tribe (Poaceae: Triticeae), includes about 30 species that distribute in a wide range of ecological habitats over the temperate and subtropical and tropic alpine regions [1-3]. The natural habitats of Leymus species range from coastal to inland areas, including saline or alkaline lands, dry or semi-dry areas, as well as shady and moist forests. Morphologically, Leymus species exhibit large variation with absent (L. akmolinensis) to strong rhizomes (L. racemosu
Diet of Non-pollinating Wasps and Their Impact on the Stability of Fig-pollinator Wasp Mutualism

YANG Cheng-yun,WANG Rui-Wu,ZHAO Gui-Fang,YANG Da-Rong,

动物学研究 , 2005,
Abstract: Ficus (Moraceae) and their species-specific pollinator wasps (Agaonidae) form a remarkable plant-insect obligate mutualism, and non-pollinators are the exploiters of the mutualism. The negative impact of exploiters on the reciprocal mutualists might disrupt the reciprocal mutualism in the process of evolution, but how the exploiters could coexist with the reciprocal mutualsits is not still clear. In this study, the diet of the five species of non-pollinators and relationship among fig wasps were analyzed on Ficus racemosa L. in Xishuangbanna from Dec. 2003 to Apr. 2004. In a controlled experiment pollinators and each species of non-pollinators oviposited and counts of wasps and seeds in mature figs were conducted. The results indicated that only Platyneura testacea Motschulsky and Platyneura mayri Rasplus are gall-makers, which can induce the ovaries into galls; Apocrypta sp., Apocrypta westwoodi Grandi and Platyneura agraensis Joseph are the parasitoids. The gall-makers and the parasitoids of pollinators have negative impacts on pollinators, but the impacts are not significant because of the influence of the ants and parasitoids of gall-makers. Additionally, the experiment excluding non-pollinators oviposition showed that the number of offspring of pollinators and seeds were not significantly different with the natural fruits. Moreover, the analysis on the natural population structure of fig wasps revealed that the pollinators are the dominant species. So in the natural condition, the abundance of gall-makers and parasitoids of pollinators are below the level needed to exclude pollinators, and thus they have a relatively weak impact on the stability of fig-pollinator mutualism and can coexist with the mutualism.
PCR-RFLP Analysis on Roegneria, Elymus, Hystrix and Kengyilia in Triticeae (Poaceae)

ZHANG Ying,ZHOU Yong-Hong,ZHANG Li,ZHANG Hai-Qin,YANG Rui-Wu,DING Chun-Bang,

遗传 , 2006,
Abstract: PCR-RFLP of Plasmon on 23 species of Roegneria,Elymus,Hystrix and Kengyilia and 1 outgroup(Triticum aestivum L.) was carried out.The segments amplified with 3 cpDNA and 3 mtDNA universal primers were digested by 15 restriction endonucleases.Among 329 bands produced,304 were polymorphic(92.4%).PCR-RFLP results showed that polymorphisms existed between the four genera and between the species in Roegneria,Elymus,Hystrix and Kengyilia,and genetic similarity coefficient was high.Roegneria grandis,R.aristiglumis,R.elytrigioides,R.alashanica and R.magnicaespes were grouped,while R.caucasica,R.ciliaris,R.amurensis and R.japonensis clustered together.Species of Kengyilia clustered independently.Five species of Elymus,3 of Hystrix and Roegneria kamoji grouped together.The results suggested Kengyilia was a valid genus.As more cytological and molecular data are available,the taxonomic status of Roegneria,Elymus and Hystrix will be recognized.
Karyotypes of six species and two subspecies in Pseudoroegneria and three species in Roegneria

DING Chun-Bang,ZHOU Yong-Hong,ZHENG You-Liang,YANG Rui-Wu,WEI Xiu-Hua,

中国科学院研究生院学报 , 2004,
Abstract: 对拟鹅观草属Pseudoroegneria 6种2亚种和鹅观草属Roegneria 3种植物的核型进行了研究,核型公式如下:P. spicata (Pursh) A. Lve,2n=2x=14=12m (2sat)+2sm; P. strigosa ssp. aegilopoides (Drobov) A. Lve,2n=2x=14=12m (2sat)+2sm; P. libanotica (Hackel) A. Lve,2n=2x=14=10m+4sm (4sat); P. stipifolia
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